scholarly journals Antihyperlipidemic Effects ofSesamum indicumL. in Rabbits Fed a High-Fat Diet

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Sedigheh Asgary ◽  
Mahmoud Rafieian-Kopaei ◽  
Somayeh Najafi ◽  
Esfandiar Heidarian ◽  
Amirhossein Sahebkar
Keyword(s):  
High Fat Diet ◽  
Rabbit Model ◽  
Sesame Seed ◽  
Serum Levels ◽  
Diet Group ◽  
Normal Diet ◽  
Sesame Oil ◽  
High Fat ◽  
Hepatic Transaminases ◽  
Hypercholesterolemic Diet

The present study aimed to investigate the anti-hyperlipidemic effects of sesame in a high-fat fed rabbit model. Animals were randomly divided into four groups of eight animals each for 60 days as follows: normal diet, hypercholesterolemic diet (1% cholesterol), hypercholesterolemic diet (1% cholesterol) + sesame seed (10%), and hypercholesterolemic diet (1% cholesterol) + sesame oil (5%). Serum concentrations of total cholesterol, LDL-C, HDL-C, triglycerides, apoA and apoB, SGOT, SGPT, glucose and insulin were measured at the end of supplementation period in all studied groups. Hypercholesterolemic feeding resulted in a significant elevation of TC, TG, LDL-C, HDL-C, SGOT and SGPT as compared to the normocholesterolemic diet group (P<0.05). Supplementation with sesame seed did not cause any significant alteration in lipid profile parameters, apolipoproteins, hepatic transaminases, glucose and insulin as compared to the hypercholesterolemic diet group (P>0.05). In contrast, rabbits supplemented with sesame oil were found to have lower circulating concentrations of TC, LDL-C, HDL-C, SGOT and SGPT (P<0.05), whilst concentrations of TG, apoA, apoB, insulin and glucose remained unaltered compared to the hypercholesterolemic diet group (P>0.05). Supplementation with sesame oil, but not sesame seed, can ameliorate serum levels of lipids and hepatic enzymes in rabbits under a high-fat diet.

Effects of Zinc Alpha2 Glycoprotein on Lipid Metabolism of Liver in High-Fat Diet-Induced Obese Mice

2017 ◽  
Vol 49 (10) ◽  
pp. 793-800 ◽  
Author(s):  
Guoqiang Fan ◽  
Yu Qiao ◽  
Shixing Gao ◽  
Jun Guo ◽  
Ruqian Zhao ◽  
...  
Keyword(s):  
Body Weight ◽  
Lipid Metabolism ◽  
High Fat Diet ◽  
Recombinant Plasmid ◽  
Diet Group ◽  
Normal Diet ◽  
Hormone Sensitive Lipase ◽  
High Fat ◽  
Hepatic Lipid ◽  
Protein Levels

AbstractZinc alpha2 glycoprotein (ZAG) is a new type of adipokine involved in adipose tissue mobilization, however, little is known about its lipid metabolism effect in liver. Therefore, we investigated the effects of ZAG in the regulation of hepatic lipid accumulation. Mice were randomly divided into two groups; one was fed a normal diet and another was fed a high-fat diet for eight weeks to establish obesity model. After that, the normal diet group was divided into ND (injection of pcDNA3.1) and NDZ (injection of ZAG recombinant plasmid) and the high-fat diet group was divided into HF (injection of pcDNA3.1) and HFZ (injection of ZAG recombinant plasmid). The mice were weighed once per week and injected with plasmid once every three days for eight times. The results showed that body weight and hepatic TG content were decreased dramatically in HFZ group compared with HF group. The stearoyl-CoAdesaturase1 (SCD1) and Acyl-CoA Synthetase-1 (ACSS1) protein levels in HFZ group were significantly decreased. Furthermore, phosphorylated hormone sensitive lipase (P-HSL) was significantly higher in HFZ group. In HFZ group, hepatic fatty acid translocase (CD36) and fatty acids binding protein-1 (FABP1) protein levels were reduced. In addition, the expression of phosphorylated protein kinase A (PPKA) in HFZ group was higher than the HF group. Meanwhile, NDZ group showed significantly decreased body weight and increased P-HSL level though the hepatic TG content showed no significantly changes compared with the ND group. Therefore, we conclude that ZAG may be beneficial for preventing high-fat-diet-induced hepatic lipid metabolic disorders.

