scholarly journals Elevated Plasma Stromal-Cell-Derived Factor-1 Protein Levels Correlate with Severity in Patients with Community-Acquired Pneumonia

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Ping-Kun Tsai ◽  
Ming-Ju Hsieh ◽  
Hsiang-Ling Wang ◽  
Ming-Chih Chou ◽  
Shun-Fa Yang ◽  
...  
Keyword(s):  
Antibiotic Treatment ◽  
Stromal Cell ◽  
Plasma Concentrations ◽  
Biological Marker ◽  
Community Acquired Pneumonia ◽  
Enzyme Linked Immunosorbent Assay ◽  
Elevated Plasma ◽  
Protein Levels ◽  
Before And After ◽  
Stromal Cell Derived Factor

Background.The aim of this study was to investigate differential changes in plasma levels of stromal-cell-derived factor-1 (SDF-1) before and after antibiotic treatment in patients with community-acquired pneumonia (CAP) and observe the association between the severity of CAP and the plasma SDF-1 level.Methods.We gathered blood specimens from 61 adult CAP patients before and after antibiotic treatment and from 60 healthy controls to measure the plasma concentrations of SDF-1 by using an enzyme-linked immunosorbent assay.Results.The plasma SDF-1 concentration was elevated significantly in patients with CAP before receiving treatment compared with the controls and decreased significantly after the patients received treatment. Leukocyte (WBC) and neutrophil counts and C-reactive protein (CRP) levels decreased significantly after antibiotic treatment. Moreover, differences in the plasma concentration of SDF-1 were significantly correlated with PSI, CURB-65, and APACHE II scores (r=0.389,P=0.002, andn=61;r=0.449,P<0.001, andn=61; andr=0.363,P=0.004, andn=61, resp.).Conclusions.An elevated plasma SDF-1 concentration can be used as a biological marker for the early diagnosis of CAP and for the early detection of its severity.

Elevation of Activated Platelet-Dependent Chemokines in Patients With Anti-CD20 Monoclonal Antibody (Rituximab)−Treated Non-Hodgkin's Lymphoma

2007 ◽  
Vol 13 (2) ◽  
pp. 206-212 ◽  
Author(s):  
Shosaku Nomura ◽  
Kazuyoshi Ishii ◽  
Yuka Kamitsuji ◽  
Nobuhiko Uoshima ◽  
Emiko Ishikawa ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
T Cell ◽  
Hodgkin Lymphoma ◽  
Immunosorbent Assay ◽  
Stromal Cell ◽  
Enzyme Linked Immunosorbent Assay ◽  
Non Hodgkin Lymphoma ◽  
Before And After ◽  
Stromal Cell Derived Factor ◽  
Anti Cd20

.ocn.ne.jp. This study measured and compared levels of some chemokines in patients with rituximab-treated non-Hodgkin lymphoma because they may participate in the mechanism of efficacy of rituximab in non-Hodgkin lymphoma patients. Monocytic chemotactant protein-1, RANTES (regulated on activation, normally T-cell expressed and secreted), eotaxin, interleukin-8, neutrophil-activating protein-78, stromal cell-derived factor-1, and growth-regulating oncogene-α in patients with rituximab-treated non-Hodgkin lymphoma were measured by enzyme-linked immunosorbent assay. Levels of RANTES were higher in non-Hodgkin lymphoma patients than in controls. Levels of monocytic chemotactant protein-1, RANTES, and neutrophil-activating protein-78 were significantly elevated before and after chemotherapy with rituximab treatment. However, the level of stromal cell-derived factor-1 did not exhibit a significant change. Before to after chemotherapy without rituximab treatment, all chemokine levels did not exhibit significant changes. These findings suggest that activated platelet-dependent chemokines such as RANTES and neutrophil-activating protein-78 may modulate the efficacy of rituximab in antibody-dependent cellular cytotoxity.

