Effect of a fibrolytic enzyme preparation from Trichoderma longibrachiatum on the rumen microbial population of dairy cows

2002 ◽  
Vol 48 (1) ◽  
pp. 14-20 ◽  
Author(s):  
V L Nsereko ◽  
K A Beauchemin ◽  
D P Morgavi ◽  
L M Rode ◽  
A F Furtado ◽  
...  

The effects of supplementing a dairy cow diet with incremental levels of a fibrolytic enzyme preparation (preparation B) from Trichoderma longibrachiatum on the rumen microbial population were investigated. Two cows fitted with rumen cannulae were each fed a diet containing barley-based concentrate (52%), maize silage (29%), and chopped alfalfa hay (19%), supplemented with 0, 1, 2, 5, or 10 L of preparation B per tonne of dry matter (DM). Preparation B stimulated numbers of total viable bacteria in a quadratic manner (P < 0.05), to approximately 230, 330, 390, and 250% at 1, 2, 5, and 10 L·t–1 DM, respectively. Preparation B increased the numbers of cellobiose-utilizing (P < 0.01), xylanolytic (P < 0.05), and amylolytic bacteria (P < 0.05), but had no effect (P > 0.05) on numbers of cellulolytic bacteria. However, when bacterial numbers enumerated on each substrate were expressed as a proportion of total viable bacterial numbers, only cellobiose utilizers were stimulated, and this stimulation was limited to the 1 L·t–1 DM level of preparation B (P < 0.05). The results of this study demonstrate that the inclusion of an exogenous fibrolytic enzyme preparation in dairy cow diets increased the numbers of rumen bacteria that utilize hemicelluloses and secondary products of cellulose digestion.Key words: rumen, fibrolytic enzymes, cellulase, xylanase, cellulolytic, xylanolytic.

Author(s):  
E.S. Kolver ◽  
J.R. Roche ◽  
D. Miller ◽  
R. Densley

This paper reviews the use of maize silage in pastoral dairying systems in New Zealand. The evolution of dairying systems to make profitable use of maize silage and other supplementary feeds has occurred during the last decade in conjunction with an increased use of maize silage. When used within recommended levels of feeding, maize silage provides a low cost source of starch and fibre which complements pasture well for much of the year. Balancing dietary deficiencies in protein, minerals, and in some cases fibre, will optimise milksolids production at high levels of maize silage supplementation. Targets for good quality maize silage include a dry matter content of 28-35%, an energy content of 10.8 MJME/ kgDM, a protein content of 7-8%, and a pH of 3.8 to 4.5. Opportunities for further productivity gains exist through further intensification of dairying systems using high-yielding crops. Keywords: dairy cow, feed quality, maize silage, nutrition


1999 ◽  
Vol 1999 ◽  
pp. 22-22
Author(s):  
R.L.G. Zom ◽  
E. Kamerman ◽  
G. Remmelink ◽  
G. van Duinkerken

The Dutch DVE/OEB protein evaluation system (Tamminga et al., 1994) gives predictions for the concentration of whole true protein digested in the small intestine (DVE) in cattle feeds, but not for the concentration of single amino acids. Therefore, new standard methods has been introduced for the prediction of true methionine and lysine digested in the small intestine (DVmet and DVlys, respectively) in cattle feeds based on the principles of the calculation of DVE (van Duinkerken and Blok, 1998). These methods give the opportunity to select particular feeds and concentrate ingredients in order to manipulate the concentration of DVmet and DVlys in diets and compound concentrates. However, in the Netherlands, there are no recommendations for DVmet and DVlys in dairy cow rations established yet. An experiment was therefore conducted to study the effects of feeding compound concentrate supplements with either a low (L) or high (H) level of DVmet on feed intake and milk production in dairy cows fed a grass and maize silage mixture ad libitum


2013 ◽  
Vol 29 (4) ◽  
pp. 378-387 ◽  
Author(s):  
L. Baldinger ◽  
W. Zollitsch ◽  
W.F. Knaus

