REAL TIME STUDIES OF ACETYLCHOLINE RELEASE FROM SINGLE SYNAPSES AND SINGLE CELLS WITH NANOMETER SPATIAL RESOLUTION

Author(s):  
Mei Shen ◽  
Ran Chen
2019 ◽  
Vol 11 (3) ◽  
pp. 327 ◽  
Author(s):  
Xia Wang ◽  
Feng Ling ◽  
Huaiying Yao ◽  
Yaolin Liu ◽  
Shuna Xu

Mapping land surface water bodies from satellite images is superior to conventional in situ measurements. With the mission of long-term and high-frequency water quality monitoring, the launch of the Ocean and Land Colour Instrument (OLCI) onboard Sentinel-3A and Sentinel-3B provides the best possible approach for near real-time land surface water body mapping. Sentinel-3 OLCI contains 21 bands ranging from visible to near-infrared, but the spatial resolution is limited to 300 m, which may include lots of mixed pixels around the boundaries. Sub-pixel mapping (SPM) provides a good solution for the mixed pixel problem in water body mapping. In this paper, an unsupervised sub-pixel water body mapping (USWBM) method was proposed particularly for the Sentinel-3 OLCI image, and it aims to produce a finer spatial resolution (e.g., 30 m) water body map from the multispectral image. Instead of using the fraction maps of water/non-water or multispectral images combined with endmembers of water/non-water classes as input, USWBM directly uses the spectral water index images of the Normalized Difference Water Index (NDWI) extracted from the Sentinel-3 OLCI image as input and produces a water body map at the target finer spatial resolution. Without the collection of endmembers, USWBM accomplished the unsupervised process by developing a multi-scale spatial dependence based on an unsupervised sub-pixel Fuzzy C-means (FCM) clustering algorithm. In both validations in the Tibet Plate lake and Poyang lake, USWBM produced more accurate water body maps than the other pixel and sub-pixel based water body mapping methods. The proposed USWBM, therefore, has great potential to support near real-time sub-pixel water body mapping with the Sentinel-3 OLCI image.


2020 ◽  
Vol 51 (3) ◽  
pp. 359-373
Author(s):  
Yu-Ting Qi ◽  
Fu-Li Zhang ◽  
Si-Yu Tian ◽  
Xiao-Ke Yang ◽  
Yan-Ling Liu ◽  
...  

Author(s):  
Kenneth H. Hu ◽  
John P. Eichorst ◽  
Chris S. McGinnis ◽  
David M. Patterson ◽  
Eric D. Chow ◽  
...  

ABSTRACTSpatial transcriptomics seeks to integrate single-cell transcriptomic data within the 3-dimensional space of multicellular biology. Current methods use glass substrates pre-seeded with matrices of barcodes or fluorescence hybridization of a limited number of probes. We developed an alternative approach, called ‘ZipSeq’, that uses patterned illumination and photocaged oligonucleotides to serially print barcodes (Zipcodes) onto live cells within intact tissues, in real-time and with on-the-fly selection of patterns. Using ZipSeq, we mapped gene expression in three settings: in-vitro wound healing, live lymph node sections and in a live tumor microenvironment (TME). In all cases, we discovered new gene expression patterns associated with histological structures. In the TME, this demonstrated a trajectory of myeloid and T cell differentiation, from periphery inward. A variation of ZipSeq efficiently scales to the level of single cells, providing a pathway for complete mapping of live tissues, subsequent to real-time imaging or perturbation.


2018 ◽  
Author(s):  
Julia Falo-Sanjuan ◽  
Nicholas C Lammers ◽  
Hernan G Garcia ◽  
Sarah Bray

SummaryInformation from developmental signaling pathways must be accurately decoded to generate transcriptional outcomes. In the case of Notch, the intracellular domain (NICD) transduces the signal directly to the nucleus. How enhancers decipher NICD in the real time of developmental decisions is not known. Using the MS2/MCP system to visualize nascent transcripts in single cells in Drosophila embryos we reveal how two target enhancers read Notch activity to produce synchronized and sustained profiles of transcription. By manipulating the levels of NICD and altering specific motifs within the enhancers we uncover two key principles. First, increased NICD levels alter transcription by increasing duration rather than frequency of transcriptional bursts. Second, priming of enhancers by tissue-specific transcription factors is required for NICD to confer synchronized and sustained activity; in their absence, transcription is stochastic and bursty. The dynamic response of an individual enhancer to NICD thus differs depending on the cellular context.


