scholarly journals Effects of mycorrhizal inoculant, N:P supply ratio, and water depth on the growth and biomass allocation of three wetland plant species

2005 ◽  
Vol 83 (9) ◽  
pp. 1117-1125 ◽  
Author(s):  
Lauchlan H. Fraser ◽  
Larry M. Feinstein
Keyword(s):  
Biomass Allocation ◽  
Water Depth ◽  
Soil Surface ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Nutrient Supply ◽  
Wetland Plant ◽  
Below Ground ◽  
Root:Shoot Ratios ◽  
Water Depths

In the greenhouse, we investigated the growth and biomass allocation of three juvenile wetland species ( Carex tribuloides Wahl., Phalaris arundinacea L., and Rumex orbiculatus Gray) under three different water depths (–4, 0, and +2 cm relative to the soil surface), three N:P supply ratios (1:30, 1:1, 30:1), and two mycorrhizal inoculant treatments (arbuscular mycorrhizal (AM) fungi present, absent). After 6 weeks, the plants were harvested, separated to above- and below-ground parts, oven-dried, and weighed. The mycorrhizal inoculant significantly increased plant growth and reduced root:shoot ratios. At an N:P supply ratio of 30:1, plants with AM fungi had significantly greater biomass than those plants without AM fungi. However, at 1:1 N:P supply ratio, plants without AM fungi had greater biomass. Plants without AM fungi had higher root:shoot ratios at 0 and –4 cm water depth than plants with AM fungi. In general, C. tribuloides had the lowest growth, and unlike P. arundinacea and R. orbiculatus, was not affected by the water depth treatment. Growth of the wetland plants was limited more by nitrogen than by phosphorus. Our results suggest that at high N:P nutrient supply ratios and non-flooded conditions the growth of wetland seedlings can benefit by being inoculated with AM fungi.

Water-depth tolerances of the dominant emergent macrophytes of the Delta Marsh, Manitoba

1992 ◽  
Vol 70 (9) ◽  
pp. 1860-1867 ◽  
Author(s):  
Louisa Squires ◽  
A. G. Van der Valk
Keyword(s):  
Water Depth ◽  
Shoot Density ◽  
Shoot Length ◽  
Depth Gradient ◽  
Shoot Height ◽  
Delta Marsh ◽  
Below Ground ◽  
Seasonally Flooded ◽  
Water Depths

The growth (shoot height, cumulative shoot length, shoot density, above- and below-ground biomass) of seven emergent species growing at five different water depths was measured for 2 years. These species belonged to three different ecological classes: (i) upper marsh species (Carex atherodes, Scolochloa festucacea, and Phragmites australis) that occupy sections of the water-depth gradient that are only seasonally flooded in the Delta Marsh; (ii) lower marsh species (Typha glauca and Scirpus lacutris spp. glaucus) that occupy permanently flooded areas; and (iii) drawdown species (Scirpus lacustris spp. validus and Scirpus maritimus) that become established temporarily during drawdowns. Upper marsh species could not adjust their shoot length if they were growing in water deeper than 20 cm. Lower marsh species were able to do this in water up to 70 cm deep. All three types survived for 1 or 2 years in water too deep for long-term persistence. Scirpus species survived as tubers in areas with water too deep for them to grow. The distributions of the seven species in the experiments overlapped considerably, and all species cooccurred at water depths to 70 cm after 2 years of flooding. The predicted distribution in the four dominant species along a water-depth gradient were consistent with their actual distributions in the Delta Marsh, but their predicted distributions overlapped much more than is the case in the field. Key words: emergent vegetation, experiment, water-depth tolerance, plant growth, distributions.

scholarly journals Effects of pre- and post-transplant inoculation with commercial arbuscular mycorrhizal (AM) fungi on pelargonium (Pelargonium hortorum) and its microorganism community

