scholarly journals Engineered Hypopharynx from Coculture of Epithelial Cells and Fibroblasts Using Poly(ester urethane) as Substratum

2013 ◽  
Vol 2013 ◽  
pp. 1-11 ◽  
Author(s):  
Zhisen Shen ◽  
Jingjing Chen ◽  
Cheng Kang ◽  
Changfeng Gong ◽  
Yabin Zhu
Keyword(s):  
Tissue Engineering ◽  
Epithelial Cells ◽  
Silk Fibroin ◽  
Porous Scaffold ◽  
Engineering Research ◽  
Polymeric Scaffolds ◽  
Cell Tissue ◽  
Silkworm Cocoon

Porous polymeric scaffolds have been much investigated and applied in the field of tissue engineering research. Poly(ester urethane) (PEU) scaffolds, comprising pores of 1–20 μm in diameter on one surface and ≥200 μm on the opposite surface and in bulk, were fabricated using phase separation method for hypopharyngeal tissue engineering. The scaffolds were grafted with silk fibroin (SF) generated from natural silkworm cocoon to enhance the scaffold’s hydrophilicity and further improve cytocompatibility to both primary epithelial cells (ECs) and fibroblasts of human hypopharynx tissue. Coculture of ECs and fibroblasts was conducted on the SF-grafted PEU scaffold (PEU-SF) to evaluate itsin vitrocytocompatibility. After co-culture for 14 days, ECs were lined on the scaffold surface while fibroblasts were distributed in scaffold bulk. The results ofin vivoinvestigation showed that PEU porous scaffold possessed good biocompatibility after it was grafted by silk fibroin. SF grafting improved the cell/tissue infiltration into scaffold bulk. Thus, PEU-SF porous scaffold is expected to be a good candidate to support the hypopharynx regeneration.

scholarly journals Synthesis and Properties of Flexible Polyurethane Using Ferric Catalyst for Hypopharyngeal Tissue Engineering

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Zhisen Shen ◽  
Jian Wang ◽  
Dakai Lu ◽  
Qun Li ◽  
Chongchang Zhou ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Matrix Material ◽  
Biological Properties ◽  
Hexamethylene Diisocyanate ◽  
Engineering Research ◽  
Biodegradable Polyurethane ◽  
Flexible Polyurethane ◽  
Hydrophilic Polyurethane

Biodegradable polyurethane is an ideal candidate material to fabricate tissue engineered hypopharynx from its good mechanical properties and biodegradability. We thus synthesized a hydrophilic polyurethane via reactions among polyethylene glycol (PEG), e-caprolactone (e-CL) and hexamethylene diisocyanate (HDI), and thrihydroxymethyl propane (TMP). The product possessed a fast degradability due to its good wettability and good mechanical parameters with the elongations at break (137 ± 10%) and tensile strength (4.73 ± 0.46 MPa), which will make it a good matrix material for soft tissue like hypopharynx. Its biological properties were evaluated viain vitroandin vivotests. The results showed that this hydrophilic polyurethane material can support hypopharyngeal fibroblast growth and owned good degradability and low inflammatory reaction in subcutaneous implantation. It will be proposed as the scaffold for hypopharyngeal tissue engineering research in our future study.

scholarly journals Meniscal Regenerative Scaffolds Based on Biopolymers and Polymers: Recent Status and Applications

2021 ◽  
Vol 9 ◽  
Author(s):  
Hao Li ◽  
Pinxue Li ◽  
Zhen Yang ◽  
Cangjian Gao ◽  
Liwei Fu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Regenerative Process ◽  
Molecular Signaling ◽  
Temporal Regulation ◽  
Innovative Strategies ◽  
Meniscal Tissue ◽  
Polymeric Scaffolds ◽  
Multiple Challenges

