Biogenic of silver nanoparticles using ayurveda tooth powder and its antibacterial activity of extended-spectrum beta-lactamases producing E.coli
Extended-spectrum beta-lactamases (ESBL) are enzymes produced by E. coli like some gram negative bacteria. The patients who are affected by ESBL producing bacteria facing a major problem and they may need different β- lactam antibiotics to treat the infection. But this extensive use of β- lactam antibiotics against ESPLs creating major public health threat. As an alternative currently many eco- friendly, non-toxic, low cost nanoparticles are synthesizing by biogenic way used as an alternative for the β- lactam antibiotics. In the present study silver nanoparticles (AgNPs) were synthesized using Ayurveda Toothpowder. The synthesized AgNPs were characterized using ultraviolet (UV)-visible (vis) spectroscopy, scanning electron microscopy (SEM), and transmission electron microscope (TEM) analysis and finally the antibacterial activity was performed against ESBL producing bacteria by well diffusion method. Antibacterial tests against ESPL producing E.coli cells using biogenic synthesized AgNPs showed significant antibacterial effect at low concentration of AgNPs. The results proved that the biogenic synthesised nanoparticles using Toothpowder extract would help to arrest ESBL producing bacteria a