Morphology and fractal dimension of root systems of maize hybrids bearing the leafy trait

2003 ◽  
Vol 81 (7) ◽  
pp. 706-713 ◽  
Author(s):  
Carlos Costa ◽  
Lianne M Dwyer ◽  
Pierre Dutilleul ◽  
Kayhan Foroutan-pour ◽  
Aiguo Liu ◽  
...  

A rapid and accurate assessment of root morphological characteristics can be important for identifying promising plant genotypes. Scanner-based image analysis has made the measurement of root morphology faster, more accurate, and less subjective, and allows for estimation of fractal dimension (FD). We used scanner-based image analysis to investigate root morphology in leafy vs. conventional maize (Zea mays) genotypes. Two experiments were conducted following a completely randomized design with ten and five replicates. In the first experiment, treatments were factorial combinations of four hybrids (leafy reduced-stature (LRS), non leafy-reduced stature (NLRS), leafy normal stature (LNS), and non leafy normal stature (conventional commercial) hybrid, Pioneer 3979 (P3979)), and four sampling dates (5, 10, 15, and 20 days from transfer to growth pouches). In the second experiment, plants were grown for 15 days from emergence in 10-L plastic pots. This experiment included three maize genotypes (LRS, LNS, and Pioneer 3905 (P3905)). The FD of young root systems was similar for leafy genotypes (FD [Formula: see text] 1.66) and was higher for P3905 (FD [Formula: see text] 1.74). The FD correlated with root characteristics generally included in root growth models (0.68 [Formula: see text] r [Formula: see text] 0.87). Thus, FD could be used to summarize a number of root morphology variables.Key words: root morphology, fractal dimension, root branching, image analysis, maize.

Author(s):  
Debbie Care ◽  
Shirley Nichols ◽  
Derek Woodfield

The use of low-ionic-strength hydroponic culture and image analysis techniques to discriminate and isolate morphologically distinct, genetically differentiated root types within white clover is described. Advantages of this method include the ability to view the genetic expression of the root systems without the modifying effects of growth in soil, to examine the growth and structure of roots over time, and to store the images for further examination. It is recognised that although the root systems grow in three dimensions, they are constrained to two dimensions by the flatbed scanner. However, the morphological parameters determined by image analysis would not be altered whether this analysis was measured in two or three dimensions. Keywords: image analysis, root morphology, solution culture, Trifolium repens


2018 ◽  
Author(s):  
Sarah D. Turner ◽  
Shelby L. Ellison ◽  
Douglas A. Senalik ◽  
Philipp W. Simon ◽  
Edgar P. Spalding ◽  
...  

AbstractCarrot is a globally important crop, yet efficient and accurate methods for quantifying its most important agronomic traits are lacking. To address this problem, we developed an automated analysis platform that extracts components of size and shape for carrot shoots and roots, which are necessary to advance carrot breeding and genetics. This method reliably measured variation in shoot size and shape, leaf number, petiole length, and petiole width as evidenced by high correlations with hundreds of manual measurements. Similarly, root length and biomass were accurately measured from the images. This platform quantified shoot and root shapes in terms of principal components, which do not have traditional, manually-measurable equivalents. We applied the pipeline in a study of a six-parent diallel population and an F2 mapping population consisting of 316 individuals. We found high levels of repeatability within a growing environment, with low to moderate repeatability across environments. We also observed co-localization of quantitative trait loci for shoot and root characteristics on chromosomes 1, 2, and 7, suggesting these traits are controlled by genetic linkage and/or pleiotropy. By increasing the number of individuals and phenotypes that can be reliably quantified, the development of a high-throughput image analysis pipeline to measure carrot shoot and root morphology will expand the scope and scale of breeding and genetic studies.


2016 ◽  
Vol 29 (1) ◽  
pp. 153-162 ◽  
Author(s):  
RAQUEL OLIVEIRA BATISTA ◽  
ANTONIO EDUARDO FURTINI NETO ◽  
SOAMI FERNANDA CAIO DECCETTI ◽  
CASSIANO SILVA VIANA

ABSTRACT: Evaluation of root morphology and kinetic parameters assist in the characterization of genotypes in nutrient uptake efficiency. This study characterized Australian cedar clones concerning their nutrient uptake capacity at the seedling stage through root morphology and the kinetics of P, K, Ca, and Mg uptake. The experiment was conducted using a nutrient solution in a greenhouse and growth chamber. Four Australian cedar clones (HE, XF, XD, and XE), provided by Bela Forest View (Empresa Florestal Bela Vista), were tested using a completely randomized design with five repetitions, totaling 20 experimental plots. The length, volume, surface area, average diameter, and root length per diameter class, as well as the uptake parameters Vmax, Km, Cmin, and the estimated inflow of P, K, Ca, and Mg, were determined. The root morphological characteristics varied between Australian cedar clones; clone XD exhibited the largest root system development. The uptake efficiency of P, K, Ca, and Mg varied between cedar Australian clones. When availability differed, clones XE and XF exhibited greater plasticity in the uptake of P and K respectively, and similar results were found for clone HE in the uptake of Ca and Mg.


2018 ◽  
Author(s):  
Weikun Zhu ◽  
Erfan Mohammadi ◽  
Ying Diao

Morphology modulation offers significant control over organic electronic device performance. However, morphology quantification has been rarely carried out via image analysis. In this work, we designed a MATLAB program to evaluate two key parameters describing morphology of small molecule semiconductor thin films: fractal dimension and film coverage. We then employ this program in a case study of meniscus-guided coating of 2,7-dioctyl[1]benzothieno[3,2-b][1]benzothiophene (C<sub>8</sub>-BTBT) under various conditions to analyze a diverse and complex morphology set. The evolution of morphology in terms of fractal dimension and film coverage was studied as a function of coating speed. We discovered that combined fractal dimension and film coverage can quantitatively capture the key characteristics of C<sub>8</sub>-BTBT thin film morphology; change of these two parameters further inform morphology transition. Furthermore, fractal dimension could potentially shed light on thin film growth mechanisms.


