scholarly journals Karyotype structure and cytogenetic markers of Amoimyrmex bruchi and Amoimyrmex silvestrii: the contribution to understanding leafcutting ant relationships

Genome ◽  
2021 ◽  
Author(s):  
Ricardo Micolino ◽  
Brenda Carla de Lima Baldez ◽  
Andrés Fernando F. Sánchez Restrepo ◽  
Luis Calcaterra ◽  
Maykon Passos Cristiano ◽  
...  
Keyword(s):  
Molecular Cytogenetics ◽  
Chromosome Morphology ◽  
Integrative Approach ◽  
Social Parasite ◽  
Leafcutter Ants ◽  
Sister Clade ◽  
Karyotype Structure ◽  
Terrestrial Environments ◽  
The Many ◽  
Leafcutting Ants

Leafcutting ants are considered the most important herbivores in terrestrial environments throughout the Neotropics. <i>Amoimyrmex</i> is the sister clade of the remaining leafcutter ants from the genera <i>Atta</i> and <i>Acromyrmex</i>. <i>Amoimyrmex striatus</i> was the only species cytogenetically studied within the genus and shares the same chromosomal number with <i>Atta</i>, bearing 22 chromosomes, whereas <i>Acromyrmex</i> bears 38 chromosomes, with the exception of the social parasite <i>Acromyrmex ameliae</i> (2n=36). Our objective here was to analyze cytogenetically the species of <i>Amoimyrmex bruchi</i> and <i>Amoimyrmex silvestrii</i>, as well as to describe the karyotype of these sister species, by means of an integrative approach using classical and molecular cytogenetics. We aimed to characterize cytogenetic markers that contribute to the systematics and taxonomy of the genus. Our results showed that the karyotypes of these two species are very similar, with an identical chromosome number (2n=22), chromosome morphology (2K=20m+2sm), and location of 18S rDNA and the telomeric repeat TTAGG on the chromosomes. Yet, the microsatellite probe GA(15) showed variation across the species and populations studied. We suggest that both species diverged relatively recently and are unmistakably sisters because of the many shared characteristics, including the highly conserved karyotypes.

An Integrative Approach to the Assessment and Treatment of Inflammatory Conditions

2017 ◽  
Author(s):  
Mark Liponis ◽  
Bettina Martin
Keyword(s):  
Integrative Approach ◽  
The Past ◽  
Inflammatory Conditions ◽  
Improved Outcomes ◽  
Targeted Treatments ◽  
Great Progress ◽  
Assessment And Treatment ◽  
The Many ◽  
Made In

The past two decades have seen great progress in recognizing the importance of inflammation in medicine. Increased focus on inflammation in both prevention and treatment has improved outcomes and quality of life in chronic diseases. Science has improved our understanding of inflammation’s many causes and effects on health, and many advances have been made in the availability of targeted therapeutic options for treating inflammation. This chapter gives an overview of recognizing the many causes of inflammation, its many targeted treatments strategies, and the questions that still surround it. It discusses several integrative approaches to reducing inflammation, including exercise, diet, and different strategies for managing sleep, mood, and stress, such as meditation and massage.

scholarly journals Molecular cytogenetics of Hedysarum gmelinii Ledeb. and H. setigerum Turcz. (Fabaceae) from populations of Southern Siberia

2021 ◽  
Vol 20 (1) ◽  
pp. 517-519
Author(s):  
O. Yu. Yurkevich ◽  
T. E. Samatadze ◽  
I. Yu. Selyutina ◽  
S. A. Zoshchuk ◽  
A. V. Amosova ◽  
...  
Keyword(s):  
Chromosome Numbers ◽  
Related Species ◽  
Karyotype Analysis ◽  
Molecular Cytogenetics ◽  
5S Rdna ◽  
Chromosome Morphology ◽  
Closely Related Species ◽  
Southern Siberia ◽  
Close Relationship ◽  
First Time

For the first time, a comparative karyotype analysis of closely related species Hedysarum gmelinii andH. setigerum (Hedysarum section Multicaulia) grown in Southern Siberia, has been performed by molecular cytogeneticmarkers. Chromosome numbers in karyotypes of these species were specified – 2n = 4х = 32. In some accessions, additionalB chromosomes were revealed. FISH analyses indicated high similarities in chromosome morphology and also patternsof chromosomal distributions of 45S and 5S rDNA clusters in karyotypes of H. gmelinii and H. setigerum, which confirmsthe close relationship between their genomes.

scholarly journals Cytogenetic data for sixteen ant species from North-eastern Amazonia with phylogenetic insights into three subfamilies

