Opuntia stricta Cladode Extract Reduces Blood Glucose Levels in Alloxan-induced Diabetic Mice

2020 ◽  
Author(s):  
Lungwani Muungo
Keyword(s):  
Blood Glucose ◽  
Aqueous Extract ◽  
Experimental Period ◽  
Diabetic Mice ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Patients With Diabetes ◽  
Alloxan Monohydrate ◽  
Antidiabetic Effects

Opuntia stricta (commonly called prickly pear cactus) is a natural plant that grows in some partsof Zambia where its fruits and cladodes are commonly consumed for nutritional and medicinal purposes, including glycaemiccontrol among some patients with diabetes mellitus (DM). There is insufficient evidence whether Opuntia strictaindigenously growing in Zambia possess antidiabetic effects. Aim: To assess in vivo antidiabetic effects of the aqueousextract of Opuntia stricta cladodes in alloxan-induced diabetic mice. Methods: A laboratory-based experimental study wasconducted involving 20 adult Swiss albino mice (Mus musculus) weighing 18-30 g. DM was induced using a singleintraperitoneal dose of alloxan monohydrate 90 mg/kg. Opuntia stricta aqueous extract was administered orally and bloodglucose levels (in mmol/L) monitored daily for 10 days. Results: Alloxan induced a 4- to 5-fold sustained increase in bloodglucose levels at 72 hours after administration in mice. Within a 10-day experimental period, Opuntia stricta cladode aqueousextract (1 mg/kg) significantly reduced blood glucose levels in vivo (from 16.6 ± 1.4 mmol/L, 95% CI: 14.9-18.3 at baselineto 7.5 ± 1.0 mmol/L, 95% CI: 6.2-8.9 at endpoint, p < 0.001, n = 5). Similarly, at a dose of 2 mg/kg, the extractsignificantly reduced blood glucose levels (from 18.7 ± 4.6 mmol/L, 95% CI: 13.0-24.4 at baseline to 6.9 ± 1.7 mmol/L,95% CI: 4.7-9.0 at endpoint, p = 0.001, n = 5). Opuntia stricta cladode aqueous extract attained a greater reduction in bloodglucose levels compared to Glibenclamide 0.25 mg/kg. Opuntia stricta cladode aqueous extract demonstrated a presence ofalkaloids, flavonoids, saponins, sterols, carbohydrates, phenols and tannins. Conclusion: Opuntia stricta cladode fromZambia demonstrates antidiabetic effects to reduce blood glucose levels in vivo.

scholarly journals Wild Strawberry, Blackberry, and Blueberry Leaf Extracts Alleviate Starch-Induced Hyperglycemia in Prediabetic and Diabetic Mice

Planta Medica ◽  
2020 ◽  
Vol 86 (11) ◽  
pp. 790-799
Author(s):  
István Takács ◽  
András Szekeres ◽  
Ákos Takács ◽  
Dávid Rakk ◽  
Miklós Mézes ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Postprandial Hyperglycemia ◽  
Diabetic Mice ◽  
Leaf Extracts ◽  
Cardiac Muscle Cells ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Wild Strawberry

AbstractIntestinal α-glucosidase and α-amylase break down nutritional poly- and oligosaccharides to monosaccharides and their activity significantly contributes to postprandial hyperglycemia. Competitive inhibitors of these enzymes, such as acarbose, are effective antidiabetic drugs, but have unpleasant side effects. In our ethnopharmacology inspired investigations, we found that wild strawberry (Fragaria vesca), blackberry (Rubus fruticosus), and European blueberry (Vaccinium myrtillus) leaf extracts inhibit α-glucosidase and α-amylase enzyme activity in vitro and are effective in preventing postprandial hyperglycemia in vivo. Toxicology tests on H9c2 rat embryonic cardiac muscle cells demonstrated that berry leaf extracts have no cytotoxic effects. Oral administration of these leaf extracts alone or as a mixture to normal (control), obese, prediabetic, and streptozotocin-induced diabetic mice attenuated the starch-induced rise of blood glucose levels. The efficiency was similar to that of acarbose on blood glucose. These results highlight berry leaf extracts as candidates for testing in clinical trials in order to assess the clinical significance of their effects on glycemic control.

