Effect of Intrarectal Prostaglandin E2 Analogue (Enprostil) on Trinitrobenzenesulphonic Acid-Induced Colitis in Rats

2000 ◽  
Vol 28 (1) ◽  
pp. 28-35 ◽  
Author(s):  
Y Onizuka ◽  
K Murase ◽  
H Furusu ◽  
H Isomoto ◽  
Y Mizuta ◽  
...  
Keyword(s):  
Plasma Concentration ◽  
Portal Vein ◽  
Protective Effect ◽  
Colonic Mucosa ◽  
Therapeutic Potential ◽  
Mucosal Damage ◽  
Prostaglandin E ◽  
Once Daily ◽  
Blood Samples ◽  
And Control

Prostaglandins exert a protective effect on colonic mucosa in experimentally induced colitis. This study investigated the effect of enprostil, a prostaglandin E2 (PGE2) analogue, on trinitrobenzenesulphonic acid (TNBS)-induced colitis in rats. Each rat received a rectal enema containing TNBS (30 mg), followed 24 h later by intrarectal once-daily enprostil (200 μg). Enprostil-treated and control rats were killed on day 3 (enprostil group, n = 5; control, n = 6) or day 10 (enprostil group, n = 5; control, n = 5) after TNBS treatment. The area of damaged mucosa of the colon was measured relative to the total colonic area. We also determined the macroscopic score of mucosal damage, and measured PGE2, 6-ketoprostaglandin F1α (6-keto-PGF1α) and thromboxane B2 (TXB2) concentration in portal vein blood samples. Enprostil significantly reduced both the area of damaged mucosa (including the ulcer area) and the macroscopic score after 3 days' treatment compared with control. Similarly, enprostil significantly reduced plasma concentration of PGE2, 6-keto-PGF1α and TXB2 during the acute phase at day 3 of treatment compared with control, but not at day 10. These results suggest that PGE2 enema may have therapeutic potential for treating patients with proctitis or left-sided colitis.

Endotoxin-induced prostaglandin E and F release in dogs

1975 ◽  
Vol 228 (2) ◽  
pp. 410-414 ◽  
Author(s):  
FL Anderson ◽  
W Jubiz ◽  
TJ Tsagaris ◽  
H Kuida
Keyword(s):  
Portal Vein ◽  
Acetylsalicylic Acid ◽  
Renal Vein ◽  
Endotoxin Shock ◽  
Significant Rise ◽  
Prostaglandin E ◽  
Hemodynamic Effects ◽  
Vasomotor Tone ◽  
Blood Samples ◽  
Portal Veins

Prostaglandin E and F (PGE and PGF) levels in sequential blood samples obtained simultaneously from the renal and portal veins and aorta during endotoxin shock in dogs were determined by radioimmunoassay. Four groups of dogs were studied. In five control dogs in which no endotoxin was given, PGE and PGF levels did not change significantly at 0, 15, 30, 60, and 90 min. In eight dogs given endotoxin alone, PGE and PGF levels did not change in the aorta. In samples taken from the portal vein there was a significant rise in PGE and PGF 15 min after endotoxin, whereas renal vein PGE and PGF did not become significantly elevated until 60 and 90 min after endotoxin. In six dogs pretreated with acetylsalicylic acid and six dogs pretreated with indomethacin, PGE and PGF levels did not change after endotoxin. Indomethacin modified the delayed hemodynamic effects of endotoxin whereas acetylsalicylic acid did not. Neither drug blocked the immediate hemodynamic effects of endotoxin. Endotoxin-induced PGE and PGF release is probably due to increased synthesis. The mechanism whereby synthesis is stimulated and the extent to which vasomotor tone is influenced by PGE and PGF during endotoxin shock cannot be determined from our data.

A study of DNA protective effect of orange juice supplementation

2013 ◽  
Vol 38 (5) ◽  
pp. 533-536 ◽  
Author(s):  
Yim Tong Szeto ◽  
Tai Lun To ◽  
Sok Cheon Pak ◽  
Wouter Kalle
Keyword(s):  
Hydrogen Peroxide ◽  
Dna Damage ◽  
Vitamin C ◽  
Comet Assay ◽  
Protective Effect ◽  
Orange Juice ◽  
Healthy Subjects ◽  
Venous Blood ◽  
Blood Samples ◽  
And Control

