scholarly journals Effects of Fruits of Lapsi (Nepali Hog Plum) Choerospondias axillaris (roxb.) b.l.Burtt & a.w.Hill on Some Blood Parameters of Major Carp rohu Labeo rohita(H) During Intensive Aquaculture

2020 ◽  
Vol 19 (1) ◽  
pp. 186-195
Author(s):  
Shubha Ratna Shakya ◽  
Shyam Narayan Labh
Keyword(s):  
Blood Cells ◽  
Labeo Rohita ◽  
Control Diet ◽  
White Blood Cells ◽  
Ethanol Extract ◽  
Blood Parameters ◽  
Significant Difference ◽  
Fish Feeds ◽  
Intensive Aquaculture ◽  
And Control

 The complete blood cell count (CBC) is an important and powerful diagnostic tool to monitor the health status of fish in response to changes related to nutrition, water quality, and disease in response to therapy.Thus, the present study was about to know the effect of lapsi fruit pulp (Choerospondias axillaris) on some blood parameters in the fingerlings of rohu Labeo rohita cultured in Corona of Agriculture Hatchery farm, Chitwan, Nepal. Altogether eighteen hapas made of heavy-duty nylon net (1.5m3) were kept and placed inside the pond, distributed linearly, and then 270 fingerlings (@15 fishes/ hapa) were kept distributed randomly. Six practical diets like T1 (0.0 g kg-1), T2 (0.1 g kg-1), T3 (0.2 g kg-1), T4 (0.4 g kg-1), T5 (0.8 g kg-1) and T6 (1.6 g kg-1) were prepared. Feed containing 40% protein was supplemented with the ethanol extract of lapsi fruits. At the end of 90 days of the feeding trial, a significant difference (P< 0.05) in blood parameters were observed between the treated and control diet-fed groups. Hemoglobin (Hb), white blood cells (WBCs), red blood cells (RBCs), packed cell volume (PCV), and other erythrocyte indices were recorded higher in the treated groups. RBC, WBC, Hct, and Hb were found significantly higher in the T4 (0.4 %) diet-fed group. The study showed a minimum of 0.4 % (0.4g kg-1) lapsi fruit extract needed in fish feeds to increase blood parameters to enhance growth and immunity.

scholarly journals Profile of cortisol, glycaemia, and blood parameters of American Bullfrog tadpoles Lithobates catesbeianus exposed to density and hypoxia stressors

2012 ◽  
Vol 32 (suppl 1) ◽  
pp. 91-98 ◽  
Author(s):  
Patricia C. Teixeira ◽  
Danielle C. Dias ◽  
Guilherme C. Rocha ◽  
Antônio M. Antonucci ◽  
Fernanda M. França ◽  
...  
Keyword(s):  
Blood Cells ◽  
White Blood Cells ◽  
Experimental Period ◽  
Blood Parameters ◽  
Air Exposure ◽  
Physiological Profile ◽  
Lithobates Catesbeianus ◽  
American Bullfrog ◽  
Physiological Alterations ◽  
Significant Difference

The aim of this study was to evaluate alterations to the physiological profile (cortisol, glycaemia, and blood parameters) of Lithobates catesbeianus caused by the stressors density and hypoxia. The organisms were in the prometamorphosis stage and exposed to different tadpole densities: 1 tadpole/L (T1), 5 tadpoles/L (T2), and 10 tadpoles/L (T3) for 12 days. The blood was collected through the rupture of the caudal blood vessel and collected under normoxia (immediate collection) and hypoxia (after 15 minutes of air exposure) conditions. Cortisol levels rose on the fourth and eighth days of treatment and returned to basal levels by the end of the experiment. The stressor mechanisms tested did not affect glycaemia. White blood cells (total number of lymphocytes, neutrophils, and eosinophils) showed a significant difference at the twelfth day of the experiment when compared with the start of the experiment. We concluded that, under controlled conditions, a density of up to 10 tadpoles/L and air exposure for 15 minutes did not cause harmful physiological alterations during the experimental period. The answer to these stressors maybe was in another hormonal level (corticosterone).