scholarly journals In Vitro and In Vivo Effects of Norathyriol and Mangiferin on α-Glucosidase

2017 ◽  
Vol 2017 ◽  
pp. 1-7 ◽  
Author(s):  
Zhi-Long Shi ◽  
Yi-Dan Liu ◽  
Yun-Yun Yuan ◽  
Da Song ◽  
Mei-Feng Qi ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Glucose Tolerance Test ◽  
Fasting Blood Glucose ◽  
Tolerance Test ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
In Vivo Effects ◽  
Antidiabetic Effects

Norathyriol is a metabolite of mangiferin. Mangiferin has been reported to inhibit α-glucosidase. To the best of our knowledge, no study has been conducted to determine or compare those two compounds on inhibiting α-glucosidase in vitro and in vivo by far. In this study, we determined the inhibitory activity of norathyriol and mangiferin on α-glucosidase in vitro and evaluated their antidiabetic effect in diabetic mice. The results showed that norathyriol inhibited α-glucosidase in a noncompetitive manner with an IC50 value of 3.12 μM, which is more potent than mangiferin (IC50 = 358.54 μM) and positive drug acarbose (IC50 = 479.2 μM) in the zymological experiment. Both of norathyriol and mangiferin caused significant (p<0.05) reduction in fasting blood glucose and the blood glucose levels at two hours after carbohydrate loading and it was interesting that mangiferin and norathyriol can make the decline of the blood glucose earlier than other groups ever including normal group in the starch tolerance test. However, norathyriol and mangiferin did not significantly influence carbohydrate absorption in the glucose tolerance test. Therefore, the antidiabetic effects of norathyriol and mangiferin might be associated with α-glucosidase, and norathyriol was more potent than mangiferin.

scholarly journals POTENSI EKSTRAK KULIT BATANG BUNI (Antidesma bunius L. Spreng) UNTUK MENURUNKAN KADAR GLUKOSA DARAH TIKUS WISTAR HIPERGLIKEMIA

Jurnal Kimia ◽  
2020 ◽  
pp. 5
Author(s):  
N. W. I. Paramitha ◽  
I. M. Sukadana ◽  
S. R. Santi
Keyword(s):  
Blood Glucose ◽  
Ethanol Extract ◽  
Stem Bark ◽  
Negative Control ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Reduce Blood Glucose ◽  
Alloxan Monohydrate

Buni (Antidesma bunius L. Spreng) is one of the traditional medicinal plants whose stem bark has been proven in vitro as an antidiabetic by inhibiting the activity of the ? glucosidase enzyme. This study aims to determine the potential of ethanol extract of buni bark to reduce blood glucose levels in wistar rats in vivo induced by alloxan. Two groups of control rats K (+) and K (-), and three groups of treated rats (P1, P2, and P3) which were given the extract with a dose of 50, 100, and 200 mg/ kg BW respectively, were induced with 150 mg / kg BW alloxan monohydrate to make the rats hyperglycemic. On the 14th day of the trial (posttest) the average blood glucose level was tested using ANOVA with a = 0.05 and Tamhane's. It was concluded that the 200 mg / kg BW ethanol extract of buni bark was able to reduce blood glucose levels to closer to the normal condition when compared to negative control K(-). Keywords: antidiabetic,  Antidesma bunius L. Spreng., GC-MS, stem barks

scholarly journals Induced Hyperglycemia in Mice is Controlled Following the Microfluidic System- Assisted Transplantation of Stem Cells-derived Insulin-producing Cells Transduced with miRNA

2020 ◽  
Author(s):  
Adele Soltani ◽  
Masoud Soleimani ◽  
Mohammad Adel Ghiass ◽  
Seyed Ehsan Enderami ◽  
Shahram Rabbani ◽  
...  
Keyword(s):  
Stem Cells ◽  
Blood Glucose ◽  
Functional Condition ◽  
Microfluidic System ◽  
Diabetic Mice ◽  
Differentiation Potential ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Insulin Producing Cells

