INVESTIGATION OF EFFECTS OF PORCINE CIRCOVIRUS TYPE 2 (PCV2) VACCINATION ON CELLULAR IMMUNITY IN PIGS IN A TAIWAN PIG FARM

2021 ◽  
pp. 1-9
Author(s):  
Yen-Chen Chang ◽  
Hui-Wen Chang ◽  
Hue-Ying Chiou ◽  
Chian-Ren Jeng ◽  
Victor Fei Pang ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Porcine Circovirus ◽  
Economic Losses ◽  
Con A ◽  
Expression Levels ◽  
Viral Loads ◽  
Mrna Expression Levels

Porcine circovirus type 2 (PCV2) is the major cause of serious porcine circovirus-associated diseases (PCVADs) causing significant economic losses in the pig industry. The PCV2 vaccines have proved effective by lowering serum and tissue viral loads. However, little is known about its effects on the cellular functions of viral target cells in pigs. For this, blood samples from 38 vaccinated and 34 non-vaccinated, 12–14 week-old, clinically healthy and PCV2-infected pigs in a commercial farm were sampled and anticoagulated. The viral load in plasma and the cellular proliferation and mRNA expression levels of IL-1 [Formula: see text], IL-2, IL-4, IL-8, IL-10 and IFN-[Formula: see text] of peripheral blood mononuclear cells (PBMCs) with or without concanavalin A (Con A) and PCV2 stimulation in vitro were then investigated by real-time polymerase chain reaction and flow cytometry. Compared with the non-vaccinated pigs, the average viral loads in the plasma and cultivated PBMCs were reduced in the PCV2-vaccinated pigs. Significant differences in the aspects of increased cell proliferation, decreased average viral loads in PBMCs three days after Con A stimulation, increased mRNA expression levels of IL-2 and IFN-[Formula: see text] after re-exposure to PCV2, and decreased levels of IL-1 [Formula: see text], IL-4 and IL-8 after Con A or PCV2 stimulation were also noted in the PCV2-vaccinated pigs. The results suggested that the PCV2-vaccinated pigs were likely to develop better cell-mediated immunity than the non-vaccinated pigs.

scholarly journals Whole-Genome Analysis of Porcine Circovirus Type 2 in Russia

Pathogens ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 1631
Author(s):  
Sergei Raev ◽  
Anton Yuzhakov ◽  
Taras Aliper
Keyword(s):  
Genetic Diversity ◽  
Genome Analysis ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Porcine Circovirus ◽  
Economic Losses ◽  
Whole Genome ◽  
Whole Genome Analysis ◽  
Reading Frame

Porcine circovirus type 2 (PCV2) is the causative agent of porcine circovirus-associated diseases (PCVAD) that bring about significant economic losses in the pig industry all over the world. The aim of this study was to investigate the genetic diversity of PCV2 in Russia and characterize the available complete genome sequences. PCV2 DNA was detected at all investigated farms located in different regions of Russia. Whole-genome analysis demonstrated that the majority of PCV2 strains belonged to genotype PCV2d (12 out of 14), while PCV2a and PCV2b were only detected at 2 farms (one at each). Further analysis revealed that all antibody recognition sites in Russian PCV2 strains were different from the corresponding epitopes in a PCV2a vaccine strain, suggesting that PCV2a-based vaccines may only provide limited protection against these strains. PCV2d strains could be grouped into 3 distinct lines which shared 98.7–100% identity within open reading frame 2 (ORF2). It is the first study reporting the genetic diversity of PCV2 strains in Russia. Our data indicated that, similarly to China, Europe, and USA, PCV2a and PCV2b have largely been replaced by PCV2d.

scholarly journals Macrophage Polarization Modulated by Porcine Circovirus Type 2 Facilitates Bacterial Coinfection

2021 ◽  
Vol 12 ◽  
Author(s):  
Wen Zhang ◽  
Zhendong Fu ◽  
Hongyan Yin ◽  
Qingbing Han ◽  
Wenhui Fan ◽  
...  
Keyword(s):  
Macrophage Polarization ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Pcv2 Infection ◽  
Porcine Circovirus ◽  
Economic Losses ◽  
Target Cells ◽  
M2 Macrophage ◽  
Bacterial Coinfection

