scholarly journals Mouse model of metformin-induced diarrhea

2020 ◽  
Vol 8 (1) ◽  
pp. e000898 ◽  
Author(s):  
Hiroshi Takemori ◽  
Akie Hamamoto ◽  
Kenta Isogawa ◽  
Masafumi Ito ◽  
Masanori Takagi ◽  
...  
Keyword(s):  
Quality Of Life ◽  
Animal Model ◽  
Oral Dose ◽  
Evaluation System ◽  
High Water ◽  
High Water Content ◽  
Daily Oral Dose ◽  
Severe Diarrhea ◽  
Wood Creosote

ObjectiveMetformin, an oral medication used for type 2 diabetes mellitus, is the most commonly prescribed drug with less economic burden of patients. Although metformin’s efficacy and safety have long been recognized, approximately 5% of the patients treated with this drug develop severe diarrhea as an adverse effect and have to abandon treatment. Because there is no animal model to study metformin-induced diarrhea, it is hard to develop methods to maintain quality of life of patients prescribed with metformin.Research design and methodsUsing mouse models, we tried to develop an evaluation system for metformin-induced diarrhea to improve diarrheal symptoms in patients with diabetes. Healthy (C57BL/6J) and diabetic obese (db/db) mice were subjected to a stepwise dose escalation of metformin (250 mg/kg/day (125 mg/kg twice daily oral dose)—1000 mg/kg/day (500 mg/kg twice daily oral dose)), and fecal moisture contents and their score were monitored. To evaluate anti-diarrheal medications, wood creosote (a traditional medicine) was tested. Several groups of enterobacteria in fresh feces were examined by using PCR.Results1000 mg/kg/day (four times maximal effective dose) of metformin significantly increased fecal moisture content. Although no symptoms of diarrhea were observed in healthy C57BL/6J mice, the same dose of metformin induced severe diarrhea in diabetic obese db/db mice. A reduction in PCR signals for the Firmicutes group was associated with metformin-induced diarrhea. Wood creosote reduced diarrhea (high water-content) without affecting metformin’s efficacy or enterobacterial flora levels.ConclusionsWe have created the first animal model of metformin-induced diarrhea using db/db mice, which will provide better quality of life for patients suffering from diarrhea caused by metformin.

The Role of Tacrolimus Nanomicelles in Acute Rejection After Liver Transplantation in Rats

2021 ◽  
Vol 21 (2) ◽  
pp. 1061-1069
Author(s):  
Zhantao Xie ◽  
Huibo Zhao ◽  
Yuan Chen ◽  
Sidong Wei ◽  
Jianjun Sun ◽  
...  
Keyword(s):  
Quality Of Life ◽  
Liver Transplantation ◽  
Animal Model ◽  
Acute Rejection ◽  
Rat Liver ◽  
Brown Norway ◽  
Rat Liver Transplantation ◽  
Transplantation Model

Although there is some progress in immunosuppressive therapy of acute rejection, there is still a lack of standardized diagnosis and treatment. For the acute rejection after liver transplantation, there is still a lack of an exact treatment at this stage. Tacrolimus (TAC) side effects will also affect the survival rate and quality of life of recipients after transplantation to a large extent. Rat orthotopic liver transplantation model was established and divided into three groups. In the tolerance group, Brown Norway (BN) to Lewis liver transplantation was used; in the rejection group, Lewis to BN liver transplantation was used; in the TAC group, TAC was injected after operation on the basis of establishing rejection model. The expression of GITRL in Kupffer cells and the change of cytokines were detected 7 days after operation. In this study, the animal model of acute rejection of rat liver transplantation was established to simulate the clinical allogeneic liver transplantation, and the important role of TAC in the acute rejection of rat liver transplantation was evaluated.