scholarly journals Regulating AMPKα and insulin level by Vinegar, Swimming and Refeeding on High-Fat Diet Rats to Rebuild Lipid Homeostasis

2020 ◽  
Author(s):  
Yuan Yang ◽  
Feng Zhang ◽  
Xiao Xiao ◽  
Chunlian Ma ◽  
Hua Liu ◽  
...  
Keyword(s):  
Weight Gain ◽  
High Fat Diet ◽  
Insulin Level ◽  
Diet Group ◽  
Normal Diet ◽  
Lipid Homeostasis ◽  
Control Group ◽  
High Fat ◽  
Better Regulation ◽  
Rice Vinegar

AbstractOur aims were to explore the effects of dietary and behavior interventions on lipometabolism caused by unhealthy high-fat diet and the best method to rebuild lipid homeostasis of this lifestyle. Apart from normal diet rats, 34 rats were fed with high-fat emulsion for 4 weeks before being divided into 4 groups and intervened for another 4 weeks. 8 of them were classified into high-fat control group and 9 were sorted into high-fat diet with rice vinegar group. Meanwhile, 10 were put into high-fat diet with swimming group and 7 were just for refeeding normal diet group. Then the data of body weight was recorded and analyzed. Serum, pancreas, liver, cardiac tissues and epididymis adipose were sampled as required. Indexes of serum were tested by kits. AMPKα, HNF1α, CTRP6 from tissues were detected by western blot. According to our experiments, Swimming and refeeding groups reflected a better regulation on lipid homeostasis mainly by up-regulating the expression of pancreas AMPKα. To be more specific, the refeeding rats showed lower T-CHO (P<0.001) and LDL-C (P<0.05), but higher weight gain (P<0.001),insulin level (P<0.01)and pancreas AMPKα (P<0.01)than high-fat control rats. Compared with rats experimented by swimming or rice vinegar, they showed higher weight gain (P<0.001),insulin level (P<0.01)and HNF1α, but lower of CTRP6. In summary, refeeding diet functioned better in regulating the lipometabolic level after high-fat diet. Whatever approach mentioned above we adopted to intervene, the best policy to keep the balance of lipid homeostasis is to maintain a healthy diet.

scholarly journals THE EFFECT OF INTRAGASTRICALLY ADMINISTERED SESAME (SESAMUM INDICUM L) OIL ON GROWTH RATE AND SOME BIOCHEMICAL PROFILE OF RATS FED A HIGH-FAT DIET.

2021 ◽  
Vol 25 (2) ◽  
pp. 120-128
Author(s):  
J. A. OMAGE ◽  
I. A. UMAR ◽  
G. S. BAWA
Keyword(s):  
Growth Rate ◽  
High Fat Diet ◽  
Seed Oil ◽  
Sesame Seed ◽  
Sesamum Indicum ◽  
Normal Diet ◽  
Biochemical Profile ◽  
High Fat ◽  
Sesame Seed Oil ◽  
Diet Control

