Variation in Piceaglauca seed germination associated with the year of cone collection

The effects of cone storage period and pregermination treatment on seed maturity and dormancy were compared for cones of white spruce (Piceaglauca (Moench) Voss) collected from individual trees in 1984 and 1988. Seeds were extracted from cones and germinated after 2 or 6 weeks of cone storage in 1984 and after 2, 4, 6, 10, or 14 weeks in 1988. Based on cumulative degree-days, seeds were more mature at collection time in 1988 than in 1984. Seeds from 1984 cones stored for 6 weeks matured during storage, and both germination percentage (GP) and rate of germination (GR) were significantly improved. In contrast, storage up to 14 weeks in 1988 did not increase GP and GR, as seed had attained maturity prior to cone collection. Seed dormancy was present in both 1984 and 1988. Significant improvements in GP and GR were achieved in 1984 with a pregermination treatment even before seed maturity was attained. Prechilling of seed after 6 weeks of cone storage increased GP from 60 to 95% in 1984 and 64 to 89% in 1988.

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Effects of different treatments on seed dormancy breaking and germination in Acer cappadocicum Gleditsch var. cappadocicum

Šumarski list ◽

10.31298/sl.144.3-4.5 ◽

2020 ◽

Vol 144 (3-4) ◽

pp. 159-166

Author(s):

Hanife Erdogan Genç ◽

Ali Ömer Üçler

Keyword(s):

Seed Germination ◽

Seed Dormancy ◽

Germination Percentage ◽

Growth Chamber ◽

Dormancy Breaking ◽

Seed Collection ◽

Physiological Dormancy ◽

Germination Value ◽

Positive Effect ◽

Collection Time

This study was carried out to determine effects of different pretreatment on seed germination and to overcome dormancy in Acer cappadocicum seeds. The seeds were collected in 2008 three times with aproximately 15-days intervals. In order to overcome dormancy, several germination treatments were applied. The treatments were (1) different seed collection time, (2)soaking in water, (3) cold-moist stratification and (4) GA3 (gibberellic acid) application. The treated seeds were germinated in growing chamber at 5 0C and in greenhouse conditions. This research showed that seeds of Acer cappadocicum exhibit physiological dormancy and require stratification period to overcome seed dormancy. The highest germination percentage in the growing chamber subjected to GA3 process after eight weeks of stratification treatment was 62 % for Acer cappadocicum seeds. The highest germination percentage in greenhouse was obtained with cold stratification after eight weeks (95 %). It was found out that GA3 treatment had a significant effect on germination in growth chamber + 5 0C but GA3 treatment didn’t have a significant effect on germination in greenhouse conditions. GA3 treatment and soaking of unstratified seeds in water for 48 hr didn’t have any positive effect on germination value in greenhouse conditions. Although growth chamber and green house results both indicated that seed collection time did not seem to play a role as statistically on seed germination, Duncan’s test showed that the third seed collection time was in a different group.

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Factors Affecting Seed Dormancy and Germination of Greater Bur-Parsley (Turgenia latifolia)

Planta Daninha ◽

10.1590/s0100-83582018360100125 ◽

2018 ◽

Vol 36 ◽

Author(s):

M. REZVANI ◽

S.A. SADATIAN ◽

H. NIKKHAHKOUCHAKSARAEI

Keyword(s):

