scholarly journals Interaction of porphyrin/oligonucleotide complex with liposomes studied by drop coating deposition Raman spectroscopy

2010 ◽  
Vol 24 (3-4) ◽  
pp. 197-200 ◽  
Author(s):  
E. Kocišová ◽  
M. Procházka ◽  
J. Štepánek ◽  
P. Mojzeš
Keyword(s):  
Raman Spectroscopy ◽  
Lipid Composition ◽  
Hydrophobic Surface ◽  
Small Species ◽  
Drying Process ◽  
High Quality ◽  
Coating Deposition ◽  
Solution Sample ◽  
Plasmatic Membrane ◽  
Edge Part

Drop coating deposition Raman (DCDR) microspectroscopy was used to investigate interaction of the complexed cationic copper 5,10,15,20-tetrakis(1-methyl-4-pyridyl) porphyrin (CuP) and phosphorothioate analog of dT15oligonucleotide with liposomes, the lipid composition of which imitated the natural plasmatic membrane. Great advantage of dried drops on DCDR plates over a solution sample is that the specific drying process on the special hydrophobic surface efficiently separates liposomes from small species in the solvent. In our case, liposomes with bound CuP/oligonucleotide complexes formed a ring at the edge part of the dried drop while dried solution of this complex remained inside this ring. High quality spectra measured from the ring by using Raman confocal microspectrometer revealed unperturbed arrangement of lipid chains by the drying process, partial binding of the CuP/oligonucleotide complexes to liposomes, and a certain reorientation of lipid chains as a consequence of this interaction.

scholarly journals DCDR Spectroscopy as Efficient Tool for Liposome Studies: Aspect of Preparation Procedure Parameters

2012 ◽  
Vol 27 ◽  
pp. 349-353 ◽  
Author(s):  
E. Kočišová ◽  
A. Vodáková ◽  
M. Procházka
Keyword(s):  
Lipid Composition ◽  
Phosphate Buffer ◽  
Initial Phase ◽  
Liquid Crystalline ◽  
Phase State ◽  
Hydrophobic Surface ◽  
Drying Process ◽  
Lipid Concentration ◽  
Efficient Tool ◽  
Coating Deposition

Drop-coating deposition Raman (DCDR) spectroscopy was employed to study liposome suspensions. The method is based on a specific drying process on the hydrophobic surface that efficiently accumulates the macromolecular sample in a ring of the edge of the dried drop. We studied liposome suspensions purchased from two sources (Avanti Polar Lipids, Inc. and Sigma-Aldrich, Co.) and prepared under different conditions. Structure of the dried drop substantially depends on the lipid concentration, lipid composition of the sample, and used solvent. Optimal lipid concentration is about 0.3 mg/ml in all cases, asolectin and DSPC suspensions form compact dried drops when dissolved in water and phosphate buffer, respectively. Drying process of the sample drop does not influence the initial phase state (gel or liquid-crystalline) of the studied liposomes excepting DSPC from Sigma-Aldrich, Co.

scholarly journals Flux Method Growth of Large Size Group IV–V 2D GeP Single Crystals and Photoresponse Application

Crystals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 235
Author(s):  
Shuqi Zhao ◽  
Tongtong Yu ◽  
Ziming Wang ◽  
Shilei Wang ◽  
Limei Wei ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Single Crystals ◽  
High Performance ◽  
Group Iv ◽  
X Ray Diffraction ◽  
High Quality ◽  
Practical Applications ◽  
Large Size ◽  
Growth Method ◽  
Polarized Raman

Two-dimensional (2D) materials driven by their unique electronic and optoelectronic properties have opened up possibilities for their various applications. The large and high-quality single crystals are essential to fabricate high-performance 2D devices for practical applications. Herein, IV-V 2D GeP single crystals with high-quality and large size of 20 × 15 × 5 mm3 were successfully grown by the Bi flux growth method. The crystalline quality of GeP was confirmed by high-resolution X-ray diffraction (HRXRD), Laue diffraction, electron probe microanalysis (EPMA) and Raman spectroscopy. Additionally, intrinsic anisotropic optical properties were investigated by angle-resolved polarized Raman spectroscopy (ARPRS) and transmission spectra in detail. Furthermore, we fabricated high-performance photodetectors based on GeP, presenting a relatively large photocurrent over 3 mA. More generally, our results will significantly contribute the GeP crystal to the wide optoelectronic applications.

scholarly journals Vibrational Spectroscopic Investigation of Blood Plasma and Serum by Drop Coating Deposition for Clinical Application