Structure, Deposition and Quality of Oyster Shell (Ostrea virginica Gmelin)

1944 ◽  
Vol 6c (3) ◽  
pp. 209-216 ◽  
Author(s):  
J. C. Medcof
Keyword(s):  
Specific Gravity ◽  
Late Summer ◽  
Oyster Shell ◽  
High Water ◽  
High Water Content ◽  
Functional Importance ◽  
Protein Matrix ◽  
Before And After ◽  
Porous Mass

Low quality shells are easily broken, have a high water content and low specific gravity, and contain little pearly and much chalky material. Pearly shell is secreted throughout the season before and after the chalk which it incloses. Chalky areas occur in all shells, have functional importance, are commonest in oysters that are poorest in late summer and are regular in their order of appearance and position on the shell. They are composed of large vertical calcite crystals in a porous mass of smaller crystals with a protein matrix.

scholarly journals PEMANFAATAN LAHAN BEKAS TEMPAT PEMBUANGAN AKHIR DENGAN STABILISASI TANAH SECARA KIMIAWI

2021 ◽  
Vol 4 (1) ◽  
pp. 183
Author(s):  
Satya Bodhinanda ◽  
Aniek Prihatiningsih
Keyword(s):  
Sodium Hydroxide ◽  
Inorganic Material ◽  
Soil Stabilization ◽  
High Water ◽  
Shallow Foundation ◽  
High Water Content ◽  
Structure Model ◽  
Foundation Base ◽  
Load Location

Landfill soil (TPA) has great potential of utilization in construction purpose. Landfill soil has similar characteristic with clay soil that has high water content also contain organic and inorganic material caused by garbage heap. One of the problem in utilizing landfill soil was the low quality of the soil that cause big settlement for the structure. Settlement was an important aspect in performing a construction. Soil stabilization for the landfill soil needed to decrease amount of settlement. Structure model using one, two, three, four floor of load and calculate the settlement occur on the heaviest load location of the foundation. Foundation base was circle and using shallow foundation reconsidering shallow foundation effective for four floor load. Chemical stabilization using 2 methods: Sodium Hydroxide 6% and Limestone 3-4,5%. Based on the calculation, found that Sodium Hydroxide is more effective in decreasing settlement occurred and able to decrease the settlement until 32,42% for the  floor load. However, those methods can’t fulfil the 15 cm settlement limit. Tanah bekas tempat pembuangan akhir (TPA) memiliki potensi besar untuk dimanfaatkan dalam bidang konstruksi. Tanah bekas TPA umumnya memiliki karakteristik serupa dengan tanah lempung berkadar air serta memiliki material organik dan anorganik dari timbunan sampah. Salah satu masalah dalam memanfaatkan tanah bekas TPA adalah rendahnya kualitas tanah yang mengakibatkan penurunan besar pada struktur. Perbaikan pada tanah bekas TPA diperlukan untuk mengurangi besarnya penurunan yang terjadi. Penurunan merupakan aspek penting dalam suatu perancangan konstruksi, maka dari itu batasan pada penurunan ditetapkan untuk menghindari penurunan yang berlebihan yang dapat merusak struktur. Permodelan menggunakan beban struktur 1,2,3, dan 4 lantai dan dihitung penurunan yang terjadi pada fondasi dengan beban struktur terbesar. Penampang fondasi berbentuk lingkaran mempertimbangkan beban percobaan hingga 4 lantai cocok untuk fondasi dangkal. Stabilisasi tanah secara kimiawi menggunakan 2 metode yaitu Sodium Hidroksida 6% dan batu kapur 3-4,5%. Berdasarkan hasil perhitungan, didapati stabilisasi menggunakan Sodium Hidroksida lebih efektif dalam mengurangi penurunan tanah yang terjadi dan mampu mengurangi besarnya penurunan hingga 32,42% pada beban struktur 4 lantai. Namun, stabilisasi tanah menggunakan kedua metode belum dapat mencapai batas aman penurunan sebesar 15 cm.

