scholarly journals Efficient IDUA Gene Mutation Detection with Combined Use of dHPLC and Dried Blood Samples

2013 ◽  
Vol 2013 ◽  
pp. 1-4 ◽  
Author(s):  
Diogo Ribeiro ◽  
Ana Cardoso ◽  
Ana Joana Duarte ◽  
Luis Vieira ◽  
Olga Amaral
Keyword(s):  
High Performance ◽  
Gc Content ◽  
Dried Blood Spots ◽  
Technical Requirements ◽  
Combined Use ◽  
Exon 9 ◽  
The Common ◽  
Idua Gene ◽  
The Cost ◽  
Design And Methods

Objectives. Development of a simple mutation directed method in order to allow lowering the cost of mutation testing using an easily obtainable biological material. Assessment of the feasibility of such method was tested using a GC-rich amplicon. Design and Methods. A method of denaturing high-performance liquid chromatography (dHPLC) was improved and implemented as a technique for the detection of variants in exon 9 of the IDUA gene. The optimized method was tested in 500 genomic DNA samples obtained from dried blood spots (DBS). Results. With this dHPLC approach it was possible to detect different variants, including the common p.Trp402Ter mutation in the IDUA gene. The high GC content did not interfere with the resolution and reliability of this technique, and discrimination of G-C transversions was also achieved. Conclusion. This PCR-based dHPLC method is proved to be a rapid, a sensitive, and an excellent option for screening numerous samples obtained from DBS. Furthermore, it resulted in the consistent detection of clearly distinguishable profiles of the common p.Trp402Ter IDUA mutation with an advantageous balance of cost and technical requirements.

scholarly journals RESTORATION OF MACHINE PARTS: CHOICE OF THE METHOD

2020 ◽  
Vol 17 (1) ◽  
pp. 84-97
Author(s):  
A. N. Kotomchin ◽  
A. F. Sinelnikov ◽  
N. I. Korneychuk
Keyword(s):  
High Performance ◽  
Spare Part ◽  
Spare Parts ◽  
Chrome Plating ◽  
Wear Resistant Coatings ◽  
Technical Requirements ◽  
Machine Parts ◽  
The Cost ◽  
Selection Of ◽  
Operating Temperature Range

Introduction. In the process of operation of cars there is a need for quality repairs with minimal time and resources. There is a constant search for ways to reduce the cost of operating cars by reducing the cost of buying original spare parts. One way to reduce the cost of spare parts is the restoration of resource-defining expensive car parts. In this regard, there is a search for the selection of optimal ways to restore machine parts that meet the economic and technical requirements and which have a resource of a new spare part. The purpose of the research is to substantiate the feasibility of using galvanic coatings in particular chrome plating in the restoration of machine parts.Materials and methods. The authors proved a rationale recovery of parts of machines galvanized coating and the method of choice of the rational restoration of car details. Moreover, the authors established that a large number of parts are in the range of 0.1...0.5 mm, as a result, the authors used wear-resistant coatings of electrolytic chrome plating.Results. As a result of optimizing the composition of the existing cold self-regulating electrolyte, the authors expanded the operating temperature range (up to 35 o C), at which it was possible to obtain high-quality coatings with high performance.Discussion and conclusions. The authors conclude that in modern conditions of the car production and repair and for certain nomenclature of resource-defining details, it is possible to use one of the restoration ways, namely, chrome plating. In order to reduce costs and increase the performance of chrome plating, it is necessary to improve it by optimizing the electrolyte composition and electrolysis modes.Financial transparency: the authors have no financial interest in the presented materials or methods. There is no conflict of interest.

Developing the adaptive process-based budgeting through TD-ABC and TD-ABB as part of strategic management accounting

2020 ◽  
Vol 23 (10) ◽  
pp. 1182-1194
Author(s):  
A.A. Akhmetzyanov ◽  
A.Yu. Sokolov
Keyword(s):  
Strategic Management ◽  
Cost Allocation ◽  
Management Accounting ◽  
Practical Significance ◽  
Common Time ◽  
Strategic Management Accounting ◽  
Scientific Papers ◽  
The Subject ◽  
The Common ◽  
The Cost

Subject. The article focuses on the advanced time-driven tools for allocating overhead expenses, which are based on process-based budgeting. Objectives. We articulate a technique for cost allocation so as to assess the cost of each process with reference to the common time driver. Methods. The study relies upon methods of systematization, classification, analogy and comparison, and summarizes the scientific literature on the subject. Results. The article presents our own suggestions on implementing TD-ABC and TD-ABB into the strategic management accounting process of developer companies. The principles were proved to help more effectively allocate overhead expenses and assess the capacity load of each process performed by functions, departments and employees. Carrying out a comparative analysis, we found certain reserves for utilizing resources more effectively. Conclusions and Relevance. The findings are of scientific and practical significance and can be used by developer and construction businesses. The conclusions can prove helpful for scientific papers, student books, and further research.