2021 ◽  
Vol 22 (4) ◽  
pp. 2191
Author(s):  
Jing Huang ◽  
Nairveen Ali ◽  
Elsie Quansah ◽  
Shuxia Guo ◽  
Michel Noutsias ◽  
...  
Keyword(s):  
Ir Spectroscopy ◽  
Outer Ring ◽  
Imaging Method ◽  
Fluorescence Lifetime Imaging ◽  
Drying Process ◽  
Vibrational Signal ◽  
Coating Deposition ◽  
Ft Ir ◽  
Sample Droplet ◽  
Ft Ir Spectroscopy

In recent decades, vibrational spectroscopic methods such as Raman and FT-IR spectroscopy are widely applied to investigate plasma and serum samples. These methods are combined with drop coating deposition techniques to pre-concentrate the biomolecules in the dried droplet to improve the detected vibrational signal. However, most often encountered challenge is the inhomogeneous redistribution of biomolecules due to the coffee-ring effect. In this study, the variation in biomolecule distribution within the dried-sample droplet has been investigated using Raman and FT-IR spectroscopy and fluorescence lifetime imaging method. The plasma-sample from healthy donors were investigated to show the spectral differences between the inner and outer-ring region of the dried-sample droplet. Further, the preferred location of deposition of the most abundant protein albumin in the blood during the drying process of the plasma has been illustrated by using deuterated albumin. Subsequently, two patients with different cardiac-related diseases were investigated exemplarily to illustrate the variation in the pattern of plasma and serum biomolecule distribution during the drying process and its impact on patient-stratification. The study shows that a uniform sampling position of the droplet, both at the inner and the outer ring, is necessary for thorough clinical characterization of the patient’s plasma and serum sample using vibrational spectroscopy.

Drop-coating deposition Raman spectroscopy of liposomes

2011 ◽  
Vol 42 (8) ◽  
pp. 1606-1610 ◽  
Author(s):  
Eva Kočišová ◽  
Marek Procházka
Keyword(s):  
Raman Spectroscopy ◽  
Coating Deposition

Surface Amplification of Tetraphenylporphyrin Overtone and Combination Raman Bands in Drop Coating Deposition Raman (DCDR) on Electrically Conductive Surfaces

2021 ◽  
Author(s):  
Phutri Milana ◽  
Veinardi Suendo ◽  
Tika Pebriani ◽  
Fry Voni Steky ◽  
Didi Prasetyo Benu ◽  
...  
Keyword(s):  
Measurement Technique ◽  
Molecular Level ◽  
Reliable Data ◽  
High Quality ◽  
Electrically Conductive ◽  
Coating Deposition ◽  
Raman Measurement ◽  
Level Analysis ◽  
Conductive Surfaces

It is essential to realize a Raman measurement technique without artifact or fluorescence signals for high-quality and reliable data in a valid molecular-level analysis and interpretation. This requirement applies especially...

scholarly journals Quality of graphene on sapphire: long-range order from helium diffraction versus lattice defects from Raman spectroscopy

RSC Advances ◽  
2016 ◽  
Vol 6 (25) ◽  
pp. 21235-21245 ◽  
Author(s):  
Gloria Anemone ◽  
Esteban Climent-Pascual ◽  
Hak Ki Yu ◽  
Amjad Al Taleb ◽  
Felix Jiménez-Villacorta ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Long Range ◽  
Range Order ◽  
Lattice Defects ◽  
New Method ◽  
Long Range Order ◽  
High Quality

We report a new method to produce high-quality, transparent graphene/sapphire samples, using Cu as a catalyst.

scholarly journals Preparation of BaZrO3 powders by a spray-drying process

2003 ◽  
Vol 18 (6) ◽  
pp. 1325-1332 ◽  
Author(s):  
B. Robertz ◽  
F. Boschini ◽  
A. Rulmont ◽  
R. Cloots ◽  
I. Vandriessche ◽  
...  
Keyword(s):  
Spray Drying ◽  
Precursor Solution ◽  
Barium Zirconate ◽  
Drying Process ◽  
X Ray Diffraction ◽  
High Quality ◽  
Corrosion Resistant ◽  
X Ray ◽  
Potential Use ◽  
Scanning Electron

The potential use of barium zirconate for the manufacture of corrosion-resistant substrates emphasizes the need for a simple, inexpensive, and easily scalable process to produce high-quality powders with well-controlled composition and properties. However, the classical solid-state preparation of barium zirconate leads to an inhomogeneous powder unsuitable for applications in highly corrosive environment. For this paper, the possibility to use the spray-drying technique for the preparation of BaZrO3 powders with a controlled size distribution and morphology was investigated. The influence of the nature and concentration of the precursor solution and the influence of the spray-drying step are discussed on the basis of x-ray diffraction, Fourier transform infrared spectroscopy, scanning electron microscopy, and dilatometric measurements.

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