scholarly journals The stromal cell-derived factor-1 α (SDF-1α)/cysteine-X-cysteine chemokine receptor 4 (CXCR4) axis: a possible prognostic indicator of acute ischemic stroke

2019 ◽  
Vol 47 (5) ◽  
pp. 1897-1907
Author(s):  
Xianjun Huang ◽  
Mei Wan ◽  
Qian Yang ◽  
Xianhui Ding ◽  
Zhiming Zhou
Keyword(s):  
Ischemic Stroke ◽  
Acute Ischemic Stroke ◽  
Scale Score ◽  
Chemokine Receptor ◽  
Stromal Cell ◽  
Infarct Volume ◽  
Enzyme Linked Immunosorbent Assay ◽  
Stromal Cell Derived Factor ◽  
Chemokine Receptor 4 ◽  
Cxcr4 Axis

Objective The stromal cell-derived factor-1α/cysteine-X-cysteine chemokine receptor 4 (SDF-1α/CXCR4) axis promotes neuroprotection and angiogenesis in animal studies. Few studies have investigated the potential clinical implications of the SDF-1α/CXCR4 axis in patients with acute ischemic stroke (AIS). We evaluated the prognostic values of the SDF-1α/CXCR4 axis in patients with proximal middle cerebral artery occlusion. Methods Fifty-five patients and 18 age- and sex-matched volunteers were enrolled. Baseline clinical characteristics and risk factors of stroke were recorded. Peripheral whole blood cells were double stained with anti-CD34 and anti-CXCR4 (CD184). CD34+CXCR4+ cells were analyzed by flow cytometry. Plasma SDF-1α levels were measured by enzyme-linked immunosorbent assay. Results In the AIS group, plasma SDF-1α levels and the number of circulating CD34+CXCR4+ cells were significantly higher than those in controls. Day 1 SDF-1α levels were negatively correlated with infarct volume (r = −0.521) and the initial National Institutes of Health Stroke Scale score (r = −0.489). SDF-1α levels (day 1: r = −0.514; day 3: r = −0.275; day 7: r = −0.375) and circulating CD34+CXCR4+ cells (day 7: r = −0.282) were inversely associated with the 90-day modified Rankin Scale score. Conclusion The SDF-1α/CXCR4 axis has potential applications for predicting the clinical outcome of AIS.

scholarly journals Elevated Plasma Soluble PD-L1 Levels in Out-of-Hospital Cardiac Arrest Patients

2021 ◽  
Vol 10 (18) ◽  
pp. 4188
Author(s):  
Miho Sumiyoshi ◽  
Eiji Kawamoto ◽  
Yuki Nakamori ◽  
Ryo Esumi ◽  
Kaoru Ikejiri ◽  
...  
Keyword(s):  
Cardiac Arrest ◽  
Ischemia Reperfusion ◽  
Plasma Concentrations ◽  
Spontaneous Circulation ◽  
University Hospital ◽  
Soluble Form ◽  
Enzyme Linked Immunosorbent Assay ◽  
Immune Functions ◽  
Protein Levels ◽  
Hospital Cardiac Arrest

Background: A deregulated immune system has been implicated in the pathogenesis of post-cardiac arrest syndrome (PCAS). A soluble form of programmed cell death-1 (PD-1) ligand (sPD-L1) has been found at increased levels in cancer and sustained inflammation, thereby deregulating immune functions. Here, we aim to study the possible involvement of sPD-L1 in PCAS. Methods: Thirty out-of-hospital cardiac arrest (OHCA) patients consecutively admitted to the ER of Mie University Hospital were prospectively enrolled. Plasma concentrations of sPD-L1 were measured by an enzyme-linked immunosorbent assay in blood samples of all 30 OHCA patients obtained during cardiopulmonary resuscitation (CPR). In 13 patients who achieved return-of-spontaneous-circulation (ROSC), sPD-L1 levels were also measured daily in the ICU. Results: The plasma concentrations of sPD-L1 in OHCA were significantly increased; in fact, to levels as high as those observed in sepsis. sPD-L1 levels during CPR correlated with reduced peripheral lymphocyte counts and increased C-reactive protein levels. Of 13 ROSC patients, 7 cases survived in the ICU for more than 4 days. A longitudinal analysis of sPD-L1 levels in the 7 ROSC cases revealed that sPD-L1 levels occurred in parallel with organ failure. Conclusions: This study suggests that ischemia- reperfusion during CPR may aberrantly activate immune and endothelial cells to release sPD-L1 into circulation, which may play a role in the pathogenesis of immune exhaustion and organ failures associated with PCAS.