1976 ◽  
Vol 24 (1) ◽  
pp. 112-121 ◽  
Author(s):  
A S Farrow ◽  
J H Tucker

Run coding applied to the digitized video signal from a TV scan of cell preparations can effect a substantial reduction in the total amount of data, sufficient to permit a moderate size of store to be loaded within one frame time with a representation of the field adequate for computer analysis. This paper describes the design of a run coding interface between a TV scanner and a computer store which also allows control of scan domain, spatial resolution and density resolution. Results are presented showing its efficiency when dealing with cervical smear preparations.


2018 ◽  
Vol 115 (28) ◽  
pp. E6516-E6525 ◽  
Author(s):  
Stephan Uphoff

Evolutionary processes are driven by diverse molecular mechanisms that act in the creation and prevention of mutations. It remains unclear how these mechanisms are regulated because limitations of existing mutation assays have precluded measuring how mutation rates vary over time in single cells. Toward this goal, I detected nascent DNA mismatches as a proxy for mutagenesis and simultaneously followed gene expression dynamics in single Escherichia coli cells using microfluidics. This general microscopy-based approach revealed the real-time dynamics of mutagenesis in response to DNA alkylation damage and antibiotic treatments. It also enabled relating the creation of DNA mismatches to the chronology of the underlying molecular processes. By avoiding population averaging, I discovered cell-to-cell variation in mutagenesis that correlated with heterogeneity in the expression of alternative responses to DNA damage. Pulses of mutagenesis are shown to arise from transient DNA repair deficiency. Constitutive expression of DNA repair pathways and induction of damage tolerance by the SOS response compensate for delays in the activation of inducible DNA repair mechanisms, together providing robustness against the toxic and mutagenic effects of DNA alkylation damage.


The Analyst ◽  
2019 ◽  
Vol 144 (10) ◽  
pp. 3226-3238 ◽  
Author(s):  
Jitraporn Vongsvivut ◽  
David Pérez-Guaita ◽  
Bayden R. Wood ◽  
Philip Heraud ◽  
Karina Khambatta ◽  
...  

Coupling synchrotron IR beam to an ATR element enhances spatial resolution suited for high-resolution single cell analysis in biology, medicine and environmental science.


Agronomy ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 1674
Author(s):  
Ponraj Arumugam ◽  
Abel Chemura ◽  
Bernhard Schauberger ◽  
Christoph Gornott

Immediate yield loss information is required to trigger crop insurance payouts, which are important to secure agricultural income stability for millions of smallholder farmers. Techniques for monitoring crop growth in real-time and at 5 km spatial resolution may also aid in designing price interventions or storage strategies for domestic production. In India, the current government-backed PMFBY (Pradhan Mantri Fasal Bima Yojana) insurance scheme is seeking such technologies to enable cost-efficient insurance premiums for Indian farmers. In this study, we used the Decision Support System for Agrotechnology Transfer (DSSAT) to estimate yield and yield anomalies at 5 km spatial resolution for Kharif rice (Oryza sativa L.) over India between 2001 and 2017. We calibrated the model using publicly available data: namely, gridded weather data, nutrient applications, sowing dates, crop mask, irrigation information, and genetic coefficients of staple varieties. The model performance over the model calibration years (2001–2015) was exceptionally good, with 13 of 15 years achieving more than 0.7 correlation coefficient (r), and more than half of the years with above 0.75 correlation with observed yields. Around 52% (67%) of the districts obtained a relative Root Mean Square Error (rRMSE) of less than 20% (25%) after calibration in the major rice-growing districts (>25% area under cultivation). An out-of-sample validation of the calibrated model in Kharif seasons 2016 and 2017 resulted in differences between state-wise observed and simulated yield anomalies from –16% to 20%. Overall, the good ability of the model in the simulations of rice yield indicates that the model is applicable in selected states of India, and its outputs are useful as a yield loss assessment index for the crop insurance scheme PMFBY.


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