scholarly journals The effect of domestic effluent on growth and haematological parameters of Clarias gariepinus (Burchell, 1822) (Pisces; Clariidae)

2020 ◽  
pp. 129-136
Author(s):  
Asiata Adekilekun Kolawole ◽  
Reuben Ataitiya ◽  
Quazim Olawale Junaid ◽  
Abdur-Rahman Kolawole Mustapha
Keyword(s):  
Blood Cells ◽  
Clarias Gariepinus ◽  
White Blood Cells ◽  
Blood Parameters ◽  
Behavioral Changes ◽  
Toxicological Effect ◽  
Domestic Effluents ◽  
Set Up ◽  
And Control ◽  
Domestic Effluent

Esta investigación determina el efecto toxicológico de efluentes domésticos en los parámetros sanguíneos de Clarias gariepinus. Se realizaron cuatro tratamientos: Control, 30, 50 y 70 mlL-1. Cada tratamiento fue por triplicado. Se produjeron cambios de comportamiento en los peces expuestos a 50 y 70 mlL-1 del efluente, y no se observó ninguno en los peces Control y los tratados con 30 mlL-1. Hubo diferencias significativas en la ganancia media de peso y longitud entre los peces expuestos al tratamiento y el control. Hubo una reducción significativa (p < 0.05) de los glóbulos rojos. Los glóbulos blancos, el hematocrito, el volumen corpuscular medio y la hemoglobina de los peces expuestos fueron significativamente más altos que en el control (p < 0,05). Los resultados de este estudio sugieren que los efluentes domésticos pueden afectar. This research determines the toxicological effect of domestic effluents on blood parameters of Clarias gariepinus. Four treatment were set up: Control, 30, 50 and 70mlL-1 . Each treatment was done in triplicate. There were behavioral changes in fishes exposed to 50 and 70 mlL-1 of the effluent while none was observed in Control fishes and fishes undergone 30 mlL-1. There were significant differences in the Mean Weight and Mean Length Gain between the fishes exposed to treatment and Control fishes. There was significant reduction in the red blood cells (p < 0.05). White blood cells, hematocrit, mean corpuscular volume and haemoglobin in the fishes exposed were significantly (p < 0.05) higher than Control. Results from this study suggests that domestic effluents can negatively affect the growth and haematology of fishes.

Hematological condition of breeding bulls after a long winter period of operation in the conditions of the Udmurt Republic

2022 ◽  
pp. 67-73
Author(s):  
A. Abilov ◽  
A. Azhmyakov ◽  
I. Novgorodova ◽  
N. Bogolyubova
Keyword(s):  
Red Blood Cells ◽  
Reference Values ◽  
Blood Cells ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Winter Period ◽  
Black And White ◽  
Significant Difference ◽  
White Breed ◽  
Udmurt Republic

Purpose: to study hematological parameters of blood in bulls-producers of dairy breeds on the day of semen collection in the Udmurt Republic after a long winter period of operation, depending on the breeds, age and place of selection.Materials and methods. The work was performed at the Federal Research Center for Animal Husbandry named after Academy Member L. K. Ernst on the basis of AO "Udmurtplem" of the Udmurt Republic in the period from 2020 to 2021 on dairy bulls (n=20) aged 15-69 months, including a purebred Holstein breed of domestic selection (n=6), a Holstein black-and-white breed of European selection (the Netherlands, n=6), a black-and-white breed with blood transfusion on Holsteins on at the level of 94-98% (n=8). The content of white blood cells, red blood cells, hemoglobin and hematocrit, depending on age and breed, was studied on the ABC VET hematological analyzer on the day of taking the seed.Results. It was found that on average, in 20 bulls aged 15-69 months, the level of white blood cells was at the level of reference values of 8.8±0.25 x 109/l, red blood cells 10.3 x 1012/l, which is 50% more than the reference values. The concentration of hemoglobin is 128.0±2.92 g/l, hematocrit is on average 54.3%, with a norm of 24-46%. Breeding bulls at a reliable level, differing in age, showed that some animals had high indicators for red blood cells of 10.3±0.26 x 102, for hematocrit of 54.3% against 24-46% of reference values. Also, according to hematological indicators, there was a tendency to increase the concentration of hemoglobin and hematocrit in European-bred bulls.Conclusion. The study of the variability in hematological parameters depending on the selection showed that there is no significant difference in leukocytes and all indicators are at the level of reference values, and in erythrocytes more than 50% than the highest indicators. The highest hemoglobin values were at the level of 141-156 g/l instead of 128 g/l according to the highest reference values. Hematocrit also showed high max values in all groups in comparison with the reference values of 59-66% versus 46% in the norm. It is necessary when analyzing hematological blood parameters in addition to the average statistical indicators (M+m) also, monitor the variability (min-max) in order to obtain more objective information.

