The influence of adaptation of rumen microflora on in vitro digestion of different forages by sheep and red deer

2002 ◽  
Vol 80 (11) ◽  
pp. 1930-1937 ◽  
Author(s):  
Iain J Gordon ◽  
F Javier Pérez-Barbería ◽  
Paloma Cuartas
Keyword(s):  
Cervus Elaphus ◽  
Red Deer ◽  
In Vitro Digestion ◽  
Ovis Aries ◽  
In Vitro Digestibility ◽  
Rumen Microbes ◽  
Digestion Technique ◽  
Digestion Efficiency ◽  
Rumen Microflora

The rumen microflora ecosystem adapts to the diet consumed by the animal. We tested the extent to which this adaptation facilitates or retards the digestion of plant-based forages. Following adaptation of sheep (Ovis aries) and red deer (Cervus elaphus) to diets containing different mixtures of alfalfa, grass, and heather (a dwarf shrub), an in vitro digestion technique was used to compare the ability of the rumen microflora to digest the mixtures of substrates to which they were adapted with their ability to digest different mixtures of the same substrates. In vitro digestion of different substrates was slightly greater in rumen liquor derived from sheep than in that derived from red deer for each of the different substrates, but the effect was not significant. Digestibility in sheep was independent of how the feed was presented (diet of equal proportions of alfalfa, grass, and heather in each meal (D-EQ): mean in vitro digestibility = 37.3%; alfalfa, grass, and heather presented sequentially on different days (D-SEQ): mean in vitro digestibility = 37.7%, SE of differences = 1.30%, p > 0.05). However, in red deer there was a significant effect of method of diet presentation (D-EQ: mean in vitro digestibility = 36.9%; D-SEQ: mean in vitro digestibility = 34.2%, SE of differences = 1.30%, p < 0.05), digestibility being substantially lower for D-SEQ than for D-EQ. Overall, the results demonstrated that whilst there were no species-specific differences in overall digestion efficiency, dietary adaptation had an effect on substrate digestion efficiency, with rumen microbes adapted to high-quality diets digesting these more efficiently than low-quality diets.

INTERACTION BETWEEN INOCULUM DONOR DIET AND SUBSTRATE IN IN VITRO RUMINANT DIGESTION STUDIES

1966 ◽  
Vol 46 (2) ◽  
pp. 91-95 ◽  
Author(s):  
J. E. Knipfel ◽  
J. E. Troelsen
Keyword(s):  
Organic Matter ◽  
Dry Matter ◽  
In Vitro Digestion ◽  
Barley Grain ◽  
Absolute Amount ◽  
In Vitro Digestibility ◽  
Promoting Effect ◽  
Digestion Technique ◽  
The Difference

Six wethers with rumen fistula were each fed a different combination of alfalfa hay, wheat straw, and barley grain. The same mixtures were fermented for periods of 12 and 48 hours by inoculum from each wether, using a two-stage in vitro digestion technique. The in vitro digestibility of dry matter and organic matter was determined on each treatment combination.The digestibility data revealed interactions among inoculum donor diets, in vitro substrate mixtures, and fermentation periods. When the diet lacked one or more of the substrate components, a 12-hour artificial rumen fermentation resulted in digestibilities that decreased as the composition of the substrate deviated increasingly from that of the donor diet. This effect was eliminated by extending the fermentation period to 48 hours. When all the substrate ingredients were included in the donor diet, the absolute amount of each ingredient, within 10 to 80%, was of less importance.Indications were that alfalfa possessed a specific growth-promoting effect on the microflora.Increasing the proportion of alfalfa in the substrate increased the difference between the in vitro digestibility of dry matter and organic matter. This was suggested to result from a higher concentration of soluble ash in the alfalfa fraction of the mixtures.

scholarly journals Effect of balanced supplementary feeding in winter on qualitative and quantitative changes in the population of microbes colonizing the rumen of red deer

2018 ◽  
Vol 74 (2) ◽  
pp. 6072-2018 ◽  
Author(s):  
SEBASTIAN GNAT ◽  
ROMAN DZIEDZIC ◽  
ANETA NOWAKIEWICZ ◽  
PRZEMYSŁAW ZIĘBA ◽  
ALEKSANDRA TROŚCIAŃCZYK ◽  
...  
Keyword(s):  
Cervus Elaphus ◽  
Red Deer ◽  
Bacterial Flora ◽  
The Body ◽  
Supplementary Feeding ◽  
Growth Media ◽  
Natural Plant ◽  
Rumen Microbiota ◽  
Rumen Microbes