2021 ◽  
pp. 106907
Author(s):  
Lindomar Sousa Brito ◽  
Ana Karina da Silva Cavalcante ◽  
Alexandra Soares Rodrigues ◽  
Priscila Assis Ferraz ◽  
Rodrigo Freitas Bittencourt ◽  
...  

1975 ◽  
Vol 5 (2) ◽  
pp. 171-175 ◽  
Author(s):  
Hugh E. Wilcox ◽  
Ruth Ganmore-Neumann

Seedlings of Pinusresinosa were grown at root temperatures of 16, 21 and 27 °C, both aseptically and after inoculation with the ectendomycorrhizal fungus BDG-58. Growth after 3 months was significantly influenced by the presence of the fungus at all 3 temperatures. The influence of the fungus on root growth was obscured by the effects of root temperature on morphology. The root system at 16 and at 21 °C possessed many first-order laterals with numerous, well developed second-order branches, but those at 27 °C had only a few, relatively long, unbranched first-order laterals. Although the root systems of infected seedlings were larger, the fungus increased root growth in the same pattern as determined by the temperature.


Author(s):  
Paresh Kumar Behera ◽  
Diksha Mohapatra

World Health Organization (WHO) defined the terminology ‘Oral Potentially malignant disorders’ (OPMD) as the presence of risk of malignancy in a lesion or condition either during the time of initial diagnosis or at a future date with the commonly accepted prevalence of 1–5%. All OPMDs may not transform into malignancy, many factors have been explored which effectively assess the risk of malignant transformation in OPMDs including many clinical, pathological and molecular factors. Qualitative & experimental factors of different cells in cytological preparations and/or biopsy specimens are reliable parameters for pathologists. The analysis and assessment of histological units can be enhanced by image analysis assisted by a computer that can be used for statistical comparisons. Fractal geometry is considered to be an ideal method of image analysis in quantitative microscopy & histopathology. Fractal dimension analysis is not only limited to determining cell and tumour types but can also be used for determining cellular behaviours in vitro such as cell migration, apoptosis and cellular differentiation, which can be a useful characterization of oral cancer lines and further help in the treatment planning. Keywords: Oral Cancer, Oral Potentially Malignant Disorders (OPMDs), Conventional morphometry, Fractal Dimension, Photomicrograph


2010 ◽  
Vol 36 (3) ◽  
pp. 132-139
Author(s):  
Edward Gilman ◽  
Chris Harchick ◽  
Maria Paz

Study was designed to evaluate impact of planting depth on root morphology inside nursery containers. Trees were planted shallow (13 mm) or deep (64 mm) into #3 Air-Pot™ containers, then shallow (0 mm) or deep (64 mm) into #15 containers prior to shifting them to their final #45 container size at the same depth. Trunk diameter (caliper) was significantly larger for both magnolia and maple planted shallow (13 mm) into #3, and then shallow into #15 containers when compared to planting deeper. However, differences were small and may not be relevant to a grower. No caliper or height differences among planting depths were found for elm. Presence of stem girdling roots in elm and magnolia growing in #45 containers increased with planting depth into # 3 containers. Downward re-orientation of main roots comprising the flare by #3 container wall, likely contributed to amount of roots growing over root flare. Maple root systems were not impacted by planting depth into #3 primarily due to adventitious root emergence from the buried portion of stem. Distance between substrate surface and top of root flare in finished #45 containers was not impacted by planting depth into #3 containers for any species. Planting elm and maple deeply into #15 led to more trunk-girdling by roots, a deeper root flare, and more roots growing over flare compared to planting shallow. Most root defects in all species were hidden from view because they were found below substrate surface. Presence of a visible root flare was not related to occurrence of root defects. Root balls on elm and maple were packed with roots which made it time consuming to remove substrate and roots above the root flare. Planting depth appears most crucial when shifting into #15 containers.


2021 ◽  
Vol 5 (1) ◽  
pp. 18-25
Author(s):  
Ari Wibowo

Roots represent the fundamental structure and material transport systems in plants. These important organs are used to study the adaptation processes in the environment. During the nursery stage, root evaluation serves as determining the seedling quality before transplant. This research aims to ascertain the root characteristics of several Arabica coffee varieties for a suitably vigorous root nursery system. Kaliwining Experimental Garden, Jember, Indonesia, served as the research location. The method used was a completely randomized design with a single factor, including arabica coffee varieties (Abesinia-3, Andungsari-1, BLP-4, Gayo-1, K-08, K-29, K-34, K-79, K-130, Kartika-1, Kayumas and Sigararutang). The result showed that the differences in the various sample factors significantly influenced the root properties. In addition, the entire observation parameters demonstrated a positive correlation except for the root and shoot ratio. Furthermore, BLP-4 v obtained the maximum values for plant height, stem diameter, fresh dry weight of taproot, and root biomass, while Gayo-1 variety observed the greatest number of leaves. Also, the Sigararutang variety indicated the highest value of the fresh and dry weight of shoot, fresh weight of lateral roots, and root length, while Kayumas and K-34 varieties acquired the lowest values of plant height, fresh and dry weight of shoot and root, as well as root length. Therefore, BLP-4, Gayo-1, and Sigararutang varieties exhibited the potentials of broader adaptability.


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