Cluster analysis tests the importance of myogenic gene expression during myofiber hypertrophy in humans

2007 ◽  
Vol 102 (6) ◽  
pp. 2232-2239 ◽  
Author(s):  
Marcas M. Bamman ◽  
John K. Petrella ◽  
Jeong-su Kim ◽  
David L. Mayhew ◽  
James M. Cross
Keyword(s):  
Cluster Analysis ◽  
Human Subjects ◽  
Cell Activity ◽  
Differentially Expressed ◽  
Entire Cohort ◽  
Specific Factors ◽  
First Time ◽  
Muscle Biopsies ◽  
Satellite Cell Activity

We applied K-means cluster analysis to test the hypothesis that muscle-specific factors known to modulate protein synthesis and satellite cell activity would be differentially expressed during progressive resistance training (PRT, 16 wk) in 66 human subjects experiencing extreme, modest, and failed myofiber hypertrophy. Muscle mRNA expression of IGF-I isoform Ea (IGF-IEa), mechanogrowth factor (MGF, IGF-IEc), myogenin, and MyoD were assessed in muscle biopsies collected at baseline (T1) and 24 h after the first (T2) and last (T3) loading bouts from previously untrained subjects clustered as extreme responders (Xtr, n = 17), modest responders (Mod, n = 32), and nonresponders (Non, n = 17) based on mean myofiber hypertrophy. Myofiber growth averaged 2,475 μm2 in Xtr, 1,111 μm2 in Mod, and −16 μm2 in Non. Main training effects revealed increases in all transcripts (46–83%, P < 0.005). For the entire cohort, IGF-IEa, MGF, and myogenin mRNAs were upregulated by T2 ( P < 0.05), while MyoD did not increase significantly until T3 ( P < 0.001). Within clusters, MGF and myogenin upregulation was robust in Xtr (126% and 65%) and Mod (73% and 41%) vs. no changes in Non. While significant in all clusters by T3, IGF-IEa increased most in Xtr (105%) and least in Non (44%). Although MyoD expression increased overall, no changes within clusters were detected. We reveal for the first time that MGF and myogenin transcripts are differentially expressed in subjects experiencing varying degrees of PRT-mediated myofiber hypertrophy. The data strongly suggest the load-mediated induction of these genes may initiate important actions necessary to promote myofiber growth during PRT, while the role of MyoD is less clear.

Immunity to an attenuated variant ofPlasmodium berghei: role of some non-specific factors

Parasitology ◽  
1985 ◽  
Vol 91 (2) ◽  
pp. 263-272 ◽  
Author(s):  
Seiji waki ◽  
Shushke Nakazawa ◽  
Janice Taverne ◽  
G. A. T. Targett ◽  
J. H. L. Playfair
Keyword(s):  
Plasmodium Berghei ◽  
Tumour Necrosis ◽  
Nk Cell ◽  
Cell Activity ◽  
Nk Cell Activity ◽  
Parasite Number ◽  
Early Peak ◽  
Specific Factors ◽  
Increased Susceptibility

Plasmodium bergheiXAT, an attenuated variant of lethalP. berghei, causes a resolving infection in Balb/c mice from which they recover in about 3 weeks. The parasitaemia displays an early peak at about 5 days, followed by a steep drop in parasite number associated with the appearance of degenerating forms inside mature erythrocytes; the parasites remaining are inside reticulocytes. By contrast, no degenerating parasites were seen in infections caused by the virulent parent, which was mainly confined to mature erythrocytes. However,P. bergheiXAT was no more sensitive to reactive O2metabolites, generated by alloxan, or to tumour necrosis serum, than its virulent parent. Furthermore, its early drop in parasitaemia was unaffected by silica. The drop still occurred in the absence of T cells, although the infection was then ultimately lethal, and it was not mediated by NK cells since it occurred in nude mice treated with anti-asialo GM1 serum to abolish NK cell activity. However, it was absent in splenectomized mice, in whichP. bergheiXAT infection was lethal. Thus, the attenuation ofP. bergheiXAT infection is not due to increased susceptibility to some of the agents thought to cause parasite destruction, but to some other mechanism in which the spleen is involved.

