A technique for the study of floral development

1968 ◽  
Vol 46 (5) ◽  
pp. 720-722 ◽  
Author(s):  
Rolf Sattler
Keyword(s):  
Floral Development ◽  
Developmental Stages ◽  
Three Dimensional ◽  
Serial Sectioning ◽  
Direct Study ◽  
Floral Buds ◽  
Dimensional Picture

When floral buds are studied by serial sectioning, the obtained three-dimensional picture of the buds is a reconstruction which involves some theoretical elements. In contrast to this reconstructive method, the described technique permits the direct study of the three-dimensional developmental stages of flowers. Protoderm cells of floral apices and primordial appendages can be demonstrated.

Floral Development in Acacia pycnantha Benth. In Hook

1981 ◽  
Vol 29 (4) ◽  
pp. 385 ◽  
Author(s):  
MS Buttrose ◽  
WJR Grant ◽  
M Sedgley
Keyword(s):  
Light Microscopy ◽  
Pollen Development ◽  
Shoot Growth ◽  
Floral Development ◽  
Developmental Stages ◽  
Early Stage ◽  
Embryo Sac ◽  
Late Winter ◽  
Ovule Development ◽  
Floral Buds

Floral buds of Acacia pycnantha were produced in every month of the year on new shoot growth. The buds produced between November and May developed through to flowering but those produced between June and October aborted at an early stage. Differences in the rate of floral development caused buds produced several months apart to flower in the same month in late winter. Developmental stages from newly produced flower heads to anthesis were studied by light microscopy. Pollen development preceded ovule development and the 16-celled polyads were formed 1 month prior to flowering and before development of the embryo sac.

The cellular dynamics of pattern formation in the eye of Drosophila

Development ◽  
1985 ◽  
Vol 89 (1) ◽  
pp. 313-331
Author(s):  
Andrew Tomlinson
Keyword(s):  
Cell Division ◽  
Pattern Formation ◽  
Early Development ◽  
Developmental Stages ◽  
Computer Graphic ◽  
Three Dimensional ◽  
Dimensional Structure ◽  
Serial Sectioning ◽  
Electron Microscopic ◽  
Three Dimensional Structure

The establishment and early development of the ommatidial bundles in the presumptive eye of, Drosophila have been investigated using electron microscopic serial sectioning. A progression of developmental stages has been characterized. Initially simple, symmetrically constructed bundles develop into asymmetrical, more complex constructions. Computer graphic reconstruction has been used to show the three-dimensional structure of the various ommatidial bundles. Autoradiography has been used to locate the position of a region of cell division which is intimately associated with the formation of the ommatidial bundles.

Correlated Studies of Cellular Interactions Using TEM, Freeze Etching, and SEM

1974 ◽  
Vol 32 ◽  
pp. 320-321
Author(s):  
J. P. Revel
Keyword(s):  
Developmental Stages ◽  
Three Dimensional ◽  
Cell Movement ◽  
Cellular Interactions ◽  
Serial Sections ◽  
Cell Sheets ◽  
Freeze Etching ◽  
Early Developmental

Movement of individual cells or of cell sheets and complex patterns of folding play a prominent role in the early developmental stages of the embryo. Our understanding of these processes is based on three- dimensional reconstructions laboriously prepared from serial sections, and from autoradiographic and other studies. Many concepts have also evolved from extrapolation of investigations of cell movement carried out in vitro. The scanning electron microscope now allows us to examine some of these events in situ. It is possible to prepare dissections of embryos and even of tissues of adult animals which reveal existing relationships between various structures more readily than used to be possible vithout an SEM.

Dancing without Space – On Nijinsky'sL'Après-midi d'un Faune(1912)

2009 ◽  
Vol 27 (1) ◽  
pp. 28-64 ◽  
Author(s):  
Hanna Järvinen
Keyword(s):  
Three Dimensional ◽  
Two Dimensional ◽  
Theatrical Space ◽  
Principal Character ◽  
Vaslav Nijinsky ◽  
Important Shift ◽  
Dimensional Picture

Three-dimensional theatrical space is often taken for granted as a precondition of dance. Already in 1912, the choreographer Vaslav Nijinsky provoked much discussion with a work that seemingly turned the performance into a moving, two-dimensional picture. L'Après-midi d'un Faune has achieved notoriety because of the objections some contemporary critics raised against the ‘immoral’ behaviour of the principal character, but I argue the style of the work brought about an important shift in how dancing was conceptualised as something composed by a choreographic author.

