The Effect ofHelicobacter pyloriEradication on the Levels of Essential Trace Elements

Objective. This study was designed to compare the effect ofHelicobacter pylori(H. pylori) infection treatment on serum zinc, copper, and selenium levels.Patients and Methods. We measured the serum zinc, copper, and selenium levels inH. pylori-positive andH. pylori-negative patients. We also evaluated the serum levels of these trace elements afterH. pylorieradication. These serum copper, zinc, and selenium levels were determined by inductively coupled plasma mass spectrometry.Results. Sixty-threeH. pylori-positive patients and thirtyH. pylori-negative patients were studied. Serum copper, zinc, and selenium levels had no significant difference betweenH. pylori-positive andH. pylori-negative groups. There were 49 patients with successfulH. pylorieradication. The serum selenium levels were lower after successfulH. pylorieradication, but not significantly (P=0.06). There were 14 patients with failedH. pylorieradication. In this failed group, the serum selenium level afterH. pylorieradication therapy was significantly lower than that beforeH. pylorieradication therapy (P<0.05). The serum zinc and copper levels had no significant difference between before and afterH. pylorieradication therapies.Conclusion.H pylorieradication regimen appears to influence the serum selenium concentration (IRB number: KMUH-IRB-20120327).

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Estimation of Trace Elements in Gingival Crevicular Fluid and Serum-Comparative Study in Healthy and Periodontitis

International Journal of Pharmaceutical and Clinical Research ◽

10.25258/ijpcr.v9i5.8599 ◽

2017 ◽

Vol 9 (5) ◽

Author(s):

Bhagavathami Meenaksh ◽

Chitraa R. Chandran ◽

Aravindhan Thathchari Ranganathan ◽

Kavindapadi Venkateswaran Rama ◽

Valarmathy Srinivasan

Keyword(s):

Trace Elements ◽

Inductively Coupled Plasma ◽

Gingival Crevicular Fluid ◽

Serum Zinc ◽

Cross Sectional Study ◽

Serum Copper ◽

Cross Sectional ◽

Inductively Coupled ◽

Significant Difference ◽

Copper Zinc

Background: This study aims to determine and compare the levels of trace elements copper, zinc, selenium and chromium in GCF and serum of patients with periodontitis and healthy individuals. Methods: This cross sectional study includes 24 study subjects recruited from the patients reporting to the Department of Periodontics , Tagore Dental College Chennai. All the selected patients were subjected to a clinical examination done by a single examiner. The estimation of trace elements Copper, Zinc, Selenium and Chromium in GCF and serum is performed using Perkin Elmer optima 5300 Inductively Coupled Plasma Emission Spectrometer (ICPOES). Results: GCF and serum copper levels showed no significant difference in both periodontitis and healthy groups . Selenium levels tend to be the same in both groups. Serum zinc levels are more in periodontitis patients than healthy subjects (p less than 0.01). GCF chromium levels are found to be more in patients with periodontitis than healthy. Conclusions : More research is therefore needed to monitor the role of these trace elements C with an increased sample size to ascertain whether they are associated with a reduced risk of periodontitis.

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Trace elements in patients with aortic valve sclerosis

Therapeutic Advances in Cardiovascular Disease ◽

10.1177/1753944720985985 ◽

2021 ◽

Vol 15 ◽

pp. 175394472098598

Author(s):

Hataw Al-Taesh ◽

Abuzer Çelekli ◽

Murat Sucu ◽

Seyithan Taysi

Keyword(s):

Trace Elements ◽

Aortic Valve ◽

Inductively Coupled Plasma ◽

Healthy Subjects ◽

Serum Zinc ◽

Serum Levels ◽

Control Group ◽

Aortic Valve Sclerosis ◽

Inductively Coupled ◽

Significant Difference

Background: Aortic valve sclerosis (AVSc) is defined as the thickening and calcification of aortic valve cusps, in the absence of obstruction of ventricular outflow. AVSc is linked with a clear imbalance in some trace elements. Aims: The objective of this study was to investigate the relationship between AVSc and serum levels of iron (Fe), zinc (Zn), selenium (Se), and copper (Cu). Additionally, this research aimed to explore the clinical significance of human serum zinc, selenium, copper, and iron concentrations as a potential new biomarker for AVSc patients and to clarify the pathophysiological role in individuals at risk of developing AVSc. Patients and methods: The study included 40 subjects with AVSc (25% male and 75% female) who were compared with a healthy control group with the same gender ratio. AVSc was based on comprehensive echocardiographic assessments. Blood samples were taken and Zn and Cu concentrations were determined through the use of atomic absorption spectroscopy. Se was measured using an inductively coupled plasma mass spectrometry device and Fe was measured using a Beckman Coulter instrument. Results: There was a significant difference in the prevalence of diabetes, blood pressure levels, and body mass index between the patients and the healthy subjects ( p < 0.05). The differences between the serum Fe, Se, and Cu levels of the AVSc patients and the healthy subjects ( p > 0.05) were recorded. The serum Zn of AVSc patients when compared was significantly lower compared with that of the control group ( p < 0.01). Conclusion: Patients with AVSc had an imbalance in some of the trace elements in their blood. The patient group’s valves had higher serum Cu levels and lower serum Se, Zn, and Fe concentrations compared with the healthy group’s valves. In the valve patients as compared, AVSc had a high prevalence of obesity, hypertension, and diabetes.

