scholarly journals Green Fabrication of Silver Nanoparticles by Gum Tragacanth (Astragalus gummifer): A Dual Functional Reductant and Stabilizer

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Aruna Jyothi Kora ◽  
Jayaraman Arunachalam
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Water Soluble ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
X Ray Diffraction ◽  
Gum Tragacanth ◽  
Synthesis Of Nanoparticles ◽  
Dual Functional ◽  
Transmission Electron

A simple and ecofriendly procedure have been devised for the green synthesis of silver nanoparticles using the aqueous extract of gum tragacanth (Astragalus gummifer), a renewable, nontoxic natural phyto-exudate. The water soluble components in the gum act as reductants and stabilizers. The generated nanoparticles were analyzed using UV-visible spectroscopy, transmission electron microscopy, X-ray diffraction, Fourier transform-infrared spectroscopy, and Raman spectroscopy. The role of gum concentration and reaction time on the synthesis of nanoparticles was studied. By regulating the reaction conditions, spherical nanoparticles of13.1±1.0 nm size were produced. Also, the possible functional groups involved in reduction and capping of nanoparticles has been elucidated. The antibacterial activity of the fabricated nanoparticles was tested on model Gram-negative and Gram-positive bacterial strains with well-diffusion method. These nanoparticles exhibited considerable antibacterial activity on both the Gram classes of bacteria, implying their potential biomedical applications.

Synthesis and Antibacterial Activity of Silver Nanoparticles Against Escherichia coli and Pseudomonas sp.

2021 ◽  
Author(s):  
Md. Irfanul Hoque ◽  
Sultana Afrin Jahan Rima ◽  
Md. Salah Uddin ◽  
M. Julkarnain
Keyword(s):  
Escherichia Coli ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Chemical Reduction ◽  
Bacterial Species ◽  
Sodium Dodecyl ◽  
Antibacterial Properties ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
X Ray Diffraction

Silver nanoparticles (AgNPs) have been synthesized by chemical reduction method using ascorbic acid as reducing agent. Silver nitrate (AgNO[Formula: see text] and sodium dodecyl sulfate (SDS) have been used as precursor and stabilizer, respectively. The prepared samples were characterized by UV-visible spectroscopy, scanning electron microscopy (SEM) and X-ray diffraction (XRD). The antibacterial activity of prepared silver nanoparticles has been assessed by using the disc diffusion method against pathogenic, gram-negative bacterial strains including Escherichia coli and Pseudomonassp. To evaluate the potential antibacterial properties of AgNPs, the discs have been impregnated and dried with three different doses like 50, 100 and 150[Formula: see text][Formula: see text]l of 20[Formula: see text][Formula: see text]g/ml concentrated AgNPs solution and placed on the Petri-dishes. The antibiotic kanamycin (5[Formula: see text][Formula: see text]g) was used as control. In all the cases, a clear and distinct zone of inhibition is observed, which suggests that AgNPs can be used as effective growth inhibitors of various bacterial species and would be promising candidate for future development of antibacterial agents.

scholarly journals Biophysical and Characterizations of Silver Nanoparticles used as Salmonella typhi Detector

2014 ◽  
Vol 2 (4) ◽  
pp. 510-515
Author(s):  
Hala Moustafa Ahmed
Keyword(s):  
Silver Nanoparticles ◽  
Salmonella Typhi ◽  
Bacterial Strains ◽  
X Ray Diffraction ◽  
X Ray ◽  
Transmission Electron ◽  
Vis Spectroscopy ◽  
Combined Action ◽  
Hog Plum ◽  
Transmission Electronmicroscopy

The present study mainly focuses of combined action of Nepali hog plum as well as citrate synthesized silver nanoparticles (AgNPs) and Amikacin, as an antibiotic. The synergistic actions of citrate stabilized silver nanoparticles (AgNPs with chem) were compared with that of Nepali hog plum Choerospondia saxillaris (Lapsi) synthesized silver nanoparticles (AgNPs with plant), together with action of antibiotic onselected bacterial strains of Salmonella typhi. The synthesized AgNPs were characterized through UV-Vis spectroscopy, Transmission electronmicroscopy and X-ray diffraction technique. The size of the synthesized silver nanoparticles was measured by Transmission Electron Microscope (TEM) and X-ray diffraction (XRD).DOI: http://dx.doi.org/10.3126/ijasbt.v2i4.11127 Int J Appl Sci Biotechnol, Vol. 2(4): 510-515 

scholarly journals Anticoagulant, thrombolytic and antibacterial activities of Euphorbia acruensis latex-mediated bioengineered silver nanoparticles