scholarly journals Cytological analysis of the lung material of C57BL/6Y mice in the simulation of acute respiratory distress syndrome

Biomeditsina ◽  
2021 ◽  
Vol 17 (3E) ◽  
pp. 17-22
Author(s):  
O. V. Alimkina ◽  
A. E. Petrenko
Keyword(s):  
Acute Respiratory Distress Syndrome ◽  
Respiratory Distress Syndrome ◽  
Respiratory Distress ◽  
Bronchoalveolar Lavage ◽  
Blood Cells ◽  
Distress Syndrome ◽  
White Blood Cells ◽  
Intact Group ◽  
Significant Difference ◽  
Over Time

The work is devoted to the study of changes in the cellular composition of bronchoalveolar lavage over time in the modeling of acute respiratory distress syndrome (ARDS) in mice. ARDS was modeled by administering α-galactosylceramide and a mixture of lipopolysaccharide with a complete Freud’s adjuvant. After euthanasia, bronchoalveolar lavage was taken for analysis. On this basis, changes in the total number of white blood cells, the percentage of neutrophils and macrophages were assessed. It was found that the percentage of neutrophils in the ARDS group shows a statistically significant difference from that in the intact group, starting from 3 hours after modeling ARDS. Further, a statistically significant decrease in macrophages was observed. 

scholarly journals In-vivo Antiplasmodial Effect of Dihydroartemisinin-Piperaquine-Nitrofurantoin on Plasmodium berghei- Infected Mice

2021 ◽  
pp. 12-20
Author(s):  
Udeme O. Georgewill ◽  
Festus Azibanigha Joseph ◽  
Elias Adikwu
Keyword(s):  
Plasmodium Berghei ◽  
Blood Cells ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Positive Control ◽  
Negative Control ◽  
Antiplasmodial Activity ◽  
Significant Difference ◽  
Tract Infections

Nitrofurantoin (NT) used for the treatment of urinary tract infections may have antiplasmodial activity. Dihydroartemisinin-piperaquine (DP) is an artemisinin based combination therapy used for the treatment of malaria. This study evaluated the antiplasmodial effect of dihydroartemisinin-piperaquine-nitrofurantoin (DP-NT) on mice infected with Plasmodium berghei. Adult Swiss albino mice (30-35 g) of both sexes were used. The mice were randomly grouped, inoculated with Plasmodium berghei, and treated orally with DP (1.7/13.7 mg/kg), NT (57.1 mg/kg) and DP-NT (1.71/13.7/ 57.1 mg/kg), respectively using curative, prophylactic and suppressive tests. The negative control was orally treated with normal saline (0.3 mL), while the positive control was orally treated with chloroquine CQ (10mg/kg). After treatment, blood samples were collected and evaluated for percentage parasitemia, inhibitions and hematological parameters. Liver samples were evaluated for histological changes. The mice were observed for mean survival time (MST). Treatment with DP-NT decreased parasitemia levels when compared to individual doses of DP and NT with significant difference observed at p<0.05. DP-NT prolonged MST when compared to individual doses of DP and NT with significant difference observed at p<0.05. The decrease in packed cell volume, red blood cells, hemoglobin and increase in white blood cells in parasitized mice were significantly restored by DP-NT  when compared to individual doses of DP and NT with difference observed at p<0.05. DP-NT eradicated liver Plasmodium parasite.  NT remarkably increased the antiplasmodial activity of DP. DP-NT may be used for the treatment of malaria.