The effect(s) of sesame (Sesamum Indicum L) seed oil on the growth rate, performance and some biochemical parameters in adult albino (Wister) rats maintained on a high fat diet was examined. Adult rats maintained on a high fat diet were intragastrically fed 10mg/ml of sesame seed oil suspended in normal saline, daily for 60 days. Two other groups maintained on the high fat diet only (High fat diet control) and the normal diet (Normal diet control) were included. Water and diet were provided ad libitum. The rats were weighed weekly, while feed supply and left-overs were recorded daily. At 60 days, all animals were killed by decapitation. Blood and liver samples were taken for biochemical analyses. Feeding high fat diet significantly (P<0.05) increased the final body weight, liver and kidney weights of the rats compared to those on normal diet. Administration of sesame seed oil along with high fat diet further increased (P <0.05) the final rat body weight while decrease (P<0.05) was observed in the liver weight and a significant (P 0.05) decrease in the kidney weight was observed compared to the high fat diet fed rats. Biochemical profile shows significantly (P<0.01) higher levels of plasma proteins and globulins in the normal diet-fed rats compared to the high fat-diet-fed rats. Administration of sesame seed oil along with high fat diet significantly (P<0.01) increased the plasma glucose, total proteins, globulins and uric acid concentrations compared to the high fat diet fed rats. Significantly (P<0.01) higher glucose and uric acid levels and a significantly (P<0.01) lower globulin levels were observed in the sesame seed oil group compared to the normal diet control group. The plasma albumin concentration did not differ significantly among the groups. 

scholarly journals The Role of Exercise in Reducing Hyperlipidemia-Induced Neuronal Damage in Apolipoprotein E-Deficient Mice

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Yumeng Bai ◽  
Yali Feng ◽  
Bo Jiang ◽  
Yan Yang ◽  
Zuowei Pei ◽  
...  
Keyword(s):  
Exercise Training ◽  
Apolipoprotein E ◽  
Real Time ◽  
Real Time Pcr ◽  
High Fat Diet ◽  
Neuronal Injury ◽  
Serum Levels ◽  
Normal Diet ◽  
High Fat ◽  
Expression Levels

Hyperlipidemia causes nervous system-related diseases. Exercise training has developed into an established evidence-based treatment strategy that is beneficial for neuronal injury. This study investigated the effect of exercise on hyperlipidemia-induced neuronal injury in apolipoprotein E-deficient (ApoE-/-) mice. Male ApoE-/- mice (age: 8 weeks) were randomly divided into four groups as follows: mice fed a normal diet (ND), normal diet+swimming training (ND+S), high-fat diet (HD), and high-fat diet+swimming (HD+S). Exercise training consisted of swimming for 40 min/day, 5 days/week for 12 weeks. After 12 weeks, we measured serum levels of total cholesterol (TC), triglyceride (TG), and low-density lipoprotein cholesterol (LDL-c). We also evaluated glial fibrillary acidic protein (GFAP) expression levels using immunohistochemistry, real-time PCR, and immunoblotting. In addition, NLR family pyrin domain-containing 3 (NLRP3), interleukin- (IL-) 18, caspase-1, Bax, Bcl-2, and phosphorylated extracellular signal-regulated kinase (p-ERK) expression levels were measured using immunoblotting. Serum levels of TG, TC, and LDL-c were lower in ApoE-/- HD+S mice than in ApoE-/- HD mice. Immunohistochemistry, real-time PCR, and immunoblotting showed increased levels of GFAP in the ApoE-/- HD group. Immunoblotting revealed increased levels of NLRP3, IL-18, caspase-1, Bax, Bcl-2, and p-ERK in the ApoE-/- HD group; however, they were significantly suppressed in the ApoE-/- HD+S group. Therefore, exercise has protective effects against neuronal injury caused by hyperlipidemia.

scholarly journals Maternal High-Fat Diet Promotes the Development and Progression of Prostate Cancer in Transgenic Adenocarcinoma Mouse Prostate Offspring

2018 ◽  
Vol 47 (5) ◽  
pp. 1862-1870 ◽  
Author(s):  
Tian Yang ◽  
Xiaobo Wu ◽  
Jimeng Hu ◽  
Mengbo Hu ◽  
Hua Xu ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Mortality Rate ◽  
High Fat Diet ◽  
Formation Rate ◽  
Serum Levels ◽  
Normal Diet ◽  
Tumor Formation ◽  
High Fat ◽  
Mouse Prostate ◽  
The Impact