Analyzing the Impact of X-Ray Tomography on the Reliability of Integrated Circuits

2015 ◽  
Author(s):  
Halit Dogan ◽  
Md Mahbub Alam ◽  
Navid Asadizanjani ◽  
Sina Shahbazmohamadi ◽  
Domenic Forte ◽  
...  
Keyword(s):  
Integrated Circuits ◽  
Integrated Circuit ◽  
3D Imaging ◽  
Three Dimensional ◽  
Serial Sectioning ◽  
Promising Technique ◽  
Flash Memories ◽  
X Ray ◽  
3D Information ◽  
The Impact

Abstract X-ray tomography is a promising technique that can provide micron level, internal structure, and three dimensional (3D) information of an integrated circuit (IC) component without the need for serial sectioning or decapsulation. This is especially useful for counterfeit IC detection as demonstrated by recent work. Although the components remain physically intact during tomography, the effect of radiation on the electrical functionality is not yet fully investigated. In this paper we analyze the impact of X-ray tomography on the reliability of ICs with different fabrication technologies. We perform a 3D imaging using an advanced X-ray machine on Intel flash memories, Macronix flash memories, Xilinx Spartan 3 and Spartan 6 FPGAs. Electrical functionalities are then tested in a systematic procedure after each round of tomography to estimate the impact of X-ray on Flash erase time, read margin, and program operation, and the frequencies of ring oscillators in the FPGAs. A major finding is that erase times for flash memories of older technology are significantly degraded when exposed to tomography, eventually resulting in failure. However, the flash and Xilinx FPGAs of newer technologies seem less sensitive to tomography, as only minor degradations are observed. Further, we did not identify permanent failures for any chips in the time needed to perform tomography for counterfeit detection (approximately 2 hours).

Combining Serial Sectioning, EBSD Analysis, and Image-Based Finite Element Modeling

MRS Bulletin ◽  
2008 ◽  
Vol 33 (6) ◽  
pp. 597-602 ◽  
Author(s):  
G. Spanos ◽  
D.J. Rowenhorst ◽  
A.C. Lewis ◽  
A.B. Geltmacher
Keyword(s):  
Finite Element ◽  
Finite Element Modeling ◽  
Electron Backscatter Diffraction ◽  
Three Dimensional ◽  
Research Area ◽  
Polycrystalline Materials ◽  
Serial Sectioning ◽  
3D Fem ◽  
Element Modeling ◽  
The Status

AbstractThis article first provides a brief review of the status of the subfield of three-dimensional (3D) materials analyses that combine serial sectioning, electron backscatter diffraction (EBSD), and finite element modeling (FEM) of materials microstructures, with emphasis on initial investigations and how they led to the current state of this research area. The discussions focus on studies of the mechanical properties of polycrystalline materials where 3D reconstructions of the microstructure—including crystallographic orientation information—are used as input into image-based 3D FEM simulations. The authors' recent work on a β-stabilized Ti alloy is utilized for specific examples to illustrate the capabilities of these experimental and modeling techniques, the challenges and the solutions associated with these methods, and the types of results and analyses that can be obtained by the close integration of experiments and simulations.

The Role of Three Dimension Computerized Imaging in Hand Surgery

1987 ◽  
Vol 12 (3) ◽  
pp. 349-352
Author(s):  
J. ENGEL ◽  
M. SALAI ◽  
B. YAFFE ◽  
R. TADMOR
Keyword(s):  
Three Dimensional ◽  
Hand Surgery ◽  
New Technique ◽  
Silicone Implants ◽  
Three Dimension ◽  
Two Dimensional ◽  
Radiological Imaging ◽  
Preliminary Results ◽  
Dimensional Picture

Three-dimensional computerized imaging is a new modality of radiological imaging. This new technique transforms the two-dimensional slices of bi-plane CT into a three-dimensional picture by a computer’s monitor adjusted to the system. This system enables the physician to rotate the angle of viewing of the desired region to any desired angle. Moreover, this system can delete certain features of different densities from the picture, such as silicone implants, thus improving visualization. Our preliminary results using this technique are presented. The advantages, pitfalls, and suggested future applications of this new technique in hand surgery are discussed.

Spatial- and temporal-restricted pattern for amelogenin gene expression during mouse molar tooth organogenesis

Development ◽  
1988 ◽  
Vol 104 (1) ◽  
pp. 77-85 ◽  
Author(s):  
M.L. Snead ◽  
W. Luo ◽  
E.C. Lau ◽  
H.C. Slavkin
Keyword(s):  
Gene Expression ◽  
In Situ Hybridization ◽  
Gene Transcription ◽  
Developmental Stages ◽  
Three Dimensional ◽  
Molar Tooth ◽  
Specific Expression ◽  
Amelogenin Gene ◽  
Stage Of Development

Position- and time-restricted amelogenin gene transcription was analysed in developing tooth organs using in situ hybridization with asymmetric complementary RNA probes produced from a cDNA specific to the mouse 26 × 10(3) Mr amelogenin. In situ analysis was performed on developmentally staged fetal and neonatal mouse mandibular first (M1) and maxillary first (M1) molar tooth organs using serial sections and three-dimensional reconstruction. Amelogenin mRNA was first detected in a cluster of ameloblasts along one cusp of the M1 molar at the newborn stage of development. In subsequent developmental stages, amelogenin transcripts were detected within foci of ameloblasts lining each of the five cusps comprising the molar crown form. The number of amelogenin transcripts appeared to be position-dependent, being more abundant on one cusp surface while reduced along the opposite surface. Amelogenin gene transcription was found to be bilaterally symmetric between the developing right and left M1 molars, and complementary between the M1 and M1 developing molars; indicating position-restricted gene expression resulting in organ stereoisomerism. The application of in situ hybridization to forming tooth organ geometry provides a novel strategy to define epithelial-mesenchymal signal(s) which are believed to be responsible for organ morphogenesis, as well as for temporal- and spatial-restricted tissue-specific expression of enamel extracellular matrix.

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