A study of DNA protective effect of orange juice supplementation

2013 ◽  
Vol 38 (5) ◽  
pp. 533-536 ◽  
Author(s):  
Yim Tong Szeto ◽  
Tai Lun To ◽  
Sok Cheon Pak ◽  
Wouter Kalle
Keyword(s):  
Hydrogen Peroxide ◽  
Dna Damage ◽  
Vitamin C ◽  
Comet Assay ◽  
Protective Effect ◽  
Orange Juice ◽  
Healthy Subjects ◽  
Venous Blood ◽  
Blood Samples ◽  
And Control

The potential acute genoprotective effect of orange juice supplementation was investigated. Six healthy subjects (aged 33 to 60 years; 3 women and 3 men) were asked to drink 400 mL of commercial orange juice, which contained 100 mg vitamin C and 40.8 g sugar. Venous blood (2 mL) was taken before and 2 h after ingestion (test trial). A week later, the subjects were asked to repeat the trial by drinking 400 mL water with 100 mg vitamin C and 40.8 g glucose (control trial). Lymphocytes isolated from blood samples underwent comet assay on the day of collection. Pre- and postingestion DNA damage scores were measured in both the test and control trials. Results showed that there was a significant decrease in DNA damage induced by hydrogen peroxide after 2 h of supplementation with orange juice, and no change in baseline DNA damage. There was no significant decrease in the DNA damage in lymphocytes in the control trial.

scholarly journals Effect of cryopreservation on DNA damage and DNA repair activity in human blood samples in the comet assay

2021 ◽  
Author(s):  
Ezgi Eyluel Bankoglu ◽  
Franzisca Stipp ◽  
Johanna Gerber ◽  
Florian Seyfried ◽  
August Heidland ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Dna Damage ◽  
Comet Assay ◽  
Human Blood ◽  
Ex Vivo ◽  
Human Biomonitoring ◽  
Dna Damage And Repair ◽  
Blood Samples ◽  
Repair Activity ◽  
Human Blood Samples

AbstractThe comet assay is a commonly used method to determine DNA damage and repair activity in many types of samples. In recent years, the use of the comet assay in human biomonitoring became highly attractive due to its various modified versions, which may be useful to determine individual susceptibility in blood samples. However, in human biomonitoring studies, working with large sample numbers that are acquired over an extended time period requires some additional considerations. One of the most important issues is the storage of samples and its effect on the outcome of the comet assay. Another important question is the suitability of different blood preparations. In this study, we analysed the effect of cryopreservation on DNA damage and repair activity in human blood samples. In addition, we investigated the suitability of different blood preparations. The alkaline and FPG as well as two different types of repair comet assay and an in vitro hydrogen peroxide challenge were applied. Our results confirmed that cryopreserved blood preparations are suitable for investigating DNA damage in the alkaline and FPG comet assay in whole blood, buffy coat and PBMCs. Ex vivo hydrogen peroxide challenge yielded its optimal effect in isolated PBMCs. The utilised repair comet assay with either UVC or hydrogen peroxide-induced lesions and an aphidicolin block worked well in fresh PBMCs. Cryopreserved PBMCs could not be used immediately after thawing. However, a 16-h recovery with or without mitotic stimulation enabled the application of the repair comet assay, albeit only in a surviving cell fraction.

Effect of Intrarectal Prostaglandin E2 Analogue (Enprostil) on Trinitrobenzenesulphonic Acid-Induced Colitis in Rats

2000 ◽  
Vol 28 (1) ◽  
pp. 28-35 ◽  
Author(s):  
Y Onizuka ◽  
K Murase ◽  
H Furusu ◽  
H Isomoto ◽  
Y Mizuta ◽  
...  
Keyword(s):  
Plasma Concentration ◽  
Portal Vein ◽  
Protective Effect ◽  
Colonic Mucosa ◽  
Therapeutic Potential ◽  
Mucosal Damage ◽  
Prostaglandin E ◽  
Once Daily ◽  
Blood Samples ◽  
And Control