Polarization of macrophages to different functional states is important for mounting responses against pathogen infections. Macrophages are the major target cells of porcine circovirus type 2 (PCV2), which is the primary causative agent of porcine circovirus–associated disease (PCVAD) leading to immense economic losses in the global swine industry. Clinically, PCV2 is often found to increase risk of other pathogenic infections yet the underlying mechanisms remain to be elusive. Here we found that PCV2 infection skewed macrophages toward a M1 status through reprogramming expression of a subset of M1-associated genes and M2-associated genes. Mechanistically, induction of M1-associated genes by PCV2 infection is dependent on activation of nuclear factor kappa B (NF-κB) and c-jun N-terminal kinase (JNK) signaling pathways whereas suppression of M2-associated genes by PCV2 is via inhibiting expression of jumonji domain containing-3 (JMJD3), a histone 3 Lys27 (H3K27) demethylase that regulates M2 activation of macrophages. Finally, we identified that PCV2 capsid protein (Cap) directly inhibits JMJD3 transcription to restrain expression of interferon regulatory factor (IRF4) that controls M2 macrophage polarization. Consequently, sustained infection of PCV2 facilitates bacterial infection in vitro. In summary, these findings showed that PCV2 infection functionally modulated M1 macrophage polarization via targeting canonical signals and epigenetic histone modification, which contributes to bacterial coinfection and virial pathogenesis.

scholarly journals Genomic Rearrangement and Recombination of Porcine Circovirus Type 2 and Porcine Circovirus-Like Virus P1 in China

2021 ◽  
Vol 8 ◽  
Author(s):  
Libin Wen ◽  
Kongwang He
Keyword(s):  
Genome Rearrangement ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Porcine Circovirus ◽  
Economic Losses ◽  
Protein Coding ◽  
Generic Term

Porcine circovirus type 2 (PCV2) belongs to the genus Circovirus of the family Circoviridae, and it has been associated with porcine circovirus (associated) disease (PCVD or PCVAD) in pigs. PCVAD is the generic term for a series of disease syndromes that have caused economic losses to the pig industry worldwide. Since the discovery of PCV2 in the late 1990s, the virus has continued to evolve, and novel genotypes have continued to appear. Moreover, there has been recombination between different genotypes of PCV2. This review attempts to illustrate some progress concerning PCV2 in genome rearrangement and genomic recombination with non-PCV2-related nucleic acids, particularly focusing on the porcine circovirus-like virus P1 formed by the recombination of PCV2. The presence of rearranged PCV2 genomes can be demonstrated both in vivo and in vitro, and these subviral molecules ranged from 358 to 1,136 bp. Depending on whether it has the ability to encode a protein, the agents formed by PCV2 recombination can be divided into two categories: porcine circovirus-like viruses and porcine circovirus-like mini agents. We mainly discuss the porcine circovirus-like virus P1 regarding genomic characterization, etiology, epidemiology, and pathogenesis. Further research needs to be conducted on the pathogenicity of other porcine circovirus-like viruses and porcine circovirus-like mini agents and the effects of their interactions with PCV2, especially for the porcine circovirus-like mini agents that do not have protein-coding functions in the genome.

scholarly journals Real-Time PCR Detection Patterns of Porcine Circovirus Type 2 (PCV2) in Polish Farms with Different Statuses of Vaccination against PCV2

Viruses ◽  
2019 ◽  
Vol 11 (12) ◽  
pp. 1135 ◽  
Author(s):  
Aleksandra Woźniak ◽  
Dagmara Miłek ◽  
Piotr Matyba ◽  
Tomasz Stadejek
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Porcine Circovirus ◽  
Quantitative Real Time Pcr ◽  
Viral Loads ◽  
Oral Fluids ◽  
Significant Difference

Porcine circovirus type 2 (PCV2) is a globally spread pathogen controlled with generally highly efficacious vaccination protocols. In order to compare PCV2 detection profiles in farms with different vaccination statuses, serum (359) and fecal pools (351) and oral fluids (209) from four farms that do not vaccinate against PCV2 (NON-VAC) and from 22 farms that do vaccinate (VAC) were tested with quantitative real-time PCR. Additionally, nucleotide sequences of ORF2 of the virus were obtained from selected samples. Three genotypes, PCV2a, PCV2b, and PCV2d, were detected. Significant differences (p < 0.05) in PCV2 prevalence and quantities between the VAC and NON-VAC farms were evident. In five VAC farms, no viremia or shedding in feces was detected. On the other hand, in four VAC farms, the results were very similar to those from NON-VAC farms. No significant difference in PCV2 prevalence in oral fluids was observed between VAC and NON-VAC farms. An examination of viremia can be recommended for the detection of vaccination efficacy issues. The median of the PCV2 viral loads >6.0 log10 copies/mL in pooled sera from the vaccinated population should be considered a very strong indication that the vaccination protocol needs revision.

scholarly journals Combined Effect of Deoxynivalenol (DON) and Porcine Circovirus Type 2 (Pcv2) on Inflammatory Cytokine mRNA Expression

