Influence of treatment with quercetin on lipid parameters and oxidative stress of pregnant diabetic rats

2013 ◽  
Vol 91 (2) ◽  
pp. 171-177 ◽  
Author(s):  
Camila Pereira Braga ◽  
Ana Carolina Momentti ◽  
Fernando Barbosa Peixoto ◽  
Rafaela de Fátima Ferreira Baptista ◽  
Felipe André dos Santos ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Biochemical Parameters ◽  
Wistar Rats ◽  
Diabetic Rats ◽  
Lipid Hydroperoxides ◽  
Female Wistar Rats ◽  
Lipid Parameters ◽  
And Oxidative Stress ◽  
Pregnant Diabetic

Among the numerous coadjuvant therapies that could influence the incidence and progression of diabetic complications, antioxidants and flavonoids are currently being tested in clinical trials. We investigated the effect of quercetin on biochemical parameters in streptozotocin-induced (60 mg/kg body mass, by intraperitoneal injection) diabetic rats. A total of 32 female Wistar rats were distributed among 4 groups as follows: control (G1); control treated with quercetin (G2); diabetic (G3); and diabetic treated with quercetin (G4). Quercetin administered to pregnant diabetic rats controlled dyslipidemia and improved lipid profiles in diabetes mellitus, regulated oxidative stress by reducing the generation of lipid hydroperoxides, and increased the activity of the antioxidant enzyme glutathione peroxidase.

scholarly journals Comparative Study of the Antioxidant Effects of Metformin, Glibenclamide, and Repaglinide in Alloxan-Induced Diabetic Rats

2016 ◽  
Vol 2016 ◽  
pp. 1-5 ◽  
Author(s):  
Bonaventure Chukwunonso Obi ◽  
Theophine Chinwuba Okoye ◽  
Victor Eshu Okpashi ◽  
Christiana Nonye Igwe ◽  
Edwin Olisah Alumanah
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Diabetic Complications ◽  
Significant Proportion ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Control Group ◽  
Diabetic Control Group ◽  
Antioxidant Effects ◽  
And Oxidative Stress

Diabetes mellitus is one of the serious global health problems affecting a significant proportion of both developed and developing countries. Overproduction of free radicals and oxidative stress has been associated with the development of diabetic complications. In the present study, the antioxidant effects of metformin (MET), glibenclamide (GLI), and repaglinide (REP) were evaluated in alloxan-induced diabetic rats. The findings from this study may possibly help in understanding the efficacy of these standard drugs in managing the complications arising from diabetes mellitus (DM). Alloxan (130 mg/kg BW) was administered as a single dose to induce diabetes. Four (4) groups of rats (n=6) were used; group 1 served as diabetic control while groups 2, 3, and 4 were the diabetic test groups that received MET (25 mg/kg), GLI (2.5 mg/kg), and REP (0.5 mg/kg), respectively. The result of the study showed significant (p<0.05) improvement in the altered antioxidant enzymes (SOD, CAT) and GSH concentration in diabetic treated rats compared with the diabetic control group. MET and REP produced significant effect on the MDA concentration while GLI showed insignificant reduction in the MDA concentration compared with the diabetic control. Findings from this study suggest that the administration of MET, GLI, and REP exerts significant antioxidant effects in alloxan-induced diabetic rats, thus contributing to the protective effect against oxidative stress-induced damage during diabetic complications.

scholarly journals Beneficial effects of walnut (Juglans regia L.) oil-derived polyunsaturated fatty acid prevents a prooxidant status and hyperlipidemia in pregnant rats with diabetes

2020 ◽  
Author(s):  
Bingmei Sun ◽  
Hua Yan ◽  
Chao Li ◽  
Linlin Yin ◽  
Fei Li ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Fatty Acid ◽  
Gestational Diabetes ◽  
Gestational Diabetes Mellitus ◽  
Juglans Regia ◽  
Diabetic Rats ◽  
Pregnant Rats ◽  
Beneficial Effects ◽  
Pregnant Diabetic

