scholarly journals Ecofriendly synthesis of silver nanoparticles from leaves extract of Phyllanthus niruri (L.) and their antibacterial properties

2019 ◽  
Vol 9 (1-s) ◽  
pp. 196-200
Author(s):  
G Amalorpavamary ◽  
G Dineshkumar ◽  
K Jayaseelan
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Antibacterial Properties ◽  
Good Alternative ◽  
Diffusion Method ◽  
Surface Plasmon Resonance Peak ◽  
Phyllanthus Niruri ◽  
Plasmon Resonance Peak ◽  
Synthesis Of Nanoparticles ◽  
Average Size

In recent times, plant-mediated synthesis of nanoparticles has garnered wide interest owing to its inherent features such as rapidity, simplicity, eco-friendliness and cheaper costs. For the first time, silver nanoparticles were successfully synthesized using Phyllanthus niruri leaf extract in the current investigation. The silver nanoparticles were characterized by UV–Vis spectrophotometer and the characteristic surface plasmon resonance peak was identified to be 423 nm. The morphology of the silver nanoparticles was characterized by scanning electron microscopy (SEM). The size of the silver nanoparticles was found to be 10-50 nm, with an average size 15 nm.  FTIR analysis was done to identify the functional groups responsible for the synthesis of the AgNPs. The antibacterial potential of synthesized AgNPs was compared with that of aqueous extracts of P.niruri by well diffusion method. The AgNPs at 50µl concentration significantly inhibited bacterial growth against A.hydrophila (16 ± 0.09 mm). Thus AgNPs showed broad spectrum antibacterial activity at lower concentration and may be a good alternative therapeutic approach in future. Keywords: Phyllanthus niruri, AgNps, Aeromonas hydrophila, Antibacterial Activity.

scholarly journals Studies on Biosynthesis of Silver Nanoparticles Using Rhizopus sp. and its Antibacterial Efficacy on E. coli MDR Strains

2014 ◽  
Vol 23 ◽  
pp. 27-35
Author(s):  
Jyothi Hiremath ◽  
Vandana Rathod ◽  
Shivaraj Ninganagouda ◽  
Dattu Singh ◽  
K. Prema
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Human Life ◽  
Antibacterial Properties ◽  
Pharmaceutical Products ◽  
Diffusion Method ◽  
Plasmon Resonance Peak ◽  
Synthesis Of Nanoparticles ◽  
E Coli ◽  
Vis Spectroscopy

Nanotechnology is a field that is burgeoning day by day, making an impact in all spheres of human life. Biological methods of synthesis have paved way for the “greener synthesis” of nanoparticles and these have proven to be better methods due to slower kinetics, they offer better manipulation and control over crystal growth and their stabilization. In this context we have investigated extracellular biosynthesis of silver nanoparticles (AgNPs) using cell-free extract of Rhizopus spp.. Formation of AgNPs was indicated by the change in the colour of the cellfree extract from yellow to dark brown under static condition after 48 hrs of incubation. Characterization of AgNPs was carried out by UV-Vis Spectroscopy which gave sharp plasmon resonance peak at 429 nm corresponding to spherical shaped nanoparticles. Transmission electron microscopy (TEM) micrograph showed formation of well-dispersed AgNPs in the range of 25-50 nm. Scanning electron microscopy (SEM) showed the particles to be uniformly dispersed without agglomeration with smooth morphology. EDS showed the presence of elemental silver at 3kev. X-ray diffraction (XRD)-spectrum of the AgNPs exhibited 2θ¸ values corresponding to nanocrystal. These biosynthesized AgNPs were used to study their antimicrobial activity against Multi-drug resistant (MDR) E. coli strains, by Agar diffusion method. Zone of inhibition was measured. Synthesis of nanosized particles with antibacterial properties, which are called "nanoantibiotics", is of great interest in the development of new pharmaceutical products.