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Copper and Copper/Zinc Ratio in a Series of Cystic Fibrosis Patients

Nutrients ◽

10.3390/nu12113344 ◽

2020 ◽

Vol 12 (11) ◽

pp. 3344

Author(s):

Marlene Fabiola Escobedo-Monge ◽

Enrique Barrado ◽

Carmen Alonso Vicente ◽

María Antonieta Escobedo-Monge ◽

María Carmen Torres-Hinojal ◽

...

Keyword(s):

Cystic Fibrosis ◽

Zinc Deficiency ◽

Linear Regression Analysis ◽

Bone Conduction ◽

Serum Zinc ◽

Abdominal Ultrasound ◽

Serum Copper ◽

Cross Sectional ◽

Inflammatory Status ◽

Copper Zinc

Cystic fibrosis (CF) patients require a stable and sufficient supply of micronutrients. Since copper is an essential micronutrient for human development, a cross-sectional study was carried out to investigate the serum copper levels, serum copper/zinc (Cu/Zn) ratios, and their relationship with nutritional indicators in a group of CF patients. Anthropometric, biochemical, and dietary measurements, an abdominal ultrasound, and respiratory and pancreatic tests were conducted. Seventeen CF patients were studied (10 females, 59%), 76.5% of whom were ∆F580. Their mean serum copper (113 ± 23 μg/dL) was normal, and there was only one teenager with hypocupremia (6%) and two children with hypercupremia (18%). A significant association between serum copper and zinc levels was discovered. The Cu/Zn ratio was higher than 1.00 for 94% of patients, which is an indicator of an inflammation status. There was no significant correlation between the serum copper concentrations and respiratory and pancreatic function, respiratory colonization, and the results of the abdominal ultrasound. Linear regression analysis showed that serum copper had a positive association with both the Z-score body mass index (BMI) and mean bone conduction speed (BCS). Therefore, since 94% of CF patients had a Cu/Zn ratio > 1.00, this factor must alert us to consider the risk of zinc deficiency and high inflammatory response. The measurement of serum zinc alone does not show one’s zinc status. However, the Cu/Zn ratio may be an indicator of zinc deficiency and the inflammatory status of CF patients.

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A Comparative Study Between Conventional ICP-OES and the Innovative PLS Model-Assisted ICP-OES for the Assay of Trace Elements

Journal of AOAC International ◽

10.1093/jaoacint/qsaa090 ◽

2020 ◽

Author(s):

Adel M Michael ◽

Ahmed A Mohamed ◽

Yousef A Abdelaziz ◽

Nesma M Fahmy

Keyword(s):

Trace Elements ◽

Elemental Analysis ◽

Inductively Coupled Plasma ◽

Optical Emission ◽

Official Method ◽

Icp Oes ◽

Inductively Coupled ◽

Flame Emission ◽

Significant Difference ◽

Student’S T

Abstract Background Inductively coupled plasma is widely used for elemental analysis with the advantage of being eco-friendly since the discharge is free of contaminants. Objective A rapid, novel method was developed for the quantitation of trace elements using inductively coupled plasma with optical emission spectrometry.This method has the advantage of simultaneous calibration compared to the conventional method. Method The assay was carried out for iron, copper, zinc, and molybdenum using the linear regression model partial least-squares. Results The method was optimized and validated as per the International Conference on Harmonization guidelines, showing highly accurate and precise results. The linearity range was 0.25–4 ppm for all trace elements under investigation. The method was applied for the assay of the cited elements in non-chelated and amino acid chelated multi-mineral preparations in the Egyptian market with acceptable mean percent recovery. Conclusions In comparison with the official method by flame emission, statistical analysis showed no significant difference with Student’s t-test and F-values. Highlights Inductively coupled plasma is superior as all of the elements can be measured simultaneously. The method was found to have a high degree of specificity and can be easily applied in routine elemental analysis in laboratories.

