Acid phosphatase and protease release by the insectivorous plant Drosera rotundifolia

1977 ◽  
Vol 55 (4) ◽  
pp. 480-488 ◽  
Author(s):  
Finbarr G. Clancy ◽  
Michael D. Coffey
Keyword(s):  
Enzyme Activity ◽  
Acid Phosphatase ◽  
Hydrolytic Enzymes ◽  
Enzyme Release ◽  
Optimal Activity ◽  
Drosera Rotundifolia ◽  
Phosphorylated Compounds ◽  
Insectivorous Plant

The leaves of the insectivorous plant Drosera rotundifolia L. produced extracellular hydrolytic enzymes in response to feeding with gelatin. Enzyme release was first detected 1 to 2 days after feeding, reached a maximum on day 4, and then gradually declined. Optimal activity of both acid phosphatase (orthophosphoric-monoester phosphohydrolase (acid optimum), EC 3.1.3.2) and protease enzymes was in the acidic range of pH. The acid phosphatase attacked a range of phosphorylated compounds but its p-nitrophenylphosphatase (PNPPase) and ribonucleoside triphosphatase activities were highest. It displayed relatively low phosphomonoesterase (EC 3.1.3.1, 3.1.3.2) activity. Both acid phosphatase and protease enzymes were insensitive to the sulphydryl inhibitor N-ethylmaleimide. The acid phosphatase was strongly inhibited by fluoride and orthophosphate. The nature of the apparent induction of hydrolase enzyme activity is briefly discussed.

scholarly journals HYDROLYTIC ENZYMES DURING SPERMATOGENESIS IN RAT AN ELECTRON MICROSCOPIC AND HISTOCHEMICAL STUDY

1968 ◽  
Vol 16 (4) ◽  
pp. 249-262 ◽  
Author(s):  
ZOLTAN POSALAKI ◽  
DEZSÖ SZABÓ ◽  
ERNÖ BÁCSI ◽  
ISTVÁN ÖKRÖS
Keyword(s):  
Endoplasmic Reticulum ◽  
Enzyme Activity ◽  
Acid Phosphatase ◽  
Sertoli Cells ◽  
Hydrolytic Enzymes ◽  
Appreciable Amount ◽  
Second Phase ◽  
Nonspecific Esterase ◽  
Electron Microscopic ◽  
Two Phases

The localization of lipids and the activities of nonspecific esterase, aryl sulfatase and acid phosphatase were studied in different stages of spermatogenesis in rats. In addition, the distribution of acid phosphatase activity was demonstrated electron histochemically. The spermatogenetic cycle was divided into two phases—corresponding to the first and the last four stages of Roosen-Runge-Giesel (RG) classification. Spermatids in the first phase contained abundant endoplasmic reticulum with rosette formation and well developed Golgi apparatus with numerous vesicles. They displayed high activity of hydrolytic enzymes but contained no appreciable amount of lipids. The Sertoli cells contained large lipid granules but showed minimal enzyme activity. During the second phase reduction of the cytoplasm of spermatids with fragmentation of the endoplasmic reticulum and Golgi lamellae, accumulation of lipids, aggregation of ribonucleo-protein particles, formation of residual bodies and marked decrease of enzyme activity were seen. The Sertoli cells contained large mitochondria, well developed endoplasmic reticulum and numerous dense bodies and revealed high activities of hydrolytic enzymes and rapid depletion of lipids. These ultrastructural and histochemical findings suggested an interaction between the Sertoli cells and the developing spermatids which probably contributed to the regulation of spermatogenesis.