Seed Germination ◽

Environmental Factors ◽

Seed Dormancy ◽

Seedling Emergence ◽

Potassium Nitrate ◽

Germination Percentage ◽

Dormancy Breaking ◽

Planting Depth ◽

Factors Affecting ◽

Drought And Salt Stresses

ABSTRACT: Our knowledge about seed dormancy breaking and environmental factors affecting seed germination of greater bur-parsley (Turgenia latifolia) is restricted. This study has addressed some seed dormancy breaking techniques, including different concentrations of gibberellic acid (GA3) and potassium nitrate (KNO3), leaching duration, physical scarification as well as some environmental factors effective on seed germination such as salt and drought stresses, pH and seed planting depth. Seed germination was promoted with lower concentrations of KNO3 (0.01 to 0.02 g L-1), while higher concentrations reduced germination percentage. Seed dormancy was declined by low concentrations of GA3 up to 100 ppm. Seeds of greater bur-parsley germinated in a range of pH from 3 to 7. With enhancement of drought and salt stresses, seed germination decreased. Also, there was no seed germination in a high level of stresses. Seedling emergence reduced as planting depth increased. Use of GA3, KNO3, leaching and physical scarification had a positive effect on seed dormancy breaking of greater bur-parsley. The information from the study increases our knowledge about seed dormancy breaking techniques, response of germination to drought and salt stresses and also determination of distribution regions of greater bur-parsley in the future.

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Enhanced germination performance of dormant seeds of Eragrostis tef in the presence of light

Tropical Grasslands - Forrajes Tropicales ◽

10.17138/tgft(7)244-251 ◽

2019 ◽

Vol 7 (3) ◽

pp. 244-251

Author(s):

Iskender Tiryaki ◽

Sakir Anil Kaplan

Keyword(s):

Seed Germination ◽

Seed Dormancy ◽

Plant Hormones ◽

Indole Acetic Acid ◽

Germination Percentage ◽

Eragrostis Tef ◽

Crop Establishment ◽

Giberellic Acid ◽

Related Plant ◽

Time Required

Lack of germination or low germination due to seed dormancy prevents successful crop establishment in several important plants. To determine the presence of innate seed dormancy and effects of stress-related plant hormones on germination performance of Teff (Eragrostis tef) seeds, we primed seeds in 1% KNO3 for 24 hours in dark conditions at 21 ± 0.5 ºC along with varying concentrations of chemicals known to influence seed germination, including: acetyl salicylic acid (ASA); methyl jasmonate (JA-Me); giberellic acid (GA3); and indole acetic acid (IAA). Primed seeds were incubated either in constant light (210 µM/m2/s) or in darkness at 21 ± 0.5 ºC. The results indicated that primingsignificantly improved final germination percentage (FGP) in both light (92.5%) and dark (89.4%) conditions compared with untreated seeds. The inclusion of plant hormones in the priming media generally had limited effects, except for 10 mM ASA (94.5%) and 100 mM GA3 (92.5%). ASA generally provided faster seed germination than seeds primed in 1% KNO3 only, while the other plant hormones had no effect on the time required for 50% of FGP in the dark. Priming had no significant effect on time span of germination in either light or dark incubation conditions. The results demonstrate that E. tef has light-inducible seed germination and about half of freshly harvested seeds can be dormant, which can be eliminated to some extent by priming seeds in 1% KNO3.

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The Effect of Sodium Hypochlorite (NaOCl) and Heat Treatments on Seed Germination of Rice: An Approach to Restore Seed Viability

Journal of Experimental Agriculture International ◽

10.9734/jeai/2021/v43i530689 ◽

2021 ◽

pp. 49-53

Author(s):

Monoj Sutradhar ◽

Subhasis Samanta ◽

Brijesh Kumar Singh ◽

Md. Nasim Ali ◽

Nirmal Mandal

Keyword(s):

Seed Germination ◽

Seed Dormancy ◽

Sodium Hypochlorite ◽

Seed Viability ◽

Germination Percentage ◽

Heat Treated ◽

Mother Plants ◽

Breaking Seed Dormancy ◽

One Year

Dormancy in rice serves as a mechanism of survival by protecting the seed from germinating in the mother plants; however, it becomes a problem in germination during sowing in soil or under in vitro conditions. This study was conducted to determine the effect of heat treatment and sodium hypochlorite (NaOCl) treatment of seeds on dormancy alleviation. The seeds included both freshly harvested seeds and one-year-old stored seeds, which were tested for germination after different types of seed treatments. Both the treatments increased the germination percentage in seeds, however, it was lesser in the case of old seeds. The best results were obtained from 2% NaOCl treatment for 24 hrs in new seeds, i.e. 92.84±0.103 % germination percentage (GP). However, the higher GP in old seeds were obtained from 48 hrs of heat-treated seeds i.e. 82.9±0.509 % GP. The results of the experiment revealed that rice seeds start to lose viability within a year due to seed dormancy, but this can be reversed with proper measures. These methods of breaking seed dormancy can be considered effective to break seed dormancy and improve seed germination in rice.