AbstractDuring the winter feeding period in organic dairy production systems in the alpine and pre-alpine regions of Austria and its neighboring countries, maize silage is an energy-rich forage that is regularly included in grass-silage-based diets to improve the energy supply of the cows. Italian ryegrass (Lolium multiflorumLam.) is also a high-energy fodder grass popular as forage for dairy cows, but it is rarely cultivated in Austrian organic agriculture. The two crops differ in their cultivation demands and characteristics. Italian ryegrass establishes rapidly and may reduce the risk of soil erosion. Italian ryegrass would be a beneficial addition to crop rotation, which is an essential tool in successful organic farming. In a 15-week feeding trial, Italian ryegrass silage and maize silage were fed to 22 lactating Holstein dairy cows. Organically produced Italian ryegrass silage and maize silage were included at a rate of 40% [of dry matter (DM)] in grass-silage-based mixed basal diets. The mixed basal diets were supplemented with modest amounts of additional concentrates (2.7–3.0 kg DM day−1). Owing to the higher energy content of maize silage as compared to Italian ryegrass silage, the maize diet provided more energy [6.3 MJ net energy for lactation (NEL) kg−1DM] than the ryegrass diet (6.15 MJ NEL kg−1DM). The protein supply of the maize diet and the ryegrass diet was intended to be equal, but in fact the protein content of the maize diet was significantly lower (122 g crude protein kg−1DM) than that of the ryegrass diet (141 g kg−1DM). When the maize diet was fed, feed intake, milk yield and milk protein content were significantly higher as compared to the ryegrass diet. Also, intake of crude protein was significantly lower when feeding the maize diet, and in combination with the higher milk protein yield, this enabled an efficiency of gross nitrogen (N) utilization as high as 0.304. This level of N efficiency can be considered as above average and was significantly and considerably higher than the level of 0.259 observed when the ryegrass diet was fed. Therefore, maize silage upholds its reputation as an ideal energy-rich component in grass-silage-based dairy cow diets.


1992 ◽  
Vol 32 (3) ◽  
pp. 293 ◽  
Author(s):  
JB Moran

Dairy cows in mid lactation were pen-fed ad libitum maize silage, ad libitum maize greenchop, or restricted maize greenchop. A cottonseed meal supplement was also fed at 20% forage maize DM. The maize greenchop was harvested daily over 5 weeks during February and March. Both forms of forage maize were fed to sheep in metabolism cages. Digestibility was always higher with maize silage. Digestibility of maize greenchop improved with time until 34-36% DM, after which it declined, this being associated with changes in structural carbohydrates in the crop. Cows fed maize silage produced more milk and gained less weight than cows fed both greenchop diets. In a concurrent field trial, cows grazed irrigated perennial pastures in late summer and were offered 7-8 kg DM/day of the silage or greenchop. Additional cows were allocated extra pasture with no supplement. Treatment differences in milk production and liveweight change were not significant. There were differences in rumen metabolism between diets in both pen-fed and grazed cows; rumen ammonia levels increased during the day in cows fed maize silage, but they decreased or remained constant when maize greenchop was fed. There was more maize grain in greenchop at physiological maturity than in silage, and this could have contributed to observed differences in performance and rumen metabolism. The low rumen ammonia levels, and the fact that forage maize was higher in energy than the pasture on offer, suggest that milk response would have improved with the inclusion of a rumen-degradable nitrogen source such as urea with the forage maize supplements.


2013 ◽  
Vol 22 (1) ◽  
pp. 70-92 ◽  
Author(s):  
Tim Keady ◽  
Seamus Hanrahan ◽  
Christina Marley ◽  
Nigel David Scollan

This paper reviews the production of, and factors affecting the performance of dairy cows, beef cattle and sheep offered silage based diets in Ireland and UK. Digestibility is the most important factor influencing the feed value of grass silage and consequently animal performance. Each 10 g kg-1 increase in digestive organic matter in the dry matter (DOMD) increases milk yield of dairy cows by 0.33 kg d-1, carcass gain of beef cattle by 23.8 g d-1 , carcass gain of finishing lambs by 9.3 g d-1, lamb birth weight by 52.3 g and ewe weight post lambing by 1.3 kg, respectively. Factors influencing feed value of grass silage are discussed including harvest date, wilting, fertilizer management, chop length and use of additives at ensiling. Maize silage increases the performance of cattle and sheep whilst whole crop wheat silage has no beneficial effect. Advances in silage technology, has enabled the ensiling high protein forages, such as red clover, lucerne and kale.


