scholarly journals Effects of Exogenous Corticosterone on Circulating Leukocytes of a Salamander (Ambystoma talpoideum) with Unusually Abundant Eosinophils

2010 ◽  
Vol 2010 ◽  
pp. 1-8 ◽  
Author(s):  
Andrew K. Davis ◽  
John C. Maerz
Keyword(s):  
Blood Cells ◽  
Stress Hormones ◽  
White Blood Cells ◽  
Glucocorticoid Hormones ◽  
Control Groups ◽  
Sham Injection ◽  
Blood Smears ◽  
Ambystoma Talpoideum ◽  
Leukocyte Counts ◽  
And Control

When animals become stressed, their levels of glucocorticoid hormones increase, causing white blood cells to move from tissues to circulation or vice versa. The primary alteration is an increase in the abundance of circulating neutrophils and a decrease in lymphocytes in circulation. A lesser-known effect is a decrease in the number of circulating eosinophils. Salamanders in the genusAmbystomahave unusually high numbers of circulating eosinophils, and as such, any effect of stress hormones on circulating leukocytes (especially eosinophils) of these species should be especially pronounced. We conducted an experiment to determine the effect of corticosterone administration on leukocyte counts (from blood smears) ofA. talpoideumsalamanders. Salamanders were captured and sampled as reference animals(n=11), given a sham injection(n=8), or injected with 0.1cc of a 100 μg/mL corticosterone solution(n=28). After 24 hours, relative neutrophil counts were higher and relative lymphocyte counts lower, in the corticosterone group than the sham and control groups. Absolute counts showed that this effect was driven by a reduction in lymphocytes, since neutrophil counts were statistically similar across treatments. Importantly, relative and absolute numbers of eosinophils decreased in the sham and corticosterone groups, confirming the sensitivity of this cell to stress in amphibians.

scholarly journals The utility of basic blood counts, WBC histogram and C-reactive protein in detecting malaria

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Jun Nishimura ◽  
Parag Dharap ◽  
Sebastien Raimbault
Keyword(s):  
Blood Cells ◽  
Malaria Infection ◽  
Monsoon Season ◽  
Characteristic Curve ◽  
White Blood Cells ◽  
C Reactive Protein ◽  
Control Groups ◽  
Reactive Protein ◽  
Hematology Analyzer ◽  
And Control

Abstract Background Hematology analyzers display abnormal parameters during malaria infection providing insightful information for suspecting and assessing malaria infection. The goal of this study is to demonstrate the potential of a three-part differential hematology analyzer to assess malaria, provide information about the parasitemia, and discuss the importance of combining C-reactive protein (CRP) with hematology parameters to obtain further information about the malaria infection. Methods The present study shows the results of a case–control study during the monsoon season of years 2018 and 2019 in Mumbai, India. The study considers 1008 non-malaria febrile cases, 209 P. vivax and 31 P. falciparum positive malaria samples, five cases of mixed P. vivax and P. falciparum infection, and three co-infection cases of P. vivax and dengue. Raw data from the three-part analyzer LC-667G CRP (HORIBA) and the corresponding microscopic findings (golden standard for diagnosis of malaria) were obtained for each sample. Results The medians of platelet counts (PLT) were 102.5, 109.0, and 223.0 × 103/µL, while CRP medians were 67.4, 81.4 and 10.4 mg/L in P. vivax, P. falciparum and control groups respectively (p < 0.001 in Mann–Whitney U tests between malaria and control groups). Compared with negative samples, platelets counting less than 161.5 × 103/µL were observed on malaria patients (OR 19.12, 95% CI 11.89–30.75). Especially in P. vivax cases, an abnormal peak was frequently observed in the white blood cells (WBC) histogram around the 37fL channel. The events counted around that channel showed a linear correlation with the counting of red blood cells infected predominantly with larger parasitic forms. Parameters like CRP (rs = 0.325, p < 0.001), WBC (rs = 0.285, p < 0.001) and PLT (rs = − 0.303, p < 0.001) were correlated with the parasitemia of P. vivax samples. Between the malaria and dengue groups, the highest area under the receiver operating characteristic curve was observed on CRP (0.867, CRP ≥ 26.85 mg/L). Conclusions A three-part differential hematology analyzer has the potential to not only trigger malaria diagnosis confirmation but also assess the severity of the infection when CRP is considered.

scholarly journals Effects of diet supplementation with different level of Celmanax® in rainbow trout (Oncorhyncus mykiss)

