THE INFLUENCE OF ENVIRONMENTAL TEMPERATURE AND HUMIDITY ON SHOCK PRODUCED BY A CLAMPING PROCEDURE
The influence of environmental temperature on the development of shock produced by a clamping technique in rats was studied. In experiments in which the animals were subjected to different environmental temperatures during the period of limb ischemia, the best survival was obtained with an air temperature of 15 °C. At 9.5 °C and 40 °C the rats did not survive the 10-hour clamping period. When the clamping was carried out at a standard temperature (27 °C) and the rats were then transferred to a room at different temperatures just prior to clamp release, the best survival was obtained at or near a temperature of 24 °C. The temperature in the colon of the shocked rats fell quickly in a cooler environment and rose in a warmer one. When chlorpromazine (0.35 mg/100 g rat) was given at the time of clamp removal to rats kept thereafter at 9.4 °C, 20–21 °C, 24 °C, and 30 °C, survival was significantly prolonged at air temperatures of 20–21 °C, but not at 9.4 °C, 24 °C, or 30 °C. Changes in humidity had no significant influence on survival. The experiments show that the optimum temperature during the period of ischemia is different from that for hindering the development of shock following a period of ischemia.