2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Patrycja Rajtar ◽  
Marek Sady ◽  
Paweł Górka ◽  
Sylvia Kehoe ◽  
Piotr Micek

Abstract Grain from traditional varieties of rye is not commonly used in dairy cattle nutrition. However, new hybrid varieties of rye currently available are characterized by some nutritional and agrotechnical benefits. This paper deals with the hypothesis that rye grain derived from a hybrid variety may be an alternative for maize grain in diets for dairy cattle. Sixteen lactating Polish Holstein-Friesian cows were divided into two groups according to their parity (8 primi- and 8 multiparous), stage of lactation (106 ± 30 days after calving) and milk yield (34 ± 4 kg/day). Cows were fed a total mixed ration (TMR) containing grass silage and whole crop maize silage and 29.2% of the concentrate (in dry matter). The latter contained approximately 48% of either maize grain (M) or hybrid rye grain (HR) as a main source of cereal grain. Experimental diets were fed for 9-weeks. The use of HR as a substitute for M did not affect (P>0.05) dry matter intake and milk yield. There were no differences between treatment groups in the content of milk solids, amino acids, and fatty acid profile. However, substituting M by HR positively influenced composition of milk protein fractions by increasing the proportion of α-casein (37.0 vs 39.7%; P<0.01) and к-casein (6.5 vs 7.3%; P=0.02) as well as decreasing the proportion of β-casein (28.8 vs 27.8%; P=0.02) and sensory characteristics of the milk (body and texture and taste; P<0.05). In turn, the composition of the diet did not affect the technological suitability for processing of milk fat (acid and peroxide number, melting and solidification temperature), rennet coagulation time, heat stability or titratable acidity. This study has shown that hybrid rye grain may be an alternative for maize grain in a TMR based on grass and whole maize silage for mid-lactation dairy cows. Further studies are needed with higher proportion of hybrid rye grain in TMR or with other roughages used in a basal diet to fully determine efficiency of hybrid rye grain use in diets for dairy cows.


1997 ◽  
Vol 64 (2) ◽  
pp. 181-195 ◽  
Author(s):  
FRANCIS ENJALBERT ◽  
MARIE CLAUDE NICOT ◽  
CORINE BAYOURTHE ◽  
MICHELE VERNAY ◽  
RAYMOND MONCOULON

Dairy cows fitted with ruminal, duodenal and ileal cannulas were utilized to investigate the effects of feeding with Ca soaps (CaS) of palm fatty acids (FA) and rapeseed FA. Diets compared were control diet based on maize silage and concentrate, and two diets with 40 g CaS of palm oil FA or rapeseed oil FA/kg diet, replacing part of the concentrates of the control diet. Total digestibilities of dry matter, fibre and fat, and ruminal fermentation were not significantly altered by giving CaS; the extent of ruminal biohydrogenation of total unsaturated C18 FA was significantly reduced by both CaS diets. Apparent intestinal digestibility of FA was not different among diets, although the amount of FA absorbed with the CaS diets was twice that with the control diet. No difference among diets was observed for milk production, or fat and protein contents. Giving CaS diets decreased the proportions of 4[ratio ]0 to 14[ratio ]0 FA in milk fat, and increased cis-18[ratio ]1n−9, compared with control diet. The rapeseed diet lowered the content of 16[ratio ]0, and increased the contents of 18[ratio ]0 and trans-18[ratio ]1n−7. CaS diets did not result in a marked increase of polyunsaturated FA content in milk fat. Butter from cows fed on the CaS diets contained more liquid fat at 6 and 14°C than butter from the cows fed on the control diet. Incorporating CaS, particularly those from rapeseed, in dairy cows' diets increased C18 FA in milk and improved butter spreadability.


