Body composition, food intake, and brown fat thermogenesis in pregnant Djungarian hamsters

1987 ◽  
Vol 253 (2) ◽  
pp. R314-R320 ◽  
Author(s):  
J. E. Schneider ◽  
G. N. Wade
Keyword(s):  
Body Weight ◽  
Food Intake ◽  
Late Pregnancy ◽  
Brown Fat ◽  
Djungarian Hamster ◽  
Body Lipid ◽  
Mitochondrial Content ◽  
Pregnant Females ◽  
Balancing Energy ◽  
Lipid Stores

Strategies for balancing energy storage and expenditure during pregnancy were examined in the Djungarian hamster. In expt 1, pregnant hamsters lost nearly 50% of their body lipid stores, even though they showed significant increases in body weight and food intake during the latter half of pregnancy. The increase in body weight was accounted for by the growth of the uteri, fetuses, and placentas. The water and fat-free dry contents of the maternal carcasses did not differ from those of the unmated controls. In expt 2, brown fat cytochrome-c oxidase activity (mitochondrial content) was significantly lower in the late- but not early-pregnant females relative to the nonpregnant controls. Specific GDP-binding levels did not differ significantly among these groups. Thus an overall decrease in total thermogenic activity in brown fat would be expected during late pregnancy. The loss of carcass lipid, despite decreased brown fat thermogenesis and increased food intake, suggests that substantial increases in energy expenditure occur in pregnant females that are not related to heat production in brown fat. The present results are not consistent with traditional models of energy balance during pregnancy. Some of the inconsistencies may be related to differences between hoarding and nonhoarding families of rodents, whereas others may be due to the fact that the traditional model is based on a possibly exceptional species, the laboratory rat.

scholarly journals Dipeptidyl peptidase IV inhibitor lowers PPARγ agonist-induced body weight gain by affecting food intake, fat mass, and beige/brown fat but not fluid retention

2014 ◽  
Vol 306 (4) ◽  
pp. E388-E398 ◽  
Author(s):  
Takahiro Masuda ◽  
Yiling Fu ◽  
Akiko Eguchi ◽  
Jan Czogalla ◽  
Michael A. Rose ◽  
...  
Keyword(s):  
Body Weight ◽  
Adipose Tissue ◽  
Food Intake ◽  
Fat Mass ◽  
Fluid Retention ◽  
Dipeptidyl Peptidase Iv ◽  
Brown Fat ◽  
Antidiabetic Drugs ◽  
Dpp Iv ◽  
Pparγ Agonist

Peroxisome proliferator-activated receptor-γ (PPARγ) agonists like pioglitazone (PGZ) are effective antidiabetic drugs, but they induce fluid retention and body weight (BW) gain. Dipeptidyl peptidase IV (DPP IV) inhibitors are antidiabetic drugs that enhance renal Na+ and fluid excretion. Therefore, we examined whether the DPP IV inhibitor alogliptin (ALG) ameliorates PGZ-induced BW gain. Male Sv129 mice were treated with vehicle (repelleted diet), PGZ (220 mg/kg diet), ALG (300 mg/kg diet), or a combination of PGZ and ALG (PGZ + ALG) for 14 days. PGZ + ALG prevented the increase in BW observed with PGZ but did not attenuate the increase in body fluid content determined by bioimpedance spectroscopy (BIS). BIS revealed that ALG alone had no effect on fat mass (FM) but enhanced the FM-lowering effect of PGZ; MRI analysis confirmed the latter and showed reductions in visceral and inguinal subcutaneous (sc) white adipose tissue (WAT). ALG but not PGZ decreased food intake and plasma free fatty acid concentrations. Conversely, PGZ but not ALG increased mRNA expression of thermogenesis mediator uncoupling protein 1 in epididymal WAT. Adding ALG to PGZ treatment increased the abundance of multilocular cell islets in sc WAT, and PGZ + ALG increased the expression of brown-fat-like “beige” cell marker TMEM26 in sc WAT and interscapular brown adipose tissue and increased rectal temperature vs. vehicle. In summary, DPP IV inhibition did not attenuate PPARγ agonist-induced fluid retention but prevented BW gain by reducing FM. This involved ALG inhibition of food intake and was associated with food intake-independent synergistic effects of PPARγ agonism and DPP-IV inhibition on beige/brown fat cells and thermogenesis.