2019 ◽  
Vol 8 (1) ◽  
pp. 590-599 ◽  
Author(s):  
Kaushik Roy ◽  
Ambikesh K. Srivastwa ◽  
Chandan K. Ghosh
Keyword(s):  
Silver Nanoparticles ◽  
Analytical Techniques ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
X Ray Diffraction ◽  
Antibacterial Study ◽  
X Ray ◽  
Transmission Electron ◽  
Human Blood Samples ◽  
Green Synthesized Silver Nanoparticles

Abstract In this report, we present a simple and unexplored procedure for green synthesis of silver nanoparticles featuring exudation of Euphorbia acruensis along with the study of its antibacterial and anticoagulant properties. Analytical techniques like ultraviolet visible spectroscopy (UV-Vis), X-ray diffraction (XRD) and high resolution transmission electron microscopy (HRTEM) were used to analyse the production, crystallinity and morphology of bio-reduced silver nanoparticles. The antibacterial study was performed by following standard disc diffusion method. Most importantly, the anticoagulant and thrombolytic activities of biogenic silver nanoparticles were evaluated by addition of nanoparticles to human blood samples under practical conditions. These green synthesized silver nanoparticles were found to have potent antibacterial, anticoagulant and thrombolytic properties which make them an attractive choice for future medical applications.

scholarly journals Fortunella japonica extract as a reducing agent for green synthesis of silver nanoparticles

2018 ◽  
Vol 7 (3) ◽  
pp. 1570
Author(s):  
Nguyen Phung Anh ◽  
Truong Thi Ai Mi ◽  
Duong Huynh Thanh Linh ◽  
Nguyen Thi Thuy Van ◽  
Hoang Tien Cuong ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Volume Ratio ◽  
Diffusion Method ◽  
High Antibacterial Activity ◽  
Agno3 Solution ◽  
Transmission Electron ◽  
Vis Spectroscopy ◽  
Average Size

A rapid way of synthesizing silver nanoparticles (AgNPs) by treating Ag+ ions with a green Fortunella Japonica (F.J.) extract as a combined reducing and stabilizing agent was investigated. The reaction solutions were monitored using UV-Vis spectroscopy, the size and shape of crystals were determined by scanning electron microscopy and transmission electron microscopy, the crystalline phases of AgNPs were presented by X–ray diffraction, and the relation of nanoparticles with Fortunella Japonica extract was confirmed using fourier transform infrared spectroscopy. The results indicated that no formation of AgNPs had taken place in the dark during 24 hours at room temperature and 40 oC. Meanwhile, it was found that the rate of AgNPs formation increased rapidly under the sunlight. The effects of the synthesis factors on the AgNPs formation were investigated. The suitable conditions for the synthesis of AgNPs using F.J. extract were determined as follows: F.J. extract was mixed with AgNO3 1.75 mM solution with the volume ratio of 3.5 AgNO3 solution/1.5 F.J. Extract, stirred 300 rpm for 150 minutes at 40 oC under sunlight illumination. At these conditions, AgNPs showed high crystalline structure with the average size of 15.9 nm. The antibacterial activity of silver nanoparticles was determined by agar well diffusion method against E. coli and B. subtilis bacteria. The green synthesized AgNPs performed high antibacterial activity against both bacteria.  

ANTIFUNGAL STUDIES ON BIOCOMPATIBLE POLYMER ENCAPSULATED SILVER NANOPARTICLES

2011 ◽  
Vol 10 (04n05) ◽  
pp. 1179-1183
Author(s):  
R. ANITHA ◽  
B. KARTHIKEYAN ◽  
T. PANDIYARAJAN ◽  
S. VIGNESH ◽  
R. ARTHUR JAMES ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antifungal Activity ◽  
Antimicrobial Properties ◽  
Water Soluble ◽  
Diffusion Method ◽  
Electron Microscopic ◽  
Disk Diffusion Method ◽  
Water Soluble Polymer ◽  
Transmission Electron ◽  
Standard Disk

Silver nanoparticles are known to have inhibitory antimicrobial properties. In this letter, we report the synthesis of silver nanoparticles by using biocompatible, water soluble polymer through polyol method. Optical absorption spectrum of the prepared particles shows an absorption peak around 433 nm which is because of Surface Plasmon Resonance (SPR) of silver nanoparticles. Fourier transform infrared (FTIR) studies were done to identify the interaction of the nanoparticle and polymer. Transmission Electron Microscopic (TEM) studies confirm that the prepared particles are ~ 100 nm in size. Antifungal activity was studied through standard disk diffusion method. Studies show the prepared particles are potential candidates for the antifungal activity.