2019 ◽  
Vol 70 (1) ◽  
pp. 1347
Author(s):  
A. KHODADADI ◽  
A. HAGHIGHI ◽  
H. MALEKINEJADH ◽  
A. TUKMECHI ◽  
M. AFSHARNASAB
Keyword(s):  
Rainbow Trout ◽  
Neutrophil Count ◽  
Blood Cells ◽  
Biochemical Parameters ◽  
White Blood Cells ◽  
Haemoglobin Concentration ◽  
Serum Biochemical Parameters ◽  
Control Groups ◽  
Serum Biochemical ◽  
And Control

The aim of this study was to evaluate the effect of a prebiotic (Celmanax®) containing yeast cell wall with mannan oligosaccharides on the haematological and serum biochemical parameters in rainbow trout. Three levels of prebiotic (0, 0.1, 0.5 and 1 %) were mixed into pellets. Fish (19.08±1.45 g) were fed a supplemented commercial diet for 60 days. Blood samples were colected from the onset and on days 30 and 60 of the trial to measure the haematological and serum biochemical parameters in rainbow trout. The results showed significant differences in haemoglobin, mean corpuscular volume,mean corpuscular haemoglobin concentration, white blood cells and neutrophil count between control and all test groups (p<0.05). The highest and the lowest white blood cells and neutrophil count (on day 60) were observed in the 0.1 %, prebiotic-received and control groups, respectively. Also, the result showed significant differences in Alkaline phosphatase enzymes, serum glutamic oxaloacetic transaminase, Serum glutamic-pyruvic transaminase, between the test and control groups(p<0.05) while non-significant elevation of blood urea nitrogen, creatinine and total protein levels was found in the Celmanax®-received groups (p>0.05). These results suggest that the Celmanax® supplementation enhances white blood cells and neutrophil count, and changes some biochemical parameters in rainbow trout.

scholarly journals White Blood Cell Image Classification Using Deep Learning

2021 ◽  
Vol 7 (09) ◽  
pp. 7-12
Author(s):  
Neerukattu Indrani and Chiraparapu Srinivasa Rao
Keyword(s):  
Deep Learning ◽  
Blood Cells ◽  
White Blood Cells ◽  
Training Phase ◽  
Counting System ◽  
Cell Image ◽  
Area Of Interest ◽  
Manual Counting ◽  
Blood Smears ◽  
Microscopic Inspection

The microscopic inspection of blood smears provides diagnostic information concerning patients’ health status. For example, the presence of infections, leukemia, and some particular kinds of cancers can be diagnosed based on the results of the classification and the count of white blood cells. The traditional method for the differential blood count is performed by experienced operators. They use a microscope and count the percentage of the occurrence of each type of cell counted within an area of interest in smears. Obviously, this manual counting process is very tedious and slow. In addition, the cell classification and counting accuracy may depend on the capabilities and experiences of the operators. Therefore, the necessity of an automated differential counting system becomes inevitable. In this paper, CNN models are used. In order to achieve good performance from deep learning methods, the network needs to be trained with large amounts of data during the training phase. We take the images of the white blood cells for the training phase and train our model on them. With this method we achieved good accuracy than traditional methods. And we can generate the results within the seconds also.

scholarly journals Study the Oxidative Stress, Some Biochemical and Hematological Parameters in patients with agout at AL-Ramadi city

2016 ◽  
Vol 13 (4) ◽  
pp. 694-701
Author(s):  
Baghdad Science Journal
Keyword(s):  
Blood Cells ◽  
Hematological Parameters ◽  
Age Groups ◽  
Control Group ◽  
Enzymatic Antioxidants ◽  
Blood Picture ◽  
Control Groups ◽  
Blood Samples ◽  
Normal Individuals ◽  
And Control

This study aims to study the effect of gout disease on complete blood picture and biochemical parameters and some non-enzymatic antioxidants, some tracing elements and lipid peroxidation ,in outpatients with gout disease at Al-Ramadi Teaching-Hospital ,Al-Razi Hospital and the study duration from Octo.2013-to May 2014.(50) blood samples were collected from patients with age groups (30-80 years) from both sexes (28 males,22 females),a (30) blood samples (15 males,15 females) were collected from normal individuals as a control group with age groups (27-75 years). Hematological measurement showed no significant differences in size compressed blood cells, the percentages in ( 45.15 +4.99 and 46.87+6.30) % in patient and control groups respectively, hemoglobin concentrations were ( 14.04+1.66 and 14.30+1.93) g/l in patient and control groups respectively, total number of red blood cells ( 5.21+0.43 and 5.12 +0.58) 106/mm3 in patient and control groups respectively with(P?0.05) in ESR (21.06+13.47 and 13.37 +7.45) mm/hr in patient and control groups respectively with (P?0.05), the total number of WBCs were recorded (8.96+2.04 and 7.50+1.69)in patient and control groups respectively. Results showed also significant differences (P?0.05) in uric acid levels (7.42+0.76 and 5.62+0.88) mg/dl,malondialdehyde levels were recorded (4.45+0.64 and 3.21+0.86) in patient and control groups

scholarly journals Effects of Fruits of Lapsi (Nepali Hog Plum) Choerospondias axillaris (roxb.) b.l.Burtt & a.w.Hill on Some Blood Parameters of Major Carp rohu Labeo rohita(H) During Intensive Aquaculture