Knee menisci are structurally complex components that preserve appropriate biomechanics of the knee. Meniscal tissue is susceptible to injury and cannot heal spontaneously from most pathologies, especially considering the limited regenerative capacity of the inner avascular region. Conventional clinical treatments span from conservative therapy to meniscus implantation, all with limitations. There have been advances in meniscal tissue engineering and regenerative medicine in terms of potential combinations of polymeric biomaterials, endogenous cells and stimuli, resulting in innovative strategies. Recently, polymeric scaffolds have provided researchers with a powerful instrument to rationally support the requirements for meniscal tissue regeneration, ranging from an ideal architecture to biocompatibility and bioactivity. However, multiple challenges involving the anisotropic structure, sophisticated regenerative process, and challenging healing environment of the meniscus still create barriers to clinical application. Advances in scaffold manufacturing technology, temporal regulation of molecular signaling and investigation of host immunoresponses to scaffolds in tissue engineering provide alternative strategies, and studies have shed light on this field. Accordingly, this review aims to summarize the current polymers used to fabricate meniscal scaffolds and their applications in vivo and in vitro to evaluate their potential utility in meniscal tissue engineering. Recent progress on combinations of two or more types of polymers is described, with a focus on advanced strategies associated with technologies and immune compatibility and tunability. Finally, we discuss the current challenges and future prospects for regenerating injured meniscal tissues.

ADDITION OF CONDUCTIVE ELEMENTS TO POLYMERIC SCAFFOLDS FOR MUSCLE TISSUE ENGINEERING

Nano LIFE ◽  
2012 ◽  
Vol 02 (03) ◽  
pp. 1230011 ◽  
Author(s):  
K. D. MCKEON-FISCHER ◽  
J. W. FREEMAN
Keyword(s):  
Tissue Engineering ◽  
Muscle Tissue ◽  
Cardiac Tissue ◽  
Au Nanoparticles ◽  
Future Research ◽  
Electrically Conductive ◽  
Polymeric Scaffolds ◽  
In Vitro Data

Cardiac and skeletal muscles are two tissues that would benefit from an electrically conductive scaffold to regenerate lost or lower functioning areas. By augmenting polymeric scaffolds with conductive elements, the contractile process for both muscles could increase. In this review, the components reviewed include polyaniline (PANi), gold (Au) nanoparticles, and carbon nanotubes (CNT). PANi has been combined with several polymers and increased the conductivity of the scaffolds. It is biocompatible, but increases mechanical properties and decreases scaffold elongation. Tissue engineering using nanoparticles is an emerging area and considerable research focuses on determining possible toxicity due to nanoparticle concentration. Contradicting data exists for both Au nanoparticles and CNT. Smaller Au nanoparticles damage cardiac tissue in vivo while larger ones do not. By comparison, in vitro data shows no harmful results for skeletal muscle cells. Data for CNT is just as diverse as the amount, orientation and further purification or functionalization could all play a role in determining biocompatibility. Future research should focus on establishing the conductivity level needed for each muscle tissue to ascertain the amount of conductive element needed so the most suitable one can be utilized.

IN VITRO AND IN VIVO DEGRADATION OF A TWISTED SILK FIBROIN–POLY(LACTIC-CO-GLYCOLIC ACID) FIBER COMPOSITE ROPE-LIKE SCAFFOLD AND CHANGES IN ITS MECHANICAL PROPERTIES

2016 ◽  
Vol 16 (04) ◽  
pp. 1650053
Author(s):  
WENYUAN ZHANG ◽  
YADONG YANG ◽  
KEJI ZHANG ◽  
YING LI ◽  
GUOJIAN FANG
Keyword(s):  
Mechanical Properties ◽  
Mechanical Property ◽  
Tissue Engineering ◽  
Mass Loss ◽  
Silk Fibroin ◽  
Glycolic Acid ◽  
Property Loss ◽  
In Vivo Degradation

Natural silk fibroin fiber is slowly degraded, which makes it difficult to be replaced quickly by regenerating tissues of tissue engineering. We used poly(lactic-co-glycolic acid) (PLGA, lactic acid:glycolic acid [Formula: see text] 10:90) fibers to adjust the overall degradation rate of the scaffolds. This study fabricated a three-strand helical composite rope-like scaffold from silk fibroin and PLGA fibers (silk fibroin:PLGA [Formula: see text] 36:64) using a twisting method. In vitro and in vivo degradation experiments were performed over 16 weeks. Results suggest that the in vitro and in vivo degradation tendencies of the scaffold were similar, with mass loss lagging behind mechanical property loss. The speed of degradation in vivo was faster than that in vitro. Mechanical property loss of the scaffold was fast during the first three weeks, when mass loss was slow. Mass loss rate accelerated from weeks 3 to 8. The mass and mechanical properties were relatively stable from 8 to 16 weeks. After 16 weeks of degradation, the scaffold still had considerably strong mechanical properties. The scaffold showed a reasonable and suitable degradation speed with good histocompatibility for ligament tissue engineering.