Ruminants are a group of animals that process and assimilate their food in a unique manner. The functioning of the digestive tract of these animals is closely related to the abundance and composition of microbes in the forestomach, which is a complex ecosystem of bacteria, protozoa and fungi. Microorganisms present in the rumen, and in particular their effect on physiological processes in the body, influence the animal’s physical condition and state of health. Microbiological examination of rumen microbiota ecology is hindered by a lack of selective growth media, as well as by difficulties in isolating bacteria in vitro and accurately identifying them. The aim of the study was to evaluate the effect of food consumed by red deer (Cervus elaphus) on the diversity of their rumen microbiota. Microbes were compared in two study periods. In autumn the animals’ diet came exclusively from natural plant sources, while in winter, supplementary feeding was introduced, including specially prepared fodder. The study showed that in deer that did not receive the special fodder in winter, but only natural plant components, the abundance of bacterial flora decreased significantly compared with what it was in autumn, unlike in animals that did receive the fodder, whose composition and caloric value substantially increased the activity of rumen microbes. In winter, changes in proportions of different morphological forms of rumen bacteria were observed, as well as a decline in their total number, particularly in the animals that did not receive the pellets. A similar decline was also observed in the populations of yeasts and protozoa in winter. To sum up the results of the study, the use of the specially prepared high-calorie fodder in winter was shown to influence the rumen ecosystem of red deer. The most significant factor improving the condition of deer receiving supplementary fodder during this period is the stabilization of bacterial flora in the rumen, which directly contributes to the efficiency of digestion..

scholarly journals Cellulase digestion technique compared with the in vitro digestibility of forages

1976 ◽  
Vol 48 (2) ◽  
pp. 187-194
Author(s):  
Seppo Pulli
Keyword(s):  
Protein Content ◽  
Analytical Techniques ◽  
In Vitro Digestion ◽  
Plant Protein ◽  
In Vitro Digestibility ◽  
Pure Stand ◽  
Couch Grass ◽  
Cellulase Digestion ◽  
Digestion Technique

A red clover-grass mixture and a couch grass (E. repens) pure stand were studied at eighteen different stages of maturity in Viikki. The analytical techniques were a cellulase digestion method and a two stage in vitro digestion technique. A high correlation (r = .99*** for both types of stand and residual standard deviations (RSD) of 0.9 and 1.3 respectively) was found between cellulase DM digestibility and in vitro DM digestibility. The difference between cellulase and in vitro DM digestibility increased as the plants advanced in maturity, because the effect of enzyme decreased, as the plants were more matured. The relationship between cellulase DM digestibility and plant protein content was very close, (r = .99***for the clover-grass mixture and couch grass and RSD’s of 0.6 and 0.5 respectively). At the early stages of maturity when the plant protein content and in vitro DM digestibility were high, the cellulase technique digested more efficiently the clover-grass mixture than the couch grass. The cellulase method was rapid, reproduciple and well suited to the evaluation of the plant material studied.

EFFECTS OF SELECTED REED CANARY GRASS ALKALOIDS ON IN VITRO DIGESTIBILITY

1977 ◽  
Vol 57 (3) ◽  
pp. 779-785 ◽  
Author(s):  
B. E. COULMAN ◽  
K. W. CLARK ◽  
D. L. WOODS
Keyword(s):  
Rumen Fluid ◽  
In Vitro Digestion ◽  
In Vitro Digestibility ◽  
Reed Canary Grass ◽  
Powdered Cellulose ◽  
Similar Work ◽  
Wide Range ◽  
Canary Grass ◽  
Rumen Microflora

A range of concentrations of pure samples of the alkaloids, gramine, hordenine, 5-methoxy-dimethyl tryptamine and N-methyl tryptamine which are normal constituents of reed canary grass were added to in vitro rumen fluid fermentations. Powdered cellulose and ground reed canary grass were used as substrates and fermentation periods were 24 and 48 h. No consistent relationship was found between the concentration of any of the above alkaloids and in vitro digestion values for either of the substrates or fermentation periods. In other studies, 24- and 48-h in vitro digestions were carried out on reed canary grass clones harvested at early heading and 6-wk regrowth. These clones showed a very wide range of gramine, hordenine and total alkaloid levels. No relationship was found between the concentration and type of alkaloids in the clones and their in vitro digestibility. These findings are in keeping with earlier similar work and it was concluded that reported poor animal performance with reed canary grass is probably due to factors other than alkaloid interference with the rumen microflora.

scholarly journals 127 Porcine in vitro digestibility of whole stillage predigested with multi-enzyme during different time periods

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 49-50
Author(s):  
Kevin S Jerez Bogota ◽  
Tofuko A Woyengo
Keyword(s):  
Dry Matter ◽  
In Vitro Digestion ◽  
Enzyme Treatment ◽  
Optimal Time ◽  
In Vitro Digestibility ◽  
Percentage Points ◽  
Whole Stillage ◽  
Freeze Dried ◽  
Different Sources