Development and Validation of a Novel Reversed Phase High Performance Liquid Chromatography with Refractive Index Detector Method for Assay of Polyvinyl Alcohol in an Ophthalmic Solution

2020 ◽  
Vol 16 (7) ◽  
pp. 867-871
Author(s):  
Harun Ergen ◽  
Muge Guleli ◽  
Cigdem Sener ◽  
Cem Caliskan ◽  
Sercan Semiz ◽  
...  
Keyword(s):  
Refractive Index ◽  
Liquid Chromatography ◽  
Polyvinyl Alcohol ◽  
High Performance ◽  
Ophthalmic Solution ◽  
Reversed Phase ◽  
Hplc Method ◽  
Solution Stability ◽  
Technical Requirements ◽  
Refractive Index Detector

Introduction: Polyvinyl alcohol (PVA), a polymer, is in demand due to its usage in different applications such as pharmaceutical, biomedical and textile, paper, food industries. Methods: A new sensitive reversed phased high-pressure liquid chromatography (RP-HPLC) method with refractive index detector (RID) was developed for determination of PVA in an ophthalmic solution containing dexpanthenol and PVA as active substances and it was validated according to The International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use (ICH) guideline. Results: Chromatographic separation was achieved on a Chiral-AGP (150 mm × 4.0 mm, 5 μm) column kept at 30°C with an isocratic flow at a flow rate of 1.0 ml/min. The detector temperature was 30°C, the retention time of PVA was around 1.0 min and the total run time was 5 minutes. Conclusion: The proposed method showed linearity, accuracy, precision, specificity, robustness, solution stability, and system suitability results within the acceptance criteria.

scholarly journals Fast Comparison of High-Precision Time Scales Using GNSS Receivers

2017 ◽  
Vol 2017 ◽  
pp. 1-4
Author(s):  
Vojtech Vigner ◽  
Jaroslav Roztocil
Keyword(s):  
Time Scales ◽  
Time Scale ◽  
High Performance ◽  
Data Format ◽  
Common View ◽  
Log Files ◽  
Gnss Receivers ◽  
Precision Time ◽  
The Common ◽  
The Difference

Comparison of high-performance time scales generated by atomic clocks in laboratories of time and frequency metrology is usually performed by means of the Common View method. Laboratories are equipped with specialized GNSS receivers which measure the difference between a local time scale and a time scale of the selected satellite. Every receiver generates log files in CGGTTS data format to record measured differences. In order to calculate time differences recorded by two receivers, it is necessary to obtain these logs from both receivers and process them. This paper deals with automation and speeding up of these processes.

scholarly journals Application Of The Work Breakdown Structure In Determining Cost Buffers In Construction Schedules

2015 ◽  
Vol 61 (1) ◽  
pp. 147-161 ◽  
Author(s):  
M. Połoński
Keyword(s):  
Construction Projects ◽  
Work Breakdown Structure ◽  
Initial Task ◽  
The Common ◽  
Definition Of ◽  
The Cost ◽  
Construction Schedules

AbstractThe paper presents methods of determining the location of cost buffers and corresponding contingency costs in the CPM schedule based on its work breakdown structure. Application of correctly located cost buffers with appropriately established reserve costs is justified by the common overrunning of scheduled costs in construction projects. Interpolated cost buffers (CB) as separate tasks have been combined with relevant summary tasks by the start–to–start (SS) relationship, whereas the time of their execution has been dynamically connected with the time of accomplishment of particular summary tasks using the “paste connection” option. Besides cost buffers linked with the group of tasks assigned to summary tasks, a definition of the cost buffer for the entire project (PCB) has been proposed, i.e. as one initial task of the entire project. Contingency costs corresponding to these buffers, depending on the data that the planner has at his disposal, can be determined using different methods, but always depend on the costs of all tasks protected by each buffer. The paper presents an exemplary schedule for a facility and the method of determining locations and cost for buffers CB and PCB, as well as their influence on the course of the curve illustrating the budgeted cost of work scheduled (BCWS). The proposed solution has been adjusted and presented with consideration of the possibilities created by the scheduling software MS Project, though its general assumptions may be implemented with application of other similar specialist tools.