scholarly journals THERAPEUTIC ROLE OF AZADIRACHTA INDICA LEAF EXTRACT AGAINST UTI (URINARY TRACT INFECTION)

2020 ◽  
pp. 5-7
Author(s):  
K. Dinesh Singh
Keyword(s):  
Silver Nanoparticles ◽  
Urinary Tract Infection ◽  
Antibacterial Activity ◽  
Urinary Tract ◽  
Tract Infection ◽  
Azadirachta Indica ◽  
Diffusion Method ◽  
X Ray Diffraction ◽  
Micro Organisms

Azadirachta indica is a multipurpose tree with multiple health benefits. Different parts of the plant are shown to exhibit antimicrobial effects against a wide variety of micro organisms. In the present study we investigate the antibacterial activity of Azadirachta indica extract against UTI (urinary tract infection) causing bacteria. Silver nanoparticles could be synthesized using the leaves of Azadirachta indica as reducing agent. The resulting silver nanoparticles were characterized using UV-Vis spectrophotometer, X-ray Diffraction (XRD), Scanning Electron Microscope (SEM), and Fourier Transformations Infra Red (FTIR) spectroscopic analysis. The antibacterial activity was assayed by Agar well diffusion method using 20µl each of sterile Nutrient Agar (NA) (Hi-Media) and Potato-Dextrose Agar (PDA). The resulting silver nanoparticles exhibited best inhibitory activities against Escherichia coli and Proteus mirabilis which are main causing agents for UTI.

Preparation and Antibacterial Activity of Silver Ions-Substituted Hydroxyapatite/Titania

2006 ◽  
Vol 510-511 ◽  
pp. 78-81 ◽  
Author(s):  
Guo Yu Lv ◽  
Yu Bao Li ◽  
Ai Ping Yang ◽  
Xiang Zhang ◽  
Wei Hu Yang ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Composite Materials ◽  
Silver Ions ◽  
Antibacterial Activities ◽  
Bacterial Strains ◽  
X Ray Diffraction ◽  
Disk Diffusion Test ◽  
Transmission Electron ◽  
Dose Dependent ◽  
Better Than

In this paper a series of silver ions-substituted hydroxyapatites (HA) were prepared. The antibacterial activities of these materials on textiles against bacteria have been investigated. Titania (TiO2) was selectively added into the materials to decrease the silver-ions concentration to get the same active antimicrobial effects. The microstructure, the shape and size, concentration of silver, and the groups of the composite materials were characterized using transmission electron microscopy (TEM), infrared spectroscopy (IR), Atomic absorption spectroscopy (AAS), and X-ray diffraction (XRD). The results showed that there was a dose dependent-effect of silver-ions concentration from the disk diffusion test. The higher the silver-ions concentration, the better the antibacterial activity of the composite materials was. Keeping silver-ions concentration constant, the antibacterial activity of the materials for adding Titania was better than that of without Titania. Moreover, the addition of Titania would inhibit the discolouration of the composite materials. The antibacterial activities of the composite materials differentiate to some extent with the bacterial strains.

Biogenic of silver nanoparticles using ayurveda tooth powder and its antibacterial activity of extended-spectrum beta-lactamases producing E.coli

2018 ◽  
Vol 2 (1) ◽  
Author(s):  
Abdullah A. Alarfajj ◽  
Mohammedsaleh Almallahi ◽  
Murugan A. Munusamy ◽  
Mickymaray Suresh ◽  
Wael Alturaiki
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Low Cost ◽  
Diffusion Method ◽  
Tem Analysis ◽  
Beta Lactamases ◽  
Extended Spectrum ◽  
Transmission Electron ◽  
Vis Spectroscopy ◽  
Extended Spectrum Beta Lactamases