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Endoscopic Reflux Esophagitis and Reflux-Related Symptoms after Helicobacter pylori Eradication Therapy: Meta-Analysis

Journal of Clinical Medicine ◽

10.3390/jcm9093007 ◽

2020 ◽

Vol 9 (9) ◽

pp. 3007 ◽

Cited By ~ 1

Author(s):

Mitsushige Sugimoto ◽

Masaki Murata ◽

Hitomi Mizuno ◽

Eri Iwata ◽

Naoyoshi Nagata ◽

...

Keyword(s):

Helicobacter Pylori ◽

Reflux Esophagitis ◽

Reflux Disease ◽

Meta Analysis ◽

Eradication Therapy ◽

Case Group ◽

Inclusion Criteria ◽

Helicobacter Pylori Eradication ◽

Significant Difference ◽

H Pylori

Backgrounds: The etiology of gastroesophageal reflux disease (GERD) including reflux esophagitis and non-erosive reflux disease is multifactorial and a recent meta-analysis showed no association between the development of GERD and Helicobacter pylori eradication in both Western and East-Asian populations. However, the problem remains that various inclusion criteria are used in these studies, which hinders meta-analysis. With a focus on reflux esophagitis with endoscopic mucosal injury, we meta-analysed to evaluate the association between eradication and reflux esophagitis and symptoms using a clearly defined set of inclusion criteria. Methods: We conducted a meta-analysis of studies published up until March 2020, which compared the incidence of reflux esophagitis and symptoms between patients undergoing H. pylori eradication therapy in a randomized placebo-controlled trial (Category A); between patients with successful and failed eradication (Category B); and between patients with successful vs. failed eradication, receipt of placebo, or no-treatment H. pylori-positives (Category C). Results: A total of 27 studies were included. Significant statistical effects were found for development of endoscopic reflux esophagitis [relative risk (RR): 1.46, 95% confidence interval (CI): 1.16–1.84, p = 0.01] or de novo reflux esophagitis (RR: 1.42, 95% CI: 1.01–2.00, p = 0.03) in the case group that received eradication in all studies, especially in Western populations. There was no significant difference in the incidence of symptoms after eradication between patient and control groups, regardless of category, location of population, or baseline disease. Conclusions: Eradication therapy for H pylori increases the risk of reflux esophagitis, irrespective of past history of esophagitis. In contrast, no effect was seen on reflux-related symptoms.

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Serum Trace Elements Profile in Graves’ Disease Patients with or without Orbitopathy in Northeast China

BioMed Research International ◽

10.1155/2018/3029379 ◽

2018 ◽

Vol 2018 ◽

pp. 1-8 ◽

Cited By ~ 1

Author(s):

Yongping Liu ◽

Shanshan Liu ◽

Jinyuan Mao ◽

Sichen Piao ◽

Jing Qin ◽

...

Keyword(s):

Trace Elements ◽

Northeast China ◽

Thyroid Autoimmunity ◽

Serum Copper ◽

High Serum ◽

Graves Disease ◽

Serum Selenium ◽

Specific Antibodies ◽

Euthyroid Status ◽

Serum Trace Elements

Objective. The purpose of the present study was to investigate serum trace elements in Graves’ disease (GD) patients with or without orbitopathy in Northeast China. Methods. Patients with newly diagnosed Graves’ disease (HyGD) (n=66), GD patients with euthyroid status or subclinical thyroidism after treatment (EUGD) (n=55), GO patients with euthyroid status or subclinical thyroidism after treatment (GO) (n=57), and normal controls (NC) (n=66) were enrolled in this study. Serum trace elements were measured with ICP-MS. Results. Serum selenium (Se) levels in EUGD group (median: 7.53 µg/dL), HyGD group (median: 6.76 µg/dL), and GO group (median: 7.40 µg/dL) were significantly lower than those in NC group (median: 9.20 µg/dL, all P<0.01). Serum copper (Cu) levels in GO group (median: 95.93 µg/dL) were significantly lower than those in the NC group (median: 113.59 µg/dL, P=0.015). After being adjusted for multivariables, thyroid-specific antibodies grade was associated with low Se levels. Hyperthyroidism and thyroid-specific antibodies grade were associated with high Cu levels. In addition, orbitopathy was associated with low Cu levels. Conclusions. Thyroid autoimmunity was associated with low Se levels. Hyperthyroidism and thyroid autoimmunity may be associated with relatively high serum Cu levels. Alternatively, ophthalmopathy may be related to low serum Cu levels.