Abstract BackgroundCell-based therapy is a promising approach for the treatment of type 1 diabetes mellitus. Identification of stem cells as progenitor stem cells with differentiation potential to Insulin-producing cells (IPCs) and their application is an emerging issue. Different strategies have been used to support the cell survival and their specific functions to control hyperglycemia condition. Novel technology systems using appropriate materials/fibres can improve the cell transplantation.MethodsIn the present study, glucose-sensitive insulin-producing cells (IPCs) were differentiated from adipose-derived stem cells (ADSCs) transduced with miR-375 and anti-miR-7 to enhance the functions of the cells. The survival rate of the cells was also improved by using a microfluidic system prior to in vivo transplantation of the IPCs. The contribution of miR-375 with the anti-miR-7 in mature IPCs derived from ADSCs resulted in gaining the function of the cells as judged by insulin productionResultsAfter adopting a stable functional condition of the IPCs, the cells were used for in vivo grafting to diabetic mice which resulted in a substantial drop (5-folds) in blood glucose during four weeks of grafting. The pattern of blood glucose levels in the mice receiving fiber entrapped IPCs was similar to that of non-diabetic mice and blood glucose declined in animals treated with fiber-entrapped-IPCs. Blood insulin was elevated (2-folds) in diabetic mice received transplant of fiber-entrapped-IPCs carrying miR-375 and anti-miR-7 after five weeks of transplantation when compared to the untreated diabetic mice. For the first time, this study showed that the two-component microfluidic system is useful for supporting the Collagen-Alginate fiber-entrapped IPCs and the miRNAs-based cell therapy.ConclusionsOverall data show that the IPCs encapsulation by the microfluidic system can support the cells in terms of morphology and biological function and their efficiency for controlling the hyperglycemia condition in diabetic mice.

Mentha pulegium Aqueous Extract Exhibits Antidiabetic and Hepatoprotective Effects in Streptozotocin-Induced Diabetic Rats

2019 ◽  
Vol 19 (3) ◽  
pp. 292-301
Author(s):  
Omar Farid ◽  
Naoufel Ali Zeggwagh ◽  
Fadwa EL Ouadi ◽  
Mohamed Eddouks
Keyword(s):  
Blood Glucose ◽  
Glucose Tolerance ◽  
Aqueous Extract ◽  
Morphometric Analysis ◽  
Diabetic Rats ◽  
Mentha Pulegium ◽  
Glucose Levels ◽  
Histological Sections ◽  
Blood Glucose Levels ◽  
Liver And Pancreas

Objective: The aim of this work was to evaluate the antihyperglycemic activity of aerial parts aqueous extract (A.P.A.E) of Mentha pulegium (M. pulegium) on blood glucose levels in normal and streptozotocin(STZ)-induced diabetic rat. The glucose tolerance was evaluated in normal rats. Moreover, the histological sections and morphometric analysis at the liver and pancreas have been carried out in this investigation both in normal and STZ-diabetic rats. Methods: The effect of A.P.A.E of M. pulegium (20 mg/kg) on blood glucose levels was investigated in normal and diabetic rats (n=6). Histopathological changes in liver and pancreas were examined under phase contrast microscope and a preliminary screening for various bioactive constituents was realized according to standard methods. Key Findings: Both single and repeated oral administration of A.P.A.E (20 mg/kg) caused a significant reduction in blood glucose levels in STZ-diabetic rats (p<0.0001). The morphometric analysis and histological sections realized in pancreas and liver have showed the beneficial effect of the A.P.A.E in cellular population. According to oral glucose tolerance test (OGTT), the aqueous extract has revealed an improvement of glucose tolerance in normal rat. Furthermore, the preliminary phytochemical screening of A.P.A.E of M. pulegium has demonstrated the presence of various metabolite compounds including polyphenols, flavonoids, terpenoids tannins, cyanidins, sesquiterpenes, and glycosides. Conclusion: We conclude that the A.P.A.E of M. pulegium (20 mg/kg) exhibits a potent antihyperglycemic activity in STZ diabetic rats.

scholarly journals PENGARUH JUS LIDAH BUAYA TERHADAP KADAR GLUKOSA DARAH PUASA DAN 2 JAM PP(Post Prandial) PADA PENDERITA DIABETES MELITUS