2012 ◽  
Vol 21 (1) ◽  
pp. 52-61 ◽  
Author(s):  
Gergely Csima ◽  
Ildikó Hernádi ◽  
Katalin Posta
Keyword(s):  
Shoot Growth ◽  
Slow Release ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Nutrient Supply ◽  
Slow Release Fertilizer ◽  
Colony Forming Units ◽  
Diverse Community ◽  
Mycorrhizal Inoculation ◽  
Total Fungi

Rooted cuttings of geranium were grown with and without a slow release fertilizer and inoculated or not with a commercial inoculum containing AM fungi. After six weeks plants were transplanted into larger containers and one-half of the plants were inoculated with AM. Inoculation increased pelargonium growth along with nitrogen, phosphorous and potassium concentrations in shoot than caused a slight decrease in shoot growth and enhanced N concentration. Colony forming units of total fungi and bacteria in the rhizosphere were not influenced by AM;  although RFLP profiles of DNA isolated from bacteria living in rhizosphere showed a more diverse community in AM-inoculated than non-inoculated plants at low nutrient supply. Our results suggest that mycorrhizal inoculation not only has an effect on plant growth and uptake of elements but it also influences directly or indirectly the bacterial community of the rhizosphere.

Light and nutrient effects on growth and allocation of Inga vera (Leguminosae), a successional tree of Puerto Rico

2006 ◽  
Vol 36 (5) ◽  
pp. 1121-1128 ◽  
Author(s):  
Randall W Myster
Keyword(s):  
Biomass Allocation ◽  
Mycorrhizal Fungi ◽  
Light Availability ◽  
Arbuscular Mycorrhizal ◽  
Growth Responses ◽  
Light Levels ◽  
Nutrient Effects ◽  
Below Ground ◽  
High Degree ◽  
Growth And Allocation

With the aim of acquiring a better understanding of ecological growth and biomass allocation of Neo tropical trees, I inoculated Inga vera Willd. (Leguminosae) plants from cuttings with Rhizobium spp. and arbuscular mycorrhizal fungi and grew them in a greenhouse for 8 months under varying light (L), phosphorus (P), and nitrogen (N) treatments. I obtained the following results: (1) L, P × N, and L × P × N treatments affected every response variable, but most increases occurred under full light; that is, light levels influenced growth of Inga vera to a greater extent than did P and N additions by themselves; (2) response variables showed a high degree of similarity in regard to which combination of treatment levels had the greatest positive response (full light – low N – high P) and which combination led to other significant increases (full light – low N – no P, full light – high N – no P); and (3) percent AM colonization was affected mainly by light, not P levels. I conclude that growth responses of Inga vera are primarily controlled by light availability, which can interact with nutrient addition to affect biomass allocation to both above- and below-ground structures.

scholarly journals Tracking of Zinc Ferrite Nanoparticle Effects on Pea (Pisum sativum L.) Plant Growth, Pigments, Mineral Content and Arbuscular Mycorrhizal Colonization

Plants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 583
Author(s):  
Reda E. Abdelhameed ◽  
Nagwa I. Abu-Elsaad ◽  
Arafat Abdel Hamed Abdel Latef ◽  
Rabab A. Metwally
Keyword(s):  
Pisum Sativum ◽  
Plant Growth ◽  
Zinc Ferrite ◽  
Crop Improvement ◽  
Nanocrystalline Structure ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Transmission Electron ◽  
Agricultural Applications ◽  
The Impact

Important gaps in knowledge remain regarding the potential of nanoparticles (NPs) for plants, particularly the existence of helpful microorganisms, for instance, arbuscular mycorrhizal (AM) fungi present in the soil. Hence, more profound studies are required to distinguish the impact of NPs on plant growth inoculated with AM fungi and their role in NP uptake to develop smart nanotechnology implementations in crop improvement. Zinc ferrite (ZnFe2O4) NPs are prepared via the citrate technique and defined by X-ray diffraction (XRD) as well as transmission electron microscopy for several physical properties. The analysis of the XRD pattern confirmed the creation of a nanocrystalline structure with a crystallite size equal to 25.4 nm. The effects of ZnFe2O4 NP on AM fungi, growth and pigment content as well as nutrient uptake of pea (Pisum sativum) plants were assessed. ZnFe2O4 NP application caused a slight decrease in root colonization. However, its application showed an augmentation of 74.36% and 91.89% in AM pea plant shoots and roots’ fresh weights, respectively, compared to the control. Moreover, the synthesized ZnFe2O4 NP uptake by plant roots and their contents were enhanced by AM fungi. These findings suggest the safe use of ZnFe2O4 NPs in nano-agricultural applications for plant development with AM fungi.

