DIABETIC INTERSTITIAL GLUCOSE IN THE SKIN TISSUE BY ATR-FTIR SPECTROSCOPY VERSUS CAPILLARY BLOOD GLUCOSE

2010 ◽  
Vol 03 (02) ◽  
pp. 81-90
Author(s):  
NATALJA SKREBOVA EIKJE
Keyword(s):  
Blood Glucose ◽  
Glucose Metabolism ◽  
Ftir Spectroscopy ◽  
Continuous Monitoring ◽  
Skin Tissue ◽  
Promising Alternative ◽  
Glucose Levels ◽  
Patients With Diabetes ◽  
Interstitial Glucose

Recently introduced horizontal attenuated total reflectance (HATR) Fourier transform infrared (FTIR) spectroscopy for real-time assessment and continuous monitoring of glucose biomolecules in the skin tissue directly on the patients might appear a promising alternative to interpret the activity of interstitial glucose metabolism in vivo by means of evaluating the dynamics of changes of glucose concentrations in interstitial fluid (IF). In the present study, in vivo spectra by ATR-FTIR spectroscopy were obtained post-prandially during a 120–180-minute continuous monitoring in three patients with type 2 diabetes and compared to pre-prandial spectra. In all patients with diabetes interstitial glucose levels at 1030 and 1041 cm-1 reflected the best relationship with blood glucose. The lag time (LT) required for glucose to diffuse from the capillary to epidermal skin tissue was calculated between 0 and 60 minutes at all measured glucose biomolecules. Data showed intra- and inter-subject variations of each glucose biomolecule, pointing to similarities and differences among interstitial glucose metabolism of the patients. Finally, the findings suggest that HATR-FTIR spectroscopy might have the potential for clinical interpretation of activity of glucose metabolism for diagnosis, management, and treatment of patients with diabetes.

Determination of Glucose Levels Using a Functionalized Hydrogel−Optical Fiber Biosensor: Toward Continuous Monitoring of Blood Glucose in Vivo

2009 ◽  
Vol 81 (9) ◽  
pp. 3630-3636 ◽  
Author(s):  
Sven Tierney ◽  
Berit M. Hasle Falch ◽  
Dag Roar Hjelme ◽  
Bjørn Torger Stokke
Keyword(s):  
Blood Glucose ◽  
Optical Fiber ◽  
Continuous Monitoring ◽  
Glucose Levels

Opuntia stricta Cladode Extract Reduces Blood Glucose Levels in Alloxan-induced Diabetic Mice

2020 ◽  
Author(s):  
Lungwani Muungo
Keyword(s):  
Blood Glucose ◽  
Aqueous Extract ◽  
Experimental Period ◽  
Diabetic Mice ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Patients With Diabetes ◽  
Alloxan Monohydrate ◽  
Antidiabetic Effects

Opuntia stricta (commonly called prickly pear cactus) is a natural plant that grows in some partsof Zambia where its fruits and cladodes are commonly consumed for nutritional and medicinal purposes, including glycaemiccontrol among some patients with diabetes mellitus (DM). There is insufficient evidence whether Opuntia strictaindigenously growing in Zambia possess antidiabetic effects. Aim: To assess in vivo antidiabetic effects of the aqueousextract of Opuntia stricta cladodes in alloxan-induced diabetic mice. Methods: A laboratory-based experimental study wasconducted involving 20 adult Swiss albino mice (Mus musculus) weighing 18-30 g. DM was induced using a singleintraperitoneal dose of alloxan monohydrate 90 mg/kg. Opuntia stricta aqueous extract was administered orally and bloodglucose levels (in mmol/L) monitored daily for 10 days. Results: Alloxan induced a 4- to 5-fold sustained increase in bloodglucose levels at 72 hours after administration in mice. Within a 10-day experimental period, Opuntia stricta cladode aqueousextract (1 mg/kg) significantly reduced blood glucose levels in vivo (from 16.6 ± 1.4 mmol/L, 95% CI: 14.9-18.3 at baselineto 7.5 ± 1.0 mmol/L, 95% CI: 6.2-8.9 at endpoint, p < 0.001, n = 5). Similarly, at a dose of 2 mg/kg, the extractsignificantly reduced blood glucose levels (from 18.7 ± 4.6 mmol/L, 95% CI: 13.0-24.4 at baseline to 6.9 ± 1.7 mmol/L,95% CI: 4.7-9.0 at endpoint, p = 0.001, n = 5). Opuntia stricta cladode aqueous extract attained a greater reduction in bloodglucose levels compared to Glibenclamide 0.25 mg/kg. Opuntia stricta cladode aqueous extract demonstrated a presence ofalkaloids, flavonoids, saponins, sterols, carbohydrates, phenols and tannins. Conclusion: Opuntia stricta cladode fromZambia demonstrates antidiabetic effects to reduce blood glucose levels in vivo.

