THE BACTERIAL FLORA OF LOW-ACID VEGETABLES CANNED AT 212° F.: I. A PRELIMINARY STUDY OF THE EFFECTS OF VARIOUS PROCESSING PROCEDURES

1945 ◽  
Vol 23f (4) ◽  
pp. 231-238 ◽  
Author(s):  
E. H. Garrard ◽  
J. H. L. Truscott ◽  
J. W. Conner
Keyword(s):  
Processing Time ◽  
Bacterial Flora ◽  
Anaerobic Bacteria ◽  
Water Bath ◽  
Tomato Juice ◽  
Snap Beans ◽  
Preliminary Study ◽  
Aerobic And Anaerobic

Low-acid vegetables were processed in a water-bath at 212° F. for one-half to three hours continuously and also intermittently, as in home canning. Peas, corn, and snap beans processed by these methods showed the presence of many types of surviving aerobic and anaerobic bacteria, but none showed spoilage when containers were effectively sealed. The same was found true for commercially canned products. The addition of 50% tomato juice to snap beans considerably reduced the number of bacterial survivors and made possible the greatest number of sterile containers, even with processing time of one and one-half hours.

scholarly journals Microbiological, histological, technological, and toxicological preliminary study of dog's canned food

2018 ◽  
Vol 49 (2) ◽  
pp. 110
Author(s):  
S. RANTOS (Σ. ΡΑΝΤΟΣ) ◽  
L. PANTOULAS (Λ. ΠΑΝΤΟΥΛΑΣ) ◽  
I. SARAKATSIANOS (Ι. ΣΑΡΑΚΑΤΣΙΑΝΟΣ) ◽  
G. ROZOS (Γ. ΡΟΖΟΣ) ◽  
N. PAPAIOANNOU (Ν. ΠAΠΑΪΩΑNNOΥ)
Keyword(s):  
Connective Tissue ◽  
Water Activity ◽  
Anaerobic Bacteria ◽  
Full Agreement ◽  
Canned Food ◽  
Lead And Cadmium ◽  
Preliminary Study ◽  
Soya Proteins ◽  
Aerobic And Anaerobic

During this study canned food for dogs of six firms were investigated (42 samples totally). Microbiologically the following parameters were checked: the commercial sterility, the water activity (aw), the pH, the presence of aerobic and anaerobic bacteria after incubation of tins in 25 ° C for 28 days, 32 ° C for 21 days and 55 ' C for 8 days. Histologically, the existence of different kinds of tissues was examined. Technologically Weende's analysis and separation of the tin's content were made. Toxicologically, the concentration of lead and cadmium were countered. The results showed that dog's canned food, in Greece, are supersterillised. Muscular, adipose and connective tissue are used for their production as well as byproducts and soya proteins by some firms. The results of Weende's analysis are in full agreement with the amount of nutriments that they were written on tins' labels.

Immunoglobulin- and complement-coated bacteria in pus from peritonsillar abscesses

1998 ◽  
Vol 112 (7) ◽  
pp. 634-638 ◽  
Author(s):  
Markus Lilja ◽  
Simo Räisänen ◽  
Lars-Eric Stenfors
Keyword(s):  
Bacterial Species ◽  
Bacterial Flora ◽  
Anaerobic Bacteria ◽  
Inflammatory Cells ◽  
Immunofluorescence Assay ◽  
Peritonsillar Abscess ◽  
Secretory Iga ◽  
Intracellular Bacteria ◽  
Protective Mechanisms ◽  
Aerobic And Anaerobic

AbstractFifty-five samples of pus were collected from 51 acute, non-perforated, two spontaneously ruptured and two recurrent peritonsillar abscesses (35 males and 18 females; median age 18 years) and analysed regarding (i) aerobic and anaerobic bacteria (standard culturing), (ii) morphology of bacteria and inflammatory cells (direct microscopy of acridine orange-stained material), and (iii) the percentage of bacteria coated with immunoglobulins IgG, secretory IgA (SIgA) andIgM and complement cleavage product C3b (immunofluorescence assay). Seventy-one per cent of the abscesses harboured a mixed bacterial flora of various aerobes and anaerobes. In none of the cases with a single bacterial species (27 per cent) could immunoglobulin- or complement-coated bacteria be found. In abscesses with a mixed flora, 18 per cent harboured IgG-coated, 15 per cent SIgA-coated, five per cent IgM-coated and five per cent C3b-coated bacteria, respectively. All pus samples contained inflammatory cells in abundance but they were mostly deformed and only occasionally could intracellular bacteria be recognized. Insufficient immunoglobulin-coating of bacteria might be an important aetiopathogenic factor in the development of a peritonsillar abscess. Bactericide in the abscesses is accomplished chiefly by protective mechanisms not dependent on antigen recognition by antibodies.