scholarly journals Arbuscular mycorrhizal symbiosis facilitates apricot seedling (Prunus sibirica L.) growth and photosynthesis in northwest China

2021 ◽  
Author(s):  
Yinli Bi ◽  
Linlin Xie ◽  
Zhigang Wang ◽  
Kun Wang ◽  
Wenwen Liu ◽  
...  
Keyword(s):  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Northwest China ◽  
Shaanxi Province ◽  
Mycorrhizal Symbiosis ◽  
Seedling Physiology ◽  
Grassland Ecosystems ◽  
Time Period ◽  
Seedling Biomass ◽  
Experimental Treatments

AbstractArbuscular mycorrhizal (AM) fungi can successfully enhance photosynthesis (Pn) and plants growth in agricultural or grassland ecosystems. However, how the symbionts affect species restoration in sunlight-intensive areas remains largely unexplored. Therefore, this study’s objective was to assess the effect of AM fungi on apricot seedling physiology, within a specific time period, in northwest China. In 2010, an experimental field was established in Shaanxi Province, northwest China. The experimental treatments included two AM fungi inoculation levels (0 or 100 g of AM fungal inoculum per seedling), three shade levels (1900, 1100, and 550 µmol m−2 s−1), and three ages (1, 3, and 5 years) of transplantation. We examined growth, Pn, and morphological indicators of apricot (Prunus sibirica L.) seedling performances in 2011, 2013, and 2015. The colonization rate in mycorrhizal seedlings with similar amounts of shade is higher than the corresponding controls. The mycorrhizal seedling biomass is significantly higher than the corresponding non-mycorrhizal seedling biomass. Generally, Pn, stomatal conductance (Gs), transpiration rate (Tr), and water use efficiency are also significantly higher in the mycorrhizal seedlings. Moreover, mycorrhizal seedlings with light shade (LS) have the highest Pn. WUE is increased in non-mycorrhizal seedlings because of the reduction in Tr, while Tr is increased in mycorrhizal seedlings with shade. There is a significant increase in the N, P, and K fractions detected in roots compared with shoots. This means that LS had apparent benefits for mycorrhizal seedlings. Our results also indicate that AM fungi, combined with LS, exert a positive effect on apricot behavior.

scholarly journals Extraction of short chain chitooligosaccharides from fungal biomass and their use as promoters of arbuscular mycorrhizal symbiosis

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Andrea Crosino ◽  
Elisa Moscato ◽  
Marco Blangetti ◽  
Gennaro Carotenuto ◽  
Federica Spina ◽  
...  
Keyword(s):  
Fungal Biomass ◽  
Large Scale ◽  
Low Cost ◽  
Trichoderma Viride ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Production Costs ◽  
Short Chain ◽  
Mycorrhizal Symbiosis ◽  
Major Interest

AbstractShort chain chitooligosaccharides (COs) are chitin derivative molecules involved in plant-fungus signaling during arbuscular mycorrhizal (AM) interactions. In host plants, COs activate a symbiotic signalling pathway that regulates AM-related gene expression. Furthermore, exogenous CO application was shown to promote AM establishment, with a major interest for agricultural applications of AM fungi as biofertilizers. Currently, the main source of commercial COs is from the shrimp processing industry, but purification costs and environmental concerns limit the convenience of this approach. In an attempt to find a low cost and low impact alternative, this work aimed to isolate, characterize and test the bioactivity of COs from selected strains of phylogenetically distant filamentous fungi: Pleurotus ostreatus, Cunninghamella bertholletiae and Trichoderma viride. Our optimized protocol successfully isolated short chain COs from lyophilized fungal biomass. Fungal COs were more acetylated and displayed a higher biological activity compared to shrimp-derived COs, a feature that—alongside low production costs—opens promising perspectives for the large scale use of COs in agriculture.