In Vivo Near-Infrared Spectroscopy of Rat Skin Tissue with Varying Blood Glucose Levels

2006 ◽  
Vol 78 (1) ◽  
pp. 215-223 ◽  
Author(s):  
Jonathon T. Olesberg ◽  
Lingzhi Liu ◽  
Valerie Van Zee ◽  
Mark A. Arnold
Keyword(s):  
Infrared Spectroscopy ◽  
Blood Glucose ◽  
Near Infrared Spectroscopy ◽  
Near Infrared ◽  
Skin Tissue ◽  
Rat Skin ◽  
Glucose Levels ◽  
Blood Glucose Levels

Examining Sensor Agreement in Neural Network Blood Glucose Prediction

2021 ◽  
pp. 193229682110182
Author(s):  
Aaron P. Tucker ◽  
Arthur G. Erdman ◽  
Pamela J. Schreiner ◽  
Sisi Ma ◽  
Lisa S. Chow
Keyword(s):  
Neural Network ◽  
Blood Glucose ◽  
Patient Specific ◽  
Sensor Location ◽  
Effective Care ◽  
Freestyle Libre ◽  
Patients With Diabetes ◽  
Interstitial Glucose ◽  
Continuous Glucose Monitors ◽  
Glucose Prediction

Successful measurements of interstitial glucose are a key component in providing effective care for patients with diabetes. Recently, there has been significant interest in using neural networks to forecast future glucose values from interstitial measurements collected by continuous glucose monitors (CGMs). While prediction accuracy continues to improve, in this work we investigated the effect of physiological sensor location on neural network blood glucose forecasting. We used clinical data from patients with Type 2 Diabetes who wore blinded FreeStyle Libre Pro CGMs (Abbott) on both their right and left arms continuously for 12 weeks. We trained patient-specific prediction algorithms to test the effect of sensor location on neural network forecasting ( N = 13, Female = 6, Male = 7). In 10 of our 13 patients, we found at least one significant ( P < .05) increase in forecasting error in algorithms which were tested with data taken from a different location than data which was used for training. These reported results were independent from other noticeable physiological differences between subjects (eg, height, age, weight, blood pressure) and independent from overall variance in the data. From these results we observe that CGM location can play a consequential role in neural network glucose prediction.

scholarly journals Sam68 promotes hepatic gluconeogenesis via CRTC2

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Aijun Qiao ◽  
Junlan Zhou ◽  
Shiyue Xu ◽  
Wenxia Ma ◽  
Chan Boriboun ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Therapeutic Target ◽  
Rna Binding ◽  
Adaptor Protein ◽  
Diabetic Mouse ◽  
Mrna Levels ◽  
Hepatic Gluconeogenesis ◽  
Kinase Substrate ◽  
Glucose Levels ◽  
Patients With Diabetes

AbstractHepatic gluconeogenesis is essential for glucose homeostasis and also a therapeutic target for type 2 diabetes, but its mechanism is incompletely understood. Here, we report that Sam68, an RNA-binding adaptor protein and Src kinase substrate, is a novel regulator of hepatic gluconeogenesis. Both global and hepatic deletions of Sam68 significantly reduce blood glucose levels and the glucagon-induced expression of gluconeogenic genes. Protein, but not mRNA, levels of CRTC2, a crucial transcriptional regulator of gluconeogenesis, are >50% lower in Sam68-deficient hepatocytes than in wild-type hepatocytes. Sam68 interacts with CRTC2 and reduces CRTC2 ubiquitination. However, truncated mutants of Sam68 that lack the C- (Sam68ΔC) or N-terminal (Sam68ΔN) domains fails to bind CRTC2 or to stabilize CRTC2 protein, respectively, and transgenic Sam68ΔN mice recapitulate the blood-glucose and gluconeogenesis profile of Sam68-deficient mice. Hepatic Sam68 expression is also upregulated in patients with diabetes and in two diabetic mouse models, while hepatocyte-specific Sam68 deficiencies alleviate diabetic hyperglycemia and improves insulin sensitivity in mice. Thus, our results identify a role for Sam68 in hepatic gluconeogenesis, and Sam68 may represent a therapeutic target for diabetes.

scholarly journals Glycemic variability and cardiovascular disease in patients with type 2 diabetes

2021 ◽  
Vol 9 (1) ◽  
pp. e002032
Author(s):  
Marcela Martinez ◽  
Jimena Santamarina ◽  
Adrian Pavesi ◽  
Carla Musso ◽  
Guillermo E Umpierrez
Keyword(s):  
Type 2 Diabetes ◽  
Blood Glucose ◽  
Glycated Hemoglobin ◽  
Glucose Monitoring ◽  
Glycemic Variability ◽  
Cardiovascular Complications ◽  
Glucose Levels ◽  
Increased Risk ◽  
Patients With Diabetes