1996 ◽  
Vol 36 (7) ◽  
pp. 771 ◽  
Author(s):  
CR Stockdale

Friesian cows (16) in late lactation grazed pure white clover (Trifolium repens cv. Haifa) swards for 32 days in autumn and were supplemented with maize (Zea mays) silage. Four groups of 4 cows were offered either 19 or 39 kg dry matter (DM)/cow.day of white clover with either 0 or 4.4 kg DM/cow.day of maize silage. All cows were individually fed maize silage and grazed the pasture as individual groups according to treatment. When maize silage was fed, total intake increased, this occurring to a greater degree at the low pasture allowance. The level of substitution of silage for pasture was 0.14 and 0.40 kg DM reduction in pasture intake per kg DM of maize silage eaten at the low and high allowances, respectively. At the low pasture allowance, milk yields were 10.1 and 13.7 kg/cow. day when 0 and 4.4 kg DM/cow.day of maize silage were fed, respectively, and were 15.5 and 15.9 kg/cow.day at the high pasture allowance. Liveweight and body condition increased as plane of nutrition increased but there were no effects of feeding on milk fat or protein contents. Feeding maize silage had little effect on any rumen or faecal variable although there was generally less ammonia nitrogen in rumen fluid when cows were supplemented with maize silage.


1995 ◽  
Vol 35 (1) ◽  
pp. 19 ◽  
Author(s):  
CR Stockdale

Eight experiments were conducted over 2 years with 92 lactating dairy cows individually fed various combinations of irrigated perennial pasture and maize silage in an indoor feeding facility. Responses to different amounts of maize silage and pasture in early and late lactation were measured. Daily pasture intake ranged from 6.2 to 12.4 kg dry matter (DM)/cow, while maize silage intake ranged from 0 to 12.4 kg DM/cow. The marginal return to feeding maize silage at up to 5 kg DM/cow.day to cows in early lactation eating about 7 kg DM/day of pasture as their basal ration was 0.89 kg extra milk for each kg DM maize silage eaten. In late lactation, this was reduced to 0.63 kg milk/kg DM. Milk yield responses to maize silage at both stages of lactation were lower at higher levels of pasture feeding. Although milk fat content was maintained over a wide range of maize silage, feeding large quantities of maize silage in early lactation was detrimental to milk production. The likely explanation for poor animal performance at the high levels of maize silage was dietary protein insufficiency.


Author(s):  
S.L. Woodward ◽  
A.V. Chaves ◽  
G.C. Waghorn ◽  
P.G. Laboyrie

Forages suitable for supplementing pasture-fed dairy cows over summer-autumn must provide adequate dry matter (DM) and increase milk yield above that produced by cows grazing normal ryegrass/white clover pasture, without compromising milk composition. A trial was conducted in January-February 2001 to compare the benefits obtained from feeding four types of silage. There were two silages that contained condensed tannins (CT) (birdsfoot trefoil (Lotus corniculatus) and sulla (Hedysarum coronarium)), maize silage or traditional ryegrass-dominant pasture silage, all fed at 5 kg DM/cow/d with restricted pasture. Cows on the restricted pasture (control) treatment and those fed the silage treatments were offered an allowance of 25 kg DM pasture/cow/d, while the full pasture cows were offered 50 kg DM pasture/cow/d. Silage supplementation, regardless of silage type, increased both total dry matter intake (DMI) and milk production compared with the restricted pasture treatment. Cows on the Lotus silage supplement, and the full pasture treatment had significantly higher milk yields than the other silage-supplemented cows, all of which had similar milk yields. For the Lotus silage treatment, the high milk yield was probably due to a combination of the higher nutritive value of the silage, and to the action of CT, because the total DMI of the cows on the Lotus silage was the same as that of cows on the pasture silage and maize silage. The high milk yield of the full pasture treatment was mainly a result of the cows having a significantly higher total DMI than cows on all the other treatments. This trial has demonstrated the potential benefit of silage supplementation, particularly with Lotus silage, for increased milksolids yield in summerautumn when low pasture growth rates and quality may otherwise limit production. Keywords: condensed tannins, dairy cows, Lotus, maize, milksolids, ryegrass, silage, sulla, supplementation


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