The potential acute genoprotective effect of orange juice supplementation was investigated. Six healthy subjects (aged 33 to 60 years; 3 women and 3 men) were asked to drink 400 mL of commercial orange juice, which contained 100 mg vitamin C and 40.8 g sugar. Venous blood (2 mL) was taken before and 2 h after ingestion (test trial). A week later, the subjects were asked to repeat the trial by drinking 400 mL water with 100 mg vitamin C and 40.8 g glucose (control trial). Lymphocytes isolated from blood samples underwent comet assay on the day of collection. Pre- and postingestion DNA damage scores were measured in both the test and control trials. Results showed that there was a significant decrease in DNA damage induced by hydrogen peroxide after 2 h of supplementation with orange juice, and no change in baseline DNA damage. There was no significant decrease in the DNA damage in lymphocytes in the control trial.

scholarly journals Effects of B. bacteriovorus (ATCC™ 1534) Injection on Some Serum Chemistry Parameters in Rats Injected with P. multocida

2019 ◽  
Vol 1 ◽  
pp. 76-81
Author(s):  
T T Sar ◽  
U E Umeh ◽  
D Ishaleku
Keyword(s):  
Alanine Aminotransferase ◽  
Serum Chemistry ◽  
Sprague Dawley ◽  
Once Daily ◽  
Blood Samples ◽  
Sprague Dawley Rats ◽  
Ketamine Hydrochloride ◽  
And Control ◽  
Final Group

To determine effects of Bdellovibrio bacteriovorus on some serum chemistry parameters, and evaluate its ability to regulate in vivo damage and control pathogen activity, twelve Sprague Dawley rats were injected subcutaneously, once daily, with 1 x 108/ml B. bacteriovorus (ATCC™ 1534) in saline and some serumchemistry parameters measured. Another set of 12 Sprague Dawley rats were also injected once daily with 108/ml of the pathogen P. multocida. Further, 12 other rats were injected with 1 x 108/ml each of B. bacteriovorus and then with P. multocida. All injections were for 168 hours. A group of 12 rats were injected intramuscularly once, at collection of blood samples, with 2 mg/kg of Ketamine Hydrochloride, used as anaesthesia. A final group of 12 rats, not injected with any of the bacteria or anaesthetic served as controls. Blood was collected from all rats for analysis by cardiac puncture. Though there were instances when serum chemistry concentrations were higher on injections with both B. bacteriovorus and P. multocida, compared with rats injected with only P. multocida or the controls, findings showed that B. bacteriovorus injected into rats which had previously been injected with P. multocida led to lower levels of alanine aminotransferase, aspartate aminotransferase and creatinine when compared with concentrations in rats injected with only P. multocida. Mortality was reduced by 88% in rats injected with P. multocida and B. bacteriovorus compared with those injected with only P. multocida. It is concluded that B. bacteriovorus effectively mitigated damage caused by P. multocida in rats.

scholarly journals Protective Effect ofAmphipterygium adstringensExtract on Dextran Sulphate Sodium-Induced Ulcerative Colitis in Mice

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Mario Rodriguez-Canales ◽  
Ruben Jimenez-Rivas ◽  
Maria Margarita Canales-Martinez ◽  
Ana Judith Garcia-Lopez ◽  
Nelly Rivera-Yañez ◽  
...  
Keyword(s):  
Protective Effect ◽  
Colonic Mucosa ◽  
Activity Index ◽  
Wide Spectrum ◽  
Disease Activity Index ◽  
Inflammatory Cells ◽  
Mucosal Damage ◽  
Gastric Ulcers ◽  
Alcoholic Extract ◽  
Cytokine Levels

Amphipterygium adstringensis an endemic species in Mexico commonly known as “cuachalalate.” Healers to treat gastritis, gastric ulcers, and gastrointestinal cancer have traditionally used the bark. We investigated the effects of alcoholic extract ofA. adstringens(AaEE) in DSS-induced colitis in mice. The protective effect of AaEE was determined at 200 mg/kg by oral gavage for 10 days. We determine the effect of AaEE on clinical features (disease activity index), antioxidants, anti-inflammatory, and immunomodulatory activities in relation to the activity of SOD, CAT, and GPx, levels of proinflammatory cytokines, and changes both macroscopic and microscopic of the colonic mucosa. AaEE significantly reduced the inflammation of colon and significantly increased SOD and GPx activities. AaEE also significantly decreased TNF-α, IFN-γ, and IL-1βcytokine levels compared to DSS-treated mice and reduced both infiltration of inflammatory cells and the mucosal damage in colon. The results suggested the protective potential of AaEE in DSS-induced colitis and this might be attributed to its phytochemicals compounds that have been found to induce a wide spectrum of activities such as reduction in oxidative stress, suppression of inflammation, modulating numerous signal transduction pathways, and induction of apoptosis. The findings of this study suggest that AaEE has substantial potential for the treatment of inflammatory colitis.

