scholarly journals Co-registered photoacoustic and ultrasound imaging for tongue cancer detection

2018 ◽  
Vol 11 (03) ◽  
pp. 1850008 ◽  
Author(s):  
Heng Guo ◽  
Weizhi Qi ◽  
Ming He ◽  
Jian Rong ◽  
Lei Xi
Keyword(s):  
Ultrasound Imaging ◽  
Structural Information ◽  
Tongue Cancer ◽  
Early Stage ◽  
Signal To Noise Ratio ◽  
Imaging Techniques ◽  
Common Disease ◽  
High Morbidity ◽  
Human Tongue

Tongue cancer is an increasingly common disease with high morbidity. Besides clinical observation, biomedical imaging techniques have been investigated for early detection of tongue cancer. In this paper, we proposed a co-registered dual-modality photoacoustic (PA) and ultrasound imaging technique to simultaneously map the functional and structural information of human tongue, which has the potential to detect and diagnose tongue cancer in early stage. The imaging probe comprises a 20-MHz side-view focused transducer for ultrasound imaging and PA detection, a light path constructed by a multimode optical fiber, and a prism for PA illumination. Phantom experiments were conducted to evaluate the performance of the system including penetration depth, spatial resolution and signal-to-noise ratio. In vivo imaging of animal tumor and human tongue was carried out to show the feasibility of the proposed technique to detect tumor lesions in human tongue. The results of phantom and in vivo experiments suggest that the proposed technique has the potential to detect the early-stage cancer lesions in human tongue.

scholarly journals Tumorigenicity and Validity of Fluorescence Labelled Mesenchymal and Epithelial Human Oral Cancer Cell Lines in Nude Mice

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Wei Xin Cai ◽  
Li Wu Zheng ◽  
Li Ma ◽  
Hong Zhang Huang ◽  
Ru Qing Yu ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Cell Lines ◽  
Cancer Cell ◽  
Nude Mice ◽  
Tongue Cancer ◽  
Cancer Cell Lines ◽  
Fluorescent Imaging ◽  
Cell Phenotype ◽  
Human Tongue

Tumorigenicity and metastatic activity can be visually monitored in cancer cells that were labelled with stable fluorescence. The aim was to establish and validate local and distant spread of subcutaneously previously injected fluorescence transduced human tongue cancer cell lines of epithelial and mesenchymal phenotype in nude mice. A total of 32 four-week-old male athymic Balb/c nude mice were randomly allocated into 4 groups (n=8). A single dose of 0.3 mL PBS containing 1 × 107 of four different cancer cell-lines (UM1, UM1-GFP, UM2, and UM2-RFP) was injected subcutaneously into the right side of their posterolateral back. Validity assessment of the labelled cancer cells’ tumorigenicity was assessed by physical examination, imaging, and histology four weeks after the injection. The tumor take rate of cancer cells was similar in animals injected with either parental or transduced cancer cells. Transduced cancer cells in mice were easily detectable in vivo and after cryosection using fluorescent imaging. UM1 cells showed increased tumor take rate and mean tumor volume, presenting with disorganized histopathological patterns. Fluorescence labelled epithelial and mesenchymal human tongue cancer cell lines do not change in tumorigenicity or cell phenotype after injection in vivo.

scholarly journals Rationally Designing Simple Rod-Like Amphiphilic NIR-Emissive AIE Probes for Precise In-Vivo Detection of Aβ Fibrils/Plaques at A Super-Early Stage

2021 ◽  
Author(s):  
Yipu Wang ◽  
Dong Mei ◽  
Xinyi Zhang ◽  
Da-Hui Qu ◽  
Ju Mei ◽  
...  
Keyword(s):  
High Performance ◽  
Ex Vivo ◽  
Early Stage ◽  
Signal To Noise Ratio ◽  
High Signal ◽  
In Vivo Detection ◽  
Different Strains ◽  
Aβ Fibrils