Prostaglandins exert a protective effect on colonic mucosa in experimentally induced colitis. This study investigated the effect of enprostil, a prostaglandin E2 (PGE2) analogue, on trinitrobenzenesulphonic acid (TNBS)-induced colitis in rats. Each rat received a rectal enema containing TNBS (30 mg), followed 24 h later by intrarectal once-daily enprostil (200 μg). Enprostil-treated and control rats were killed on day 3 (enprostil group, n = 5; control, n = 6) or day 10 (enprostil group, n = 5; control, n = 5) after TNBS treatment. The area of damaged mucosa of the colon was measured relative to the total colonic area. We also determined the macroscopic score of mucosal damage, and measured PGE2, 6-ketoprostaglandin F1α (6-keto-PGF1α) and thromboxane B2 (TXB2) concentration in portal vein blood samples. Enprostil significantly reduced both the area of damaged mucosa (including the ulcer area) and the macroscopic score after 3 days' treatment compared with control. Similarly, enprostil significantly reduced plasma concentration of PGE2, 6-keto-PGF1α and TXB2 during the acute phase at day 3 of treatment compared with control, but not at day 10. These results suggest that PGE2 enema may have therapeutic potential for treating patients with proctitis or left-sided colitis.

scholarly journals Comparative Study of Vitamin C on Serum Lipid Profile in Healthy Male and Female Human Subjects

2012 ◽  
Vol 4 (3) ◽  
pp. 775-781 ◽  
Author(s):  
G. S. Gaur ◽  
A. K. Dixit
Keyword(s):  
Vitamin C ◽  
Lipid Profile ◽  
Ldl Cholesterol ◽  
Human Subjects ◽  
Test Group ◽  
Control Group ◽  
Blood Samples ◽  
Male And Female ◽  
Vitamin C Supplementation ◽  
And Control

This study aims to assess the comparative effects of vitamin C supplementation on lipid profiles in male and female human subjects. A total of 60 healthy individuals (male and female) were selected randomly, instructed and given the understanding of the purpose of study. The test group comprising  30 individuals  were given 500mg vitamin C tablets one daily for 30 days and control group of 30 individuals were given placebo capsules(glucose 500mg)  one daily for 30 days. Fasting blood samples were collected in the morning for estimation of cholesterol, triglycerides, HDL-C, LDL-C and VLDL-C on first day of the commencement of the study and second blood samples were taken after thirty days of supplementation and same estimations were carried out. Vitamin C caused reduction in serum total cholesterol and LDL cholesterol significantly but it did not have any statistically significant effect on HDL-C, VLDL-C and triglycerides. As far as gender is concerned the effect of vitamin C on lipid profile in males was not significantly different from those in females.© 2012 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved.doi: http://dx.doi.org/10.3329/jsr.v4i3.8894 J. Sci. Res. 4 (3), 775-781 (2012)

scholarly journals Study of DNA damage induced by dental bleaching agents in vitro

2006 ◽  
Vol 20 (1) ◽  
pp. 47-51 ◽  
Author(s):  
Daniel Araki Ribeiro ◽  
Mariângela Esther Alencar Marques ◽  
Daisy Maria Fávero Salvadori
Keyword(s):  
Hydrogen Peroxide ◽  
Dna Damage ◽  
Comet Assay ◽  
Single Cell ◽  
Chinese Hamster ◽  
Dental Bleaching ◽  
Dna Breakage ◽  
The Mean ◽  
Positive Controls

Dental bleaching is a simple and conservative procedure for aesthetic restoration of vital and non-vital discolored teeth. Nevertheless, a number of studies have demonstrated the risk of tissue damage from the contact of these agents with the oral mucosa. In the current study, the genotoxic potential associated with exposure to dental bleaching agents was assessed by the single cell gel (comet) assay in vitro. Chinese hamster ovary (CHO) cells in vitro were exposed to six commercial dental bleaching agents (Clarigel Gold - Dentsply; Whitespeed - Discus Dental; Nite White - Discus Dental; Magic Bleaching - Vigodent; Whiteness HP - FGM and Lase Peroxide - DMC). The results pointed out that all dental bleaching agents tested contributed to DNA damage as depicted by the mean tail moment, being the strongest effect observed with the highest dose of hydrogen peroxide (Whiteness HP and Lase Peroxide, at a 35% concentration). On the other hand, Magic Bleaching (Vigodent) induced the lowest level of DNA breakage. Negative and positive controls displayed absence and presence of DNA-damaging, respectively. Taken together, these results suggest that dental bleaching agents may be a factor that increases the level of DNA damage. A higher concentration of hydrogen peroxide produced higher noxious activities in the genome as detected by single cell gel (comet) assay.

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