scholarly journals Comparative effectiveness of two methods for inducing osteoarthritis in a novel animal model, the Diannan small-ear pig1

2020 ◽  
Author(s):  
Di Jia ◽  
Yinghong He ◽  
Guofeng Cai ◽  
Jiali Zheng ◽  
Yuye Yang ◽  
...  
Keyword(s):  
Stromal Cell ◽  
Collagen Type ◽  
The Other ◽  
Cartilage Loss ◽  
Type Ii ◽  
Collagen Type Ii ◽  
Matrix Metalloproteinase 3 ◽  
Protein Levels ◽  
Stromal Cell Derived Factor ◽  
Chemical Groups

Abstract Background: Varieties of animals were used to study osteoarthritis pathogenesis. The Diannan small-ear pig, which is native to Yunnan, China, is thought to have an articular anatomy similar to that of humans and is more likely to be a source of pathological tissues than other animals. The aim of this study was determine whether this animal can serve as a more effective osteoarthritis model.[A1] Methods: Twenty-seven adult pigs were randomly divided into three groups and underwent the Hulth procedure, papain articular injection [A2] , and conventional breeding. After 4, 8, and 12 weeks, cartilage tissues from knee joint were extracted for general and histological observation, immunofluorescence, and biochemical analysis. [A3] Synovium was taken out for stromal cell-derived factor-1 analysis. Results: Histopathological observation showed obvious cartilage loss in two experimental groups, this cartilage loss was more severe in the chemical groups. Synovial stromal cell-derived factor1 levels increased over time in all groups. mRNA and protein levels of matrix metalloproteinase-3 were much higher in the chemical groups than in the other groups, whereas levels of collagen type II[A4] and aggrecan were significantly lower in the chemical groups than in the other groups. Immunofluorescence assays of collagen type II[A5] revealed an apparent reduction in this marker in the chemical groups compared with the other groups. Conclusions: These results indicated that the Diannan small-ear pig can be used as an effective osteoarthritis model. In addition, it is much more convenient and much faster to induce osteoarthritis by intra-articular injection of papain, which is a method worthy of being promoted.

Elevated Plasma Stromal Cell-Derived Factor 1 Protein Level in the Progression of HIV Type 1 Infection/AIDS

2001 ◽  
Vol 17 (7) ◽  
pp. 587-595 ◽  
Author(s):  
Masaya Ikegawa ◽  
Jingli Yuan ◽  
Kazuko Matsumoto ◽  
Steve Herrmann ◽  
Aikichi Iwamoto ◽  
...  
Keyword(s):  
Protein Level ◽  
Stromal Cell ◽  
Elevated Plasma ◽  
Hiv Type 1 ◽  
Stromal Cell Derived Factor

PROLACTIN AND THE PITUITARY-GONADAL AXIS IN MALE URAEMIC PATIENTS ON REGULAR DIALYSIS

1976 ◽  
Vol 82 (1) ◽  
pp. 29-38 ◽  
Author(s):  
C. Hagen ◽  
K. Ølgaard ◽  
A. S. McNeilly ◽  
R. Fisher
Keyword(s):  
Growth Hormone ◽  
Follicle Stimulating Hormone ◽  
Plasma Concentrations ◽  
Sex Hormone Binding Globulin ◽  
Elevated Plasma ◽  
Hormone Binding ◽  
Hormone Levels ◽  
Significant Difference ◽  
Before And After ◽  
Male Patients