With increase of social aging, Alzheimer's disease (AD) has been one of the serious diseases threatening human health. The occurrence of A<i>β </i>fibrils<i> </i>or plaques is recognized as the hallmark of AD.<i> </i>Currently, optical imaging has stood out to be a promising technique for the imaging of A<i>β</i> fibrils/plaques and the diagnosis of AD. However, restricted by their poor blood-brain barrier (BBB) penetrability, short-wavelength excitation and emission, and aggregation-caused quenching (ACQ) effect, the clinically used gold-standard optical probes such as <a>thioflavin</a> T (ThT) and thioflavin S (ThS), are not effective enough in the early diagnosis of AD <i>in vivo</i>. Herein, we put forward an “all-in-one” design principle and demonstrate its feasibility in developing high-performance fluorescent probes which are specific to A<i>β</i> fibrils/plaques and promising for super-early <i>in</i>-<i>vivo</i> diagnosis of AD. As a proof of concept, a simple rod-like amphiphilic NIR fluorescent AIEgen, i.e., AIE-CNPy-AD, is developed by taking the specificity, BBB penetration ability, deep-tissue penetration capacity, high signal-to-noise ratio (SNR) into consideration. AIE-CNPy-AD is constituted by connecting the electron-donating and accepting moieties through single bonds and tagging with a propanesulfonate tail, giving rise to the NIR fluorescence, aggregation-induced emission (AIE) effect, amphiphilicity, and rod-like structure, which in turn result in high binding-affinity and excellent specificity to A<i>β</i> fibrils/plaques, satisfactory ability to penetrate BBB and deep tissues, ultrahigh SNR and sensitivity, and high-fidelity imaging capability. <i>In-vitro, ex-vivo,</i> and <i>in-vivo</i> <a>identifying of A<i>β</i> fibrils/plaques</a> in different strains of mice indicate that AIE-CNPy-AD holds the universality to the detection of A<i>β</i> fibrils/plaques. It is noteworthy that AIE-CNPy-AD is even able to trace the small and sparsely distributed A<i>β</i> fibrils/plaques in very young AD model mice such as 4-month-old APP/PS1 mice which are reported to be the youngest mice to have A<i>β</i> deposits in brains, suggesting its great potential in diagnosis and intervention of AD at a super-early stage.

Cordycepin induces apoptosis in human tongue cancer cells in vitro and has antitumor effects in vivo

2020 ◽  
Vol 118 ◽  
pp. 104846
Author(s):  
Qingwei Zheng ◽  
Jing Sun ◽  
Wenli Li ◽  
Shuangnan Li ◽  
Kai Zhang
Keyword(s):  
Cancer Cells ◽  
Tongue Cancer ◽  
Antitumor Effects ◽  
Human Tongue

scholarly journals Cucurbitacin B Inhibits Cell Proliferation by Regulating X-Inactive Specific Transcript Expression in Tongue Cancer

2021 ◽  
Vol 11 ◽  
Author(s):  
Boqiang Tao ◽  
Dongxu Wang ◽  
Shuo Yang ◽  
Yingkun Liu ◽  
Han Wu ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Tongue Cancer ◽  
P53 Protein ◽  
Tumor Development ◽  
Migration And Invasion ◽  
Cucurbitacin B ◽  
Specific Transcript ◽  
Human Tongue

Cucurbitacin B (CuB), a natural product, has anti-tumor effects on various cancers. In order to investigate the expression of long non-coding RNAs (lncRNA), we carried out RNA sequencing (RNA-seq) and quantitative PCR (qPCR). The data indicated that CAL27 and SCC9 tongue squamous cell carcinoma (TSCC) cells had reduced expression of X-inactive specific transcript (XIST) after CuB treatment. Moreover, our results showed increased expression of XIST in human tongue cancer. In this study, CuB treatment inhibited proliferation, migration and invasion of SCC9 cells, and induced cellular apoptosis. Interestingly, knockdown of XIST led to inhibition of cell proliferation and induced apoptosis in vitro. In addition, reduced expression of XIST suppressed cell migration and invasion. MicroRNA 29b (miR-29b) was identified as a direct target of XIST. Previous reports indicated that miR-29b regulates p53 protein. Our results suggest that increased expression of miR-29b induces cell apoptosis through p53 protein. The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (CRISPR/Cas9) system validated the role of XIST knockout in tumor development in vivo. Together, these results suggest that CuB exerts significant anti-cancer activity by regulating expression of XIST via miR-29b.