2020 ◽  
Vol 19 (1) ◽  
pp. 186-195
Author(s):  
Shubha Ratna Shakya ◽  
Shyam Narayan Labh
Keyword(s):  
Blood Cells ◽  
Labeo Rohita ◽  
Control Diet ◽  
White Blood Cells ◽  
Ethanol Extract ◽  
Blood Parameters ◽  
Significant Difference ◽  
Fish Feeds ◽  
Intensive Aquaculture ◽  
And Control

 The complete blood cell count (CBC) is an important and powerful diagnostic tool to monitor the health status of fish in response to changes related to nutrition, water quality, and disease in response to therapy.Thus, the present study was about to know the effect of lapsi fruit pulp (Choerospondias axillaris) on some blood parameters in the fingerlings of rohu Labeo rohita cultured in Corona of Agriculture Hatchery farm, Chitwan, Nepal. Altogether eighteen hapas made of heavy-duty nylon net (1.5m3) were kept and placed inside the pond, distributed linearly, and then 270 fingerlings (@15 fishes/ hapa) were kept distributed randomly. Six practical diets like T1 (0.0 g kg-1), T2 (0.1 g kg-1), T3 (0.2 g kg-1), T4 (0.4 g kg-1), T5 (0.8 g kg-1) and T6 (1.6 g kg-1) were prepared. Feed containing 40% protein was supplemented with the ethanol extract of lapsi fruits. At the end of 90 days of the feeding trial, a significant difference (P< 0.05) in blood parameters were observed between the treated and control diet-fed groups. Hemoglobin (Hb), white blood cells (WBCs), red blood cells (RBCs), packed cell volume (PCV), and other erythrocyte indices were recorded higher in the treated groups. RBC, WBC, Hct, and Hb were found significantly higher in the T4 (0.4 %) diet-fed group. The study showed a minimum of 0.4 % (0.4g kg-1) lapsi fruit extract needed in fish feeds to increase blood parameters to enhance growth and immunity.

Reference Values for Selected Blood Parameters in Rabbits: Effects of Age and Physiological Status

2019 ◽  
Author(s):  
M. J. Argente ◽  
D. M. Abad-Salazar ◽  
E. M. Bermejo-González ◽  
M. L. Garcíaz ◽  
A. López-Palazón
Keyword(s):  
Animal Model ◽  
Immune System ◽  
Health Status ◽  
Blood Cells ◽  
White Blood Cells ◽  
Blood Parameters ◽  
Physiological Status ◽  
Immune Challenge ◽  
Leukocyte Counts ◽  
Over Time

Rabbit is widely used as an experimental animal model in infectious and non-infectious diseases. The haematologic data can be helpful in evaluating the health status of animals over time. The aim of this study was to determine the levels of red blood cells (RBC), white blood cells (WBC) and differential leukocyte counts in 5 nulliparous and 5 multiparous females, i.e. in young and older animals, at mating and at delivery. The values of RBC did not change with age, but WBC and lymphocytes decreased with age, a -33% and a -60% less in multiparous females than nulliparous ones. Monocytes count was double at delivery than at mating. In conclusion, aging on the immune system is manifested as reduction in production of mature lymphocytes and as a result, older females would not respond to immune challenge as robustly as the young ones. Physiological status is only related to production of monocytes.

scholarly journals II. APLASIA OF MARROW AND FATAL INTOXICATION IN DOGS PRODUCED BY ROENTGEN RADIATION OF ALL BONES

1931 ◽  
Vol 53 (3) ◽  
pp. 421-435 ◽  
Author(s):  
Samuel S. Shouse ◽  
Stafford L. Warren ◽  
George H. Whipple
Keyword(s):  
Blood Cells ◽  
White Blood Cells ◽  
Germinal Centers ◽  
Fat Cells ◽  
Red Cell ◽  
Fatal Intoxication ◽  
Blood Smears ◽  
Recent Origin ◽  
Acute Reaction ◽  
Spleen And Lymph Nodes