2020 ◽  
Vol 14 (1) ◽  
pp. 43-60
Author(s):  
Hilton Jeferson Alves Cardoso de Aguiar ◽  
Luísa Antônia Campos Barros ◽  
Linda Inês Silveira ◽  
Frédéric Petitclerc ◽  
Sandrine Etienne ◽  
...  
Keyword(s):  
Terrestrial Ecosystems ◽  
Molecular Data ◽  
South American ◽  
Atta Sexdens ◽  
Leafcutter Ants ◽  
Cytogenetic Data ◽  
North Eastern ◽  
Karyotype Structure ◽  
Morphological And Molecular Data ◽  
First Time

Ants play essential roles in most terrestrial ecosystems and may be considered pests for agriculture and agroforestry. Recent morphological and molecular data have challenged conventional ant phylogeny and the interpretation of karyotypic variations. Existing Neotropical ant cytogenetic data focus on Atlantic rainforest species, and provide evolutionary and taxonomic insight. However, there are data for only 18 Amazonian species. In this study, we describe the karyotypes of 16 ant species belonging to 12 genera and three subfamilies, collected in the Brazilian state of Amapá, and in French Guiana. The karyotypes of six species are described for the first time, including that of the South American genus Allomerus Mayr, 1878. The karyotype of Crematogaster Lund, 1831 is also described for the first time for the New World. For other species, extant data for geographically distinct populations was compared with our own data, e.g. for the leafcutter ants Acromyrmex balzani (Emery, 1890) and Atta sexdens (Linnaeus, 1758). The information obtained for the karyotype of Dolichoderus imitator Emery, 1894 differs from extant data from the Atlantic forest, thereby highlighting the importance of population cytogenetic approaches. This study also emphasizes the need for good chromosome preparations for studying karyotype structure.

scholarly journals Description of Longidorus bordonensis sp. nov. from Portugal, with systematics and molecular phylogeny of the genus (Nematoda, Longidoridae)

2020 ◽  
Vol 96 (1) ◽  
pp. 175-193
Author(s):  
Carlos Gutiérrez-Gutiérrez ◽  
Margarida Teixeira Santos ◽  
Maria Lurdes Inácio ◽  
Jonathan D. Eisenback ◽  
Manuel Mota
Keyword(s):  
Phylogenetic Trees ◽  
Cork Oak ◽  
Morphological Data ◽  
Rrna Genes ◽  
Integrative Approach ◽  
Sister Clade ◽  
Molecular Phylogenetic ◽  
Wild Olive ◽  
First Time ◽  
Short Body

The genus Longidorus currently comprises 176 species of polyphagous plant ectoparasites, including eight species that vector nepoviruses. Longidorus is one of the most difficult genera to accurately identify species because of the similar morphology and overlapping measurements and ratios among species. Sequences of ribosomal RNA (rRNA)-genes are a powerful level-species diagnostic tool for the genus Longidorus. From 2015 to 2019, a nematode survey was conducted in vineyards and agro-forest environments in Portugal. The populations of Longidorus spp. were characterized through an integrative approach based on morphological data and molecular phylogenetic analysis from rRNA genes (D2-D3 expansion segments of the 28S, ITS1, and partial 18S), including the topotype of L. vinearum. Longidorus bordonensissp. nov., a didelphic species recovered from the rhizosphere of grasses, is described and illustrated. Longidorus vineacola, with cork oak and wild olive as hosts, is also characterized. This is the first time that L. wicuolea, from cork oak, is reported for Portugal. Bayesian inference (BI) phylogenetic trees for these three molecular markers established phylogenetic relationships among the new species with other Longidorus spp. Phylogenetic trees indicated that i) L. bordonensissp. nov. is clustered together with other Longidorus spp. and forms a sister clade with L. pini and L. carpetanensis, sharing a short body and odontostyle length, and elongate to conical female tail, and ii) all the other species described and illustrated are phylogenetically associated, including the topotype isolate of L. vinearum.

scholarly journals Filling the gap: a new ant-butterfly symbiosis in the forest canopy

2021 ◽  
Author(s):  
Gabriela Pérez-Lachaud ◽  
Franklin H. Rocha ◽  
Carmen Pozo ◽  
Lucas A. Kaminski ◽  
Noemy Seraphim ◽  
...  
Keyword(s):  
Forest Canopy ◽  
Tissue Sample ◽  
Integrative Approach ◽  
Social Parasite ◽  
Symbiotic Relationships ◽  
The Core ◽  
Ponerine Ant ◽  
Evolutionary Step ◽  
Site Dependence ◽  
Colony Integration