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Storage Period and Different Abiotic Factors Regulate Seed Germination of Two Apocynum Species — Cash Crops in Arid Saline Regions in the Northwestern China

Frontiers in Plant Science ◽

10.3389/fpls.2021.671157 ◽

2021 ◽

Vol 12 ◽

Author(s):

Li Jiang ◽

Chaowen She ◽

Changyan Tian ◽

Mohsin Tanveer ◽

Lei Wang

Keyword(s):

Seed Germination ◽

Abiotic Stresses ◽

Arid Regions ◽

Natural Fiber ◽

Abiotic Factors ◽

Light Condition ◽

Storage Period ◽

Germination Percentage ◽

Environmental Benefits ◽

Degraded Land

On degraded land in arid regions, cultivation of Apocynum species can provide significant environmental benefits by preventing soil erosion and desertification. Furthermore, Apocynum venetum and Apocynum pictum, which are mainly distributed in salt-barren lands in the northwestern region of China, are traditionally used to produce natural fiber and herbal tea. Direct sowing of both species may encounter various abiotic stresses such as drought and salinity. However, these effects on germination remain largely unknown, especially for seeds with different storage periods. The aim of this study was to evaluate the effects of storage period, light condition, temperature regime, drought, and salinity on germination performances of both species. Germination experiment was carried out in November 2017. There were four replicates for each treatment, and each petri dish contained 25 seeds. The results indicated that prolongation of storage period significantly decreased the germination percentage and velocity, especially under abiotic stresses. Light did not affect seed germination of A. venetum and A. pictum under any conditions. Seeds had better germination performance at 10/25 and 15/30°C than those of seeds incubated at any other temperatures. With the increase of polyethylene glycol (PEG) and salinity concentrations, seed germination for both species gradually decreased, especially for seeds stored for 2 years. Low PEG (0–20%) and salinity concentration (0–200 mM) did not significantly affect germination percentage of freshly matured seeds. However, long-time storage significantly decreased drought and salinity tolerance in A. venetum and A. pictum during germination stage. For saline soils in arid and semi-arid regions, freshly matured seeds or 1-year-stored seeds of both Apocynum species are recommended to be sown by using drip-irrigation in spring.

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Effects of Temperature, Scarification, Stratification, Phytohormones, and After-Ripening on the Dormancy and Germination of Eucommia ulmoides Oliv. Seeds

Forests ◽

10.3390/f12111593 ◽

2021 ◽

Vol 12 (11) ◽

pp. 1593

Author(s):

Shiming Deng ◽

Zhijun Deng ◽

Xiaofeng Wang ◽

Hai Lu ◽

Hua Xue

Keyword(s):