Toxins ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 422
Author(s):  
Chao Gu ◽  
Xiuge Gao ◽  
Dawei Guo ◽  
Jiacai Wang ◽  
Qinghua Wu ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Signaling Pathway ◽  
Porcine Circovirus Type ◽  
Mapk Signaling ◽  
Porcine Circovirus Type 2 ◽  
Mapk Signaling Pathway ◽  
Porcine Circovirus ◽  
Signal Pathways ◽  
Dependent Manner

A host’s immune system can be invaded by mycotoxin deoxynivalenol (DON) poisoning and porcine circovirus type 2 (PCV2) infections, which affect the host’s natural immune function. Pro-inflammatory cytokines, IL-1β and IL-6, are important regulators in the process of natural immune response, which participate in inflammatory response and enhance immune-mediated tissue damage. Preliminary studies have shown that DON promotes PCV2 infection by activating the MAPK signaling pathway. Here, we explored whether the mRNA expression of IL-1β and IL-6, induced by the combination of DON and PCV2, would depend on the MAPK signaling pathway. Specific pharmacological antagonists U0126, SP600125 and SB203580, were used to inhibit the activities of ERK, JNK and p38 in the MAPK signaling pathway, respectively. Then, the mRNA expression of IL-1β and IL-6 in PK-15 cells was detected to explore the effect of the MAPK signaling pathway on IL-1β and IL-6 mRNA induced by DON and PCV2. The results showed that PK-15 cells treated with DON or PCV2 induced the mRNA expression of IL-1β and IL-6 in a time- and dose-dependent manner. The combination of DON and PCV2 has an additive effect on inducing the mRNA expression of IL-1β and IL-6. Additionally, both DON and PCV2 could induce the mRNA expression of IL-1β and IL-6 via the ERK and the p38 MAPK signal pathways, while PCV2 could induce it via the JNK signal pathway. Taken together, our results suggest that MAPKs play a contributory role in IL-1β and IL-6 mRNA expression when induced by both DON and PCV2.

scholarly journals DETECTION OF PORCINE CIRCOVIRUS TYPE 3 IN RUSSIAN PIG HOLDINGS

2019 ◽  
pp. 29-33
Author(s):  
M. V. Biryuchenkova ◽  
A. M. Timina ◽  
A. V. Shcherbakov
Keyword(s):  
Russian Federation ◽  
Clinical Manifestations ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Porcine Circovirus ◽  
Economic Losses ◽  
Unknown Etiology ◽  
The Russian Federation ◽  
Type 3

Diseases associated with porcine circoviruses (mainly with porcine circovirus type 2) have various manifestations, are common in pigs in countries having well-developed pig industry and responsible for significant economic losses. Porcine circovirus type 3 (PCV-3) causing systemic inflammation of unknown etiology in animals was detected the USA in 2015. Later, data on PCV-3 detection in Asia, Europe and South America were published. Analysis of literature data on current epidemic situation on PCV-3 infection in foreign countries as well as the disease clinical manifestations and postmortem lesions are described. Results of molecular and genetic tests of biomaterials collected from pigs in 51 holdings located in 28 regions of the Russian Federation are presented. A total of 280 samples of biological materials of different types (organs, tissues, stillborn piglets) collected from domestic pigs with respiratory, reproductive and neurological disorders, dermatitis and from emaciated pigs were tested and PCV-3 genome was detected in 11 samples from 9 holdings located in 5 regions of the Russian Federation. Porcine circovirus type 3 was detected in lung, bronchial and mediastinal lymph node, spleen tissues from grower and fattening piglets, adult pigs and aborted fetuses. Samples that were positive for PCV-3 DNA when tested with molecular methods (PCR, real-time PCR) were tested for other pathogens. The following pathogens were also detected in 6 out of 11 samples (55%): Actinobacillus pleuropneumoniae, Mycoplasma hyorhinis, Streptococcus suis, Haemophilus parasuis, Mycoplasma hyopneumoniae and Pasteurella multocida. Porcine circovirus type 2 was detected in one sample. Presented test results are indicative of probable combined etiology of respiratory and reproductive disorders in tested pigs that results in various clinical manifestations. Grower and fattening piglets were found to be the most susceptible to PCV-3-associated disease. Further studies are required for identification of actual PCV-3 pathogenicity and its prevalence in the territory of the Russian Federation.

scholarly journals Genetic Diversity and Prevalence of Porcine Circovirus Type 2 in China During 2000-2019

2021 ◽  
Vol 8 ◽  
Author(s):  
Ning Li ◽  
Jing Liu ◽  
Jiali Qi ◽  
Feng Hao ◽  
Lei Xu ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Wide Distribution ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Amino Acid Sequences ◽  
Porcine Circovirus ◽  
Economic Losses ◽  
Swine Industry ◽  
Emerging Virus