ABSTRACT In 21 consecutive adult male patients with chronic renal failure on regular haemo- or peritoneal dialysis, the plasma levels of prolactin (hPr), growth hormone (HGH), thyrotrophin (TSH), luteinizing hormone (LH), follicle stimulating hormone (FSH), testosterone (T) and sex hormone binding globulin (SHBG) were measured. Elevated levels of hPr were found in 16 of the patients and could not only be explained by the medicamentation. All the patients studied showed an inverse ratio of LH to FSH with higher levels of FSH than LH and 15 of the 21 patients had elevated plasma concentrations of FSH, while only 4 had elevated LH. No significant difference in any of the hormone levels could be demonstrated before and after dialysis, and no significant correlation between the hormone levels and the time of dialysis, the type of dialysis or the age of the patient was found. However, 8 of the 21 patients showed higher levels of HGH before than after dialysis. Impotency was found in 11 of the patients, but was not related to abnormal levels of hPr, LH, FSH, T or SHBG.

Effects of physical training and lean body mass of plasma amino acids in man

1978 ◽  
Vol 45 (2) ◽  
pp. 177-181 ◽  
Author(s):  
G. Holm ◽  
L. Sullivan ◽  
R. Jagenburg ◽  
P. Bjorntorp
Keyword(s):  
Amino Acids ◽  
Lean Body Mass ◽  
Body Mass ◽  
Physical Training ◽  
Plasma Concentrations ◽  
Insulin Level ◽  
Elevated Plasma ◽  
Isoleucine Leucine ◽  
Before And After ◽  
Obese Subjects

Postabsorptive plasma amino acid and insulin concentrations were determined in subjects with hyperplastic obesity and in nonobese controls before and after a 6-wk period of physical training. After the training period the plasma concentrations of insulin and leucine decreased and the concentration of alanine increased in the obese subjects. No changes were noticed in the controls. The obese subjects had elevated plasma levels of valine, isoleucine, leucine, tyrosine, and phenylalanine before as well as after physical training. The concentrations of these amino acids were correlated to the plasma insulin level and to lean body mass before but only to lean body mass after physical training. It is suggested that the lean body mass, whick is higher in hyperplastic obesity, contributes to the elevated concentrations of amino acids, and it is unlikely that the insulin decreases in the obese subjects after physical training is mediated through an effect of amino acids on insulin secretion.

Elevated Plasma Stromal Cell-derived Factor 1 Protein and its Gene Polymorphism in Patients With Pelvic Inflammatory Disease

2009 ◽  
Vol 16 (6) ◽  
pp. 610-617 ◽  
Author(s):  
Hsiu-Ting Tsai ◽  
Yi-Torng Tee ◽  
Yi-Hsien Hsieh ◽  
Hui-Ling Chiou ◽  
Chiao-Wen Lin ◽  
...  
Keyword(s):  
Gene Polymorphism ◽  
Pelvic Inflammatory Disease ◽  
Inflammatory Disease ◽  
Stromal Cell ◽  
Elevated Plasma ◽  
Its Gene ◽  
Stromal Cell Derived Factor

Hyperhomocysteinemia and cardiovascular risk in postmenopausal women: the role of folate supplementation

2007 ◽  
Vol 45 (2) ◽  
Author(s):  
Paola Villa ◽  
Rosanna Suriano ◽  
Barbara Costantini ◽  
Francesca Macrì ◽  
Luigi Ricciardi ◽  
...  
Keyword(s):  
Endothelial Function ◽  
Postmenopausal Women ◽  
Plasma Concentrations ◽  
High Plasma ◽  
Folic Acid Supplementation ◽  
Endothelial Damage ◽  
Elevated Plasma ◽  
Folate Supplementation ◽  
Before And After ◽  
Artery Flow