Bacterial Flora of "Normal" Sinuses

PEDIATRICS ◽  
1990 ◽  
Vol 86 (4) ◽  
pp. 649-649
Author(s):  
Itzhak Brook
Keyword(s):  
Maxillary Sinus ◽  
Bacterial Flora ◽  
Anaerobic Bacteria ◽  
Oral Flora ◽  
Sinus Cavity ◽  
Aerobic And Anaerobic ◽  
Maxillary Sinuses

To the Editor.— I read with interest the study by Arruda et al1 concerning the recovery of organisms from maxillary sinuses. The authors were able to isolate bacteria from 6 (29%) of the 21 patients with normal maxillary sinus radiographs. These findings support our earlier observation in which we recovered aerobic and anaerobic bacteria in uninfected maxillary sinuses.2 The colonization of the sinus cavity with oral flora may explain the chain of events that lead to infection.

THE BACTERIAL FLORA OF LOW-ACID VEGETABLES CANNED AT 212° F.: II. A PRELIMINARY STUDY OF THE EFFECTS OF EXTRACTS FROM PROCESSED TOMATO JUICE AND FROM TOMATO PLANTS ON BACTERIA INVOLVED IN FOOD PRESERVATION

1946 ◽  
Vol 24f (6) ◽  
pp. 467-473
Author(s):  
J. W. Conner
Keyword(s):  
Tomato Fruit ◽  
Bacterial Flora ◽  
Antibacterial Properties ◽  
Food Preservation ◽  
Tomato Juice ◽  
Tomato Plants ◽  
Food Industries ◽  
Tomato Seedlings ◽  
Test Organisms ◽  
Preliminary Study

Commercially processed tomato juice, dehydrated tomato stems, leaves, seed, and fruit (variety, Sutton's Very Earliest), and tomato seedlings (variety Pan America), were extracted with methanol and the extracts tested for antibacterial properties against certain species of bacteria important in the food industries, and other Gram-positive and Gram-negative types. The extracts prepared from tomato juice and tomato fruit inhibited the growth of most of the test organisms.

CHANGES IN SMALL INTESTINE MICROFLORA IN PATIENTS WITH ACUTE INTESTINAL OBSTRUCTION

2020 ◽  
pp. 16-18
Author(s):  
V. M. Lykhman ◽  
O. M. Shevchenko ◽  
Ye. O. Bilodid ◽  
Igor Vladimirovich Volchenko ◽  
I. A. Kulyk ◽  
...  
Keyword(s):  
Small Intestine ◽  
Gastrointestinal Tract ◽  
Species Composition ◽  
Intestinal Obstruction ◽  
Bacterial Flora ◽  
Anaerobic Bacteria ◽  
Acute Intestinal Obstruction ◽  
Gram Positive ◽  
Mechanical Intestinal Obstruction ◽  
Facultative Anaerobic Bacteria

Among urgent surgical diseases of abdominal cavity, an acute intestinal obstruction is the most difficult to be diagnosed and treated. Leading factor, determining the development of pathophysiological processes is considered to be the progressive manifestations of enteric insufficiency syndrome, resulting in intestinal barrier impairment, negative changes in ecology of intestinal flora, increased endotoxins. To identify the small intestine microflora in acute intestinal obstruction and determine the role of dysbiotic disorders in clinical manifestations of main pathological process, a study was conducted in 60 patients with mechanical intestinal obstruction. The small intestine has a relatively rare microflora, consisting mainly of gram−positive facultative aerobic microorganisms, streptococci, lactobacilli. The distal ileum in nearly 30−55 % of healthy people contains scanty microflora, and yet the flora of this area differs from the microbial population of the higher gastrointestinal tract due to higher concentration of gram−negative bacteria. Optional−anaerobic coliform bacilli, anaerobic bifidobacteria and fusobacteria, bacteroids, the number of which starts exceeding the one of gram−positive species, are presented in significant quantities. Distal to the ileocecal valve there are significant changes in the microflora quantitative and species composition. Obligatory anaerobic bacteria become the predominant part of microflora, exceeding the number of aerobic and facultative anaerobic bacteria. The bacterial flora in different parts of gastrointestinal tract has its own specifics and is quite constant, as a result of the interaction of many factors, regulating the bacterial population in small intestine. The most important among them are: acidity of gastric juice, normal peristaltic activity of the intestine, bacterial interactions and immune mechanisms. Disorders of the intestine motor and evacuation function with its obstruction lead to slow passage of the chyme and contamination of the upper gastrointestinal tract with new types of microbes. There is a syndrome of small intestine excessive colonization, which means an increased concentration of bacterial populations in it, similar in species composition to the colon microflora. Pathological intra−intestinal contents become a source of endogenous infection and re−infection of the patient, leads to internal digestive disorders, which is manifested by syndrome of malabsorption of proteins, carbohydrates and vitamins. Key words: acute intestinal obstruction, small intestinal microflora, conditionally pathogenic microorganisms, intestinal biocenosis.