scholarly journals Microplastic contamination of the drilling bivalve Hiatella arctica in Arctic rhodolith beds

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Sebastian Teichert ◽  
Martin G. J. Löder ◽  
Ines Pyko ◽  
Marlene Mordek ◽  
Christian Schulbert ◽  
...  
Keyword(s):  
Polyethylene Terephthalate ◽  
Water Depth ◽  
The Arctic ◽  
Polymer Composition ◽  
Bivalve Species ◽  
Filter Feeder ◽  
Hiatella Arctica ◽  
Long Term Consequences ◽  
Water Depths

AbstractThere is an increasing number of studies reporting microplastic (MP) contamination in the Arctic environment. We analysed MP abundance in samples from a marine Arctic ecosystem that has not been investigated in this context and that features a high biodiversity: hollow rhodoliths gouged by the bivalve Hiatella arctica. This bivalve is a filter feeder that potentially accumulates MPs and may therefore reflect MP contamination of the rhodolith ecosystem at northern Svalbard. Our analyses revealed that 100% of the examined specimens were contaminated with MP, ranging between one and 184 MP particles per bivalve in samples from two water depths. Polymer composition and abundance differed strongly between both water depths: samples from 40 m water depth showed a generally higher concentration of MPs and were clearly dominated by polystyrene, samples from 27 m water depth were more balanced in composition, mainly consisting of polyethylene, polyethylene terephthalate, and polypropylene. Long-term consequences of MP contamination in the investigated bivalve species and for the rhodolith bed ecosystem are yet unclear. However, the uptake of MPs may potentially impact H. arctica and consequently its functioning as ecosystem engineers in Arctic rhodolith beds.

scholarly journals Arbuscular Mycorrhizal Fungal Communities in the Soils of Desert Habitats

2021 ◽  
Vol 9 (2) ◽  
pp. 229
Author(s):  
Martti Vasar ◽  
John Davison ◽  
Siim-Kaarel Sepp ◽  
Maarja Öpik ◽  
Mari Moora ◽  
...  
Keyword(s):  
Plant Root ◽  
Significant Proportion ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Illumina Miseq ◽  
Desert Ecosystems ◽  
Fungal Dna ◽  
Root Symbionts ◽  
Arbuscular Mycorrhizal Fungal

Deserts cover a significant proportion of the Earth’s surface and continue to expand as a consequence of climate change. Mutualistic arbuscular mycorrhizal (AM) fungi are functionally important plant root symbionts, and may be particularly important in drought stressed systems such as deserts. Here we provide a first molecular characterization of the AM fungi occurring in several desert ecosystems worldwide. We sequenced AM fungal DNA from soil samples collected from deserts in six different regions of the globe using the primer pair WANDA-AML2 with Illumina MiSeq. We recorded altogether 50 AM fungal phylotypes. Glomeraceae was the most common family, while Claroideoglomeraceae, Diversisporaceae and Acaulosporaceae were represented with lower frequency and abundance. The most diverse site, with 35 virtual taxa (VT), was in the Israeli Negev desert. Sites representing harsh conditions yielded relatively few reads and low richness estimates, for example, a Saudi Arabian desert site where only three Diversispora VT were recorded. The AM fungal taxa recorded in the desert soils are mostly geographically and ecologically widespread. However, in four sites out of six, communities comprised more desert-affiliated taxa (according to the MaarjAM database) than expected at random. AM fungal VT present in samples were phylogenetically clustered compared with the global taxon pool, suggesting that nonrandom assembly processes, notably habitat filtering, may have shaped desert fungal assemblages.

scholarly journals LysM Receptor-Like Kinase LYK9 of Pisum Sativum L. May Regulate Plant Responses to Chitooligosaccharides Differing in Structure