Serum Endocrine Profile in Relation to Estrus Synchronization in Surti Does

2018 ◽  
Vol 13 (03) ◽  
Author(s):  
M. M. Chaudhary ◽  
C. T. Khasatiya ◽  
S. B. Patel ◽  
S. S. Chaudhary ◽  
V. B. Atara ◽  
...  
Keyword(s):  
Jugular Vein ◽  
Estrus Synchronization ◽  
Progesterone Concentration ◽  
Serum Progesterone ◽  
Blood Samples ◽  
Group Iii ◽  
Group I ◽  
Endocrine Profile ◽  
Group Ii ◽  
And Control

The serum progesterone and estradiol profiles during synchronization of estrus by buck effect and PGF2α treatments were monitored in Surti does. Total eighteen non-pregnant does selected were evenly divided into 3 groups, 6 does in each group. The does of Group I were teased with a sexuallyactive- apronized buck; and those of Group II were treated with PGF2α, i.e., Inj. Lutalyse® @ 7.5 mg/doe IM twice 11 days apart, while the Group III served as untreated control. Blood samples were collected from all the animals on day 0 (before 1st PGF2α injection), 3rd day (during treatment), 11th day (before 2nd PGF2α injection), 14th day (after treatment) and 40th day (post-service) by jugular vein puncture. The serum separated was stored at -20°C till further analysis. In all the three groups, 83.33% does, conceived at first service in the sampling cycle. The overall mean serum progesterone concentration of Group I does (5.82±0.72 ng/ml) was significantly higher (p less than 0.01) as compared to Group II (2.93±0.38 ng/ml) and III (2.88±0.30 ng/ml). Similarly, the overall mean serum progesterone concentration of Surti does on day 0 (2.65±0.46 ng/ml), 3rd (2.56±0.80 ng/ml), 11th (4.45±0.84 ng/ml) and 14th (3.40±0.63 ng/ml) did not differ significantly, but the overall mean level at day 40 (6.31±0.45 ng/ml) was significantly (p less than 0.01) higher, because most of animals became pregnant at that time. The overall mean serum oestradiol-17β levels of Group I (24.40±2.98 pg/ ml) was significantly higher (p less than 0.01) than in Group II (15.77±1.77 pg/ml) and III (12.21±1.45 pg/ ml). On the other hand, the overall mean serum oestradiol-17β levels of Surti does on day 0 (12.89±1.21 pg/ml), 3rd (15.84±1.74 pg/ml), 11th (14.81±1.96 pg/ml), 14th (22.15±2.97 pg/ml) and 40th (21.64±5.16 pg/ml) did not differ significantly (p>0.05) and the slightly higher overall mean level found at 40th day might be the influence of the non-pregnant does at first service in the cumulative animals. The hormonal profile reflected the initiation of cyclicity and establishment of pregnancy in treated and control animals.

scholarly journals Salmonella Bacterin Vaccination Decreases Shedding and Colonization of Salmonella Typhimurium in Pigs

2021 ◽  
Vol 9 (6) ◽  
pp. 1163
Author(s):  
Eduarda Alexandra Gonçalves de Oliveira Moura ◽  
Daniela Gomes da Silva ◽  
Caio Henrique Turco ◽  
Thainara Vitoria Carnevalli Sanches ◽  
Gabriel Yuri Storino ◽  
...  
Keyword(s):  
Salmonella Typhimurium ◽  
Blood Count ◽  
Control Strategies ◽  
Control Group ◽  
Blood Samples ◽  
Commercial Vaccine ◽  
Serum Igg ◽  
And Control ◽  
Experimental Group ◽  
Over Time