2019 ◽  
Vol 10 (1) ◽  
pp. 77
Author(s):  
Febria Syafyu Sari ◽  
Ridhyalla Afnuhazi
Keyword(s):  
Diabetes Mellitus ◽  
Blood Glucose ◽  
Blood Sugar ◽  
Fasting Blood Glucose ◽  
Aloe Vera ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Patients With Diabetes ◽  
Paired T Test ◽  
Diabetes Melitus

ABSTRAK Diabetes Melitus merupakan penyakit yang paling menonjol yang disebabkan oleh gagalnya pengaturan gula darah. Lidah buaya berkhasiat untuk menurunkan kadar gula dalam darah bagi penderita diabetes dan dapat mengontrol tekanan darah. Tujuan penelitian untuk mengetahui pengaruh jus lidah buaya (AloeBarbadensis Miller) terhadap penurunan glukosa darah puasa GDP) dan 2 Jam PP (Post Prendial) pada penderita DM (Diabetes Melitus). Desain penelitian merupakan Quasi Eksperimental dengan pendekatan one group pretest – postest design. Sampel terbagi menjadi 14 responden. Data dianalisis dengan paired t-test. Hasil menunjukan rata-rata penurunan glukosa darah puasa pada intervensi (28,42 gr/dl) dan glukosa darah 2 jam pp pada intervensi (40,57 gr/dl). Untuk analisis bivariat terdapat perbedaan antara glukosa puasa dan 2 jam pp dengan glukosa darah puasa GDP) dan 2 Jam PP (Post Prendial) pada penderita DM (Diabetes Melitus). Kesimpulan didapatkanlidah buaya dapat menurunkan kadar glukosa darah.Berdasarkan hasil penelitian jus lidah buaya dapat menjadi salah satu alternatif keperawatan non farmakologi dalam penyakit diabetes melitus. Kata Kunci : Lidah Buaya ; Diabetes Mellitus THE EFFECT OF VEGETABLE VOCATIONAL JUICE ON FAST BLOOD GLUCOSE LEVELS AND 2 HOURS OF PP (Post Prandial) IN DIABETES MELLITUS  ABSTRACT Diabetes mellitus is the most prominent disease caused by the failure of blood sugar regulation. Aloe vera is efficacious can to  reduce blood sugar levels for diabetics and can control blood pressure. The purpose of this study was to determine the effect of Aloe Barbadensis Miller on the reduction of fasting blood glucose GDP and 2 hours of PP (post prendial) in patients with diabetes mellitus. The research design is Experimental Quasi with one group pretest - postest design approach. The sample is divided into 14 respondents. Data were analyzed by paired t-test. The results showed an average decrease in fasting blood glucose at intervention (28.42 gr / dl) and 2 hours pp blood glucose at intervention (40.57 gr / dl). For bivariate analysis there was a difference between fasting glucose and 2 hours pp with fasting blood glucose GDP) and 2 hours PP (Post Prendial) in patients with diabetes mellitus. The conclusion is that aloe vera can reduce blood glucose levels. Based on the results of research on aloe vera juice can be an alternative non-pharmacological nursing in diabetes mellitus. Keywords: Aloe Vera ; Diabetes Mellitus

scholarly journals EFFECT OF THE AQUEOUS EXTRACT OF CLERODENDRUM THOMSONIAE LINN (VERBENACEAE) LEAVES ON TYPE 2 DIABETIC WISTAR RATS INDUCED BY THE MACAPOS1 TYPE DIET AND DEXAMETHASONE

2021 ◽  
Author(s):  
Eric Martial Deutchoua Ngounou ◽  
Yannick Dimitry Mang ◽  
Faustin Dongmo ◽  
Oumar Waassili Ibrahim Malla ◽  
Sélestin Sokeng Dongmo ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Peer Review ◽  
Aqueous Extract ◽  
Reduction Rate ◽  
Diabetic Rats ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Significant Difference ◽  
Type 2 Diabetic