Glycated hemoglobin is currently the gold standard for assessment of long-term glycemic control and response to medical treatment in patients with diabetes. Glycated hemoglobin, however, does not address fluctuations in blood glucose. Glycemic variability (GV) refers to fluctuations in blood glucose levels. Recent clinical data indicate that GV is associated with increased risk of hypoglycemia, microvascular and macrovascular complications, and mortality in patients with diabetes, independently of glycated hemoglobin level. The use of continuous glucose monitoring devices has markedly improved the assessment of GV in clinical practice and facilitated the assessment of GV as well as hypoglycemia and hyperglycemia events in patients with diabetes. We review current concepts on the definition and assessment of GV and its association with cardiovascular complications in patients with type 2 diabetes.

Effects of MDMA on blood glucose levels and brain glucose metabolism

2007 ◽  
Vol 34 (6) ◽  
pp. 916-925 ◽  
Author(s):  
M. L. Soto-Montenegro ◽  
J. J. Vaquero ◽  
C. Arango ◽  
G. Ricaurte ◽  
P. García-Barreno ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Glucose Metabolism ◽  
Brain Glucose ◽  
Brain Glucose Metabolism ◽  
Glucose Levels ◽  
Blood Glucose Levels

scholarly journals PENGARUH JUS LIDAH BUAYA TERHADAP KADAR GLUKOSA DARAH PUASA DAN 2 JAM PP(Post Prandial) PADA PENDERITA DIABETES MELITUS

2019 ◽  
Vol 10 (1) ◽  
pp. 77
Author(s):  
Febria Syafyu Sari ◽  
Ridhyalla Afnuhazi
Keyword(s):  
Diabetes Mellitus ◽  
Blood Glucose ◽  
Blood Sugar ◽  
Fasting Blood Glucose ◽  
Aloe Vera ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Patients With Diabetes ◽  
Paired T Test ◽  
Diabetes Melitus

ABSTRAK Diabetes Melitus merupakan penyakit yang paling menonjol yang disebabkan oleh gagalnya pengaturan gula darah. Lidah buaya berkhasiat untuk menurunkan kadar gula dalam darah bagi penderita diabetes dan dapat mengontrol tekanan darah. Tujuan penelitian untuk mengetahui pengaruh jus lidah buaya (AloeBarbadensis Miller) terhadap penurunan glukosa darah puasa GDP) dan 2 Jam PP (Post Prendial) pada penderita DM (Diabetes Melitus). Desain penelitian merupakan Quasi Eksperimental dengan pendekatan one group pretest – postest design. Sampel terbagi menjadi 14 responden. Data dianalisis dengan paired t-test. Hasil menunjukan rata-rata penurunan glukosa darah puasa pada intervensi (28,42 gr/dl) dan glukosa darah 2 jam pp pada intervensi (40,57 gr/dl). Untuk analisis bivariat terdapat perbedaan antara glukosa puasa dan 2 jam pp dengan glukosa darah puasa GDP) dan 2 Jam PP (Post Prendial) pada penderita DM (Diabetes Melitus). Kesimpulan didapatkanlidah buaya dapat menurunkan kadar glukosa darah.Berdasarkan hasil penelitian jus lidah buaya dapat menjadi salah satu alternatif keperawatan non farmakologi dalam penyakit diabetes melitus. Kata Kunci : Lidah Buaya ; Diabetes Mellitus THE EFFECT OF VEGETABLE VOCATIONAL JUICE ON FAST BLOOD GLUCOSE LEVELS AND 2 HOURS OF PP (Post Prandial) IN DIABETES MELLITUS  ABSTRACT Diabetes mellitus is the most prominent disease caused by the failure of blood sugar regulation. Aloe vera is efficacious can to  reduce blood sugar levels for diabetics and can control blood pressure. The purpose of this study was to determine the effect of Aloe Barbadensis Miller on the reduction of fasting blood glucose GDP and 2 hours of PP (post prendial) in patients with diabetes mellitus. The research design is Experimental Quasi with one group pretest - postest design approach. The sample is divided into 14 respondents. Data were analyzed by paired t-test. The results showed an average decrease in fasting blood glucose at intervention (28.42 gr / dl) and 2 hours pp blood glucose at intervention (40.57 gr / dl). For bivariate analysis there was a difference between fasting glucose and 2 hours pp with fasting blood glucose GDP) and 2 hours PP (Post Prendial) in patients with diabetes mellitus. The conclusion is that aloe vera can reduce blood glucose levels. Based on the results of research on aloe vera juice can be an alternative non-pharmacological nursing in diabetes mellitus. Keywords: Aloe Vera ; Diabetes Mellitus

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