scholarly journals SAT-603 Growth Hormone-Releasing Hormone (GHRH) Antagonists Stimulate Feeding in Mice

2020 ◽  
Vol 4 (Supplement_1) ◽  
Author(s):  
Sheila Leone ◽  
Lucia Recinella, PharmD ◽  
Annalisa Chiavaroli, PharmD ◽  
Giustino Orlando, PharmD ◽  
Claudio Ferrante, PharmD ◽  
...  
Keyword(s):  
Gene Expression ◽  
Growth Hormone ◽  
Body Weight ◽  
Locomotor Activity ◽  
Food Intake ◽  
Brown Fat ◽  
Growth Hormone Releasing Hormone ◽  
Antitumor Effects ◽  
Releasing Hormone ◽  
Fat Depots

Abstract Growth hormone-releasing hormone (GHRH) is a hypothalamic neuropeptide which stimulates the synthesis and secretion of growth hormone (GH) in pituitary gland. GHRH was also found to modulate food intake in mammals. MIA-690 is a synthetic GHRH antagonist of the Miami (MIA) series with potent antitumor effects. To date, its role in hypothalamic feeding modulation has not been evaluated. In the present study, we aimed to investigate the effects of chronic MIA-690 administration on feeding behavior, locomotor activity and hypothalamic dopamine (DA), norepinephrine (NE), serotonin (5-hydroxytriptamine, 5-HT), orexigenic peptides [agouti-related peptide (AgRP) and neuropeptide Y (NPY)] and anorexigenic peptides [cocaine and amphetamine-regulated transcript (CART) and proopiomelanocortin (POMC)] activity. Adult C57/BL6 mice were treated daily for 4 weeks by subcutaneous administration of (5 µg) MIA-690 or vehicle solution. Food intake and body weight were recorded every 4 days throughout the study. Immediately after the last injection, locomotor activity in the home cage was recorded, and thereafter animals were sacrificed. Visceral, subcutaneous and brown fat depots were quickly excised and weighed. Hypothalamus was also dissected for evaluating gene expression of AgRP, NPY, CART and POMC by real-time reverse transcription polymerase chain reaction. In addition, hypothalamic DA, NE and 5-HT levels were measured by high performance liquid chromatography (HPLC) coupled to electrochemical detection. Our findings show that administration of MIA-690 increased food intake and body weight, without affecting locomotor activity. No difference was observed in visceral, subcutaneous and brown fat mass in animals treated with MIA-690 or vehicle. As for neuromodulatory effects, a significant increase of AgRP gene expression and NE levels, along with a reduction of 5-HT levels were found after MIA-690 treatment. On the other hand, we did not observe any alteration in NPY, POMC and CART gene expression, as well as DA levels, following MIA-690 administration. In conclusion, chronic peripheral administration of MIA-690 could play an orexigenic role paralleled by increased body weight. The stimulation of feeding could be mediated, at least in part, by increased AgRP gene expression and NE levels and decreased 5-HT levels, in the hypothalamus.

scholarly journals Quantifying the energy stores of capital breeding humpback whales and income breeding sperm whales using historical whaling records

2017 ◽  
Vol 4 (3) ◽  
pp. 160290 ◽  
Author(s):  
Lyn G. Irvine ◽  
Michele Thums ◽  
Christine E. Hanson ◽  
Clive R. McMahon ◽  
Mark A. Hindell
Keyword(s):  
Sampling Site ◽  
Humpback Whales ◽  
Physeter Macrocephalus ◽  
Sperm Whales ◽  
Body Lipid ◽  
Pregnant Females ◽  
Income Breeding ◽  
Energy Stores ◽  
Total Body ◽  
Lipid Stores