Normal vaginal aerobic and anaerobic bacterial flora of the rhesus macaque ( Macacca mulatta )

1991 ◽  
Vol 20 (8) ◽  
pp. 409-413 ◽  
Author(s):  
Laurie Doyle ◽  
Cynthia L. Young ◽  
Spencer S. Jang ◽  
Sharon L. Hillier
Keyword(s):  
Rhesus Macaque ◽  
Bacterial Flora ◽  
Aerobic And Anaerobic

Aerobic and Anaerobic Bacteria in the Middle ear and ear Canal in Acute Otitis Media

1982 ◽  
Vol 93 (sup386) ◽  
pp. 100-102 ◽  
Author(s):  
J. Luotonen ◽  
A. M. M. Jokipii ◽  
P. Sipilä ◽  
J. Väyrynen ◽  
L. Jokipii ◽  
...  
Keyword(s):  
Otitis Media ◽  
Middle Ear ◽  
Anaerobic Bacteria ◽  
Acute Otitis Media ◽  
Ear Canal ◽  
Aerobic And Anaerobic

Decontamination of the Oral Cavity. Effect of Six Local Anti-Microbial Preparations in Comparison to Water and Parafilm as Controls

1988 ◽  
Vol 16 (2) ◽  
pp. 98-106 ◽  
Author(s):  
C. Matula ◽  
M. Hildebrandt ◽  
G. Nahler
Keyword(s):  
Hydrogen Peroxide ◽  
Oral Cavity ◽  
Anaerobic Bacteria ◽  
Crossover Design ◽  
Double Blind ◽  
Bacterial Counts ◽  
Aerobic And Anaerobic ◽  
Cavity Effect ◽  
Bacterial Effect ◽  
Observation Period

Six preparations (four liquid and two solid) were tested in a double-blind crossover design for their anti-bacterial effect on aerobic and anaerobic bacteria in saliva of 12 volunteers. The four liquid preparations contained either tyrothricin, hexetidine, hydrogen peroxide or ethanol and were tested against a rinse with water as control. The two solid preparations, in the form of lozenges, contained tyrothricin in doses of 4 or 10 mg and were tested against parafilm. A single rinse with either tyrothricin or hexetidine resulted in a significant reduction of aerobic and anaerobic bacteria in saliva which lasted for the whole 1h observation period and was considerably more effective than hydrogen peroxide or ethanol. Similar results were seen with the solid preparations. The lozenge containing 10 mg tyrothricin was the most effective and was the only formulation capable of reducing oral aerobic bacterial counts by a factor of approximately 100.

scholarly journals In Vitro and In Vivo Antibacterial Activities of a Novel Glycylcycline, the 9-t-Butylglycylamido Derivative of Minocycline (GAR-936)

1999 ◽  
Vol 43 (4) ◽  
pp. 738-744 ◽  
Author(s):  
P. J. Petersen ◽  
N. V. Jacobus ◽  
W. J. Weiss ◽  
P. E. Sum ◽  
R. T. Testa
Keyword(s):  
Anaerobic Bacteria ◽  
Tetracycline Resistance ◽  
Protective Effects ◽  
Gram Negative ◽  
E Coli ◽  
Resistance Determinants ◽  
Aerobic And Anaerobic ◽  
Ribosomal Protection

ABSTRACT The 9-t-butylglycylamido derivative of minocycline (TBG-MINO) is a recently synthesized member of a novel group of antibiotics, the glycylcyclines. This new derivative, like the first glycylcyclines, theN,N-dimethylglycylamido derivative of minocycline and 6-demethyl-6-deoxytetracycline, possesses activity against bacterial isolates containing the two major determinants responsible for tetracycline resistance: ribosomal protection and active efflux. The in vitro activities of TBG-MINO and the comparative agents were evaluated against strains with characterized tetracycline resistance as well as a spectrum of recent clinical aerobic and anaerobic gram-positive and gram-negative bacteria. TBG-MINO, with an MIC range of 0.25 to 0.5 μg/ml, showed good activity against strains expressing tet(M) (ribosomal protection), tet(A), tet(B),tet(C), tet(D), and tet(K) (efflux resistance determinants). TBG-MINO exhibited similar activity against methicillin-resistant Staphylococcus aureus (MRSA), penicillin-resistant streptococci, and vancomycin-resistant enterococci (MICs at which 90% of strains are inhibited, ≤0.5 μg/ml). TBG-MINO exhibited activity against a wide diversity of gram-negative aerobic and anaerobic bacteria, most of which were less susceptible to tetracycline and minocycline. The in vivo protective effects of TBG-MINO were examined against acute lethal infections in mice caused by Escherichia coli, S. aureus, andStreptococcus pneumoniae isolates. TBG-MINO, administered intravenously, demonstrated efficacy against infections caused byS. aureus including MRSA strains and strains containingtet(K) or tet(M) resistance determinants (median effective doses [ED50s], 0.79 to 2.3 mg/kg of body weight). TBG-MINO demonstrated efficacy against infections caused by tetracycline-sensitive E. coli strains as well asE. coli strains containing either tet(M) or the efflux determinant tet(A), tet(B), ortet(C) (ED50s, 1.5 to 3.5 mg/kg). Overall, TBG-MINO shows antibacterial activity against a wide spectrum of gram-positive and gram-negative aerobic and anaerobic bacteria including strains resistant to other chemotherapeutic agents. The in vivo protective effects, especially against infections caused by resistant bacteria, corresponded with the in vitro activity of TBG-MINO.

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