Based on Numerical Simulation of High-Performance Parallel Machine Muffler Experimental Calibration

2013 ◽  
Vol 718-720 ◽  
pp. 1645-1650
Author(s):  
Gen Yin Cheng ◽  
Sheng Chen Yu ◽  
Zhi Yong Wei ◽  
Shao Jie Chen ◽  
You Cheng
Keyword(s):  
Numerical Simulation ◽  
Finite Element ◽  
Boundary Element ◽  
Message Passing ◽  
High Performance ◽  
Message Passing Interface ◽  
Parallel Machine ◽  
Simulation Software ◽  
Experimental Calibration ◽  
The Cost

Commonly used commercial simulation software SYSNOISE and ANSYS is run on a single machine (can not directly run on parallel machine) when use the finite element and boundary element to simulate muffler effect, and it will take more than ten days, sometimes even twenty days to work out an exact solution as the large amount of numerical simulation. Use a high performance parallel machine which was built by 32 commercial computers and transform the finite element and boundary element simulation software into a program that can running under the MPI (message passing interface) parallel environment in order to reduce the cost of numerical simulation. The relevant data worked out from the simulation experiment demonstrate that the result effect of the numerical simulation is well. And the computing speed of the high performance parallel machine is 25 ~ 30 times a microcomputer.

Digital monitoring system of equipment for the analysis of fuel and lubricants quality

2021 ◽  
Vol 04 (1) ◽  
pp. 54-54
Author(s):  
V. R. Nigmatullin ◽  
◽  
I. R. Nigmatullin ◽  
R. G. Nigmatullin ◽  
A.M. Migranov ◽  
...  
Keyword(s):  
Monitoring System ◽  
High Performance ◽  
Chemical Elements ◽  
Large Data ◽  
Climatic Conditions ◽  
Point Of View ◽  
Friction Units ◽  
Digital Monitoring ◽  
Wear Products ◽  
The Cost

Currently, to increase the efficiency of industrial production, high-performance and expensive technological equipment is increasingly used, in which the weakest link, from the point of view of efficiency and reliability, is the components and parts of heavily loaded tribo – couplings operating both at significantly different temperatures (conditionally under lighter conditions, the temperature difference can be 100-120 degrees) and climatic conditions (high humidity, the presence of abrasives and other chemical elements in the atmosphere). As the results of the analysis of the frequency of failures of friction units and, accordingly, the cost of their restoration reach 9-20 percent of the cost of all equipment, without taking into account significant losses of income (profit) of the enterprise from downtime. The solution of this problem is based on the study of the wear rate of friction units by the wear products accumulated in working oils, cooling lubricants, and greases. A digital equipment monitoring system (DSMT) has been developed and implemented, which includes dynamic recording of the number of wear products and oil temperature by original modern recording devices, followed by the technology of their processing and use. The system also includes methods for finding the necessary information in large data sets useful and necessary in theoretical and practical terms with a similar technique controlled by a digital monitoring system. The advantages of SMT are the ability to predict the reliability of the equipment; reduce production risks and significantly reduce inefficient costs.

scholarly journals 2 + 1 Highways: Overview and Future Directions

2018 ◽  
Vol 2018 ◽  
pp. 1-13
Author(s):  
Manuel Romana ◽  
Marilo Martin-Gasulla ◽  
Ana T. Moreno
Keyword(s):  
Urban Areas ◽  
High Performance ◽  
White Paper ◽  
Future Directions ◽  
Research Directions ◽  
Traffic Volumes ◽  
Primary Means ◽  
The Common ◽  
Rural Access ◽  
Transportation Research

Most of the rural transportation system is composed of two-lane highways, and many of them serve as the primary means for rural access to urban areas and freeways. In some highways, traffic volumes can be not high enough to justify a four-lane highway but higher than can be served by isolated passing lanes, or can present high number of head-on collisions. In those conditions, 2 + 1 highways are potentially applicable. This type of highway is used to provide high-performance highways as intermediate solution between the common two-lane highway and the freeway. Successful experiences reported in Germany, Sweden, Finland, Poland, or Texas (US) may suggest that they are potentially applicable in other countries. The objective of this white paper is to provide an overview of the past practice in 2 + 1 highways and discuss the research directions and challenges in this field, specially focusing on, but not limited to, operational research in association with the activities of the Subcommittee on Two-Lane Highways (AHB40 2.2) of the Transportation Research Board. The significance of this paper is twofold: (1) it provides wider coverage of past 2 + 1 highways design and evaluation, and (2) it discusses future directions of this field.

scholarly journals Superficial venous insufficiency from the infernal to the endothermal

2014 ◽  
Vol 96 (1) ◽  
pp. 5-10 ◽  
Author(s):  
D Carradice
Keyword(s):  
Gold Standard ◽  
Venous Insufficiency ◽  
Varicose Veins ◽  
Treatment Options ◽  
Resource Limitation ◽  
Skin Damage ◽  
The Common ◽  
Modern Treatment ◽  
The Cost ◽  
Superficial Venous Insufficiency

This review presents the common diseases associated with superficial venous insufficiency of the leg. These include varicose veins, swelling, skin damage and ulceration. The benefits and rationale behind treatment are discussed, followed by the historical advances from ancient mortality and prayer to the modern endovenous revolution. Finally, an overview of modern treatment options will discuss the evidence supporting the gold standard of endothermal ablation and the cost effectiveness of treatment at this time of challenging resource limitation.

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