Cetacean energy stores are known to vary according to life history, reproductive status and time of year; however, the opportunity to quantify these relationships is rare. Using a unique set of historical whaling records from Western Australia (1952–1963), we investigated energy stores of large cetaceans with differing life histories, and quantified the relationship between total body lipid and length for humpback whales ( Megaptera novaeangliae) ( n  = 905) and sperm whales (Physeter macrocephalus) ( n  = 1961). We found that total body lipid increased with body length in both humpback and sperm whales, consistent with size-related energy stores. Male humpback whales stored 2.49 kl (15.6 barrels) (31.9–74.9%) more lipid than male sperm whales of equivalent length, to fuel their annual migration. Relative lipid stores of sperm whales (males) were constant throughout the year, while those of humpback whales varied with reproductive class and sampling date. Pregnant female humpback whales had higher relative energy stores than non-pregnant females and males (26.2% and 37.4%, respectively), to fuel the energy demands of gestation and lactation. Those that reached the sampling site later ( en route to their breeding grounds) carried higher lipid stores than those that arrived earlier, possibly reflecting individual variation in residency times in the Antarctic feeding grounds. Importantly, longer pregnant females had relatively larger energy stores than the shorter pregnant females, indicating that the smaller individuals may experience higher levels of energetic stress during the migration fast. The relationships we developed between body lipid and length can be used to inform bioenergetics and ecosystem models when such detailed information is not available.

Comparison of voluntary food intake, apparent digestibility, digesta kinetics and digestive tract content in Manchega and Lacaune dairy sheep in late pregnancy and early and mid lactation

2001 ◽  
Vol 72 (1) ◽  
pp. 209-221 ◽  
Author(s):  
E. Molina ◽  
A. Ferret ◽  
G. Caja ◽  
S. Calsamiglia ◽  
X. Such ◽  
...  
Keyword(s):  
Body Weight ◽  
Food Intake ◽  
Digestive Tract ◽  
Retention Time ◽  
Late Pregnancy ◽  
Apparent Digestibility ◽  
Dairy Sheep ◽  
Mean Retention Time ◽  
Ad Libitum ◽  
Voluntary Food Intake

AbstractTwo experiments were carried out with pregnant (experiment 1) and lactating ewes (experiment 2), to compare dry-matter (DM) intake, and total tract apparent digestibility, digesta kinetics and weight of digestive tract contents of Lacaune and Manchega sheep, with the aim of explaining possible differences between the breeds in voluntary food intake. In experiment 1, 20 3-year-old single-bearing pregnant ewes, 10 Manchega and 10 Lacaune, were permanently housed for the last 10 weeks of pregnancy. The diet used consisted of lucerne hay, offeredad libitum, supplemented with 0·3 kg/day of concentrate. DM and digestible DM intake per kg M0·75were higher (P< 0·01) in Lacaune than in Manchega sheep. Breed did not affect total tract apparent digestibility, fractional rates of passage, transit time, total mean retention time, or weight of digestive tract contents. Changes in body weight and body condition score were similar between breeds. In contrast, Lacaune lambs tended (P= 0·09) to weigh less than Manchega lambs, suggesting genetic differences in the energy utilization between breeds in late pregnancy. Higher DM intake observed in Lacaune sheep may have been related to a higher energy demand for mammary development. In experiment 2, 32 3-year-old lactating multiparous ewes, 16 Manchega and 16 Lacaune, were permanently housed during the first 12 weeks of lactation. The experimental diet used was based on a mixture of maize silage and dehydrated lucerne (10: 1, fresh weight basis), offeredad libitum, and supplemented with 0·8 kg/day of concentrate. Milk, fat and protein yield as well as DM and digestible DM intake in Lacaune ewes was higher (P< 0·01) than in Manchega ewes. DM intake was constant in Lacaune sheep with advancing lactation, while in Manchega sheep DM intake decreased. Throughout this period Lacaune ewes lost 0·5 kg of body weight while Manchega gained 4·4 kg. Breed did not affect either apparent digestibility of DM, organic matter and neutral-detergent fibre, or fractional rates of passage, transit time and total mean retention time. The weight of total tract digestive contents was greater (P< 0·05) in Lacaune than in Manchega sheep, particularly in the reticulo-rumen. Results suggest that the scheme of selection in Lacaune dairy sheep has increased milk yield together with voluntary food intake, the latter being associated with an increase in the rumen fill capacity. The higher milk yield of Lacaune ewes cannot be attributed to the higher DM intake only; other factors, i.e. mobilization of fat reserves, are required to support this higher milk output.