Since the occurrence of swine salmonellosis has increased over time and control strategies other than biosecurity are highly recommended, the present study aimed to evaluate the efficacy of vaccination with Salmonella Choleraesuis and Salmonella Typhimurium bacterins in pigs. Two experimental groups were formed: G1, animals immunized with two doses of a commercial vaccine (n = 20); G2, control group (n = 20). After vaccination, all pigs were orally challenged (D0) with 108 CFU of Salmonella Typhimurium and evaluated for 40 days. Every 10 days after D0, five piglets from each experimental group were euthanized and submitted to the necroscopic examination, when organ samples were collected. Blood samples and rectal swabs were collected before the first dose of the vaccine (D−42), before the second dose (D−21), before the challenge (D0), and thereafter, every three days until D39. Blood count, serum IgG measurement by ELISA, and the excretion of Salmonella Typhimurium in feces were evaluated. While the results from blood count and serum IgG concentration did not differ, the detection and excretion of Salmonella between G1 and G2 differed (p < 0.05). Therefore, it was observed that this vaccine partially protected the animals against experimental infection with Salmonella Typhimurium, reducing the excretion of bacteria in feces.

Effect of sialoadenectomy on ethanol-induced gastric mucosal damage in the rat: role of neutrophils

1990 ◽  
Vol 68 (2) ◽  
pp. 207-210 ◽  
Author(s):  
B. L. Tepperman ◽  
B. D. Soper
Keyword(s):  
Growth Factor ◽  
Gastric Mucosa ◽  
Epidermal Growth Factor ◽  
Salivary Glands ◽  
Mucosal Damage ◽  
Gastric Mucosal Damage ◽  
Mucosal Inflammation ◽  
Prostaglandin E ◽  
Epidermal Growth ◽  
Extent Of Damage

We have observed that removal of the salivary glands is associated with an increase in the susceptibility to gastric mucosal damage in the rat. In the present study, we have examined the effect of sialoadenectomy on ethanol-induced mucosal hemorrhagic damage and myeloperoxidase (MPO) activity. Hemorrhagic damage and MPO activity in response to intragastric 50% w/v ethanol were greater in sialoadenectomized rats when compared with sham-operated animals. Pretreatment with 16,16-dimethylprostaglandin E2 (0.3 μg/kg s.c.) reduced damage and MPO activity in both sialoadenectomized and sham control rats receiving 50% ethanol. The reduction in these parameters was greater in control than in sialoadenectomized rats. Pretreatment with epidermal growth factor (5 μg/kg s.c.) significantly reduced MPO activity but did not significantly affect the extent of damage. These data suggest that sialoadenectomy is associated with an increase in mucosal inflammation in animals given ethanol. However, in some situations tissue inflammation (as indicated by MPO activity) was reduced, while the proportion of gastric mucosa exhibiting hemorrhagic damage was not changed.Key words: salivary glands, gastric mucosa, neutrophils, prostaglandin E2, epidermal growth factor.

scholarly journals PHARMACOLOGICAL APPLICATIONS OF MELATONIN

2019 ◽  
Vol 16 (32) ◽  
pp. 214-227
Author(s):  
Rebeca CAPARICA ◽  
Erica Aparecida ROZISCA ◽  
Julio César MACENA ◽  
Laís de Almeida CAMPOS ◽  
Diana Fortkamp GRIGOLETTO
Keyword(s):  
Cell Biology ◽  
Therapeutic Potential ◽  
Biological Activities ◽  
Lipid Peroxides ◽  
Free Radical Scavenger ◽  
Sleep Cycle ◽  
Radical Scavenger ◽  
Signaling Mechanisms ◽  
And Control ◽  
Antioxidant Molecule

Melatonin was discovered by Lerner and Coworkers in 1958, and is the main product secreted by the pineal gland. It is a phylogenetically highly conserved molecule and one of the oldest biological signaling mechanisms. It presents several biological functions, among them the most studied is the regulation of the sleep cycle and wakefulness. In addition, melatonin acts as an immunomodulatory, antioxidant molecule and has anticarcinogenic potential. It also participates in the regulation of mood and control of seasonal reproduction. Melatonin is a potent free radical scavenger and several of its metabolites have the ability to remove singlet oxygen, superoxide radicals, hydroperoxides, hydroxyl radicals and radical lipid peroxides. It easily penetrates cell membranes by being soluble in aqueous and organic media, playing a key role in cell biology. Although their activities are interesting for therapy, their low availability, short half-life, and rapid metabolism restrict their use. In this sense, nanotechnology is a tool that has been studied for the elaboration of systems that improve the pharmacokinetic and pharmacodynamic characteristics of melatonin, in order to potentiate its application in biological models. This review summarizes several studies published in recent years that have shown the most numerous biological activities of melatonin and the improvement of their therapeutic potential through nanotechnology.

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