QUANTITATIVE STUDIES OF HYDROLYTIC ENZYME ACTIVITY IN THE SALIVARY GLAND OF CHIRONOMUS TENTANS (DIPTERA: CHIRONOMIDAE) DURING METAMORPHOSIS

1971 ◽  
Vol 103 (3) ◽  
pp. 454-457 ◽  
Author(s):  
Hans Laufer ◽  
Ki Ssu Schin
Keyword(s):  
Enzyme Activity ◽  
Salivary Gland ◽  
Acid Phosphatase ◽  
Protein Content ◽  
Lysosomal Enzymes ◽  
Specific Activity ◽  
Hydrolytic Enzyme ◽  
Hydrolytic Enzymes ◽  
Chironomus Tentans ◽  
Quantitative Studies

AbstractChanges in the hydrolytic enzymes, ribonuclease and acid phosphatase were investigated in the salivary gland of Chironomus tentans since these lysosomal enzymes may participate in hormonally stimulated tissue breakdown. Quantitative assays revealed 9- and 12-fold increases in the specific activity of these enzymes during pupation while the protein content of the gland was decreasing. These increases cannot be accounted for by decreased protein but may represent an activation, accumulation, or synthesis which seems to be important in gland breakdown at metamorphosis.

Smartphone Based Highly Sensitive Visualized Detection of Acid Phosphatase Enzyme Activity

2021 ◽  
Author(s):  
Rui Li ◽  
Yanan Sun ◽  
Lihua Jin ◽  
Xiaohong Qiao ◽  
Cong Li ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Acid Phosphatase ◽  
Point Of Care ◽  
Rapid Development ◽  
Practical Application ◽  
Fast Analysis ◽  
Highly Sensitive ◽  
Visualized Detection ◽  
Sensitive Method ◽  
Phosphatase Enzyme

With the rapid development of point-of-care (POC) technologies, the improvement of sensitive method featured with fast analysis and affordable devices has become an emerging requirement for the practical application. In...

scholarly journals Enzyme Histochemical Studies of Adipose Tissue in Porcine Yellow Fat Disease

1974 ◽  
Vol 11 (6) ◽  
pp. 465-476 ◽  
Author(s):  
L. H. J. C. Danse ◽  
W. A. Steenbergen-Botterweg
Keyword(s):  
Enzyme Activity ◽  
Adipose Tissue ◽  
Acid Phosphatase ◽  
Nonspecific Esterase ◽  
Cell Structures ◽  
Increased Activity

Adipose tissue of piglets with yellow fat disease had increased activity of nonspecific esterase, 5-nucleotidase, and acid phosphatase. Since these enzymes are associated with different cell structures and damage to these structures can result in increased enzyme activity, they are criteria for pathogenetic study of yellow fat disease.

An Investigation of the Histochemical Technique for the Localization of Acid Phosphomonoesterase

1950 ◽  
Vol s3-91 (15) ◽  
pp. 315-330
Author(s):  
FRANCES MACDONALD
Keyword(s):  
Enzyme Activity ◽  
Acid Phosphatase ◽  
Standard Technique ◽  
Formalin Fixation ◽  
Histochemical Technique ◽  
Acid Phosphomonoesterase

1. A method is described for assessing the depth of ‘staining’ obtained with the acid phosphatase technique and a detailed scheme is given of the standard technique used. 2. It is concluded that the technique specifically demonstrates the activity of acid phosphatase, since ‘staining’ is abolished in the absence of substrate, in heated sections, and in the presence of fluoride. 3. An investigation has been carried out to determine the extent to which the reaction is affected by altering various stages in the technique. 4. The effect of formalin fixation on the reaction has been investigated. 5. It has been shown that sites in the rabbit medulla having an affinity for the reagents used in the technique differ from the sites at which a precipitate is deposited as a result of enzyme activity. 6. Evidence is presented suggesting that the technique may not demonstrate the true physiological localization of the enzyme. 7. It is suggested that the technique may be of value as a neurohistological method.