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TO INVESTIGATE THE EFFECT OF THE PASTEURISATION PROCESS ON TRACE ELEMENTS IN DONOR BREAST MILK

Archives of Disease in Childhood ◽

10.1136/archdischild-2016-311535.27 ◽

2016 ◽

Vol 101 (9) ◽

pp. e2.20-e2 ◽

Cited By ~ 2

Author(s):

Nor Mohd Taufek ◽

David Cartwright ◽

Amitha Hewavitharana ◽

Pieter Koorts ◽

Helen McConachy ◽

...

Keyword(s):

Trace Elements ◽

Breast Milk ◽

Inductively Coupled Plasma ◽

Rank Correlation ◽

Formula Milk ◽

Donor Milk ◽

Inductively Coupled ◽

Spearman’S Rank Correlation ◽

Milk Bank ◽

Significant Difference

AimTo investigate the effect of the pasteurisation process on trace elements in donor breast milk.MethodPremature infants often receive donor breast milk when the mother is unable to produce sufficient breast milk. It is widely accepted that donor milk has considerable advantages over formula milk.1 The Royal Brisbane and Women's Hospital (RBWH) has a milk bank that receives milk donated by women which undergoes a pasteurisation process.2 This study investigated the effect of pasteurisation on a range of trace elements in donor milk.A total of 14 participants who donated to the milk bank were recruited in this study. A 2 ml sample was collected pre- and post- pasteurisation, and frozen at −80 °C. Post-natal age of the milk was documented. Inductively-coupled plasma mass-spectrometry was used to analyse the following trace elements – zinc (Zn), copper (Cu), selenium (Se), manganese (Mn), iodine (I), iron (Fe), molybdenum (Mo) and bromine (Br). The study received ethical approval from RBWH and The University of Queensland Ethics Committee.ResultsNo significant difference was found between the levels of any of the trace elements tested pre- and post-pasteurisation. The following p-values were calculated – Zn (0.82), Cu (0.80), Se (0.97), Mn (0.63), I (0.99), Fe (0.05), Mo (0.41), Br (0.59). The following ranges in mcg/L of trace elements were calculated – Zn (365.4–5460.0), Cu (157.6–820.5), Se (10.6–23.7), Mn (0.55–3.24), I (66.4–215.3), Fe (101.5–473.1), Mo (0.20–5.45), Br (704.9–3379.0). Spearman's rank correlation analysis showed significant correlations between post-natal age of milk and trace elements – Zn (ρ=−0.578), Se (ρ=−0.627). Fe (ρ=−0.704), and Mo (ρ=−0.534). No significant correlation was found for Cu, Mn, I, and Br.ConclusionThis study found that the pasteurisation process had minimal effect on trace element levels in donor breast milk. However, it was noted that there was a correlation between post-natal age of donor milk and Zn, Se, Fe and Mo. Further work is needed to establish factors that may influence levels of trace elements in donor milk such as post-natal age.

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Dynamic changes in Antibiotic Resistance Genes and Gut Microbiota after H. Pylori Eradication Therapies

10.21203/rs.3.rs-777700/v1 ◽

2021 ◽

Author(s):

LINGLING WANG ◽

Haobin Yao ◽

Tereasa Tong ◽

KS Lau ◽

Suet Yi Leung ◽

...

Keyword(s):

Antibiotic Resistance ◽

Gut Microbiota ◽

Resistance Genes ◽

Eradication Therapy ◽

Antibiotic Resistance Genes ◽

Short Term ◽

Significant Difference ◽

Treatment Naïve ◽

Stool Samples ◽

H Pylori

Abstract Background: Short-term antibiotics exposure is associated with alterations in microbiota and antibiotic resistance genes (ARGs) in the human gut. While antibiotics are critical in the successful eradication of Helicobacter pylori, the short-term and long-term impacts on the composition and quantity of antibiotics resistance genes after H. pylori eradication is unclear. This study used whole genome shotgun metagenomic of stool samples to characterize the gut microbiota and ARGs, before and after H. pylori eradication therapy. Results: Forty-four H. pylori-infected patients were recruited including 21 treatment naïve patients who received clarithromycin-based triple therapy (CLA group) and 23 patients who failed previous therapies, in which 10 received levofloxacin-based quadruple therapy [LEVO group] and 13 received other combinations [OTHER group] in the current study. Stool samples were collected at baseline (before current treatment), 6-week and 6-month after eradication therapy. At baseline, there was only a slight difference among the three groups on ARGs and gut microbiota. After eradication therapy, there was a transient but significant increase in gut ARGs 6-week post-therapy, among which the LEVO group had the most significant ARGs alteration compared to other two groups. For treatment naïve patients, those with higher ARG richness and ErmF abundance were prone to fail CLA eradication. For gut microbiota, the bacteria richness decreased at 6-week and there was a significant difference in microbiota community among the three groups at 6-week. Conclusions: Our findings demonstrated the dynamic alterations in gut microbiota and ARGs induced by different eradication therapies, which could influence the choices of antibiotics in eradication therapy.