Abstract Myrmecophilous butterflies can establish complex symbiotic relationships with ants. An elusive symbiotic caterpillar wandering among the brood of the aggressive ponerine ant, Neoponera villosa, was found inside the core of a nest built in the myrmecophytic bromeliad Aechmea bracteata. This is the first caterpillar found in symbiosis with a species of the subfamily Ponerinae. Tissue sample was barcoded, and an integrative approach was used to identify it as Pseudonymphidia agave, a poorly known Pachythonina member in the riodinid myrmecophilous tribe Nymphidiini. The caterpillar has a general tank-like body morphology, without projections or tubercles, and is covered dorsally by specialized flat setae that form an armor of small plates. Ant-organs potentially related to the caterpillar-ant signaling, such as perforated cupola organs and tentacle nectary organs, are present. These functional traits, together with the stable social integration, suggest that P. agave is a specialized social parasite of N. villosa. However, several knowledge gaps remain, notably the oviposition site, dependence on bromeliad association, steps to colony integration, and the larval diet through ontogeny. Carnivory has been reported in all known members of the Pachythonina subtribe (caterpillars prey on honeydew-producing hemipterans), and a shift to myrmecophagy inside the ant nests is a possible evolutionary step.

Chromosomal characterization in native populations of Elymus scabrifolius from Argentina through classical and molecular cytogenetics (FISH–GISH)

Genome ◽  
2012 ◽  
Vol 55 (8) ◽  
pp. 591-598 ◽  
Author(s):  
P.A. Tomas ◽  
G.E. González ◽  
G.E. Schrauf ◽  
L. Poggio
Keyword(s):  
In Situ Hybridization ◽  
Chromosome Pair ◽  
Molecular Cytogenetics ◽  
Chromosome Morphology ◽  
Genomic In Situ Hybridization ◽  
Dapi Staining ◽  
Accurate Identification ◽  
Native Populations ◽  
Strong Hybridization

The karyotype of Elymus scabrifolius (Döll) J.H. Hunz. (2n = 4x = 28) was investigated by DAPI staining and in situ hybridization. All the accessions studied presented a symmetric and uniform karyotype constituted by 9m+2m–sm+3sm. DAPI stain showed 1–7 conspicuous bands in all the chromosomes and polymorphisms between accessions. FISH experiments carried out with 45S rDNA as probe (pTa71) showed strong hybridization signals on the metacentric SAT-chromosome pair 8; the submetacentric SAT-chromosome pair 13 presented weaker hybridization. FISH using pSc119.2 clone as probe identified five chromosome pairs. Then, the combination of chromosome morphology, DAPI-staining, and FISH enabled the accurate identification of each chromosome pair in E. scabrifolius. Genomic in situ hybridization (GISH) experiments using Hordeum DNA as probe on mitotic metaphases confirmed unequivocally the presence of the H genome in E. scabrifolius, allowing us to observe six uniformly labeled chromosome pairs and two chromosome pairs with only one arm labeled. The remaining six chromosome pairs were weakly labeled. The rehybridization of FISH slides with Hordeum DNA as probe allow us to assign the genomic provenance of most of the chromosomes in the studied accessions. Moreover, intergenomic rearrangement was detected between genome H and the still unknown progenitor genome.

scholarly journals Cytogenetic characterization, rDNA mapping and quantification of the nuclear DNA content in Seriolella violacea Guichenot, 1848 (Perciformes, Centrolophidae)

2020 ◽  
Vol 14 (3) ◽  
pp. 319-328
Author(s):  
Cristian Araya-Jaime ◽  
Claudio Palma-Rojas ◽  
Elisabeth Von Brand ◽  
Alfonso Silva
Keyword(s):  
Dna Content ◽  
Nuclear Dna ◽  
Molecular Cytogenetics ◽  
Fish Fauna ◽  
Nuclear Dna Content ◽  
5S Rdna ◽  
Chromosomal Evolution ◽  
Rdna Sequences ◽  
Rdna Sites ◽  
Karyotype Structure

Seriolella violacea Guichenot, 1848 is an important component of the fish fauna of the Chilean coast and is of great economic interest. Cytogenetic information for the family Centrolophidae is lacking and the genomic size of five of the twenty-eight species described for this family are is barely known. This study aimed to describe for the first time the karyotype structure via classical and molecular cytogenetics analysis with the goal of identifying the constitutive heterochromatin distribution, chromosome organization of rDNA sequences and quantification of nuclear DNA content. The karyotype of S. violacea is composed of 48 chromosomes, with the presence of conspicuous blocks of heterochromatin on chromosomal pairs one and two. FISH assay with a 5S rDNA probe, revealed the presence of fluorescent markings on the heterochromatic block of pair one. The 18S rDNA sites are located exclusively on pair two, characterizing this pair as the carrier of the NOR. Finally, the genomic size of S. violacea was estimated at 0.59 pg of DNA as C-value. This work represents the first effort to document the karyotype structure and physical organization of the rDNA sequences in the Seriolella genome, contributing with new information to improve our understanding of chromosomal evolution and genomic organization in marine perciforms.