scholarly journals Evaluation of 2D super-resolution ultrasound imaging of the rat renal vasculature using ex vivo micro-computed tomography

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Sofie Bech Andersen ◽  
Iman Taghavi ◽  
Hans Martin Kjer ◽  
Stinne Byrholdt Søgaard ◽  
Carsten Gundlach ◽  
...  
Keyword(s):  
Ultrasound Imaging ◽  
Ex Vivo ◽  
Imaging Techniques ◽  
Rat Kidney ◽  
Vascular Anatomy ◽  
Super Resolution ◽  
Sprague Dawley ◽  
Micro Computed Tomography ◽  
Renal Vasculature

AbstractSuper-resolution ultrasound imaging (SRUS) enables in vivo microvascular imaging of deeper-lying tissues and organs, such as the kidneys or liver. The technique allows new insights into microvascular anatomy and physiology and the development of disease-related microvascular abnormalities. However, the microvascular anatomy is intricate and challenging to depict with the currently available imaging techniques, and validation of the microvascular structures of deeper-lying organs obtained with SRUS remains difficult. Our study aimed to directly compare the vascular anatomy in two in vivo 2D SRUS images of a Sprague–Dawley rat kidney with ex vivo μCT of the same kidney. Co-registering the SRUS images to the μCT volume revealed visually very similar vascular features of vessels ranging from ~ 100 to 1300 μm in diameter and illustrated a high level of vessel branching complexity captured in the 2D SRUS images. Additionally, it was shown that it is difficult to use μCT data of a whole rat kidney specimen to validate the super-resolution capability of our ultrasound scans, i.e., validating the actual microvasculature of the rat kidney. Lastly, by comparing the two imaging modalities, fundamental challenges for 2D SRUS were demonstrated, including the complexity of projecting a 3D vessel network into 2D. These challenges should be considered when interpreting clinical or preclinical SRUS data in future studies.

scholarly journals Rationally Designing Simple Rod-Like Amphiphilic NIR-Emissive AIE Probes for Precise In-Vivo Detection of Aβ Fibrils/Plaques at A Super-Early Stage

2021 ◽  
Author(s):  
Yipu Wang ◽  
Dong Mei ◽  
Xinyi Zhang ◽  
Da-Hui Qu ◽  
Ju Mei ◽  
...  
Keyword(s):  
High Performance ◽  
Ex Vivo ◽  
Early Stage ◽  
Signal To Noise Ratio ◽  
High Signal ◽  
In Vivo Detection ◽  
Different Strains ◽  
Aβ Fibrils