scholarly journals Evaluation of Raw Yeast Extract (Saccharomyces cerevisiae) as an Ingredient, Additive or Palatability Agent in Wet Diet for Cats

2015 ◽  
Vol 8 (1) ◽  
pp. 1 ◽  
Author(s):  
Lidia M. Lima ◽  
Jose Walter Silva Jr ◽  
Rosana C. S. Ogoshi ◽  
Jessica S. dos Reis ◽  
Janine Franca ◽  
...  
Keyword(s):  
Yeast Extract ◽  
Control Diet ◽  
Latin Squares ◽  
Metabolizable Energy ◽  
Energy Utilization ◽  
Functional Additive ◽  
Apparent Digestibility Coefficient ◽  
Significant Difference ◽  
Adult Cat ◽  
And Control

<p class="1Body">Three experiments were performed to evaluate the effects of strain-specific yeast extract (SSYE) as an ingredient, functional additive or palatability agent when supplemented in its raw form in wet cat food. <em>SSYE as ingredient</em> – SSYE was chemically characterized and its use evaluated through fourteen cats divided into two treatments: control diet (complete wet adult cat food) and control diet with 30 % replacement by SSYE. The results of apparent digestibility coefficient of SSYE were 71.64 % for dry matter, 72.55 % for organic matter, 50.78 % for ashes, 78.59 % for crude protein, 84.33 % for the energy gross and digestible and metabolizable energy value, respectively, of 4,247 and 4,163 kcal/kg, these results indicated that SSYE is comparable to other protein sources for cat’s food. <em>SSYE as a functional additive</em> - twelve cats were distributed into two 6x6 latin squares (treatments; experimental periods), and the treatments were control diet and replacement levels ranged from 2 % to 10 % SSYE. The following parameters were evaluated: digestibility, energy utilization, nitrogen balance, serum urea and creatinine levels. No differences were found. <em>SSYE as palatability agent</em> – Were used twenty cats by comparing the control diet with 2 % replacement by SSYE. A significant difference (P &lt; 0.01) was observed with a preference for control diet. SSYE is a potential protein source for cats; however, it is not effective as additive and may compromise palatability when supplemented in its raw form in complete wet cat food.</p>

scholarly journals Critical requirement for Stat5 in a mouse model of polycythemia vera

Blood ◽  
2012 ◽  
Vol 119 (15) ◽  
pp. 3539-3549 ◽  
Author(s):  
Dongqing Yan ◽  
Robert E. Hutchison ◽  
Golam Mohi
Keyword(s):  
Polycythemia Vera ◽  
Blood Cells ◽  
Myeloproliferative Neoplasms ◽  
White Blood Cells ◽  
Strong Support ◽  
Primary Myelofibrosis ◽  
Blood Parameters ◽  
Critical Function ◽  
Critical Requirement ◽  
Multiple Signaling

The JAK2V617F mutation has been identified in most cases of Ph-negative myeloproliferative neoplasms (MPNs) including polycythemia vera (PV), essential thrombocythemia (ET), and primary myelofibrosis (PMF). Expression of JAK2V617F results in constitutive activation of multiple signaling molecules/pathways. However, the key signaling downstream of JAK2V617F required for transformation and induction of MPNs remains elusive. Using a mouse genetic strategy, we show here that Stat5 is absolutely required for the pathogenesis of PV induced by Jak2V617F. Whereas expression of Jak2V617F in mice resulted in all the features of human PV, including an increase in red blood cells, hemoglobin, hematocrit, white blood cells, platelets, and splenomegaly, deletion of Stat5 in the Jak2V617F knockin mice normalized all the blood parameters and the spleen size. Furthermore, deletion of Stat5 completely abrogated erythropoietin (Epo)–independent erythroid colony formation evoked by Jak2V617F, a hallmark feature of PV. Re-expression of Stat5 in Stat5-deficient Jak2V617F knockin mice completely rescued the defects in transformation of hematopoietic progenitors and the PV phenotype. Together, these results indicate a critical function for Stat5 in the pathogenesis of PV. These findings also provide strong support for the development of Stat5 inhibitors as targeted therapies for the treatment of PV and other JAK2V617F-positive MPNs.