Seed Germination ◽

Water Absorption ◽

Seed Dormancy ◽

Seed Coat ◽

Underlying Mechanism ◽

Germination Percentage ◽

Growth Potential ◽

Eucommia Ulmoides ◽

Germplasm Resources ◽

Type 3

Eucommia ulmoides Oliv., the only member of the family Eucommiaceae, is endemic to China and has great development and utilization prospects. The seeds of E. ulmoides show dormancy but the underlying mechanism remains unknown. The aim of this study was to determine the cause of the seed dormancy and provide fundamental knowledge for the breeding, genetic improvement, and conservation of the germplasm resources of this species. According to the seed dormancy classification system developed by Jerry M. Baskin and Carol C. Baskin, we compared the germination percentage between intact seeds and isolated embryos, constructed water absorption curves, and evaluated the germination of seeds treated with scarification, cold/warm-moist stratification, after-ripening during dry storage, and gibberellic acid (GA3). The results showed that the intact seeds germinated only at 10 °C with a low germination percentage of 13.3% whereas the isolated embryos had a high normal germination percentage among a wider range of temperatures. According to the results from the scarified seeds, half seeds, and intact seeds, the seed coat significantly restricted the embryo water absorption. The scarification, after-ripening, cold/warm-moist stratification, and GA3 treatments promoted seed germination. Among them, cold-moist stratification was the most effective method and the temperature range of seed germination increased in both directions from 10 °C with prolonged stratification. The germination percentage increased significantly at constant temperatures with the highest germination percentage of 93.7 ± 0.3% at 10 °C and a light/dark cycle after 90 days of cold-moist stratification. Therefore, the freshly harvested E. ulmoides seeds exhibited a combinational dormancy comprising physical and Type 3 non-deep physiological dormancy, causing limited embryo water absorption by the seed coat and a low embryo growth potential. Given the unique phylogenetic characteristics and utility of E. ulmoides, our findings should promote studies of seed dormancy evolution and the development and application of E. ulmoides germplasm resources.

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Pre-Germination Treatments, Temperature, and Light Conditions Improved Seed Germination of Passiflora incarnata L.

Agriculture ◽

10.3390/agriculture11100937 ◽

2021 ◽

Vol 11 (10) ◽

pp. 937

Author(s):

Luciana G. Angelini ◽

Clarissa Clemente ◽

Silvia Tavarini

Keyword(s):

Seed Germination ◽

Seed Dormancy ◽

Large Scale ◽

Seed Quality ◽

Central Italy ◽

Germination Percentage ◽

Climatic Conditions ◽

Added Value ◽

Medicinal And Aromatic Plants ◽

Passiflora Incarnata

Perennial medicinal and aromatic plants (MAPs) may represent interesting, environmentally friendly crops for the Mediterranean environments. Among MAPs, Passiflora incarnata L. (maypop) represents a very promising crop for its wide adaptability to diverse climatic conditions, low input requirements, and high added-value due to its unique medicinal properties. The main problem in P. incarnata large-scale cultivation is the poor seed quality with erratic and low seed germination, due to its apparent pronounced seed dormancy. Therefore, the aim of this work was to investigate different chemical and physical treatments for overpassing seed dormancy and enhancing seed germination rates of P. incarnata. The effects of (i) different pre-germination treatments (pre-chilling, gibberellic acid—GA3, leaching, scarification, non-treated control), (ii) light or darkness exposure, and (iii) temperature conditions (25, 30, and 35 °C constant and 20–30 °C alternating temperatures) have been examined in seed germination percentage and mean germination time of three P. incarnata accessions (F2016, FF2016, and A2016) grown in field conditions in Central Italy. Data showed that the pre-germination treatments generally stimulated faster germination compared to the control, with the best results obtained in the dark and with high temperatures. These findings are useful for the choice of the most suitable seed pre-germination treatment that can facilitate stable, high and agronomically acceptable germination rates in P. incarnata.

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Morfología de semilla, germinación y desarrollo postemergente de tres especies del género Polianthes L. (Agavaceae)

Botanical Sciences ◽

10.17129/botsci.1611 ◽

2017 ◽

pp. 55

Author(s):

Héctor Serrano-Casas ◽

Eloy Solano-Camacho ◽

Adelaida Ocampo-López

Keyword(s):

Seed Germination ◽

Seed Dormancy ◽

Seed Viability ◽

Germination Percentage ◽

Seed Morphology ◽

Seedling Development ◽

The Other ◽

Viability Test ◽

Other Hand ◽

Germination Process

The seed germination process and seedling development were studied for Polianthes geminiflorn (Lex.) Rose var. geminiflom, Polianthes sp . and P. longiflom Rose. The seed viability test estimated was from 94 to 100%. In seeds treatment by soaking the seeds in water at 25ºC during three, six and twelve hours, the germination percentage, velocity and germination uniformity were increased like control assay. On the other hand, P geminiflorn var. geminiflora. were more resistent than Polianthes sp. and P. longiflora. seeds to a treatment of three, six and twelve hours in water a t 40ºC. The three species had epigeal germination and their cotyledon developed a haustorium. None of this species exhibited seed dormancy and they were orthodox. There were significant differences in the first leave length between the three species. The seed morphology, germination behavior and seedlings development were very similar.