Eggshell Membrane Protein Modified Silk Fibroin-Poly Vinyl Alcohol Scaffold for Bone Tissue Engineering: In Vitro and In Vivo Study

2017 ◽  
Vol 32 ◽  
pp. 69-81 ◽  
Author(s):  
Mahesh Kumar Sah ◽  
Indranil Banerjee ◽  
Krishna Pramanik
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Silk Fibroin ◽  
Tissue Growth ◽  
Eggshell Membrane ◽  
Poly Vinyl Alcohol ◽  
Porous Network

There is a need for high performance scaffold in tissue engineering. Keeping this perspective in mind, the present study delineates the preparation and physico-chemical characterization of soluble eggshell protein (SEP) modified silk fibroin (SF)-polyvinyl alcohol (PVA) scaffold and its application in bone tissue engineering. The SF/PVA scaffold were prepared by salt leaching and modified with eggshell protein. Micro-architechture and porosity analysis revealed that all the scaffolds were having desired pore size (230-360 µm), interconnected porous network and 90% porosity. The scaffolds were found with suitable swelling behavior and biodegradability to support cell proliferation till replaces native osseous tissue. In vitro cyto-compatibility and differentiation study showed that SEP(SF-PVA) supports viability , proliferation and differentiation of cord blood derived human mesenchymal stem cell. Further, in vivo study in mice model showed that the scaffolds are non-immunogenic and support tissue growth. In conclusion, SEP modified SF-PVA scaffold could be a better option for tissue engineering.

scholarly journals In vitro and in vivo evaluation of silk fibroin-hardystonite-gentamicin nanofibrous scaffold for tissue engineering applications

2020 ◽  
Vol 91 ◽  
pp. 106698 ◽  
Author(s):  
Zhina Hadisi ◽  
Hamid Reza Bakhsheshi-Rad ◽  
Tavia Walsh ◽  
Mohammad Mehdi Dehghan ◽  
Saeed Farzad-Mohajeri ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Silk Fibroin ◽  
Nanofibrous Scaffold ◽  
Engineering Applications ◽  
In Vivo Evaluation

Development of a Hydroxyapatite Bone Tissue Engineering Scaffold with a Trimodal Pore Structure

2007 ◽  
Vol 361-363 ◽  
pp. 931-934 ◽  
Author(s):  
Conor T. Buckley ◽  
K.U. O’Kelly
Keyword(s):  
Tissue Engineering ◽  
Cell Viability ◽  
Porous Scaffold ◽  
Tissue Engineering Scaffold ◽  
Porous Scaffolds ◽  
Cell Penetration ◽  
Nutrient Delivery ◽  
And Migration

Tissue-engineering scaffold-based strategies have suffered from limited cell depth viability when cultured in vitro, with viable cells existing within the outer 250-500μm from the fluid-scaffold interface. This is primarily believed to be due to the lack of nutrient delivery into and waste removal from the inner regions of the scaffold construct. Other issues associated with porous scaffolds involve poor seeding efficiencies and limited cell penetration resulting in heterogeneous cellular distributions. This work focuses on the development a novel hydroxyapatite multi-domain porous scaffold architecture (i.e. a scaffold providing a discrete domain for cell occupancy and a separate domain for nutrient delivery) with the specific objectives of embodying in one scaffold the structures required to optimise cell seeding, cell proliferation and migration and potentially to facilitate vascularisation once implanted in vivo. This paper presents the development of the multidomain architecture and preliminary results on cell viability which show a significant improvement in cell viability in the scaffold interiors.

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