Background/Aims: We aim to investigate the impact of maternal high fat diet (HFD) on the development and progression of prostate cancer (PCa) in transgenic adenocarcinoma mouse prostate (TRAMP) offspring. Methods: The TRAMP model was used, and divided into maternal HFD group and normal diet (ND) group in the present study. Each group contained 36 TRAMP mice. Serum levels of leptin, adiponectin, interleukin (IL) -1α, IL-1β, IL-6, tumor necrosis factor-α and monocyte chemotactic protein-1 were measured by the 20th, 24th and 28th week old through ProcartaPlex Multiplex Immunoassay. Body fat ratio was measured by MiniQMR. Tumor formation rate was measured through hematoxylin and eosin (H&E) staining, and mortality rate was measured meantime. Western blot was applied to determine the levels of Protein Kinase B (Akt) and Phosphatase and tensin homolog (PTEN). Results: The mortality rate of maternal HFD group was significantly higher than that of ND group (P = 0.046). The tumor formation rate was significantly higher in maternal HFD group than in ND group only in 20th week subgroup (P = 0.040). A significant increase of leptin was seen in maternal HFD 20th and 24th week subgroups (P = 0.001 and < 0.001, respectively) and a decrease of adiponectin was seen in maternal HFD 20th and 28th week subgroups (P =0.006 and < 0.001, respectively). Besides, an activated phos-Akt (P-Akt) and deactivated PTEN were observed in maternal HFD group. Conclusions: Maternal HFD could increase the standard serum leptin level, inhibit the expression of PTEN protein, promote P-Akt protein expression, activate the PI3K/Akt pathway, and ultimately promote the development and progression of PCa in TRAMP offspring.

scholarly journals NMR-Based Metabolomics Approach to Investigate the Effects of Fruits of Acanthopanax sessiliflorus in a High-Fat Diet Induced Mouse Model

Metabolites ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 505
Author(s):  
Bo-Ram Choi ◽  
Dahye Yoon ◽  
Hyoung-Geun Kim ◽  
Seon Min Oh ◽  
Yung Choon Yoo ◽  
...  
Keyword(s):  
Mouse Model ◽  
High Fat Diet ◽  
Diet Group ◽  
Normal Diet ◽  
High Fat ◽  
Multivariate Statistical ◽  
Nmr Spectrometry ◽  
Biomarker Analysis ◽  
Prevalence Of Obesity ◽  
Acanthopanax Sessiliflorus

The prevalence of obesity is rapidly increasing and is recognized as a serious health problem. To investigate metabolic changes in an obese model after administration of Acanthopanax sessiliflorus, mice were divided into four groups: normal diet, high-fat diet (HFD), HFD with treatment fenofibrate, and A. sessiliflorus fruit extract. The liver tissue of mice was analyzed using nuclear magnetic resonance (NMR) spectrometry-based metabolomics. In multivariate statistical analyses, the HFD group was discriminated from the normal diet group, and the group fed A. sessiliflorus fruit was discriminated from the HFD group. In biomarker analysis between the HFD group and the group fed A. sessiliflorus fruit, alanine, inosine, formate, pyroglutamate, taurine, and tyrosine, with AUC values of 0.7 or more, were found. The levels of these metabolites were distinguished from the HFD mouse model. Changes in these metabolites were confirmed to act on metabolic pathways related to antioxidant activity.

scholarly journals Anti-Obese Effect of Cinnamon Extracts Dietary Supplementation on Serum Lipids and Body Weight Gain in High-Fat-Diet Induced Obese Mice Model

2021 ◽  
Vol 5 (Supplement_2) ◽  
pp. 1236-1236
Author(s):  
Joohee Oh ◽  
Hyun-Sook !Kim
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
High Fat Diet ◽  
Body Weight Gain ◽  
Diet Group ◽  
Normal Diet ◽  
Obese Mice ◽  
Mice Model ◽  
High Fat ◽  
Final Body Weight