A Novel Green Biogenic Synthesis of Silver Nanoparticles using Tabebuia rosea (Bertol.) DC Fruit Extract and Its Antioxidant and Antibacterial Potential

2021 ◽  
Vol 14 (1) ◽  
pp. 5323-5333
Author(s):  
Dr. Guru Kumar Dugganaboyana ◽  
Ramya Jayendra ◽  
Arpitha Narayan ◽  
Meghana Siddappa Konasur
Keyword(s):  
Silver Nanoparticles ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Fruit Extract ◽  
Synthesis Of Nanoparticles ◽  
Drug Molecules ◽  
Agar Disc ◽  
Tabebuia Rosea ◽  
Average Size ◽  
Antibacterial Potential

Plant based synthesis of nanoparticles has generated worldwide interest because of cost-effectiveness, eco-friendly nature and abundance of applications. In the present investigation , antimicrobial potential of silver nanoparticles (AgNPs) of aqueous extract of Tabebuia rosea (Bertol.) DC (T. rosea) fruit extract has been investigated. Agar disc diffusion method was used for determining the antimicrobial activity of selected aqueous fruit extract AgNPs. Phytochemical analysis of aqueous fruit extract of T. rosea fruit revealed the presence of alkaloids, flavonoids, tannins, phenols, carbohydrates, glycosides, Vitamin-C, proteins and terpenoids. AgNPs synthesis using T. rosea aqueous fruit extract and characterized by UV-Visible spectroscopy showed a peak at 420 nm and average size of 82.9 nm, FT-IR analysis, dynamic light scattering, scanning electron microscope, EDX and X-ray diffraction analysis. Evaluation of antibacterial activity of green synthesized AgNPs recorded the more potent activity against selected human bacterial pathogens. The results obtained indicated that the fruit extract of T. rosea as well as AgNPs have strong and effective antibacterial potential that provide marvelous source for the development of new drug molecules of herbal origin which may be used for the welfare of humanity.

scholarly journals Green Synthesis and Antibacterial Activity of HAp@Ag Nanocomposite Using Centella asiatica (L.) Urban Extract and Eggshell

2020 ◽  
Vol 2020 ◽  
pp. 1-12
Author(s):  
Xuan Nui Pham ◽  
Hoa Thi Nguyen ◽  
Ngan Thi Pham
Keyword(s):  
Electron Microscopy ◽  
Antibacterial Activity ◽  
Green Synthesis ◽  
Antibacterial Properties ◽  
Centella Asiatica ◽  
Diffusion Method ◽  
Ag Nps ◽  
X Ray ◽  
Synthesis Of Nanoparticles ◽  
Green Approach

In recent years, the green synthesis of nanoparticles via biological processes has attracted considerable attention. Herein, we introduce a facile and green approach for the synthesis of poriferous silver nanoparticles (Ag-NPs) decorated hydroxylapatite (HAp@Ag) nanoparticles with excellent antibacterial properties. All the nanocomposites were fully characterized in the solid state via various techniques such as X-ray powder diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), and energy-dispersive X-ray spectrometer (EDX), in which the synthesized Ag-NPs (24 nm in diameter) and their homogeneous incorporation on HAp have been studied by ultraviolet-visible (UV-vis) technique, transmission electron microscopy (TEM), and dynamic light scattering (DLS) analysis. The obtained results indicate that the structure and morphology of HAp have no significant changes after the incorporation of Ag-NPs on its surface. Moreover, an impressive antibacterial activity of HAp@Ag nanocomposite against Gram-positive bacterium Staphylococcus aureus and Gram-negative bacteria Escherichia coli and Pseudomonas aeruginosa has been recorded by using the agar well diffusion method. As a result, the HAp@Ag nanocomposite promises to be a great biomedical material with high antibacterial properties.

