Déterminisme de la reproduction sexuée du Phaeosphaeria (Leptosphaeria) nodorum agent de la septoriose du blé. II. Action de la température et de la lumière

Perithecial formation of Phaeosphaeria nodorum is obtained in vitro on sterilized wheat straws, under strict conditions of light and temperature. The absence of any reproductive form in the dark indicates photoinduction. The different parameters of light (duration, quality, and intensity) influence perithecial morphogenesis. A 12-h photoperiod, near ultraviolet light (300 nm < λ < 400 nm), and intensities of 400 and 600 μW/cm2 are the most favourable conditions for perithecial differentiation. The perithecial production occurs best at 10 °C, is markedly reduced at 14 °C, and absent above 14 °C. Light and temperature have a sequential influence on the successive stages of perithecial development. Whereas primordial formation is photoinhibited and cryostimulated, transformation to the early stage of perithecial development is photoinduced and not affected by temperatures of 10 and 18 °C. The subsequent stages up to ascogenesis are photostimulated and cryoinduced, and ascosporogenesis is photoindependent and cryostimulated. Key words: Phaeosphaeria, Leptosphaeria nodorum, perithecia, light, temperature.

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Déterminisme de la reproduction sexuée de Phaeosphaeria (Leptosphaeria) nodorum, agent de la septoriose du blé. I. Hétérothallisme et rôle des microspores

Canadian Journal of Botany ◽

10.1139/b91-013 ◽

1991 ◽

Vol 69 (1) ◽

pp. 95-99 ◽

Cited By ~ 13

Author(s):

P. Halama ◽

L. Lacoste

Keyword(s):

Key Words ◽

Leptosphaeria Nodorum ◽

Phaeosphaeria Nodorum ◽

The One

Recently identified conditions for in vitro perithecia formation made it possible to demonstrate the existence of previously suggested heterothallism in Phaeosphaeria nodorum. Of the 28 possible pairwise crosses between the eight cultures derived from the spores of a single ascus, 9 were fertile, whereas none of the cultures ever produced fertile perithecia on their own. This result is smaller than the one obtained in classical bipolar heterothallism and suggests the existence of another incompatibility mechanism. The growth and the colour of mycelia derived from eight single ascospores from the same ascus seem to cosegregate with the factor for heterothallism. The formation of microspores in colonies from single ascospores indicates that they may play a role in the formation of perithecia. Used for fertilization, these microspores showed that they were able to bring the complementary nucleus, thus enabling the differentiation of perithecia. However, they are able to germinate and do not behave strictly as spermatia. Key words: Phaeosphaeria, Leptosphaeria nodorum, heterothallism, microspores.

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Les organes de reproduction de l'ascomycète Phaeosphaeria (Leptosphaeria) nodorum et de sa forme asexuée Septoria nodorum

Canadian Journal of Botany ◽

10.1139/b92-176 ◽

1992 ◽

Vol 70 (7) ◽

pp. 1401-1408 ◽

Cited By ~ 4

Author(s):

P. Halama ◽

A. Parguey-Leduc ◽

L. Lacoste

Keyword(s):

Key Words ◽

Reproductive Organs ◽

The Other ◽

Stagonospora Nodorum ◽

Septoria Nodorum ◽

Asexual Stage ◽

Leptosphaeria Nodorum ◽

Phaeosphaeria Nodorum

The reproductive organs of one of the pathogens responsible for the septoria diseases of wheat were studied from cultures. There are two types of pycnidia for the asexual stage (one belongs to Septoria nodorum Berk. (=Stagonospora nodorum (Berk.) Castellani & Germano); the other is microconidial) and pseudothecia for the teleomorph: Phaeosphaeria nodorum (E. Müller) Hedjaroude (=Leptosphaeria nodorum E. Müller). The latter are differentiated from glomerular primordia, and their development is typical of an ascolocular pyrenomycete, with a perilocular layer generating descending pseudoparaphyses and periphyses lining the ostiolar cavity. The asci, with a "Jack-in-the-box" dehiscence, are bitunicate. Key words: Phaeosphaeria nodorum, Leptosphaeria, ontogeny and structure, pseudothecia, pycnidia.