Abstract Objectives Cinnamon is one of the oldest spices widely used in traditional medicine and also currently used by people all around the world. Cinnamon has been known for modulating metabolic disorders by regulating insulin sensitivity. The aim of this study was to investigate the anti-obese effects of cinnamon extracts in high-fat-diet induced obese mice model. Methods After a week of adaptation period, the 6-week-old male C57BL/6J mice were randomly divided into 4 groups (n = 11 for each group) of the normal diet group (ND), the high-fat-diet group (HF), the normal diet with 1% cinnamon extracts (NC), and the high-fat diet with 1% cinnamon extracts (HC). All groups were treated for 14 weeks. Results In final body weight and body weight gain, NC group was significantly lower than ND group and HC group was significantly lower than HF group (P = 0.000). In serum TG (Triglyceride) levels and TC (Total cholesterol) levels, NC group showed significantly decreased level compared to that of ND group and HC group represented significantly decreased level compared to that of HF group (P = 0.000). Conclusions The present data showed NC group and HC group showed lower final body weight and body weight gain than ND group and HF group. Also, NC group and HC group showed the decreased level of TG (Triglyceride) and TC (Total cholesterol) compared to ND group and HF group. The further indicators of insulin-related factors are in progress. Funding Sources This study received no external funding.

scholarly journals The Influence of Hyperlipidemia on Endothelial Function of FPN1 Tek-Cre Mice and the Intervention Effect of Tetramethylpyrazine

2018 ◽  
Vol 47 (1) ◽  
pp. 119-128 ◽  
Author(s):  
Ming-Yue Sun ◽  
Miao Zhang ◽  
Shui-Ling Chen ◽  
Shu-Ping Zhang ◽  
Chun-Yu Guo ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Endothelial Function ◽  
Iron Overload ◽  
High Fat Diet ◽  
Iron Homeostasis ◽  
Diet Group ◽  
Normal Diet ◽  
High Fat ◽  
Excess Iron ◽  
Serum Hepcidin

Background/Aims: Systemic iron homeostasis is strictly governed in mammals; however, disordered iron metabolism (such as excess iron burden) is recognized as a risk factor for various types of diseases including AS (Atherosclerosis). The hepcidin-ferroportin axis plays the key role in regulation of iron homeostasis and modulation of this signaling could be a potential therapeutic strategy in the treatment of these diseases. TMP (Tetramethylpyrazine) has been reported to have therapeutical effect on AS. Here, we aimed to investigate the effect of iron overload under hyperlipidemia condition on the endothelial injury, inflammation and oxidative stress by employing FPN1 Tek-cre mouse model with or without TMP intervention. Methods: Subjects for this study were 80 FPN1 Tek-cre mice and 40 C57BL/6 mice and we randomly divided them into six groups: Group N: C57BL/6 mice with normal diet, Group M: C57BL/6 mice with high-fat diet, Group FN: FPN1 Tek-cre mice with normal diet, Group FNT: FPN1 Tek-cre mice with normal diet and TMP injection, Group FM: FPN1 Tek-cre mice with high-fat diet, Group FMT: FPN1 Tek-cre mice with high-fat diet and TMP injection. After seven days of treatment, blood samples were obtained to detect the levels of blood lipids, Hepcidin, NO, ET-1, ROS, MDA, SOD, IL-1, IL-6 and TNF-α respectively. The liver and aorta were used for testing the lipid deposition by using hematoxylin and eosin(HE). Results: Hyperlipidemia could cause iron overload in the aorta and increased serum hepcidin level, particularly in FPN1 Tek-cre mice, and can be reversed by TMP intervention. Knockout of Fpn1 induced increase of serum hepcidin, exacerbated endothelial dysfunction, oxidative stress and inflammatory response, particularly under hyperlipidemia condition. TMP intervention attenuated these processes. Conclusions: Our study signifies the potential application of certain natural compounds to ameliorating iron disorders induced by hyperlipidemia and protecting on endothelial function through modulation of hepcidin-ferroportin signaling.