Abstract Background: Gestational diabetes mellitus has a long-term effect on pregnant women. Walnut (Juglans regia L.) oil-derived polyunsaturated fatty acid (PUFA) possesses multifarious pharmacological activities. This study investigated the beneficial effects of walnut oil-derived PUFA on glucose metabolism, pregnancy outcomes, oxidative stress, and lipid metabolism in gestational diabetes mellitus.Methods: The GDM rat model was generated by intraperitoneal injection of streptozotocin (40 mg/kg) on gestational day (GD) 6, GD7 and GD8. The differences between groups were estimated using one-way ANOVA followed by the Tukey’s multiple comparison test for post-hoc analysis.Results: The results indicated that PUFA could mitigate GDM in pregnant diabetic rats, as embodied by the decrease of fasting blood glucose and the increase of plasma insulin and hepatic glycogen levels. Also, PUFA could suppress oxidative stress in pregnant diabetic rats, as reflected by the decrease of malondialdehyde (MDA) content, an increase of superoxide dismutase (SOD), catalase (CAT) and gutathione peroxidase (GSH-Px) activities. PUFA could also mitigate the abnormal changes of lipid profiles in plasma and hepatic tissue. Moreover, the relative mRNA expression of sterol regulatory element-binding transcription factor-1 (SREBP-1), stearoyl-CoA desaturase-1 (SCD-1), fatty acid synthase (FAS), and acetyl-coenzyme A carboxylase (ACC), was suppressed by PUFA in pregnant diabetic rats.Conclusions: These results suggested that PUFA supplementation during pregnancy is beneficial in preventing diabetic complications in pregnant rats.

scholarly journals Curcumin and Its Analog Alleviate Diabetes-induced Damages by Regulating Inflammation and Oxidative Stress in Brain of Diabetic Rats​

2020 ◽  
Author(s):  
Chengfeng Miao ◽  
Hanbin Chen ◽  
Yulian Li ◽  
Ying Guo ◽  
Feifei Xu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Western Blot ◽  
Diabetic Rats ◽  
High Dose ◽  
Diabetic Encephalopathy ◽  
Sprague Dawley ◽  
The Brain ◽  
And Oxidative Stress ◽  
Myelin Staining

Abstract Background: Diabetic encephalopathy is a severe diabetes complication with cognitive dysfunction and neuropsychiatric disability. The mechanisms underlying diabetic encephalopathy is believed to be relevant with oxidative stress, vascular amylin deposition, immune receptors, inflammation, etc. This study wanted to evaluate the ability of curcumin and its analog A13 to alleviate oxidative stress and inflammation in diabetes-induced damages in brain.Methods: Sixty adult male Sprague-Dawley rats were divided into 5 groups: normal control (NC) group, diabetes mellitus (DM) group, curcumin-treated diabetes mellitus (CUR) group, high dose of A13-treated diabetes mellitus (HA) group, low dose of A13-treated diabetes mellitus (LA) group. Activation of the nuclear factor kappa-B (NF-κB p65) pathway was detected by RT-qPCR, immunohistochemical (IHC) staining and Western blot; oxidative stress was detected by biochemical detection kit; brain tissue sections were stained with hematoxylin–eosin (HE) staining and Myelin staining. Results: RT-qPCR, IHC staining and Western blot showed that curcumin and A13 treatment could inhibit the NF-κB p65 pathway. Curcumin and A13 increased the activity of superoxide dismutase and decreased the malondialdehyde level in the brain of diabetic rats. Furthermore, HE staining and Myelin staining demonstrated that the histological lesions of the brain in diabetic rats could be significantly ameliorated by curcumin and A13.Conclusion: Curcumin analog A13 could alleviate the damages in the brain of diabetes rats by regulating the pathways of inflammation and oxidative stress. A13 may be a new potential therapeutic agent for diabetic encephalopathy.

scholarly journals Status of Antioxidants Molecules and Lipid Peroxidation in the Diabetic Testes

