Conservation genetics of the wood turtle (Glyptemys insculpta) in Quebec, Canada
Throughout its range, the long-term persistence of the wood turtle (Glyptemys insculpta Le Conte, 1830) is jeopardized by habitat perturbations and commercial collection. The main objective of this study was to acquire knowledge on the genetic structure of wood turtle populations within Quebec, where the species reaches the northernmost limit of its range, to identify proper conservation units. The six known populations in Quebec were genetically characterized using five microsatellite loci. Genetic analyses revealed high variability among all populations (HO ranging from 0.561 to 0.886), suggesting that past population declines have not yet affected their genetic diversity. Since populations are located on both shores of the St. Lawrence River, two colonization hypotheses were tested to determine whether the River acted as a dispersal route or as a barrier. Results of AMOVA tests, phylogenetic analyses, and assignment tests demonstrated the clear distinction between populations from both shores, and three genetically different units were identified: the two populations on the north shore and a single homogenous group on the south shore.