scholarly journals Neuroprotective role of Ginkgobiloba and Rosuvastatin in CA1 region of Hippocampus against high fat diet induced neurotoxicity

2021 ◽  
Vol 8 (3) ◽  
pp. 179-189
Author(s):  
Anil Kumar ◽  
Anand Acharya ◽  
Subhadra Devi Velichety ◽  
Rajesh Vaderav
Keyword(s):  
Body Weight ◽  
Spatial Learning ◽  
High Fat Diet ◽  
Diet Group ◽  
Normal Diet ◽  
High Fat ◽  
Ca1 Region ◽  
Group I ◽  
Branching Points

Diet rich in fat is one of the main risk factor for the development of Alzheimer’s disease. Studies have shown that diet rich in fat disrupts memory and learning. The present study evaluates the ameliorative role of Ginkgobiloba and Rosuvastatin against high fat diet induced neurotoxicity in CA1 (Corona Ammonis) region of hippocampus. Animals were randomly divided into six groups. Group I received normal diet, Group II received high fat diet, Group III & IV were treated with Ginkgobiloba 50mg/kg and 100mg/kg body weight, and Group V & VI were treated with Rosuvastatin 10mg/kg and 20 mg/kg body weight. All the rats were subjected to spatial learning (Morris water maze). Subsequently, rats were sacrificed and brains were removed. Golgi staining was done and CA1 neurons of hippocampus were traced using camera lucida. Dendritic branching points and dendritic intersections were quantified. Lipid profile and Super oxide (SOD) was also estimated.There was enhancement of spatial learning in treatment group rats. Furthermore, a significant increase in dendritic length and branching points was observed in CA1 region along with significant decrease in the Superoxide dismutase in rats treated with higher dose of Ginkgobiloba and Rosuvastatin. Present study concludes that Ginkgobiloba and Rosuvastatin in higher dose have protective role against high fat diet induced neurotoxicity in CA1 region.

scholarly journals Proinflammatory Cytokines in Prostate Cancer Development and Progression Promoted by High-Fat Diet

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Hua Xu ◽  
Meng-bo Hu ◽  
Pei-de Bai ◽  
Wen-hui Zhu ◽  
Sheng-hua Liu ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Mortality Rate ◽  
Proinflammatory Cytokines ◽  
High Fat Diet ◽  
Formation Rate ◽  
Diet Group ◽  
Normal Diet ◽  
Tumor Formation ◽  
High Fat ◽  
Tramp Mice

Background. We aimed to examine whether proinflammatory cytokines participated in prostate cancer (PCa) development and progression promoted by high-fat diet (HFD).Methods. TRAMP (transgenic adenocarcinoma mouse prostate) mice were randomly divided into two groups: normal diet group and HFD group. Mortality rate and tumor formation rate were examined. TRAMP mice were sacrificed and sampled on the 20th, 24th, and 28th week, respectively. Levels of proinflammatory cytokines, including IL-1α, IL-1β, IL-6, and TNF-α, were tested by FlowCytomix. Prostate tissue of TRAMP mice was used for histology study.Results. A total of 13 deaths of TRAMP mice were observed, among which 3 (8.33%) were from the normal diet group and 10 (27.78%) from the HFD group. The mortality rate of TRAMP mice from HFD group was significantly higher than that of normal diet group (P=0.032). Tumor formation rate at 20th week of age of HFD group was significantly higher than that of normal diet group (P=0.045). Proinflammatory cytokines levels, including IL-1α, IL-1β, IL-6, and TNF-α, were significantly higher in HFD TRAMP mice.Conclusions. HFD could promote TRAMP mouse PCa development and progression with elevated proinflammatory cytokines levels. Proinflammatory cytokines could contribute to PCa development and progression promoted by HFD.

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