Phytochemical Synthesis of Silver Nanoparticles Using Anthemis Nobilis Extract and Its Antibacterial Activity

2020 ◽  
Vol 234 (3) ◽  
pp. 531-540
Author(s):  
Saba Ghamipoor ◽  
Faeze Fayyazi ◽  
Saeed Bahadorikhalili
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Green Synthesis ◽  
Nitrate Solution ◽  
Synthesis Method ◽  
Silver Ions ◽  
Good Alternative ◽  
Ft Ir ◽  
Average Size ◽  
Physical And Chemical

AbstractIn this work, green synthesis of silver nanoparticles is described by phytochemical reducing silver nitrate aqueous solution using Anthemis nobilis. For this purpose, Anthemis nobilis extract was used for the synthesis of silver nanoparticles as both surfactant and reducing agent. Green synthesis method is a good alternative to physical and chemical methods, since it is fast, simple, environmentally-friendly and economic. The produced nanoparticles are identified using FE-SEM, EDX, and FT-IR and Uv/Vis techniques. Formation of silver nanoparticles is verified in 430–420 nm range. Reduction of silver ions by hydroxyl functional group is also confirmed by FT-IR device. EDX device confirms the presence of a peak for Ag element without any impurity peak. Silver nanoparticles are identified by FE-SEM device and found to have average size between 17 and 42 nm. Also, the antibacterial activity of the synthesized nanoparticles is compared with that of staphyloccusaureus and pseudomonasa aeruginosa and the maximum inhibitory activity against the bacteria is obtained using 1 mM nitrate solution.

scholarly journals Fortunella japonica extract as a reducing agent for green synthesis of silver nanoparticles

2018 ◽  
Vol 7 (3) ◽  
pp. 1570
Author(s):  
Nguyen Phung Anh ◽  
Truong Thi Ai Mi ◽  
Duong Huynh Thanh Linh ◽  
Nguyen Thi Thuy Van ◽  
Hoang Tien Cuong ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Volume Ratio ◽  
Diffusion Method ◽  
High Antibacterial Activity ◽  
Agno3 Solution ◽  
Transmission Electron ◽  
Vis Spectroscopy ◽  
Average Size

A rapid way of synthesizing silver nanoparticles (AgNPs) by treating Ag+ ions with a green Fortunella Japonica (F.J.) extract as a combined reducing and stabilizing agent was investigated. The reaction solutions were monitored using UV-Vis spectroscopy, the size and shape of crystals were determined by scanning electron microscopy and transmission electron microscopy, the crystalline phases of AgNPs were presented by X–ray diffraction, and the relation of nanoparticles with Fortunella Japonica extract was confirmed using fourier transform infrared spectroscopy. The results indicated that no formation of AgNPs had taken place in the dark during 24 hours at room temperature and 40 oC. Meanwhile, it was found that the rate of AgNPs formation increased rapidly under the sunlight. The effects of the synthesis factors on the AgNPs formation were investigated. The suitable conditions for the synthesis of AgNPs using F.J. extract were determined as follows: F.J. extract was mixed with AgNO3 1.75 mM solution with the volume ratio of 3.5 AgNO3 solution/1.5 F.J. Extract, stirred 300 rpm for 150 minutes at 40 oC under sunlight illumination. At these conditions, AgNPs showed high crystalline structure with the average size of 15.9 nm. The antibacterial activity of silver nanoparticles was determined by agar well diffusion method against E. coli and B. subtilis bacteria. The green synthesized AgNPs performed high antibacterial activity against both bacteria.  

scholarly journals Multifunction Finishing of Cellulose Based Fabrics via 3-Chloro-2-Hydroxypropyl Trimethyl Ammonium Chloride (Quat-188) and Silver Nanoparticles (AgNPs) to Improve its Dyeability and Antibacterial

2020 ◽  
Vol 11 (4) ◽  
pp. 11666-11678
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Antibacterial Properties ◽  
Cotton Fabrics ◽  
Diffusion Method ◽  
Acid Dyes ◽  
Color Strength ◽  
Disk Diffusion Method ◽  
Dyeing Process ◽  
Colour Strength