AbstractIn the postmenopausal period, cardiovascular diseases are a frequent chronic condition leading to high risk of myocardial infarction and death. Recently hyperhomocysteinemia and even mildly elevated plasma concentrations of homocysteine have been recognized as independent risk factors for vascular damage predisposing to arteriosclerosis. Elevated plasma levels of homocysteine induce vascular endothelial damage and are frequently associated with low folate levels.In this review we evaluate literature data on some aspects related to menopause and homocysteine metabolism. In particular, we show the effect of folic acid supplementation on homocysteine concentrations and on homocysteine-related thiols, such as cysteine and cysteine-glycine, as well as the relationship with glucose, insulin, and lipidic metabolism in postmenopausal women. We also analyze the influence of folate supplementation on endothelial function, by brachial artery flow-mediated dilatation (endothelium-dependent) and nitroglycerine-induced dilatation (endothelium-independent) before and after a methionine load.Folate administration in postmenopausal women is able to reduce high plasma homocysteine levels and to modify impaired endothelial function induced by hyperhomocysteinemia.Clin Chem Lab Med 2007;45:130–5.

scholarly journals Interleukin (IL)-1β Regulation of IL-1β and IL-1 Receptor Antagonist Expression in Cultured Human Endometrial Stromal Cells1

2001 ◽  
Vol 86 (3) ◽  
pp. 1387-1393 ◽  
Author(s):  
Hong-Yuan Huang ◽  
Yan Wen ◽  
Jan S. Kruessel ◽  
Francisco Raga ◽  
Yung-Kuei Soong ◽  
...  
Keyword(s):  
Receptor Antagonist ◽  
Stromal Cells ◽  
Stromal Cell ◽  
Internal Standard ◽  
Enzyme Linked Immunosorbent Assay ◽  
Dependent Manner ◽  
Cellular Interactions ◽  
Intracellular Protein ◽  
Protein Levels ◽  
Quantitative Ratio

The interleukin (IL)-1 system is a major regulator of local cellular interactions during embryonic implantation. Because IL-1β and IL receptor antagonist (IL-1ra) are both expressed in human endometrium, we hypothesized that an appropriate ratio of IL-1β to IL-1ra might favor the process of embryo implantation. Therefore, we investigated IL-1 regulation of the quantitative ratio of IL-1β/IL-1ra messenger RNA (mRNA) expression in human endometrial stromal cells using quantitative competitive PCR, as well as intracellular protein expression after stromal cell solubilization. Confluent stromal cell cultures were stimulated with human IL-1β (0–1000 IU/mL) for 24 h. After 24 h, total RNA was extracted, reverse transcribed, and coamplified by PCR with a defined amount of internal standard. The quantitative ratio was determined by the density of target to the internal standard. After culture with IL-1β for 24 and 48 h, stromal cells were solubilized, and the intracellular protein levels of IL-1β and IL-1ra were measured by enzyme-linked immunosorbent assay. The IL-1β and IL-1ra mRNA were both up-regulated, and IL-1R tI mRNA was down-regulated, by IL-1β in a dose-dependent manner. The quantitative ratio of IL-1β to IL-1ra mRNA was constant with the presence of increasing concentrations of IL-1β (1–1000 IU/mL). IL-1β and IL-1ra protein was not detected in conditioned media of cultures before addition of IL-1β. IL-1β and IL-1ra protein levels increased with increasing amounts of IL-1β after solubilization of stromal cells. The IL-1β was detectable after 12 h of culture, in comparison with IL-1ra, which was detectable after 24 h of IL-1β stimulation. These results suggest that IL-1 may play a crucial role in embryo-maternal interaction by regulating stromal cell expression of IL-1β and IL-1ra, resulting in an appropriate ratio during the process of embryonic implantation.

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