SIGNIFICANCE OF TRANSAMINASE ACTIVITY OF SERUM

PEDIATRICS ◽  
1959 ◽  
Vol 24 (3) ◽  
pp. 360-361
Author(s):  
SAMUEL P. BESSMAN
Keyword(s):  
Alkaline Phosphatase ◽  
Enzyme Activity ◽  
Acid Phosphatase ◽  
Transaminase Activity ◽  
Specific Approach ◽  
Normal Tissues ◽  
The Past ◽  
Glycolytic Activity ◽  
Enzyme Characteristic ◽  
Phosphatase Alkaline

THE MEASUREMENT of enzyme activity of serum as an indicator of disease has a long history in medicine. In the past, it has been the aim of the designers of these methods to make them as specific as possible for assay of an enzyme characteristic of a particular system or group of similar organs. Examples of these venerable tests are those for amylase, acid phosphatase, alkaline phosphatase and choline esterase in the serum. Warburg made the first departure from this specificity by demonstrating that the activity of triosephosphate dehydrogenase in the serum of animals with cancer was much greater than that of controls. This test was partially specific, for as Warburg had earlier shown, the glycolytic activity of tumors is much greater than that of normal tissues. The non-specific approach became extreme with the introduction of the measurement of the glutamic-oxalacetic transaminase reaction in the diagnosis of acute coronary disease.

Acid phosphatase, alkaline phosphatase, and nitrate reductase activity of selected ectomycorrhizal fungi

1989 ◽  
Vol 67 (3) ◽  
pp. 750-753 ◽  
Author(s):  
Iwan Ho
Keyword(s):  
Alkaline Phosphatase ◽  
Enzyme Activity ◽  
Acid Phosphatase ◽  
Nitrate Reductase ◽  
Phosphatase Activity ◽  
Ectomycorrhizal Fungi ◽  
Acid Phosphatase Activity ◽  
Nitrate Reductase Activity ◽  
Reductase Activity ◽  
Phosphatase Alkaline

Seventeen isolates, encompassing five genera and eight species of ectomycorrhizal fungi, were compared for acid phosphatase, alkaline phosphatase, and nitrate reductase activity. Isolates within species differed in enzyme activity and isozyme patterns by host specificity and site (as exemplified by the genus Suillus). Host and site may have affected phosphatase enzyme activity. Generally, the Douglas-fir associates, which dominate in mesic sites, have higher acid phosphatase activity than pine associates, which mostly occupy xeric sites; however, pine associates from mesic sites also have higher acid phosphatase activity (e.g., S. tomentosus). In four isolates of Amanita muscaria, the effect of site was also apparent. Two of them, which have significantly higher acid phosphatase activity than the others, were isolated from mesic sites. The isozyme pattern of the genus Suillus appeared to be separated by host groups. Other isolates with only one species also differed more or less by host groups. They shared at least one band within host groups, except for the two isolates of Paxillus involutus from different hosts. The P. involutus S-403 isolated from an orchard showed much higher nitrate reductase activity than all other isolates. No apparent differences in nitrate reductase activity were found between the other isolates.

scholarly journals Soil enzyme activity in the dry valley phytocenoses under the influence of Acer negundo L.

2021 ◽  
Vol 31 ◽  
pp. 00029
Author(s):  
Oksana Tsandekova
Keyword(s):  
Enzyme Activity ◽  
Hydrolytic Enzymes ◽  
Soil Condition ◽  
Invertase Activity ◽  
Horizontal Differentiation ◽  
Natural Ecosystems ◽  
Crown Density ◽  
Dry Valley ◽  
Medium Activity ◽  
And Control

The activity of hydrolytic enzymes in the soil of dry valley phytocenoses under the influence of ash-leaved maple was investigated. The research objects were selected taking into account the ranking of plantations by crown density. Soil samples were collected depending on the horizontal differentiation of communities in the undercrown and outer zones of phytogenic fields. An increase in the enzyme activity during the period of active tree growth among experimental and control samples was established. Among the enzymes, invertase demonstrated the highest activity, while protease and phosphatase were characterised by medium activity. An increased invertase activity was found in the trees with a high crown density as compared to the trees of other groups. The obtained data can be used as diagnostic indicators of soil condition for monitoring natural ecosystems.

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