The main goal of this study is to modify cotton as cellulose-based fabrics through cationization to improve its dyeing with acid dyes and its antibacterial. Quat-188 was applied to cotton to prepare cationized cotton, overcoming the negative charges between cotton and acid dyes during the dyeing process without using any electrolyte via the pad-dry-cure method. Then the cationized cotton fabrics were treated with the prepared silver nanoparticles to improve their antibacterial properties. The untreated and treated cotton fabrics were dyed with two acid dyes Acid Brilliant Blue PB 100% (acid blue 25; AB25) and Acid Metanil Yellow MT 100% (acid yellow 36) at concentrations of 2%, 4%, and 6% of by exhaust method. Colour strength, color, and washing fastness of untreated and treated cotton fabrics were studied. Antibacterial properties of fabrics were also evaluated against S. aureus and E. coli by using the disk diffusion method. Dyeing properties showed that the treated cotton fabrics significantly improved color strength and fastness properties (light, washing, perspiration, and rubbing). Also, the antibacterial properties of treated cotton fabrics showed antibacterial activity towards tested bacteria. This study reveals that modified cotton fabrics via cationization with Quat-188 and AgNPs have multifunctional properties from their ability for acid dyes and their higher antibacterial activity towards Gram-positive and Gram-negative bacteria that is can be used in many applications.

scholarly journals Evaluation of anti bacteria effects of citrate-reduced silver nanoparticles in Staphylococcus aureus and Escherichia coli

2019 ◽  
Vol 10 (4) ◽  
pp. 3636-3643
Author(s):  
Hor Jia Wei ◽  
Mohd. Syafiq Awang ◽  
Nor Dyana ◽  
Daruliza Kernain ◽  
Yazmin Bustami
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Antibacterial Agent ◽  
Reduction Method ◽  
Surface Plasmon Resonance Peak ◽  
Plasmon Resonance Peak ◽  
Vis Spectroscopy ◽  
Mic Value

Silver nanoparticles (AgNPs) has long known for its inhibitory and bactericidal effects. However, due to its’ attractive antibacterial property, on-going research with various synthesis strategies actively been conducted. In this study, the synthesis of AgNPs was reported, using a simple chemical reduction method with citrate as the reducing agent TEM was used to characterize the obtained AgNPs. Then, Staphylococcus aureus and Escherichia coli were used to identify the antibacterial activity of AgNPs. The inhibition effects of AgNPs against these two bacteria were observed via disc diffusion, and MIC assays and the effects of AgNPs mode of action on both bacteria were further observed under TEM. The formation of AgNPs at ̴ 400 nm, which is the surface plasmon resonance peak was observed using Uv-Vis spectroscopy. The size of AgNPs mostly in the range of 1-10 nm and their morphology appeared to be spherical. Based on the MIC assay, Escherichia coli exhibit low MIC value with 0.049 mg/ml as compared to Staphylococcus aureus with0.391 mg/ml MIC value; correspond to the effective antibacterial activity by the citrate-reduced AgNPs. Further observation on the bacterial surface structure can be seen with cross-sectional TEM image, and it provides an insight into the AgNPs mechanistic aspects of AgNPs against Staphylococcus aureus and Escherichia coli. Silver nanoparticles have been successfully synthesised using the citrate reduction method. Results obtained in this study thus elucidating promising findings to employed AgNPs as an antibacterial agent, and this composition needs to be further study and develop into an antibacterial agent.