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Seed dormancy removal treatments and germnaton characterstcs of Acer trautvetteri Medvedev seeds

Šumarski list ◽

10.31298/sl.144.9-10.3 ◽

2020 ◽

Vol 144 (9-10) ◽

pp. 473-473

Author(s):

Hanife Erdogan Genç ◽

Ali Ömer Üçler

Keyword(s):

Seed Dormancy ◽

Germination Percentage ◽

Climatic Conditions ◽

Black Sea Region ◽

Seed Collection ◽

Germination Characteristics ◽

Significant Difference ◽

Physiological Dormancy ◽

Negative Effect ◽

Collection Time

In this study, seed dormancy removal and germination characteristics of Acer trautvetteri Medvedev, is one of the major maple species of the eastern Black Sea region, were studied. To do that, the seeds were collected in 2008 three times with aproximately 15-days intervals. In order to remove dormancy, several germination treatments were applied. The treatments were (1) different seed collection time, (2) soaking in water, (3) cold-moist stratification and (4) GA3 (gibberellic acid) application. The treated seeds were germinated in growing chamber at 5 0C and in greenhouse conditions. This research indicated that seeds of Acer trautvetteri exhibit physiological dormancy and require stratification period to remove seed dormancy. The highest germination percentage in the growing chamber subjected to GA3 application after eight weeks of stratification treatment was 66 % for Acer trautvetteri seeds. The highest germination percentage in greenhouse was obtained with cold stratification after eight weeks (81 %). Although GA3 treatment had a positive effect on germination in growth chamber + 5 0C, GA3 treatment had a negative effect on germination in greenhouse conditions. Soaking in water of nonstratified seeds wasn’t any significant difference on seed germination. There was a positive correlation between seed collection time and germination (r=0.59). As a result, the third collection time (in october) should be preferred as seed collection time in Acer trautvetteri seeds, considering that it may vary according to the climatic conditions of the year.

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Seed Germination of Huagaimu, a Critically Endangered Plant Endemic to Southeastern Yunnan, China

HortTechnology ◽

10.21273/hortsci.19.2.427 ◽

2009 ◽

Vol 19 (2) ◽

pp. 427-431 ◽

Cited By ~ 10

Author(s):

Yan-Ling Zheng ◽

Wei-Bang Sun

Keyword(s):

Seed Germination ◽

Seed Dormancy ◽

Germination Percentage ◽

Critically Endangered ◽

Dichlorophenoxyacetic Acid ◽

Naphthaleneacetic Acid ◽

Combined Treatments ◽

Endangered Tree ◽

Moist Chilling ◽

2,4 Dichlorophenoxyacetic Acid

As a critically endangered tree in the Magnoliaceae family, huagaimu (Manglietiastrum sinicum) is represented by only 10 mature individuals in evergreen broadleaved montane forests of southeastern Yunnan Province, China. Our previous work revealed the existence of a seed dormancy period for this species. The current study was performed to evaluate the effects of plant growth regulators (PGRs) and moist chilling on breaking seed dormancy in this species. Germination of seeds pretreated for 24 h with gibberellic acid (GA3), α-naphthaleneacetic acid, 6-benzyladenine, and 2,4-dichlorophenoxyacetic acid indicated that only GA3, at concentrations of 300 and 500 mg·L−1, can significantly break the seed dormancy of huagaimu after 50 days of incubation, with about 66% germination under 500 mg·L−1 GA3. Moist chilling at 4 °C for 3 weeks can also effectively break the seed dormancy of the species, with 56% of seeds treated in this way germinating after 30 days of incubation. The combined treatments of PGRs followed by moist chilling were also conducted. Based on germination results after 30 days of incubation, the seed germination of combined treatments was significantly higher than that of PGR treatments. However, the seeds treated only with moist chilling presented the highest germination percentage among all the treatments.

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