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In vitro Propagation and Ex vitro Rooting of Blueberry Plantlets

Plant Tissue Culture and Biotechnology ◽

10.3329/ptcb.v18i2.3650 ◽

1970 ◽

Vol 18 (2) ◽

pp. 187-195 ◽

Cited By ~ 2

Author(s):

Zhao Guang-jie ◽

Wang Zhan-bin ◽

Wang Dan

Keyword(s):

Tissue Culture ◽

Key Words ◽

Plant Tissue ◽

In Vitro Propagation ◽

Root Formation ◽

Early Stage ◽

In Vitro Regeneration ◽

Basal Medium ◽

Ex Vitro

Effects of different concentrations of 2-ip and IBA in WPM basal medium for Blomidon blueberry in vitro propagation and four different rooting agents at the early stage after transplantation showed that 15 mg/l of 2-ip is the best concentration to induce shoots. For optimum in vitro root formation 10 µM IBA was found to be best and four rooting agents for seedling transplantation according to their effects were No.2>, No.4>, No.3 >, water > and No. 1. Key words: Blomidon, Tissue culture, In vitro regeneration, Rooting agent D.O.I. 10.3329/ptcb.v18i2.3650 Plant Tissue Cult. & Biotech. 18(1): 187-195, 2008 (December)

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One-Pot Synthesis of Homogeneous EuF3 Nanoplates: A Near-Ultraviolet Light-Induced Red-Emitting Bifunctional Platform for in vitro Cell Imaging and Solid-State Lighting

ChemistrySelect ◽

10.1002/slct.201803982 ◽

2019 ◽

Vol 4 (8) ◽

pp. 2275-2280

Author(s):

Peng Du ◽

Ying Liu ◽

Yeon-Ju Kim ◽

Jae Su Yu

Keyword(s):

Solid State ◽

Ultraviolet Light ◽

Cell Imaging ◽

Solid State Lighting ◽

One Pot ◽

One Pot Synthesis ◽

Near Ultraviolet

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Photochemical interaction of dictamnine, a furoquinoline alkaloid, with fungal DNA in vitro and in vivo

Canadian Journal of Microbiology ◽

10.1139/m82-071 ◽

1982 ◽

Vol 28 (5) ◽

pp. 468-473 ◽

Cited By ~ 11

Author(s):

Gaby E. Pfyffer ◽

G. H. Neil Towers

Keyword(s):

Ultraviolet Light ◽

Penicillium Italicum ◽

Mucor Hiemalis ◽

Near Ultraviolet ◽

Long Wave ◽

Cellular Target ◽

Calf Thymus ◽

Fungal Dna

The furoquinoline alkaloid dictamnine has been shown to provoke lethal damage to filamentous fungi in near ultraviolet light. The phototoxicity was more pronounced against Mucor hiemalis and Mucor ramannianus than against Fusarium graminearum and Penicillium italicum. In vitro, labeled dictamnine was shown to form covalent monoadducts with purified DNA from M. hiemalis in the presence of long-wave ultraviolet light. Addition of [3H]dictamnine to cultures of the same organism showed photobinding with the fungal DNA in vivo. These results support the suggestion made earlier, on the basis of in vitro experiments with calf thymus DNA, that DNA represents a major cellular target in vivo for the phototoxicity of the alkaloid.

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Fibroblast Interaction with Different Abutment Surfaces: In Vitro Study

International Journal of Molecular Sciences ◽

10.3390/ijms21061919 ◽

2020 ◽

Vol 21 (6) ◽

pp. 1919 ◽

Cited By ~ 1

Author(s):

Luigi Canullo ◽

Tullio Genova ◽

Esperanza Gross Trujillo ◽

Guillermo Pradies ◽

Sara Petrillo ◽

...