scholarly journals ANGKA KAPANG DAN KHAMIR PADA LADA PUTIH ASAL BANGKA

2019 ◽  
Vol 5 (2) ◽  
pp. 101
Author(s):  
Oktavio Rosani ◽  
Devy Susanty ◽  
Ary Triyanto
Keyword(s):  
Moisture Content ◽  
Water Content ◽  
High Water ◽  
Quality Standard ◽  
High Water Content ◽  
White Pepper ◽  
Public Consumption ◽  
Mold And Yeast ◽  
The Relationship

Numbers of Mold and Yeast on White Pepper from BangkaWhite pepper is one of Indonesia's spices that are needed for both public consumption and exports. Post-harvest processing of white pepper by farmers is often done with unclean. Each source of white pepper has different water content and will affect the quality of white pepper. In this study, seven samples (S1, S2, S3, S4, S5, S6, S7) were from Bangka. All samples tested had higher water content than SNI quality standard (13%), but still below the quality standard 2 (18%). Sample S2 has the highest water content compared to other samples. Sample S1 and S2 have high Numbers of Mold and Yeast (AKK) and do not suitable with quality standart of BPOM. Of all samples, S2  had the highest AKK (5,51 x 104 colony / g) and sample S5 had the smallest AKK (8,8 x 102 colony / g). This shows the relationship between water content in white pepper with AKK. White pepper that has a high water content has a high AKK, whereas white pepper that has low moisture content has low AKK.Keywords: white pepper, Numbers of Mold and Yeast, moisture contentABSTRAKLada putih adalah salah satu rempah Indonesia  yang  banyak dibutuhkan baik untuk konsumsi masyarakat ataupun ekspor. Proses pengolahan pasca panen lada putih oleh petani sering dilakukan dengan tidak bersih. Setiap sumber lada putih memiliki kadar air yang berbeda dan akan mempengaruhi kualitas lada putih. Pada penelitian ini,tujuh sampel (S1, S2, S3, S4, S5, S6, S7)  berasal dari Bangka. Semua sampel yang di uji memiliki kadar air yang lebih tinggi dari standar mutu 1 SNI (13%), namun masih berada di bawah standar mutu 2 (18 %). Sampel S2 memiliki kadar air yang paling tinggi dibandingkan dengan sampel lainnya. Sampel S1 dan S2 memiliki  (Angka Kapang Khamir) AKK yang tinggi dan tidak memenuhi syarat mutu. BPOM. Dari semua sampel, sampel S2 memiliki AKK paling tinggi (5,51 x 104 koloni/g) dan sampel S5 memiliki AKK paling kecil (8,8 x 102 koloni/g). Hal ini menunjukan hubungan antara kadar air pada lada putih dengan AKK. Lada putih yang memiliki kadar air tinggi memiliki AKK yang juga tinggi, sedangkan lada putih yang memiliki kadar air rendah memiki AKK yang  rendah.Kata Kunci: Lada putih, Angka kapang khamir, Kadar Air

scholarly journals Emetophobia: A case of nausea leading to dehydration in an adolescent female

2020 ◽  
Vol 8 ◽  
pp. 2050313X2095133
Author(s):  
Anju Kannappan ◽  
Amy B Middleman
Keyword(s):  
Treatment Plan ◽  
Healthcare Providers ◽  
High Water ◽  
Specific Phobia ◽  
High Water Content ◽  
Viral Gastroenteritis ◽  
Root Cause ◽  
Diagnostic And Statistical Manual