scholarly journals A Multidimensional Model for the Vernacular: Linking Disciplines and Connecting the Vernacular Landscape to Sustainability Challenges

2020 ◽  
Vol 12 (16) ◽  
pp. 6347
Author(s):  
Juanjo Galan ◽  
Felix Bourgeau ◽  
Bas Pedroli
Keyword(s):  
Material Culture ◽  
Integrative Approach ◽  
Collective Identities ◽  
Systematic Analysis ◽  
Rural Landscapes ◽  
Landscape Studies ◽  
Wide Range ◽  
The Many ◽  
Sustainability Challenges ◽  
Vernacular Landscape

After developing a systematic analysis of the vernacular phenomenon in different disciplines, this paper presents a flexible model to understand the multiple factors and the different degrees of vernacularity behind the many processes that lead to the generation of material culture. The conceptual model offers an open, polythetic and integrative approach to the vernacular by assuming that it operates in different dimensions (temporal, socio-political, sociological, locational, epistemological, procedural, economic and functional), and that the many attributes or characteristics included in those dimensions are all relevant but not strictly necessary. The model is intended to facilitate a more methodical and rigorous connection between the vernacular concept and contemporary discourses on sustainability, resilience, globalization, governance, and rural-urban development. In addition, and due to its transdisciplinary character, the model will enable the development of comparative studies within and between a wide range of fields (architecture, landscape studies, design, planning and geography). A prospective analysis of the use of the model in rural landscapes reveals its potential to mediate between the protective approach that has characterized official planning during the last decades and emergent approaches that advocate the reinterpretation of the vernacular as a new form to generate new collective identities and to reconnect people and place.

scholarly journals Comprehensive analysis of the association between human diseases and water pollutants

2021 ◽  
Author(s):  
Xu Zhang ◽  
Xiang Zhou ◽  
Xiaolei Zhang ◽  
Xiaohan Ren ◽  
Xinglin Chen ◽  
...  
Keyword(s):  
Drinking Water ◽  
Human Health ◽  
Polybrominated Diphenyl Ethers ◽  
Colonic Neoplasms ◽  
Human Diseases ◽  
Integrative Approach ◽  
Vast Number ◽  
Water Pollutants ◽  
Perfluorinated Chemicals ◽  
The Many

Abstract Drinking water is an important natural resource. For many people around the world, especially in developing countries, access to safe drinking water is still a distant dream. An increasing number of human activities and industrialization have caused various physical, chemical and biological pollutants to enter water bodies, affecting human health. Efforts to figure out an effective method to predict water pollution poisons and human diseases have increased worldwide. Water pollutants contain a vast number of additives such as perfluorinated chemicals, polybrominated diphenyl ethers, phthalate, nanomaterials, insecticides, microcystins, heavy metal and pharmacologies. Here, we explored an integrative approach to identify genes, biological processes, molecular functions, and diseases linked to exposure to these water pollutants. These processes and functions affected by water pollutants are related to the many diseases, including colonic neoplasms, breast neoplasms hepatitis B, bladder cancer and human cytomegalovirus infection. Therefore, conducting an integrative toxicogenomic analysis of water pollutants are more appropriate for evaluating the potential effects of plastics in human health.

A Fruitful Framework: Commentary for a More Integrative Approach

2017 ◽  
Vol 10 (1) ◽  
pp. 123-126 ◽  
Author(s):  
Juliana M. Klein ◽  
Erick P. Briggs
Keyword(s):  
Experiential Learning ◽  
Implicit Bias ◽  
Workplace Discrimination ◽  
Downward Trend ◽  
Integrative Approach ◽  
Historical Events ◽  
Legal Reforms ◽  
Turbulent Times ◽  
The Many

Compared with more turbulent times in history, some might argue workplace discrimination has seen a downward trend. Others would contend that workplace discrimination has “just gone underground” and become more covert (Herring, 2002, p. 13). Either way, not-so-distant historical events such as the landmark Texaco case in 1996 and the Ford Motor case in 2000 remind us that discrimination demands our attention. Calls for research on interventions have surfaced (Becker, Zawadzki, & Shields, 2014), and proposals such as legal reforms, implicit bias training (Bartlett, 2009), and experiential learning workshops have answered (Cundiff, Zawadzki, Danube, & Shields, 2014). The focal article (Jones, Arena, Nittrouer, Alonso, & Lindsey, 2017) contributes to this discussion as it turns our attention to the construct space of discrimination and presents a framework for organizing its facets and forms. Without a doubt, a framework that lends itself to the integration of the many forms of discrimination is long overdue.

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