Aim and objective: Clerodendrum thomsoniae leaves are used in Cameroon to manage diabetes and its related disorders. The study aimed at investigating the antidiabetic effect of the aqueous extract on diet and dexamethasone induced diabetic rats. Methods: Young mature leaves of C thomsoniae were dried, finely powdered and submitted to aqueous extraction. The dehydrated extract was tested in rats at 3 doses 312.5, 625 and 1250 mg/kg based on the local use of the plant. The effect of the extract on the fasting blood glucose in normoglycemic rats and MACAPOS 1 type diet induced diabetic rats, using respectively glibenclamide and metformin as positive control groups, were investigated. Results: AECT significantly reduced blood glucose levels in normoglycemic rats (p<0.05) two hours after administration, from 83±2 mg/dL to 57.39±1.7 mg/dL with the dose of 1250 mg/kg. given the highest reduction rate of 30.86%. In normoglycemic rats 30 minutes after oral glucose overload, the maximum reduction rate was observed with glibenclamide 5 mg / kg and calculated at 49.90% followed by 36.39%, for the extract at 1250 mg / kg. After 30 days of repeated oral administration, AECT produced a reduction on blood glucose levels (p<0.05) in type 2 diabetic rats. This reduction in blood sugar was much more expressed with the dose of 1250mg/kg (73.52±0.71 mg/dL) followed by metformin 38mg/kg (70.21±0.89 mg/dL) as the normal control with no significant difference (P < 0.05). Conclusion: These results show that the antidiabetic activity of AECT can be explained by insulin stimulating effect, also give support to the traditional use of this plant.                   Peer Review History: Received 11 May 2021; Revised 17 June; Accepted 27 June, Available online 15 July 2021 Academic Editor: Dr. Asia Selman Abdullah,  Al-Razi university, Department of Pharmacy, Yemen, [email protected] UJPR follows the most transparent and toughest ‘Advanced OPEN peer review’ system. The identity of the authors and, reviewers will be known to each other. This transparent process will help to eradicate any possible malicious/purposeful interference by any person (publishing staff, reviewer, editor, author, etc) during peer review. As a result of this unique system, all reviewers will get their due recognition and respect, once their names are published in the papers. We expect that, by publishing peer review reports with published papers, will be helpful to many authors for drafting their article according to the specifications. Auhors will remove any error of their article and they will improve their article(s) according to the previous reports displayed with published article(s). The main purpose of it is ‘to improve the quality of a candidate manuscript’. Our reviewers check the ‘strength and weakness of a manuscript honestly’. There will increase in the perfection, and transparency.  Received file:                Reviewer's Comments: Average Peer review marks at initial stage: 6.5/10 Average Peer review marks at publication stage: 8.0/10 Reviewer(s) detail: Dr. Terhemen Festus Swem, Department of Veterinary Physiology and Biochemistry, College of Veterinary Medicine, Federal University of Agriculture, Makurdi, Benue State, Nigeria, [email protected] Taha A.I. El Bassossy, Medicinal and Aromatic Plants Department, Desert Research Center, Cairo, Egypt, [email protected] Prof. Dr. Ali Gamal Ahmed Al-kaf, Sana'a university, Yemen, [email protected]   Similar Articles: ANTIDIABETIC AND ANTIHYPERLIPIDEMIC ACTIVITY OF DRACAENA CINNABARI BALF. RESIN ETHANOLIC EXTRACT OF SOQATRA ISLAND IN EXPERIMENTAL ANIMALS THE SCOPING REVIEW OF CHINESE AND WESTERN MEDICINE TREATMENT OF DIABETIC FOOT IN ASIA ANTIHYPERGLYCEMIC AND ANTI-OXIDANT POTENTIAL OF ETHANOL EXTRACT OF VITEX THYRSIFLORA LEAVES ON DIABETIC RATS EFFECTS OF EMODIN ON BLOOD GLUCOSE AND BODY WEIGHT IN TYPE 1 DIABETIC RATS

scholarly journals Physiological study to investigate the activity of an aqueous extract ofCinnamomum cassiabark on the blood glucose levels in healthy and diabetic rats induced by streptozotocin (stz)