scholarly journals Experimental infections of baboons (Papio spp.) and vervet monkeys (Cercopithecus aethiops) with Trichinella zimbabwensis and successful treatment with ivermectin

2008 ◽  
Vol 75 (2) ◽  
Author(s):  
S. Mukaratirwa ◽  
B. M. Dzoma ◽  
E. Matenga ◽  
S. D. Ruziwa ◽  
L. Sacchi ◽  
...  
Keyword(s):  
Packed Cell Volume ◽  
Post Infection ◽  
Blood Cells ◽  
White Blood Cells ◽  
Clinical Signs ◽  
Blood Parameters ◽  
Cercopithecus Aethiops ◽  
Vervet Monkeys ◽  
Muscular Pain ◽  
Normal Ranges

Experimental Trichinella zimbabwensis infections were established in three baboons (Papios p.)and four vervet monkeys (Cercopithecuase thiops) and the clinical-pathological manifestations assessed. The infected animals showed clinical signs ranging from fever, diarrhoea, periorbitaol edema and muscular pain in varying degrees. One baboon became blind due to the infection. Levels of creatinine phosphokinase and lactated ehydrogenase increased to reach a peak on Day 42 post-infection(pi)for both baboons and monkeys. Blood parameters such as packed cell volume, levels of red blood cells and white blood cells did not change significantly from the normal ranges except for the levels of eosinophils which peaked above the normal ranges at Day 28 and 56 pi in baboons and at Day 56 pi in monkeys.

Biochemical and haematological changes associated with short periods of work in draught oxen

1989 ◽  
Vol 48 (2) ◽  
pp. 375-384 ◽  
Author(s):  
R. Anne Pearson ◽  
R. F. Archibald
Keyword(s):  
Heart Rate ◽  
Blood Cells ◽  
Inorganic Phosphate ◽  
White Blood Cells ◽  
Blood Parameters ◽  
Blood Levels ◽  
Blood Samples ◽  
Haematological Changes ◽  
Work Done ◽  
Friesian Cattle

ABSTRACTBlood samples were taken from three Brahman × Friesian cattle while they walked for 1 h daily on a treadmill pulling 20 or 25 kg weights suspended in a cage. Heart rate and energy expenditure during work were closely correlated. The work had no significant effect on blood levels of red cells, haemoglobin, packed cell volume, total protein, albumin, glycerol, urea, Mg, Ca, Na, K and chloride. White blood cells, glucose, lactate, free fatty acids, P-hydroxybutyrate and inorganic phosphate were affected by work although the changes were shortlived and values had returned to resting levels 75 min after work finished. The changes were similar in each animal and indicated work done by draught cattle is largely at a submaximal level. Apart from lactate no blood parameters were identified that could be usefully used to compare performance.

Reference Values for Selected Blood Parameters in Rabbits: Effects of Age and Physiological Status

2019 ◽  
Author(s):  
M. J. Argente ◽  
D. M. Abad-Salazar ◽  
E. M. Bermejo-González ◽  
M. L. Garcíaz ◽  
A. López-Palazón
Keyword(s):  
Animal Model ◽  
Immune System ◽  
Health Status ◽  
Blood Cells ◽  
White Blood Cells ◽  
Blood Parameters ◽  
Physiological Status ◽  
Immune Challenge ◽  
Leukocyte Counts ◽  
Over Time

Rabbit is widely used as an experimental animal model in infectious and non-infectious diseases. The haematologic data can be helpful in evaluating the health status of animals over time. The aim of this study was to determine the levels of red blood cells (RBC), white blood cells (WBC) and differential leukocyte counts in 5 nulliparous and 5 multiparous females, i.e. in young and older animals, at mating and at delivery. The values of RBC did not change with age, but WBC and lymphocytes decreased with age, a -33% and a -60% less in multiparous females than nulliparous ones. Monocytes count was double at delivery than at mating. In conclusion, aging on the immune system is manifested as reduction in production of mature lymphocytes and as a result, older females would not respond to immune challenge as robustly as the young ones. Physiological status is only related to production of monocytes.

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