As the major pathogen for porcine circovirus-associated disease (PCVAD), porcine circovirus type 2 (PCV2) is no longer treated as an emerging virus anymore. The wide distribution of PCV2 infection in China causes huge economic losses in the swine industry. Currently, it is generally believed that PCV2 has eight genotypes (PCV2a to PCV2h), with PCV2a, PCV2b, and PCV2d being widely distributed. To comprehensively explore the genetic diversity and prevalence of PCV2 in China, PCV-2 sequences submitted from China in the GenBank database were retrieved. With a total of 714 PCV2 strains were retrieved, we found that early-submitted PCV2 sequences were mainly collected from coastal provinces in the southeast part of China, which may indicate PCV2 was initially circulating in those regions. From 2002 to 2008, PCV2b was the dominant prevalent genotype in those retrieved sequences. From 2009, PCV2d became the dominant genotype in those sequences, dropping a hint that a potential shift of PCV2b to PCV2d might occur in 2009, which is similar to the patterns at the global level. In addition to the PCV2a, PCV2b, and PCV2d genotypes, novel strains were also characterized. We further revealed that the amino acid sequences consistency of PCV2a Cap is higher than those in other genotypes. Together, this study provided clues for the possible prevalent genotypes and dynamics of genetic diversity in China from 2000 to 2019.

scholarly journals A Slaughterhouse Survey for Porcine Circovirus Type 2 in Commercial Pigs in Ibadan, Southwest Nigeria

2018 ◽  
Vol 62 (2) ◽  
pp. 30-34 ◽  
Author(s):  
C. O. Aiki-Raji ◽  
A. I. Adebiyi ◽  
D. O. Oluwayelu
Keyword(s):  
Control Strategies ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Porcine Circovirus ◽  
Economic Losses ◽  
Carrier Status ◽  
Swine Industry ◽  
Southwest Nigeria ◽  
Swine Disease

Abstract Porcine circovirus type 2 (PCV2) is recognized as one of the most important agents of reproductive disorders in gilts and sows worldwide. It is associated with considerable economic losses in the swine industry due to the unthriftiness, and variable morbidity and mortality it causes in pigs. In spite of the devastation caused by this virus to the global pig industry, there is little or no report of its occurrence in Nigeria. Hence, a slaughterhouse based survey was conducted to determine the prevalence of PCV2 infections in pigs in Ibadan, southwest Nigeria. Using a commercial ELISA kit, 364 pig sera collected from a major abattoir were screened for IgG antibodies against PCV2. The overall prevalence of anti-PCV2 antibodies in the pigs was 1.4 % (5/364), with more female pigs (4/237, 1.7 %) being seropositive than males (1/127, 0.8 %). Since there is no routine vaccination against this swine disease in Nigeria, thus the antibodies detected in the pig sera indicated a natural exposure to the virus. The absence of clinical disease in the pigs also suggests the possibility of a carrier status for these animals and shows that they could serve as hosts for the perpetuation of the disease. These findings underscore the need for continuous surveillance for PCV2 among pigs in Nigeria in order to determine its contribution to production losses incurred in the Nigerian swine industry and aid the development of prevention and control strategies against the disease.

Effect of porcine circovirus type 2 (PCV2) infection on reproduction: disease, vertical transmission, diagnostics and vaccination

2011 ◽  
Vol 12 (1) ◽  
pp. 47-65 ◽  
Author(s):  
D. M. Madson ◽  
T. Opriessnig
Keyword(s):  
Vertical Transmission ◽  
Current Knowledge ◽  
Clinical Signs ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Growing Pigs ◽  
Pcv2 Infection ◽  
Porcine Circovirus ◽  
Economic Losses

AbstractPorcine circovirus type 2 (PCV2) causes great economic losses in growing pigs and there are several reviews on disease manifestations and lesions associated with PCV2 in growing pigs. Reproductive failure in breeding herds, predominately associated with increased numbers of mummies and non-viable piglets at parturition, is one of the disease manifestations of PCV2 infection. Boars shed low amounts of infectious PCV2 in semen for extended time periods, and vertical transmission of PCV2 to fetuses during PCV2 viremia of the dam has been experimentally confirmed. However, intrauterine-infected piglets often are clinically normal. Nevertheless, pigs infected with PCV2 by the intrauterine route can be born viremic, possibly contributing to horizontal spread of PCV2 within the breeding herd and into the nursery. Shedding of PCV2 in semen and prevalence of intrauterine-infected piglets can both be greatly reduced by PCV2 vaccination well ahead of expected PCV2 exposure. This review is a discussion on current knowledge on the effects of PCV2 infection in the dam and inin uterofetuses, including clinical signs, lesions, diagnosis and prevention through vaccination. Infection of boars with PCV2, the potential for PCV2 transmission via semen and prevention of PCV2 shedding are also discussed.

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