The influence of anti-inflammatory medication on exercise-induced myogenic precursor cell responses in humans

2007 ◽  
Vol 103 (2) ◽  
pp. 425-431 ◽  
Author(s):  
Abigail L. Mackey ◽  
Michael Kjaer ◽  
Sune Dandanell ◽  
Kristian H. Mikkelsen ◽  
Lars Holm ◽  
...  
Keyword(s):  
Satellite Cell ◽  
Cell Activity ◽  
Cell Number ◽  
Peak Oxygen Consumption ◽  
Neural Cell Adhesion ◽  
Inflammatory Drugs ◽  
Anti Inflammatory ◽  
Exercise Induced ◽  
Muscle Biopsies ◽  
Satellite Cell Activity

The consumption of nonsteroidal anti-inflammatory drugs (NSAIDs) is widespread among athletes when faced with muscle soreness or injury, but the effects of NSAIDs on satellite cell activity in humans are unknown. To investigate this, 14 healthy male endurance athletes (mean peak oxygen consumption 62 ml·kg−1·min−1) volunteered for the study, which involved running 36 km. They were divided into two groups and received either 100 mg indomethacin per day or placebo. Muscle biopsies collected before the run and on days 1, 3, and 8 afterward were analyzed for satellite cells by immunohistochemistry with the aid of neural cell adhesion molecule (NCAM) and fetal antigen-1 (FA1) antibodies. Muscle biopsies were also collected from untrained individuals for comparison. Compared with preexercise levels, a 27% increase in the number of NCAM+ cells was observed on day 8 postexercise in the placebo group ( P < 0.05), while levels remained similar at all time points in the NSAID group. No change was seen in the proportion of FA1+ cells, although lower levels were found in the muscle of endurance-trained athletes compared with untrained individuals ( P < 0.05). These results suggest that ingestion of anti-inflammatory drugs attenuates the exercise-induced increase in satellite cell number, supporting the role of the cyclooxygenase pathway in satellite cell activity.

scholarly journals Loss of Ptpn11 (Shp2) drives satellite cells into quiescence

eLife ◽  
2017 ◽  
Vol 6 ◽  
Author(s):  
Joscha Griger ◽  
Robin Schneider ◽  
Ines Lahmann ◽  
Verena Schöwel ◽  
Charles Keller ◽  
...  
Keyword(s):  
Stem Cells ◽  
Tyrosine Phosphatase ◽  
Muscle Growth ◽  
Cell Activity ◽  
Muscle Stem Cells ◽  
Myogenic Stem Cells ◽  
Postnatal Myogenesis ◽  
Receptor Tyrosine Phosphatase ◽  
Satellite Cell Activity

The equilibrium between proliferation and quiescence of myogenic progenitor and stem cells is tightly regulated to ensure appropriate skeletal muscle growth and repair. The non-receptor tyrosine phosphatase Ptpn11 (Shp2) is an important transducer of growth factor and cytokine signals. Here we combined complex genetic analyses, biochemical studies and pharmacological interference to demonstrate a central role of Ptpn11 in postnatal myogenesis of mice. Loss of Ptpn11 drove muscle stem cells out of the proliferative and into a resting state during muscle growth. This Ptpn11 function was observed in postnatal but not fetal myogenic stem cells. Furthermore, muscle repair was severely perturbed when Ptpn11 was ablated in stem cells due to a deficit in stem cell proliferation and survival. Our data demonstrate a molecular difference in the control of cell cycle withdrawal in fetal and postnatal myogenic stem cells, and assign to Ptpn11 signaling a key function in satellite cell activity.

scholarly journals Differential microRNAs expression profiles in liver from three different lifestyle modification mice models

2020 ◽  
Author(s):  
Huan Gong ◽  
Ming Zhang ◽  
Yiwen Han ◽  
Ying Zhang ◽  
Jing Pang ◽  
...  
Keyword(s):  
High Fat Diet ◽  
Target Genes ◽  
Lifestyle Modification ◽  
Expression Profiles ◽  
Differentially Expressed ◽  
Lifestyle Modifications ◽  
Enriched Categories ◽  
The Common ◽  
First Time

Abstract Background MicroRNAs play an important role in many fundamental biological and pathological processes. Defining the microRNAs profile underlying the processes by beneficial and detrimental lifestyles, including caloric restriction(CR), exercise and high-fat diet(HF), is necessary for understanding both normal physiology and the pathogenesis of metabolic disease. We used the microarray to detect microRNAs expression in livers from CR, EX and HF mice models. After predicted potential target genes of differentially expressed microRNAs with four algorithms, we applied GO and KEGG to analyze the function of predicted microRNA targets. Results We describe the overall microRNAs expression pattern, and identified 84 differentially expressed microRNAs changed by one or two or even all the three lifestyle modifications. The common and different enriched categories of gene function and main biochemical and signal transduction pathways were presented. Conclusions We provided for the first time a comprehensive and thorough comparison of microRNAs expression profiles in liver among these lifestyle modifications. With this knowledge, our findings provide us with an overall vision of microRNAs in the molecular impact of lifestyle on health as well as useful clues for future and thorough research of the role of microRNAs.