Constant findings were obtained in the acute reaction to the specified amount of heavily filtered radiation over the bony skeleton. 1. There develops without warning a short and fatal intoxication on the 8th or 9th day after the exposure to the radiation. 2. A profound leucopenia appears after 5 to 6 days and is maintained in the peripheral blood (200 white blood cells or less per c. mm.) for the 2 to 3 days before death. 3. The platelets suddenly disappear from the blood smears the day before death. This has some bearing on the life cycle of the platelet. 4. All of the organs and body structures present extensive and generalized capillary hemorrhage of recent origin. 5. The substance of the spleen and lymph nodes is greatly reduced and the germinal centers are visible only as remnants. 6. The red cell hematocrit reading drops from about 50 per cent or normal to approximately 40 per cent. 7. The bone marrow is depleted of all its cells except the connective tissue and fat cells, blood vessel endothelium, phagocytes filled with brown granules, and occasional normoblasts.

scholarly journals A MATLAB model for diagnosing sickle cells and other blood abnormalities using image processing

2021 ◽  
Vol 11 (6) ◽  
pp. 5060
Author(s):  
Mohammed Al-Momin ◽  
Ammar Almomin
Keyword(s):  
Image Processing ◽  
Blood Cells ◽  
White Blood Cells ◽  
Problem Of Time ◽  
Blood Constituents ◽  
Sickle Cells ◽  
Blood Disorder ◽  
Blood Smears ◽  
High Level ◽  
Processing Techniques

<span lang="EN-US">The conventional method for detecting blood abnormality is time consuming and lacks the high level of accuracy. In this paper a MATLAB based solution has been suggested to tackle the problem of time consumption and accuracy. Three types of blood abnormality have been covered here, namely, anemia which is characterized by low count of red blood cells (RBCs), Leukemia which is depicted by increasing the number of white blood cells (WBCs), and sickle cell blood disorder which is caused by a deformation in the shape of red cells. The algorithm has been tested on different images of blood smears and noticed to give an acceptable level of accuracy. Image processing techniques has been used here to detect the different types of blood constituents. Unlike many other researches, this research includes the blood sickling disorder which is epidemic in certain regions of the world, and offers a more accuracy than other algorithms through the use of detaching overlapped cells strategy.</span>

scholarly journals Hematological changes of Anemia`s in Horses of Equestrian club in Baghdad

2012 ◽  
Vol 36 (0A) ◽  
pp. 92-97
Author(s):  
Faisal G. Habasha
Keyword(s):  
Blood Cell ◽  
Sedimentation Rate ◽  
Red Blood Cell ◽  
Packed Cell Volume ◽  
Blood Cells ◽  
White Blood Cells ◽  
Blood Samples ◽  
Erythrocyte Sedimentation ◽  
Blood Smears ◽  
Hematological Changes

This study was conducted to know thehematological changes of anemia in horsesat equestrian club in Baghdad. Blood samples were collected from 151 horses of both sexes(74 male and 77 female) and different agesrandomly. The study includedred blood cells count, white blood cells, hemoglobin, packed cell volume and differential blood smears, togetherwith erythrocyte sedimentation rate readings. The study showed increased white blood cells count mainly neutrophilwith decreased hemoglobinand red blood cell countin addition to erythrocyte sedimentation rate.The blood smears showeddifferent changes of red blood cell.

scholarly journals Toxicity of 13C-labeled linoleic and linolenic acids for diagnostic breath tests

2019 ◽  
pp. 19-24
Author(s):  
Ya. Ya. Tynio ◽  
G. V. Morozova ◽  
Yu. K. Biryukova ◽  
E. V. Trubnikova ◽  
M. V. Zylkova ◽  
...  
Keyword(s):  
Blood Cells ◽  
White Blood Cells ◽  
Intragastric Administration ◽  
Control Groups ◽  
Subchronic Toxicity ◽  
Breath Tests ◽  
Diagnostic Use ◽  
Tract Function ◽  
Higher Doses

Noninvasive stable isotope breath tests allow highly accurate and safe estimation of liver and biliary tract function. The aim of this study was to test 13С-labeled linoleic and linolenic acids intended for diagnostic use for acute and subchronic toxicity. The acids were synthesized using the patented method. A single intragastric administration of the tested compounds to experimental BALB/c mice and Wistar rats in the amounts exceeding clinical doses 500 to 2500-fold did not cause animal death. In the subchronic toxicity test, the rats received 5 to 25 times higher doses than recommended for clinical use in humans. In a 14-day follow-up period, no significant differences were observed between the main and the control groups in terms of weight, blood count (red blood cells, white blood cells, platelets), and blood biochemistry (hemoglobin, total protein, alkaline phosphatase, alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase, bilirubin). The studied compounds are safe at doses intended for oral administration and are recommended for further preclinical and clinical trials.

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