Keyword(s):

Ultraviolet Light ◽

Early Stage ◽

Argon Plasma ◽

In Vitro Study ◽

Side Surface ◽

Cellular Adhesion ◽

Grade 5 ◽

Tissue Healing ◽

Titanium Surfaces

Background: Attaining an effective mucosal attachment to the transmucosal part of the implant could protect the peri-implant bone. Aim: To evaluate if chair side surface treatments (plasma of Argon and ultraviolet light) may affect fibroblast adhesion on different titanium surfaces designed for soft tissue healing. Methods: Grade 5 titanium discs with four different surface topographies were subdivided into 3 groups: argon-plasma; ultraviolet light, and no treatment. Cell morphology and adhesion tests were performed at 20 min, 24 h, and 72 h. Results: Qualitative observation of the surfaces performed at the SEM was in accordance with the anticipated features. Roughness values ranged from smooth (MAC Sa = 0.2) to very rough (XA Sa = 21). At 20 min, all the untreated surfaces presented hemispherical cells with reduced filopodia, while the cells on treated samples were more spread with broad lamellipodia. However, these differences in spreading behavior disappeared at 24 h and 72 h. Argon-plasma, but not UV, significantly increased the number of fibroblasts independently of the surface type but only at 20 min. Statistically, there was no surface in combination with a treatment that favored a greater cellular adhesion. Conclusions: Data showed potential biological benefits of treating implant abutment surfaces with the plasma of argon in relation to early-stage cell adhesion.

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Ultrastructural Changes in Three Different Mammalian Cells Following Ultraviolet Laser Irradiation

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100095017 ◽

1972 ◽

Vol 30 ◽

pp. 350-351

Author(s):

K. Shankar Narayan ◽

Kailash C. Gupta ◽

Tohru Okigaki

Keyword(s):

Ultraviolet Light ◽

Mammalian Cells ◽

Biological Effects ◽

Survival Rates ◽

Chinese Hamster ◽

Cell Types ◽

Differential Sensitivity ◽

Ultrastructural Changes ◽

Ultraviolet Laser

The biological effects of short-wave ultraviolet light has generally been described in terms of changes in cell growth or survival rates and production of chromosomal aberrations. Ultrastructural changes following exposure of cells to ultraviolet light, particularly at 265 nm, have not been reported.We have developed a means of irradiating populations of cells grown in vitro to a monochromatic ultraviolet laser beam at a wavelength of 265 nm based on the method of Johnson. The cell types studies were: i) WI-38, a human diploid fibroblast; ii) CMP, a human adenocarcinoma cell line; and iii) Don C-II, a Chinese hamster fibroblast cell strain. The cells were exposed either in situ or in suspension to the ultraviolet laser (UVL) beam. Irradiated cell populations were studied either "immediately" or following growth for 1-8 days after irradiation.Differential sensitivity, as measured by survival rates were observed in the three cell types studied. Pattern of ultrastructural changes were also different in the three cell types.

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Holistic Metabolomic Laboratory-Developed Test (LDT): Development and Use for the Diagnosis of Early-Stage Parkinson’s Disease

Metabolites ◽

10.3390/metabo11010014 ◽

2020 ◽

Vol 11 (1) ◽

pp. 14

Author(s):

Petr G. Lokhov ◽

Dmitry L. Maslov ◽

Steven Lichtenberg ◽

Oxana P. Trifonova ◽

Elena E. Balashova

Keyword(s):

Parkinson’S Disease ◽

Molecular Weight ◽

Parkinson's Disease ◽

High Resolution Mass Spectrometry ◽

Early Stage ◽

Disease Diagnosis ◽

The Body ◽

Low Molecular Weight ◽

Low Molecular Weight Compounds

A laboratory-developed test (LDT) is a type of in vitro diagnostic test that is developed and used within a single laboratory. The holistic metabolomic LDT integrating the currently available data on human metabolic pathways, changes in the concentrations of low-molecular-weight compounds in the human blood during diseases and other conditions, and their prevalent location in the body was developed. That is, the LDT uses all of the accumulated metabolic data relevant for disease diagnosis and high-resolution mass spectrometry with data processing by in-house software. In this study, the LDT was applied to diagnose early-stage Parkinson’s disease (PD), which currently lacks available laboratory tests. The use of the LDT for blood plasma samples confirmed its ability for such diagnostics with 73% accuracy. The diagnosis was based on relevant data, such as the detection of overrepresented metabolite sets associated with PD and other neurodegenerative diseases. Additionally, the ability of the LDT to detect normal composition of low-molecular-weight compounds in blood was demonstrated, thus providing a definition of healthy at the molecular level. This LDT approach as a screening tool can be used for the further widespread testing for other diseases, since ‘omics’ tests, to which the metabolomic LDT belongs, cover a variety of them.

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