scholarly journals Differential Expression of Long Noncoding RNAs Reveals a Potential Biomarker for Intractable Pemphigus

2021 ◽  
Vol 2021 ◽  
pp. 1-14
Author(s):  
Peng Qu ◽  
Zixuan Huang ◽  
Haiqin Zhu ◽  
Jie Zheng ◽  
Meng Pan
Keyword(s):  
Expression Profile ◽  
Operating Characteristic ◽  
Mononuclear Cells ◽  
Noncoding Rnas ◽  
Long Noncoding Rnas ◽  
Differentially Expressed ◽  
Peripheral Blood Mononuclear ◽  
Potential Biomarker ◽  
First Time

Background. Long noncoding RNAs (lncRNAs) are involved in autoimmune diseases. However, the role of lncRNAs in pemphigus remains elusive. Objective. The study is aimed at investigating the expression profile in pemphigus patients to identify a circulating lncRNA as a novel biomarker for pemphigus. Method. A global lncRNA expression profile in peripheral blood mononuclear cells (PBMCs) was measured by lncRNA microarray. Differentially expressed lncRNAs were validated by quantitative reverse transcriptase-PCR (qRT-PCR). The functional and biological processes of the differentially expressed lncRNAs were analyzed by bioinformatics. Results. lncRNA ENST00000585297 in the PBMCs of pemphigus patients was highly expressed compared with those of HCs and BP patients. The area under the receiver operating characteristic (ROC) curve was 0.846 ( 95 % confidence   interval   CI = 0.7526 to 0.9397). Intriguingly, we found that the expression of ENST00000585297 was upregulated in pemphigus patients whose symptoms could not be managed within four weeks compared to other patients whose symptoms could be managed in four weeks or less ( P < 0.05 ). In addition, ENST00000585297 expression in pemphigus patients was positively correlated with the dosage of prednisone needed to manage the disorder ( r = 0.4905 , P = 0.0094 ). A competing endogenous RNA (ceRNA) regulatory network was constructed based on the ceRNA theory. Further verification demonstrated that silencing of ENST00000585297 increased the expression of miR-584-3p. Conclusions. Our study revealed for the first time the expression profile of lncRNAs in pemphigus patients. In addition, our study identified ENST00000585297 as a biomarker and indicator for the intractable course of pemphigus.

scholarly journals N7-methylguanosine modification of lncRNAs in a rat model of hypoxic pulmonary hypertension: a comprehensive analysis

BMC Genomics ◽  
2022 ◽  
Vol 23 (1) ◽  
Author(s):  
Huan Wang ◽  
Ren Biao Chen ◽  
Si Ni Zhang ◽  
Rui Feng Zhang
Keyword(s):  
Pulmonary Hypertension ◽  
Clinical Significance ◽  
Rat Model ◽  
Critical Role ◽  
Comprehensive Analysis ◽  
Differentially Expressed ◽  
Hypoxic Pulmonary Hypertension ◽  
Non Coding Rnas ◽  
First Time

Abstract Background Long non-coding RNAs (lncRNAs) play a critical role in the pathogenesis of hypoxic pulmonary hypertension (HPH). The role of N7-methylguanosine (m7G) modification in lncRNAs has received increased attentions in recent years. However, the m7G-methylation of lncRNA in HPH has yet to be determined. We have therefore performed a transcriptome-wide analysis of m7G lncRNAs in HPH. Results Differentially-expressed m7Gs were detected in HPH, and m7G lncRNAs were significantly upregulated compared with non-m7G lncRNAs in HPH. Importantly, this was the first time that the upregulated m7G lncXR_591973 and m7G lncXR_592398 were identified in HPH. Conclusion This study provides the first m7G transcriptome-wide analysis of HPH. Importantly, two HPH-associated m7G lncRNAs were identified, although their clinical significance requires further validation.

scholarly journals Differential microRNAs expression profiles in liver from three different lifestyle modification mice models

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Huan Gong ◽  
Ming Zhang ◽  
Yiwen Han ◽  
Ying Zhang ◽  
Jing Pang ◽  
...  
Keyword(s):  
High Fat Diet ◽  
Target Genes ◽  
Lifestyle Modification ◽  
Expression Profiles ◽  
Differentially Expressed ◽  
Lifestyle Modifications ◽  
Enriched Categories ◽  
The Common ◽  
First Time