2021 ◽  
Vol 22 (2) ◽  
pp. 711
Author(s):  
Irina V. Leppyanen ◽  
Olga A. Pavlova ◽  
Maria A. Vashurina ◽  
Andrey D. Bovin ◽  
Alexandra V. Dolgikh ◽  
...  
Keyword(s):  
Immune Response ◽  
Pisum Sativum ◽  
Binding Capacity ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Phytopathogenic Fungi ◽  
Plant Responses ◽  
Pisum Sativum L ◽  
Receptor Like Kinase ◽  
Pea Mutants

This study focused on the interactions of pea (Pisum sativum L.) plants with phytopathogenic and beneficial fungi. Here, we examined whether the lysin-motif (LysM) receptor-like kinase PsLYK9 is directly involved in the perception of long- and short-chain chitooligosaccharides (COs) released after hydrolysis of the cell walls of phytopathogenic fungi and identified in arbuscular mycorrhizal (AM) fungal exudates. The identification and analysis of pea mutants impaired in the lyk9 gene confirmed the involvement of PsLYK9 in symbiosis development with AM fungi. Additionally, PsLYK9 regulated the immune response and resistance to phytopathogenic fungi, suggesting its bifunctional role. The existence of co-receptors may provide explanations for the potential dual role of PsLYK9 in the regulation of interactions with pathogenic and AM fungi. Co-immunoprecipitation assay revealed that PsLYK9 and two proposed co-receptors, PsLYR4 and PsLYR3, can form complexes. Analysis of binding capacity showed that PsLYK9 and PsLYR4, synthesized as extracellular domains in insect cells, were able to bind the deacetylated (DA) oligomers CO5-DA–CO8-DA. Our results suggest that the receptor complex consisting of PsLYK9 and PsLYR4 can trigger a signal pathway that stimulates the immune response in peas. However, PsLYR3 seems not to be involved in the perception of CO4-5, as a possible co-receptor of PsLYK9.

scholarly journals Target of rapamycin, PvTOR, is a key regulator of arbuscule development during mycorrhizal symbiosis in Phaseolus

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Manoj-Kumar Arthikala ◽  
Kalpana Nanjareddy ◽  
Lourdes Blanco ◽  
Xóchitl Alvarado-Affantranger ◽  
Miguel Lara
Keyword(s):  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Target Of Rapamycin ◽  
Mycorrhizal Symbiosis ◽  
Energy Status ◽  
Symbiotic Associations ◽  
Expression Of Genes ◽  
Fungal Symbiosis ◽  
Am Fungus ◽  
Am Symbiosis

AbstractTarget of rapamycin (TOR) is a conserved central growth regulator in eukaryotes that has a key role in maintaining cellular nutrient and energy status. Arbuscular mycorrhizal (AM) fungi are mutualistic symbionts that assist the plant in increasing nutrient absorption from the rhizosphere. However, the role of legume TOR in AM fungal symbiosis development has not been investigated. In this study, we examined the function of legume TOR in the development and formation of AM fungal symbiosis. RNA-interference-mediated knockdown of TOR transcripts in common bean (Phaseolus vulgaris) hairy roots notably suppressed AM fungus-induced lateral root formation by altering the expression of root meristem regulatory genes, i.e., UPB1, RGFs, and sulfur assimilation and S-phase genes. Mycorrhized PvTOR-knockdown roots had significantly more extraradical hyphae and hyphopodia than the control (empty vector) roots. Strong promoter activity of PvTOR was observed at the site of hyphal penetration and colonization. Colonization along the root length was affected in mycorrhized PvTOR-knockdown roots and the arbuscules were stunted. Furthermore, the expression of genes induced by AM symbiosis such as SWEET1, VPY, VAMP713, and STR was repressed under mycorrhized conditions in PvTOR-knockdown roots. Based on these observations, we conclude that PvTOR is a key player in regulating arbuscule development during AM symbiosis in P. vulgaris. These results provide insight into legume TOR as a potential regulatory factor influencing the symbiotic associations of P. vulgaris and other legumes.

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