With increase of social aging, Alzheimer's disease (AD) has been one of the serious diseases threatening human health. The occurrence of A<i>β </i>fibrils<i> </i>or plaques is recognized as the hallmark of AD.<i> </i>Currently, optical imaging has stood out to be a promising technique for the imaging of A<i>β</i> fibrils/plaques and the diagnosis of AD. However, restricted by their poor blood-brain barrier (BBB) penetrability, short-wavelength excitation and emission, and aggregation-caused quenching (ACQ) effect, the clinically used gold-standard optical probes such as <a>thioflavin</a> T (ThT) and thioflavin S (ThS), are not effective enough in the early diagnosis of AD <i>in vivo</i>. Herein, we put forward an “all-in-one” design principle and demonstrate its feasibility in developing high-performance fluorescent probes which are specific to A<i>β</i> fibrils/plaques and promising for super-early <i>in</i>-<i>vivo</i> diagnosis of AD. As a proof of concept, a simple rod-like amphiphilic NIR fluorescent AIEgen, i.e., AIE-CNPy-AD, is developed by taking the specificity, BBB penetration ability, deep-tissue penetration capacity, high signal-to-noise ratio (SNR) into consideration. AIE-CNPy-AD is constituted by connecting the electron-donating and accepting moieties through single bonds and tagging with a propanesulfonate tail, giving rise to the NIR fluorescence, aggregation-induced emission (AIE) effect, amphiphilicity, and rod-like structure, which in turn result in high binding-affinity and excellent specificity to A<i>β</i> fibrils/plaques, satisfactory ability to penetrate BBB and deep tissues, ultrahigh SNR and sensitivity, and high-fidelity imaging capability. <i>In-vitro, ex-vivo,</i> and <i>in-vivo</i> <a>identifying of A<i>β</i> fibrils/plaques</a> in different strains of mice indicate that AIE-CNPy-AD holds the universality to the detection of A<i>β</i> fibrils/plaques. It is noteworthy that AIE-CNPy-AD is even able to trace the small and sparsely distributed A<i>β</i> fibrils/plaques in very young AD model mice such as 4-month-old APP/PS1 mice which are reported to be the youngest mice to have A<i>β</i> deposits in brains, suggesting its great potential in diagnosis and intervention of AD at a super-early stage.

scholarly journals Enhanced X-ray Attenuating Efficiency of Silicon Dioxide Nanoparticles with Cesium Lead Bromide and 2,5-Diphenyloxazole Co-Embedded Therein

Crystals ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 1531
Author(s):  
Geunpyo Choe ◽  
Hyemin Kwon ◽  
Ilhwan Ryu ◽  
Sanggyu Yim
Keyword(s):  
Silicon Dioxide ◽  
Early Stage ◽  
Imaging Techniques ◽  
Fluorescence Emission ◽  
Hounsfield Unit ◽  
Sio2 Nanoparticles ◽  
Silicon Dioxide Nanoparticles ◽  
X Ray ◽  
Lead Bromide

An X-ray-attenuation-based in vivo imaging can be a promising candidate for real-time detection of cancer in an early stage due to its significantly longer penetration depth compared to currently investigated fluorescence-emission-based imaging techniques. It has recently been demonstrated that this novel concept of imaging is feasible using cesium lead bromide (CPB) quantum dots (QDs) stably embedded in silicon dioxide (SiO2) nanoparticles (NPs). However, further improvements are necessary to realize its practical use, especially in terms of X-ray attenuation efficiency. In this study, we have found that the X-ray attenuation capability of CPB/SiO2 NPs was significantly enhanced by embedding an organic X-ray scintillator, 2,5-diphenyloxazole (PPO), together with CPB QDs in the NPs. The embedment not only solved the water dispersibility and stability problem of PPO, but also significantly increased the Hounsfield unit of the NPs, which was proportional to the degree of X-ray attenuation, by 2.7 times.

scholarly journals Distinct role of heme oxygenase-1 in early- and late-stage intracerebral hemorrhage in 12-month-old mice

2016 ◽  
Vol 37 (1) ◽  
pp. 25-38 ◽  
Author(s):  
Zhen Zhang ◽  
Yuejia Song ◽  
Ze Zhang ◽  
Danyang Li ◽  
Hong Zhu ◽  
...  
Keyword(s):  
Intracerebral Hemorrhage ◽  
Heme Oxygenase ◽  
Early Stage ◽  
Heme Oxygenase 1 ◽  
High Morbidity ◽  
Key Enzyme ◽  
Neurologic Function