Abstract Background MicroRNAs play an important role in many fundamental biological and pathological processes. Defining the microRNAs profile underlying the processes by beneficial and detrimental lifestyles, including caloric restriction (CR), exercise and high-fat diet (HF), is necessary for understanding both normal physiology and the pathogenesis of metabolic disease. We used the microarray to detect microRNAs expression in livers from CR, EX and HF mice models. After predicted potential target genes of differentially expressed microRNAs with four algorithms, we applied GO and KEGG to analyze the function of predicted microRNA targets. Results We describe the overall microRNAs expression pattern, and identified 84 differentially expressed microRNAs changed by one or two or even all the three lifestyle modifications. The common and different enriched categories of gene function and main biochemical and signal transduction pathways were presented. Conclusions We provided for the first time a comprehensive and thorough comparison of microRNAs expression profiles in liver among these lifestyle modifications. With this knowledge, our findings provide us with an overall vision of microRNAs in the molecular impact of lifestyle on health as well as useful clues for future and thorough research of the role of microRNAs.

scholarly journals Differential microRNAs expression profiles in liver from three different lifestyle modification mice models

2020 ◽  
Author(s):  
Huan Gong ◽  
Ming Zhang ◽  
Yiwen Han ◽  
Ying Zhang ◽  
Jing Pang ◽  
...  
Keyword(s):  
High Fat Diet ◽  
Target Genes ◽  
Lifestyle Modification ◽  
Expression Profiles ◽  
Differentially Expressed ◽  
Lifestyle Modifications ◽  
Enriched Categories ◽  
The Common ◽  
First Time

Abstract Background MicroRNAs play an important role in many fundamental biological and pathological processes. Defining the microRNAs profile underlying the processes by beneficial and detrimental lifestyles, including caloric restriction(CR), exercise and high-fat diet(HF), is necessary for understanding both normal physiology and the pathogenesis of metabolic disease. We used the microarray to detect microRNAs expression in livers from CR, EX and HF mice models. After predicted potential target genes of differentially expressed microRNAs with four algorithms, we applied GO and KEGG to analyze the function of predicted microRNA targets. Results We describe the overall microRNAs expression pattern, and identified 84 differentially expressed microRNAs changed by one or two or even all the three lifestyle modifications. The common and different enriched categories of gene function and main biochemical and signal transduction pathways were presented. Conclusions We provided for the first time a comprehensive and thorough comparison of microRNAs expression profiles in liver among these lifestyle modifications.With this knowledge, our findings provide us with an overall vision of microRNAs in the molecular impact of lifestyle on health as well as useful clues for future and thorough research of the role of microRNAs.

Psychological Resilience Provides No Independent Protection From Suicidal Risk

Crisis ◽  
2016 ◽  
Vol 37 (2) ◽  
pp. 130-139 ◽  
Author(s):  
Danica W. Y. Liu ◽  
A. Kate Fairweather-Schmidt ◽  
Richard Burns ◽  
Rachel M. Roberts ◽  
Kaarin J. Anstey
Keyword(s):  
Risk Factors ◽  
Well Being ◽  
Current Status ◽  
Psychological Resilience ◽  
Cross Sectional ◽  
Life Project ◽  
Resilience Factors ◽  
First Time

Abstract. Background: Little is known about the role of resilience in the likelihood of suicidal ideation (SI) over time. Aims: We examined the association between resilience and SI in a young-adult cohort over 4 years. Our objectives were to determine whether resilience was associated with SI at follow-up or, conversely, whether SI was associated with lowered resilience at follow-up. Method: Participants were selected from the Personality and Total Health (PATH) Through Life Project from Canberra and Queanbeyan, Australia, aged 28–32 years at the first time point and 32–36 at the second. Multinomial, linear, and binary regression analyses explored the association between resilience and SI over two time points. Models were adjusted for suicidality risk factors. Results: While unadjusted analyses identified associations between resilience and SI, these effects were fully explained by the inclusion of other suicidality risk factors. Conclusion: Despite strong cross-sectional associations, resilience and SI appear to be unrelated in a longitudinal context, once risk/resilience factors are controlled for. As independent indicators of psychological well-being, suicidality and resilience are essential if current status is to be captured. However, the addition of other factors (e.g., support, mastery) makes this association tenuous. Consequently, resilience per se may not be protective of SI.

Conditional Giving: The Role of Conflict Specific Factors on Willingness to Sacrifice

2006 ◽  
Author(s):  
Brent A. Mattingly ◽  
Eddie M. Clark ◽  
Kiara J. Weaver ◽  
Tim M. Emge ◽  
Chris K. Adair
Keyword(s):  
Specific Factors ◽  
Willingness To Sacrifice
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