Extended-spectrum beta-lactamases (ESBL) are enzymes produced by E. coli like some gram negative bacteria. The patients who are affected by ESBL producing bacteria facing a major problem and they may need different β- lactam antibiotics to treat the infection. But this extensive use of β- lactam antibiotics against ESPLs creating major public health threat.  As an alternative currently many eco- friendly, non-toxic, low cost nanoparticles are synthesizing by biogenic way used as an alternative for the β- lactam antibiotics. In the present study silver nanoparticles (AgNPs) were synthesized using Ayurveda Toothpowder. The synthesized AgNPs were characterized using ultraviolet (UV)-visible (vis) spectroscopy, scanning electron microscopy (SEM), and transmission electron microscope (TEM) analysis and finally the antibacterial activity was performed against ESBL producing bacteria by well diffusion method. Antibacterial tests against ESPL producing E.coli cells using biogenic synthesized AgNPs showed significant antibacterial effect at low concentration of AgNPs. The results proved that the biogenic synthesised nanoparticles using Toothpowder extract would help to arrest ESBL producing bacteria a

scholarly journals GREEN SYNTHESIS, CHARACTERIZATION OF SILVER NANOPARTICLES OF A MARINE RED ALGA SPYRIDIA FUSIFORMIS AND THEIR ANTIBACTERIAL ACTIVITY

2017 ◽  
Vol 9 (5) ◽  
pp. 192 ◽  
Author(s):  
Subbiah Murugesan ◽  
Sundaresan Bhuvaneswari ◽  
Vajiravelu Sivamurugan
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Green Synthesis ◽  
Red Alga ◽  
Diffusion Method ◽  
Present System ◽  
X Ray Diffraction ◽  
Visible Spectroscopy ◽  
X Ray ◽  
Uv Visible

Objective: In the present system, the green synthesis of silver nanoparticles using marine the red alga Spyridia fusiformis and antibacterial activity was carried out.Methods: The seaweed extract was used for the synthesis of AgNPs at room temperature. The silver nanoparticles were characterized by using UV–Visible spectroscopy, Fourier transform infrared spectroscopy, transmission electron microscope and X-ray diffraction (XRD) techniques. The antibacterial activity of biosynthesized silver nanoparticles was carried out by disc diffusion method against pathogenic bacteria.Results: The UV-visible spectroscopy revealed surface plasmon resonance at 450 nm. The FT-IR measurements showed the possible functional groups responsible for the formation of nanoparticles. The X-ray diffraction analysis showed that the particles were crystalline in nature. TEM micrograph has shown the formation of silver nanoparticles with the size in the range of 5–50 nm. The silver nanoparticles synthesized from the S. fusiformis showed higher activity and proved their efficacy in controlling the pathogenic bacterial strains. The nanoparticles showed highest inhibition activity on K. pneumaniae and S. aureus up to 26 and 24±0.01 mm at 100 μg/ml of nanoparticles.Conclusion: The synthesised AgNPs have shown the best antibacterial activity against human pathogens E. coli, K. pneumoniae, S. aureus and P. aeruginosa. The above eco-friendly AgNPs synthesis procedure could be a viable solution for industrial applications in the future and therapeutic needs.

scholarly journals Ecofriendly synthesis of silver nanoparticles from leaves extract of Phyllanthus niruri (L.) and their antibacterial properties

2019 ◽  
Vol 9 (1-s) ◽  
pp. 196-200
Author(s):  
G Amalorpavamary ◽  
G Dineshkumar ◽  
K Jayaseelan
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Antibacterial Properties ◽  
Good Alternative ◽  
Diffusion Method ◽  
Surface Plasmon Resonance Peak ◽  
Phyllanthus Niruri ◽  
Plasmon Resonance Peak ◽  
Synthesis Of Nanoparticles ◽  
Average Size

In recent times, plant-mediated synthesis of nanoparticles has garnered wide interest owing to its inherent features such as rapidity, simplicity, eco-friendliness and cheaper costs. For the first time, silver nanoparticles were successfully synthesized using Phyllanthus niruri leaf extract in the current investigation. The silver nanoparticles were characterized by UV–Vis spectrophotometer and the characteristic surface plasmon resonance peak was identified to be 423 nm. The morphology of the silver nanoparticles was characterized by scanning electron microscopy (SEM). The size of the silver nanoparticles was found to be 10-50 nm, with an average size 15 nm.  FTIR analysis was done to identify the functional groups responsible for the synthesis of the AgNPs. The antibacterial potential of synthesized AgNPs was compared with that of aqueous extracts of P.niruri by well diffusion method. The AgNPs at 50µl concentration significantly inhibited bacterial growth against A.hydrophila (16 ± 0.09 mm). Thus AgNPs showed broad spectrum antibacterial activity at lower concentration and may be a good alternative therapeutic approach in future. Keywords: Phyllanthus niruri, AgNps, Aeromonas hydrophila, Antibacterial Activity.

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