Intracerebral hemorrhage (ICH) is a devastating form of stroke with high morbidity and mortality. Heme oxygenase-1 (HO-1), the key enzyme in heme degradation, is highly expressed after ICH, but its role is still unclear. In this study, we used an HO-1 inducer and inhibitor to test the role of HO-1 in different stages of ICH in vivo and in vitro. In the early stage of ICH, high HO-1 expression worsened the outcomes of mice subjected to the collagenase-induced ICH model. HO-1 increased brain edema, white matter damage, neuronal death, and neurobehavioral deficits. Furthermore, elevated HO-1 increased inflammation, oxidative stress, matrix metalloproteinase-9/2 activity, and iron deposition. In the later stage of ICH, long-term induction of HO-1 increased hematoma absorption, angiogenesis, and recovery of neurologic function. We conclude that HO-1 activation mediates early brain damage after ICH but promotes neurologic function recovery in the later stage of ICH.

scholarly journals Recent Advances in Molecular Magnetic Resonance Imaging of Liver Fibrosis

2015 ◽  
Vol 2015 ◽  
pp. 1-12 ◽  
Author(s):  
Zhiming Li ◽  
Jihong Sun ◽  
Xiaoming Yang
Keyword(s):  
Liver Fibrosis ◽  
Mr Imaging ◽  
Early Stage ◽  
Imaging Techniques ◽  
High Morbidity ◽  
Novel Technologies ◽  
Recent Advances ◽  
Long Term Follow Up ◽  
Life Threatening ◽  
Liver Biopsies

Liver fibrosis is a life-threatening disease with high morbidity and mortality owing to its diverse causes. Liver biopsy, as the current gold standard for diagnosing and staging liver fibrosis, has a number of limitations, including sample variability, relatively high cost, an invasive nature, and the potential of complications. Most importantly, in clinical practice, patients often reject additional liver biopsies after initiating treatment despite their being necessary for long-term follow-up. To resolve these problems, a number of different noninvasive imaging-based methods have been developed for accurate diagnosis of liver fibrosis. However, these techniques only reflect morphological or perfusion-related alterations in the liver, and thus they are generally only useful for the diagnosis of late-stage liver fibrosis (liver cirrhosis), which is already characterized by “irreversible” anatomic and hemodynamic changes. Thus, it is essential that new approaches are developed for accurately diagnosing early-stage liver fibrosis as at this stage the disease may be “reversed” by active treatment. The development of molecular MR imaging technology has potential in this regard, as it facilitates noninvasive, target-specific imaging of liver fibrosis. We provide an overview of recent advances in molecular MR imaging for the diagnosis and staging of liver fibrosis and we compare novel technologies with conventional MR imaging techniques.

27. Low Frequency Ultrasound Imaging of Apoptosis in Tumor Response: Non-Invasive Monitoring of Chemotherapy Effects

2018 ◽  
Author(s):  
Stephanie Zhou
Keyword(s):  
Cell Death ◽  
Ultrasound Imaging ◽  
Structural Changes ◽  
Imaging Techniques ◽  
Low Frequency ◽  
Acoustic Properties ◽  
High Frequency Ultrasound ◽  
Noninvasive Methods ◽  
Frequency Ultrasound

Due to the growing costs of chemotherapy, previous imaging techniques such as MRI or CT scans have become too time-consuming in the assessment of chemotherapy’s effects. With results generated about 2 weeks later, the patient is exposed to the negative side effects of these medications with the possibility that chemotherapy may not be improving their prognosis. Thus, ultrasound has become increasingly popular as a method to determine chemotherapy’s effect on tumors within 24 hours. Both low and high-frequency ultrasound are novel, noninvasive methods for detecting cell death based on changes in cell morphology. Condensation, fragmentation and alterations in the cell nucleus during apoptosis are linked to changes in the cell’s acoustic properties, as indicated by experimental evidence. In this study, quantitative ultrasound was used to follow responses of tumor models to chemotherapy in vivo. As studies have shown that structural changes can occur as early as 24 hours after treatment, ultrasound imaging was administered before and 24 hours after treatment. Changes in ultrasound parameters such as spectral slope, Y-intercept, and midband fit were analyzed relative to pretreatment control data and when compared to changes in the tumors seen through cell staining, changes consistent with cell death were observed.

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