Emetophobia is defined as the specific fear of vomiting and avoidance behaviors related to vomiting situations; the Diagnostic and Statistical Manual of Mental Disorders, Fifth Edition, specifies this condition as a Specific Phobia: Other type: Vomiting (SPOV). Our case report describes an adolescent presenting with medical complications, specifically notable dehydration, due to new onset Specific Phobia: Other type: Vomiting after experiencing a bout of viral gastroenteritis. In this patient’s case, her severe anxiety served as the root cause for a significant decrease in her quality of life for a year and was not identified by healthcare providers until after an extensive medical workup had taken place. Treatment involved alleviating her dehydration with slow introduction of foods with high water content into her diet and addressing her anxiety with hydroxyzine as a short-term intervention and a selective serotonin reuptake inhibitor for long-term management. This treatment plan allowed for our patient’s symptoms to resolve over the course of a few months and she was able to return to her usual activities of daily living. With discussing emetophobia, this case highlights a topic that is not largely discussed in the literature surrounding adolescent health.

scholarly journals Novel pleural-bladder pump in malignant pleural effusions: from animal model to man

Thorax ◽  
2020 ◽  
Vol 75 (5) ◽  
pp. 432-434 ◽  
Author(s):  
Philippe Astoul ◽  
Sophie Laroumagne ◽  
Jeroen Capel ◽  
Nicholas A Maskell
Keyword(s):  
Quality Of Life ◽  
Animal Model ◽  
Pleural Effusion ◽  
Management Strategy ◽  
Malignant Pleural Effusion ◽  
Pleural Space ◽  
Optimal Management ◽  
Pleural Effusions ◽  
Pump System

Malignant pleural effusion is common and causes disabling symptoms such as breathlessness. Treatments are palliative and centred around improving symptoms and quality of life but an optimal management strategy is yet to be universally agreed. A novel pump system, allowing fluid to be moved from the pleural space to the urinary bladder, may have a role for the management of recurrent malignant pleural effusion. We hereby describe the first animal study using this device and the results of the first application in patients.

scholarly journals Peningkatan Kualitas Minyak Nilam (Pogostemon Cablin Benth) menggunakan Kombinasi Metode Fermentasi, Delignifikasi dan Destilasi

2017 ◽  
pp. 116-121 ◽  
Author(s):  
Salih Muharam ◽  
Lela Mukmilah Yuningsih ◽  
Iim Sulaeman Rohana
Keyword(s):  
Water Content ◽  
Oil Quality ◽  
High Water ◽  
Poor Quality ◽  
Acid Number ◽  
High Water Content ◽  
Pogostemon Cablin ◽  
Combination Of Methods ◽  
Small Capacity

Penyulingan minyak nilam tradisional dilakukan dalam kapasitas kecil dan menggunakanmetode penyulingan sederhanaberdasarkan pada perbedaan titik didih yang jauh atau salah satu komponen bersifat volatil. Proses ini menghasilkan kualitas minyak nilam kurang baik seperti bilangan asam lebih dari 8, minyak berwarna gelap, patchouli alkohol kurang dari 30%, dan kadarair tinggi.Pada penelitian ini telah dilakukan penyulingan minyak dari tanaman nilammenggunakan kombinasi metode yaitu fermentasi-delignifikasi, destilasi dan pemurnian untuk meningkatkan kualitas minyak nilam. Hasil penelitian ini menunjukan bahwa kombinasi proses fermentasi-delignifikasi-destilasi-adsorpsi menghasilkan kualitas minyak nilam yang lebih baik dengan kualitas bilangan asam, kandungan patchouli alkohol,  kadar air dan bobot jenis berturut-turut adalah 7.48, 35.60%, 0.56%,0.0957. The traditional distillationof patchouli oil conducted in small capacity and using a simple distillation method based on differences of wide a boiling point or one component is volatile. This process results a poor quality of patchouli oils such as the acid numbers is more than 8, dark-colored oils, patchouli alcohols contentis less than 30%, and high water content. In this research has been done oil distillation from patchouli plants using a combination of methods i.e. fermentation-delignification, distillation and purification to improve of patchouli oil quality. The results show that the combination of the fermentation-delignification-distillation-adsorptionis better in quality of patchouli oil with the acid number, patchouli alcohol content, water content and density were 7.48, 35.60%, 0.56%, and 0.0957 respectively.

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