2016 ◽  
Vol 13 (4) ◽  
pp. 681-693
Author(s):  
Baghdad Science Journal
Keyword(s):  
Blood Glucose ◽  
Oral Administration ◽  
Aqueous Extract ◽  
Diabetic Rats ◽  
Physiological Study ◽  
Cinnamomum Cassia ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Single Oral Administration

The present study was investigated the activity of aqueous extract from Cinnamomum cassia bark on the blood glucose levels in healthy and diabetic rats induced by Streptozotcin (STZ). In healthy rats the blood glucose levels were slightly decreased after six hoursof single oral administration with dose (25 mg/kg) of body wight, as well as four weeks after twice daily repeated oral administration of aqueous extract of Cinnamomum cassia bark. In streptozotocin induced diabetic rats we absorved high significant decreased (p

TYPE 2 DIABETES MELLITUS;

2018 ◽  
Vol 21 (05) ◽  
pp. 1005-1014
Author(s):  
Jamil A. Malik ◽  
Tuba Mumtaz ◽  
Samsaam Ali Haider
Keyword(s):  
Blood Glucose ◽  
Emotional Problem ◽  
Emotional Problems ◽  
Treatment Modalities ◽  
Routine Practice ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Obese Class ◽  
Patients With Diabetes ◽  
Effective Instrument

Objectives: The study is aimed to assess emotional problem of patients with Type2diabetes by translating and validating PAID. Associations between PAID and blood glucoselevels, and differences in prevalence of emotional problems across treatment modalities andBMI. Data Sources: Data was collected from various outpatients of Rawalpindi and Islamabad.Sample and Procedure: The sample of study comprised of 300 patients with diabetes bothmale = 135 (45%) and female n=165 (55%). Formal approval was taken from authorities atrespective hospitals and after taking informed consent patients were interviewed. Period:Data collection was completed during Feb to April, 2013. Material and Method: Patients wereasked to fill in demographic sheet along with Translated PAID scale. Latest blood glucose levelswere collected from their medical records whereas BMI was calculated by measuring patients’weight and height. Results: Results showed that emotional problems as assessed by PAID arepositively correlated with blood glucose levels (r=.12 to r=.19, p<.05). Additionally, patientswith IV modality of treatment (i.e., taking insulin shots) suffered significantly high (MD=4.88,p=.01) from emotional problems and patients with normal BMI and Obese Class-II have are athigher risk of emotional problem. Finally, patients rated serious concerns on diabetes-relatedissues like lifelong maintenance, future complications, depression and anxiety, and satisfactionwith physician. Conclusions: PAID is a very effective instrument in identifying emotionalproblems of diabetes patients related to adherence and compliance to their treatment regimeand hence it shall be used by physicians and health professional in routine practice.

Effect of diet on fasting blood glucose levels in dairy calves 1 to 13 weeks old

1963 ◽  
Vol 18 (6) ◽  
pp. 1221-1225 ◽  
Author(s):  
John T. Attebery ◽  
Harry W. Colvin
Keyword(s):  
Blood Glucose ◽  
Fasting Blood Glucose ◽  
Experimental Period ◽  
Constant Level ◽  
Dairy Calves ◽  
Stomach Tube ◽  
Glucose Levels ◽  
Fasting Level ◽  
Blood Glucose Levels ◽  
Milk Solids

Fourteen male Holstein-Fresian calves, 3–4 days of age, were placed on one of four diets for a 13-week experimental period. The diets included the following: milk and milk solids; milk and grain; milk, grain, and hay, inoculated (rumen ingesta from a mature steer introduced via stomach tube into the rumen of an experimental calf); or milk and grain, inoculated. Significant differences were noted in the effect of diet, age, and the interaction of diet and age on the fasting level of blood glucose. The calves on the diet containing milk and milk solids maintained a constant level of fasting blood glucose; however, the inclusion of grain and hay in the diet resulted in a decline of the fasting blood glucose. The rumen inoculation of one of the groups of calves on the milk and grain diet did not result in a fasting blood glucose which was statistically different from that of the calves not inoculated. ruminant blood glucose; diet and age interaction; milk solids Submitted on February 26, 1963

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