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TRACE METALS IN CANCER PATIENTS AND THEIR CORRELATION WITH RENAL FUNCTION

Journal of Nepal Medical Association ◽

10.31729/jnma.796 ◽

2003 ◽

Vol 42 (146) ◽

pp. 71-73

Author(s):

Shankhar Majhi ◽

B D Toora ◽

S K Sen ◽

K Anantha ◽

N Anantha

Keyword(s):

Trace Elements ◽

Trace Metals ◽

Cancer Patients ◽

Serum Zinc ◽

Serum Copper ◽

The Body ◽

Significant Elevation ◽

Super Oxide Dismutase ◽

Carcinoma Stomach ◽

Cu And Zn

ABSTRACTTwo trace elements, copper (Cu) and zinc (Zn) in serum are elevated in cancer patients since both areconstituents of super oxide dismutase (SOD) as metalloenzyme, the enzyme that scavenge free radicals andactivated oxygen species in the body. The study of 52 cancer patients comprising of 31 carcinoma stomachand 21 breast cancer showed the elevation of both of these trace elements in serum when compared to 32healthy controls and effected the kidney functions as a consequences. Serum Cu and Zn were estimated byAtomic Absorption Spectrometer (AAS), analyzed statistically by student "t" test and marked elevationwas observed in cancer patient compared to healthy controls. Serum Cu and Zn values were 98.13 ± 17.49μg/dl 99..57 ± 13.91 μg /dl respectively in 38 healthy subjects and these trace metals were 156.23 ± 31.02 μg/dl and 171.86 ± 35.15 μg/dl in 52 cancer patients showing significant elevation (P <0.001). These elevatedelements were routed through kidneys and damaged nephrons since both of them are cationic heavy metals.Nephrotoxicity of these trace metals was reflected as elevated level of blood urea and creatinine and werefound to be 27.44 ± 7.72 mg /dl and 0.83 ± 0.30 mg/dl in control groups and 64.49 ± 49 mg /dl and 1.60 ± 0.72mg/dl in cancer patients respectively, a significant elevation (P<0.001) in study group. The elevation ofthese parameters may indicate renal origin.Key Words: Serum copper, serum zinc, super oxide dismutase, carcinoma stomach, carcinoma breastand nephrotoxicity.

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Does copper deficiency predispose children to otitis media with effusion?

International Journal of Otorhinolaryngology and Head and Neck Surgery ◽

10.18203/issn.2454-5929.ijohns20183691 ◽

2018 ◽

Vol 4 (5) ◽

pp. 1147

Author(s):

Maryam Amizadeh ◽

Sareh Molaei Moghbeli ◽

Hamed Reihani-Kermani ◽

Amir Reihani

Keyword(s):

Otitis Media ◽

Otitis Media With Effusion ◽

Graphite Furnace ◽

Serum Levels ◽

Serum Copper ◽

Copper Level ◽

Control Group ◽

Serum Selenium ◽

Cross Sectional ◽

Significant Difference

Background: Both otitis media with effusion (OME) and copper or selenium have been associated to the immune system, hence it could be proposed that cooper and/or selenium deficiency predisposes children to OME through immune cells dysfunction. The aim of this study was to determine the serum levels of Copper and Selenium in an immundeficient-linked disease - OME – in childhood.Methods: This cross-sectional study was conducted between July 2015 and December 2016 including 80 patients age 1 to 8 years old who were divided into two groups: otitis media with effusion (OME) consisted of 40 patients and the control (n=40). Patients who did not recover after three months conservative treatments were included. Serum levels of Copper and Selenium were measured using graphite furnace atomic absorption spectrometry. Results: Statistical analysis showed no significant differences regarding age and sex were noted between groups. Patients with OME had significantly lower serum copper levels than the control group (p=0.007). However, there was no statistically significant difference in serum selenium levels between groups. The analysis also showed a significant positive correlation between serum copper level and age (correlation coefficient=0.348, p=0.028), whereas, there was no correlation between the measured selenium level and age.Conclusions: The results of the present study showed that serum level of copper might play a role on development of otitis media with effusion in childhood.

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