The effect of medicinal syrup made from silkworm cocoon on mixed anxiety-depression disorder: a triple-blind randomized clinical trial

Objective: Silkworm cocoon produced by silkworms with the scientific name of Bombyx mori L. is a well-known medicinal agent mainly composed of proteins. This study was designed to assess the efficacy of syrup made from this natural agent on mild to moderate depression. Design: The study was designed as a triple blind randomized clinical trial (RCT). Sixty patients with mixed anxiety–depressive disorder (MADD) were randomized to receive either intervention (silk syrup plus sertraline) or control (placebo syrup + sertraline) for a period of 12 weeks. Depression and anxiety were assessed by using the Beck depression and anxiety inventories at weeks 0, 6 and 12. Results: Fifty-four patients completed the trial in two groups of intervention (n = 30) and control (n = 24) and entered final analysis. According to the results of this study, the mean of anxiety and depression in weeks 6 and week 12 in the intervention group was significantly lower than the control group (p<0.001). Conclusion: Silk syrup can be beneficial for management of anxiety and depression in mild to moderate MADD. Thus, it is suggested as an adjuvant treatment to increase efficacy of conventional drugs in MADD patients.

A Study On The Sericulture in Turkey

Sericulture, or silk farming, is the cultivation of silkworms to produce silk. Although there are several commercial species of silkworms, Silk was believed to have first been produced in China as early as the Neolithic Period. Sericulture has become an important cottage industry in countries such as Brazil, China, France, India, Italy, Japan, Korea, and Russia. Today, China and India are the two main producers, with more than 60% of the world's annual production. In this case, the sericulture production in Turkey, as the export-oriented shows could increase in the future. Sericulture production was begun in Anatolia which is Asian part of Turkey in A.D. 552 during Byzantium Emperor of Justinianus. The city of Bursa became a textile city which was famous for silk and silk trade centre. In middle of 16th silk textile industry was developed. Apart from city of Bursa, cities of Istanbul, dirne, Amasya, Denizli, Izmir and Konya were important sericulture centers In addition; the increase of government support in sericult production is also hope for the future of sericulture production in Turkey. In this study, using the silkworm cocoon data between the years of 1991-2019, the future 5-year production is estimated. For this, estimates were tested in the MINITAB program using the ARIMA Model, which is widely used in agricultural production estimates. Unconscious use of pesticides in Turkey, problems with mulberry tree cultivation, technical problems and rural-urban migration results are known to be important issues of the sericulture sector.However, thanks to government subsidies and export-oriented policies, it has been determined that silkworm cocoon production will increase in the next 5 years.

Insights into detailed hierarchical puncture-resistance for Bombyx mori silkworm cocoons

Silkworm cocoons illustrate excellent puncture-resistance performance after an insight into their layers while a clear understanding of the correlation between the excellent puncture property and the silk secondary structure is still lacking. Herein, we peeled silkworm cocoon into eight layers, and a combination of examination techniques including scanning electron microscopy, tensile mechanical test, Fourier transform infrared spectroscopy, Raman spectroscopy, and X-ray diffraction were applied to figure out the morphologies (surface and cross-section view), mechanical properties, secondary structure, the content of β-sheet, and crystallinity of each layer's fibroin after degumming. The results indicated that the fifth layer offers a higher level of puncture-resistance than the other layers except for the eighth layer. Additionally, a high content level of β-sheet structure and high crystallinity gives rise to the high puncture strength as for hierarchical silk fibers. In general, the new finding holds great potential inspiration for the design of puncture-resistant composites.

Effects of Fabrication Conditions on Structure and Properties of Mechanically Prepared Natural Silk Web and Non-Woven Fabrics

In this study, natural silk web and natural silk non-woven fabric were prepared mechanically using the binding character of the sericin in silk. The effect of process variables on the preparation, structure, and properties of the silk web and the non-woven fabric was examined. The reeling velocity affected the morphology and mechanical properties of the web but had almost no influence on the crystalline structure of the silk. From the viewpoint of reel-ability and the mechanical properties (work of rupture) of silk web, a reeling velocity of 39.2 m/min represented the optimal processing velocity. The porosity and swelling ratio of the silk web decreased slightly with increasing reeling velocity. Furthermore, the reeling bath temperature had a significant effect on the reel-ability of silk filaments from a silkworm cocoon. Bath temperatures ≥50 °C yielded good reel-ability (>900 m reeling length). The porosity, swelling ratio in water, and mechanical properties of the silk web and silk non-woven fabric changed only slightly with the reeling bath temperature but changed significantly with the hot press treatment. The hot-pressed silk web (i.e., silk non-woven fabric) exhibited higher tensile strength as well as lower elongation at break, porosity, and swelling ratio than the silk web.

Sericin based nanoformulations: a comprehensive review on molecular mechanisms of interaction with organisms to biological applications

Background The advances in products based on nanotechnology have directed extensive research on low-cost, biologically compatible, and easily degradable materials. Main body Sericin (SER) is a protein mainly composed of glycine, serine, aspartic acid, and threonine amino acids removed from the silkworm cocoon (particularly Bombyx mori and other species). SER is a biocompatible material with economic viability, which can be easily functionalized due to its potential crosslink reactions. Also, SER has inherent biological properties, which makes possible its use as a component of pharmaceutical formulations with several biomedical applications, such as anti-tumor, antimicrobials, antioxidants and as scaffolds for tissue repair as well as participating in molecular mechanisms attributed to the regulation of transcription factors, reduction of inflammatory signaling molecules, stimulation of apoptosis, migration, and proliferation of mesenchymal cells. Conclusion In this review, the recent innovations on SER-based nano-medicines (nanoparticles, micelles, films, hydrogels, and their hybrid systems) and their contributions for non-conventional therapies are discussed considering different molecular mechanisms for promoting their therapeutic applications.

Structural stability of biofilms produced from silkworm cocoon fibers

Biofilms were obtained from cocoons of the silkworm, Bombyx mori, involving the removal of sericin, extraction and solubilization of fibroin fibers, dialysis of fibroin dispersions and preparation of biofilms by the casting process. Biofilm transparency was verified by UV-Vis spectroscopy and thermal stability by thermogravimetric/differential scanning calorimetry (TG/DSC). Soon after preparation, the solidification of the fibroin solution prepared from the cocoons and extracted by the Ajisawa method was monitored until the biofilm stabilized, using Attenuated Total Reflectance-Fourier Transform Infrared spectroscopy (ATR FT-IR) as a function of time. The results showed that there is a change in the conformation from the silk I structure (?-helix) to silk II (?-sheet). In order to improve the characterization of the biofilms obtained by the Ajisawa method and LiBr solubilization of fibroin fibers, Raman spectroscopy was used to verify stabilization of the different possible molecular conformations for the fibers in these materials, by comparison with the cocoon spectra and those of the solid (freeze-dried hydrogel) precipitated by dialysis for 72 h. By comparing the Raman spectra of the biofilms in terms of the intensities of the broadened band characteristic of amide I, it was possible to assess the conformational changes in both materials based on possible transitions between ?-sheet conformations and flexible ?-helix and ?-turn structures. The results showed a dispersion of these conformations in the biofilms generated and in the solid freeze-dried hydrogel spectrum, and the ?-sheet conformation was found to be predominant. The TG and DSC curves showed that the materials with higher ?-sheet content exhibited higher thermal resistance. Thus, the data obtained further elucidate the properties of these materials which are widely used in various processes.