2018 ◽  
Vol 26 (1) ◽  
Author(s):  
Kishwor Bhandari ◽  
Sanju Acharya ◽  
AK Srivastava
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Lipid Peroxidation ◽  
Molecular Study ◽  
Epidemiological Studies ◽  
Diabetic Rats ◽  
Enzymatic Antioxidants ◽  
Chronic Hyperglycemia ◽  
Diabetic Model ◽  
And Oxidative Stress

Introduction: According to the epidemiological studies, diabetes mellitus has become a potential cause of male infertility. Knowledge regarding how diabetes mellitus interferes with the process of spermatogenesis and results in infertility needs the molecular study in the testis in diabetic condition. Enhanced oxidative stress and changes in antioxidant capacity are considered to play an important role in the pathogenesis of chronic diabetes mellitus. So, this study was established to investigate the activity of enzymatic antioxidants and oxidative stress in the testis of diabetic model rats. Material & Methods: Diabetes mellitus was induced in the rat by intraperitoneal injection of Streptozotocin. The rats were sacrificed and the dissection was done to take out the testis. The testes were processed for the activity of enzymatic antioxidants. Results: It was found that oxidative stress was increased in the testes of diabetic rats. The sperms were also affected by the chronic hyperglycemia.

scholarly journals Curcumin Supplementation Decreases Intestinal Adiposity Accumulation, Serum Cholesterol Alterations, and Oxidative Stress in Ovariectomized Rats

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Maurilio da Silva Morrone ◽  
Carlos Eduardo Schnorr ◽  
Guilherme Antônio Behr ◽  
Juciano Gasparotto ◽  
Rafael Calixto Bortolin ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Wistar Rats ◽  
Serum Levels ◽  
Antioxidant Defenses ◽  
Ovariectomized Rats ◽  
Oral Supplementation ◽  
Stress Markers ◽  
Female Wistar Rats ◽  
And Oxidative Stress ◽  
Hormone Deprivation

The aim of this study was to investigate the potential of curcumin oral supplementation (50 and 100 mg/Kg/day, for 30 days) in circumventing menopause-associated oxidative stress and lipid profile dysfunctions in a rat ovariectomy (OVX) model. Female Wistar rats were operated and randomly divided into either sham-operated or OVX groups. Sham-operated group (n=8) and one OVX group (n=11) were treated with vehicle (refined olive oil), and the other two OVX groups received curcumin at 50 or 100 mg/Kg/day doses (n=8/group). OVX vehicle-treated animals presented a higher deposition of intestinal adipose tissue as well as increased serum levels of IL-6, LDL, and total cholesterol when compared to sham-operated rats. In addition, several oxidative stress markers in serum, blood, and liver (such as TBARS, carbonyl, reduced-sulphydryl, and nonenzymatic antioxidant defenses) were altered toward a prooxidant status by OVX. Interestingly, curcumin supplementation attenuated most of these parameters to sham comparable values. Thus, the herein presented results show that curcumin may be useful to ameliorate lipid metabolism alterations and oxidative damage associated with hormone deprivation in menopause.

Effects of the ingestion of different kinds of white grape juice (Vitis labrusca) during adolescence on body weight, biochemical parameters and oxidative stress in liver of adult Wistar rats

2019 ◽  
Vol 291 ◽  
pp. 110-116 ◽  
Author(s):  
Elenara Simoni Kovaleski ◽  
Luciana Kneib Gonçalves ◽  
Gabrielli Bortolato ◽  
Jessica Pereira Marinho ◽  
Luiz Fernando Lopes Silva ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Body Weight ◽  
Biochemical Parameters ◽  
Wistar Rats ◽  
Grape Juice ◽  
Vitis Labrusca ◽  
And Oxidative Stress

scholarly journals Curcumin and its analog alleviate diabetes-induced damages by regulating inflammation and oxidative stress in brain of diabetic rats