Abstract A study was conducted to determine the effects of the period of predigesting whole stillage (WS; slurry material that is dried into DDGS) with multi-enzyme and composition of the multi-enzyme on porcine in vitro digestibility of dry matter (IVDDM) of the WS. Four samples of whole stillage from 4 different sources were freeze-dried and divided into 13 subsamples to give 52 sub-samples. Thirteen treatments were applied to the 48 sub-samples within source. The treatments were undigested WS (control); or pre-digested with 1 of 3 multi-enzymes (MTE1, MTE2, and MTE3) at 55 °C for 6, 12, 18 or 24 h in 3 × 4 factorial arrangement. The MTE1 contained xylanase, β-glucanase, cellulase, mannanase, protease, and amylase; MTE2 contained xylanase, α-galactosidase, and cellulase; and MTE3 contained xylanase, cellulase, β-glucanase, and mannanase. The 52 subsamples were subjected to porcine in vitro digestion. The IVDDM of untreated WS was 73.3%. The IVDDM increased (P&lt; 0.05) with an increase in the predigestion period. However, a rise in the predigestion period from 0 to 12 h resulted in greater (P&lt; 0.05) response in mean IVDDM than an increment in the predigestion period from 12 to 24 h (11 vs. 0.83 percentage points). Predigestion period and multi-enzyme type interacted on IVDDM such that the improvement in IVDDM between 0 and 12 hours of predigestion differed (P&lt; 0.05) among the 3 multi-enzyme types (13.3, 11.1, and 8.5 percentage points for MTE3, MTE2, and MTE1, respectively). The LS means by multi-enzyme treatment were modeled and resulted in unparallel curves (P&lt; 0.05). The estimated maximum response of IVDDM for MTE1, MTE2 and MTE 3 were 82.4%, 84.7% and 87.1% at 15.8, 13 and 13.1 hours, respectively. In conclusion, the optimal time of predigestion of WS with multi-enzymes (with regard to improvement in its IVDDM) was approximately 14 h.

scholarly journals Metagenomic analysis of the cow, sheep, reindeer and red deer rumen

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Laura Glendinning ◽  
Buğra Genç ◽  
R. John Wallace ◽  
Mick Watson
Keyword(s):  
Cervus Elaphus ◽  
Rangifer Tarandus ◽  
Red Deer ◽  
Bos Taurus ◽  
Novel Species ◽  
Ovis Aries ◽  
Metagenomic Analysis ◽  
Reference Dataset ◽  
Microbial Genomes ◽  
Ruminant Species

AbstractThe rumen microbiota comprises a community of microorganisms which specialise in the degradation of complex carbohydrates from plant-based feed. These microbes play a highly important role in ruminant nutrition and could also act as sources of industrially useful enzymes. In this study, we performed a metagenomic analysis of samples taken from the ruminal contents of cow (Bos Taurus), sheep (Ovis aries), reindeer (Rangifer tarandus) and red deer (Cervus elaphus). We constructed 391 metagenome-assembled genomes originating from 16 microbial phyla. We compared our genomes to other publically available microbial genomes and found that they contained 279 novel species. We also found significant differences between the microbiota of different ruminant species in terms of the abundance of microbial taxonomies, carbohydrate-active enzyme genes and KEGG orthologs. We present a dataset of rumen-derived genomes which in combination with other publicly-available rumen genomes can be used as a reference dataset in future metagenomic studies.

In-Vitro production of wapiti and red deer (Cervus elaphus) embryos

1995 ◽  
Vol 43 (1) ◽  
pp. 301 ◽  
Author(s):  
J.W. Pollard ◽  
M.J. Bringans ◽  
B. Buckrell
Keyword(s):  
Cervus Elaphus ◽  
Red Deer ◽  
In Vitro Production ◽  
Vitro Production

Relationship between intake of silage and its chemical composition and in vitro digestibility

1972 ◽  
Vol 23 (1) ◽  
pp. 25 ◽  
Author(s):  
DC Brown ◽  
JC Radcliffe
Keyword(s):  
Dry Matter ◽  
In Vitro Digestion ◽  
Ammonia Nitrogen ◽  
Matter Content ◽  
In Vitro Digestibility ◽  
Dry Matter Content ◽  
Regression Analyses ◽  
Lactic Acids

Twenty experimental silages were made from seven pasture species at different stages of maturity. In vivo dry matter, organic matter, and energy ad libitum intakes and digestibilities of the silages were determined with standardized pairs of Merino wethers. The following chemical characteristics of the silages were measured: nitrogen, ammonia nitrogen, total titratable acids, acetic, propionic, butyric, and lactic acids, total volatiles lost during oven drying, lactic acid as a percentage of the total organic acids, pH, acid pepsin dry matter disappearance, dry matter content, and in vitro digestibility and rate of digestion. When all 20 silages were considered, energy intakes on a body weight basis were significantly related to silage pH (r = 0.55) and rate of in vitro digestion (r = 0.58). When the five legume silages were removed from the analysis and only the 15 grass-dominant silages were considered, dry matter intakes were significantly related to acetic (r = –0.57) and propionic acid (r = –0.55) concentrations. Multiple regression analyses did not significantly increase the accuracy of predicting intake. The results suggested that silage intake was negatively related to the degree of fermentation that occurred during the ensiling process.

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