Green synthetic approach for starch capped silver nanoparticles and their antibacterial activity

2016 ◽  
Vol 88 (1-2) ◽  
pp. 61-69 ◽  
Author(s):  
Precious Nokwethemba Sibiya ◽  
Thokozani Xaba ◽  
Makwena Justice Moloto
Keyword(s):  
Silver Nanoparticles ◽  
Synthesis Method ◽  
Spherical Shape ◽  
Cubic Phase ◽  
Hydroxyl Groups ◽  
Synthetic Approach ◽  
Surface Plasmon Resonance Peak ◽  
Plasmon Resonance Peak ◽  
Transmission Electron ◽  
Average Size

AbstractIn this work silver nanoparticles (AgNPs) have been prepared from silver nitrate (AgNO3) precursor using a green synthesis method at room temperature. Starch with its abundance of hydroxyl groups and its biocompatibility was used as a capping and reducing agent. The formation of AgNPs was confirmed by absorption spectroscopy with the surface plasmon resonance peak at 400 nm. The sharp reflection at (111), (200), (220) and (311) was observed by powder X-ray diffraction (XRD), which indicated the presence of cubic phase AgNPs. Transmission electron microscopy (TEM) revealed that the average size of AgNPs were between 0.5 and 4 nm with a spherical shape under optimum conditions. The nanoparticles showed a decrease in size with an increase in precursor concentration as well as the increase in capping agent concentration. The nanoparticles also showed to be bactericidal towards the tested Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria.

scholarly journals Antimicrobial activities of biosynthesized silver nano particles from leaves extract of some medicinal plants

2021 ◽  
Vol 14 (2) ◽  
pp. 197-203
Author(s):  
Samuel J. Offor ◽  
Victor U. Anah ◽  
Romanus Umoh ◽  
Uduak P. Iseh ◽  
Idara I. Ebong ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Medicinal Plants ◽  
Visual Inspection ◽  
Mangifera Indica ◽  
Nano Particles ◽  
Diffusion Method ◽  
Surface Plasmon Resonance Peak ◽  
Plasmon Resonance Peak ◽  
Golden Yellow

The antimicrobial activity of biosynthesized silver nanoparticles (AgNPs) from the leaves of four medicinal plants, Carica papaya (CP), Moringa oleifera (MO), Mangifera indica (MI) and Garcinia kola (GK) were assessed against selected gram positive and gram-negative bacteria. Method of synthesis of nanoparticles utilized was the eco-friendly Bio-based method using plant leaves extract as reducing and stabilizing agents. Two different ratios for each plant extract and silver nitrate (1:1 and 1:2) respectively were used. Particle characterization was carried out using visual inspection and UV-Vis spectrophotometry. Antimicrobial activity was assessed using agar diffusion method. Visual inspection revealed gradual color change from golden yellow to dark brown, confirming nanoparticles formation. The surface plasmon resonance peak was between 416 and 438 nm for the silver nanoparticles. The minimum inhibitory concentration ranged from 3.125 - 12.5µg/ml. In conclusion, all four biogenic silver nanoparticles have reasonable antimicrobial activity with ratio 1:2 being more potent.

scholarly journals Green Fabrication of Silver Nanoparticles by Gum Tragacanth (Astragalus gummifer): A Dual Functional Reductant and Stabilizer

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Aruna Jyothi Kora ◽  
Jayaraman Arunachalam
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Water Soluble ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
X Ray Diffraction ◽  
Gum Tragacanth ◽  
Synthesis Of Nanoparticles ◽  
Dual Functional ◽  
Transmission Electron

A simple and ecofriendly procedure have been devised for the green synthesis of silver nanoparticles using the aqueous extract of gum tragacanth (Astragalus gummifer), a renewable, nontoxic natural phyto-exudate. The water soluble components in the gum act as reductants and stabilizers. The generated nanoparticles were analyzed using UV-visible spectroscopy, transmission electron microscopy, X-ray diffraction, Fourier transform-infrared spectroscopy, and Raman spectroscopy. The role of gum concentration and reaction time on the synthesis of nanoparticles was studied. By regulating the reaction conditions, spherical nanoparticles of13.1±1.0 nm size were produced. Also, the possible functional groups involved in reduction and capping of nanoparticles has been elucidated. The antibacterial activity of the fabricated nanoparticles was tested on model Gram-negative and Gram-positive bacterial strains with well-diffusion method. These nanoparticles exhibited considerable antibacterial activity on both the Gram classes of bacteria, implying their potential biomedical applications.

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