Effects of Cyproterone Acetate on Body Weight and Food Intake

1973 ◽  
Author(s):  
William W. Beatty ◽  
Thomas R. Vilberg ◽  
Paul B. Revland
Keyword(s):  
Body Weight ◽  
Food Intake ◽  
Cyproterone Acetate

FoxO1 ablation in hypothalamic pomc neurons reduces food intake and body weight

2007 ◽  
Vol 2 (S 1) ◽  
Author(s):  
L Plum ◽  
M Matsumoto ◽  
D Accili
Keyword(s):  
Body Weight ◽  
Food Intake

Central Histamine, the H3-Receptor and Obesity Therapy

2019 ◽  
Vol 18 (7) ◽  
pp. 516-522
Author(s):  
Néstor F. Díaz ◽  
Héctor Flores-Herrera ◽  
Guadalupe García-López ◽  
Anayansi Molina-Hernández
Keyword(s):  
Body Weight ◽  
Food Intake ◽  
Animal Studies ◽  
Energy Homeostasis ◽  
Receptor Activation ◽  
Receptor Ligands ◽  
Histaminergic System ◽  
H3 Receptor ◽  
Weight One ◽  
The Central Nervous System

The brain histaminergic system plays a pivotal role in energy homeostasis, through H1- receptor activation, it increases the hypothalamic release of histamine that decreases food intake and reduces body weight. One way to increase the release of hypothalamic histamine is through the use of antagonist/inverse agonist for the H3-receptor. Histamine H3-receptors are auto-receptors and heteroreceptors located on the presynaptic membranes and cell soma of neurons, where they negatively regulate the synthesis and release of histamine and other neurotransmitters in the central nervous system. Although several compounds acting as H3-receptor antagonist/inverse agonists have been developed, conflicting results have been reported and only one has been tested as anti-obesity in humans. Animal studies revealed the opposite effect in food intake, energy expeditor, and body weight, depending on the drug, spice, and route of administration, among others. The present review will explore the state of art on the effects of H3-receptor ligands on appetite and body-weight, going through the following: a brief overview of the circuit involved in the control of food intake and energy homeostasis, the participation of the histaminergic system in food intake and body weight, and the H3-receptor as a potential therapeutic target for obesity.

Roux-en-Y Gastrointestinal Bypass Promotes Activation of TGR5 and Peptide YY

2020 ◽  
Vol 20 (8) ◽  
pp. 1262-1267
Author(s):  
Haojun Yang ◽  
Hanyang Liu ◽  
YuWen Jiao ◽  
Jun Qian
Keyword(s):  
Body Weight ◽  
Food Intake ◽  
Metabolic Diseases ◽  
Peptide Yy ◽  
The Body ◽  
Wild Type ◽  
L Cells ◽  
Body Weights ◽  
Gastrointestinal Bypass ◽  
G Protein Coupled

Background: G protein-coupled bile acid receptor (TGR5) is involved in a number of metabolic diseases. The aim of this study was to identify the role of TGR5 after Roux-en-Y gastric bypass (GBP). Methods: Wild type and TGR5 knockout mice (tgr5-/-) were fed a high-fat diet (HFD) to establish the obesity model. GBP was performed. The changes in body weight and food intake were measured. The levels of TGR5 and peptide YY (PYY) were evaluated by RT-PCR, Western blot, and ELISA. Moreover, the L-cells were separated from wild type and tgr5-/- mice. The levels of PYY in L-cells were evaluated by ELISA. Results: The body weights were significantly decreased after GBP in wild type mice (p<0.05), but not tgr5-/- mice (p>0.05). Food intake was reduced after GBP in wild type mice, but also not significantly affected in tgr5-/- mice (p>0.05). The levels of PYY were significantly increased after GBP compared with the sham group (p<0.05); however, in tgr5-/- mice the expression of PYY was not significantly affected (p>0.05). After INT-777 stimulation in L-cells obtained from murine intestines, the levels of PYY were significantly increased in L-cells tgr5+/+ (p<0.05). Conclusion: Our study suggests that GBP up-regulated the expression of TGR5 in murine intestines, and increased the levels of PYY, which further reduced food intake and decreased the body weight.

Sign in / Sign up

Export Citation Format

Share Document