2021 ◽  
Vol 13 (1) ◽  
Author(s):  
Chengfeng Miao ◽  
Hanbin Chen ◽  
Yulian Li ◽  
Ying Guo ◽  
Feifei Xu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Western Blot ◽  
Diabetic Rats ◽  
High Dose ◽  
Diabetic Encephalopathy ◽  
Sprague Dawley ◽  
The Brain ◽  
And Oxidative Stress ◽  
Myelin Staining

Abstract Background Diabetic encephalopathy is a severe diabetes complication with cognitive dysfunction and neuropsychiatric disability. The mechanisms underlying diabetic encephalopathy is believed to be relevant with oxidative stress, vascular amylin deposition, immune receptors, inflammation, etc. This study wanted to evaluate the ability of curcumin and its analog A13 to alleviate oxidative stress and inflammation in diabetes-induced damages in brain. Methods Sixty adult male Sprague–Dawley rats were divided into 5 groups: normal control (NC) group, diabetes mellitus (DM) group, curcumin-treated diabetes mellitus (CUR) group, high dose of A13-treated diabetes mellitus (HA) group, low dose of A13-treated diabetes mellitus (LA) group. Activation of the nuclear factor kappa-B (NF-κB p65) pathway was detected by RT-qPCR, immunohistochemical (IHC) staining and Western blot; oxidative stress was detected by biochemical detection kit; brain tissue sections were stained with hematoxylin–eosin (HE) staining and Myelin staining. Results RT-qPCR, IHC staining and Western blot showed that curcumin and A13 treatment could inhibit the NF-κB p65 pathway. Curcumin and A13 increased the activity of superoxide dismutase and decreased the malondialdehyde level in the brain of diabetic rats. Furthermore, HE staining and Myelin staining demonstrated that the histological lesions of the brain in diabetic rats could be significantly ameliorated by curcumin and A13. Conclusion Curcumin analog A13 could alleviate the damages in the brain of diabetes rats by regulating the pathways of inflammation and oxidative stress. A13 may be a new potential therapeutic agent for diabetic encephalopathy.

scholarly journals Effect of moderate exercise on renal changes and oxidative stress in ovariectomized rats with type 1 diabetes mellitus

2019 ◽  
Vol 6 (13) ◽  
pp. 331-345 ◽  
Author(s):  
Mayane Oliveira Rebouças da Silveira ◽  
Liliany Souza de Brito Amaral ◽  
Samira Itana de Souza ◽  
Halanna Rocha Ferraz ◽  
Jéssica Alves Dias ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Type 1 Diabetes ◽  
Renal Function ◽  
Aerobic Exercise ◽  
Type 1 Diabetes Mellitus ◽  
Diabetic Rats ◽  
Ovariectomized Rats ◽  
And Oxidative Stress

This study evaluated the aerobic exercise effects of moderate and progressive intensity on renal function and structure, and oxidative stress in ovariectomized rats with type 1 diabetes mellitus induced by streptozotocin (STZ). Eighteen Wistar rats were divided into 3 groups: OSC - ovariectomized and sedentary control rats; OSD - ovariectomized and sedentary diabetic rats; and OTD - ovariectomized and trained diabetic rats. After induction of diabetes, the OTD group was submitted to eight weeks of exercise. Twenty-four hours after the last training session urine samples were collected. Blood samples and kidneys were collected after euthanasia for renal function analysis, histology, morphometry and oxidative stress. Our results have shown a reduction of the weight gain, increase of kidney weight and postprandial glycemia in diabetic rats. However, exercise decreased glycosuria and prevented the proteinuria in OTD group rats. Focal segmental glomerulosclerosis (FSGS), juxtamedullary glomerular tuft area, tubulointerstitial lesions (TIL), brush border loss and tubular cell debridement were reduced in OTD rats. In addition, exercise training decreased urinary and plasma concentrations of thiobarbituric acid reactive substance (TBARS). Our results demonstrate the beneficial effect of progressive aerobic exercise on proteinuria, glycosuria, and renal structure in ovariectomized diabetic rats, which may be mediated in part by reduction of oxidative stress.

Sign in / Sign up

Export Citation Format

Share Document