scholarly journals Moringa oleiferaSupplemented Diets Prevented Nickel-Induced Nephrotoxicity in Wistar Rats

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
O. S. Adeyemi ◽  
T. C. Elebiyo
Keyword(s):  
Wistar Rats ◽  
Moringa Oleifera ◽  
Weight Ratio ◽  
Normal Diet ◽  
Oral Exposure ◽  
Renal Tubules ◽  
Nickel Sulphate ◽  
Significant Elevation ◽  
Body Weight Ratio ◽  
Male Wistar Rats

Background. TheMoringa oleiferaplant has been implicated for several therapeutic potentials.Objective. To evaluate whether addition ofM. oleiferato diet has protective effect against nickel-induced nephrotoxicity in rats.Methodology. Male Wistar rats were assigned into six groups of five. The rats were given oral exposure to 20 mg/kg nickel sulphate (NiSO4) in normal saline and sustained on either normal diet or diets supplemented withMoringa oleiferaat different concentrations for 21 days. 24 hours after cessation of treatments, all animals were sacrificed under slight anesthesia. The blood and kidney samples were collected for biochemical and histopathology analyses, respectively.Results. NiSO4exposure reduced the kidney-to-body weight ratio in rats and caused significant elevation in the levels of plasma creatinine, urea, and potassium. Also, the plasma level of sodium was decreased by NiSO4exposure. However, addition ofM. oleiferato diets averted the nickel-induced alteration to the level of creatinine and urea. The histopathology revealed damaged renal tubules and glomerular walls caused by NiSO4exposure. In contrast, the damages were ameliorated by theM. oleiferasupplemented diets.Conclusion. The addition ofM. oleiferato diet afforded significant protection against nickel-induced nephrotoxicity.

scholarly journals Melatonin effects on pulmonary tissue in the experimental model of Hepatopulmonary Syndrome

2019 ◽  
Vol 45 (3) ◽  
Author(s):  
Adriane Dal Bosco ◽  
Filipe Boeira Schedler ◽  
Josieli Raskopf Colares ◽  
Elisângela Gonçalves Schemitt ◽  
Renata Minuzzo Hartmann ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Weight Ratio ◽  
Hepatopulmonary Syndrome ◽  
Antioxidant Effect ◽  
Biliary Duct ◽  
Body Weight Ratio ◽  
Pulmonary Tissue ◽  
Male Wistar Rats ◽  
Hepatic Histology ◽  
And Oxidative Stress

ABSTRACT Objective: To evaluate the pulmonary alterations of animals with Hepatopulmonary Syndrome (HPS) submitted to Biliary Duct Ligature (BDL), as well as the antioxidant effect of Melatonin (MEL). Methods: Sixteen male Wistar rats, divided into four Sham groups: BDL group, Sham + MEL group and BDL + MEL. The pulmonary and hepatic histology, lipoperoxidation and antioxidant activity of lung tissue, alveolar-arterial O2 difference and lung / body weight ratio (%) were evaluated. Results: When comparing the groups, could be observed an increase of vasodilation and pulmonary fibrosis in the BDL group and the reduction of this in relation to the BDL + MEL group. It was also observed significant changes in the activity of catalase, ApCO2, ApO2 in the LBD group when compared to the other groups. Conclusion: The use of MEL has been shown to be effective in reducing vasodilation, fibrosis levels and oxidative stress as well as gas exchange in an experimental HPS model.

scholarly journals Lipoic Acid Exacerbates Oxidative Stress and Lipid Accumulation in the Liver of Wistar Rats Fed a Hypercaloric Choline-Deficient Diet

Nutrients ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 1999
Author(s):  
Lidia V. Kravchenko ◽  
Ilya V. Aksenov ◽  
Nikolay S. Nikitin ◽  
Galina V. Guseva ◽  
Ludmila I. Avrenyeva ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Body Weight ◽  
Liver Disease ◽  
Lipoic Acid ◽  
Lipid Accumulation ◽  
Wistar Rats ◽  
Weight Ratio ◽  
Body Weight Ratio ◽  
Deficient Diet ◽  
Male Wistar Rats

Non-alcoholic fatty liver disease (NAFLD) is currently estimated as the most prevalent chronic liver disease in all age groups. An increasing body of evidence obtained in experimental and clinical data indicates that oxidative stress is the most important pathogenic factor in the development of NAFLD. The study aimed to investigate the impact of α-lipoic acid (LA), widely used as an antioxidant, on the effects of a hypercaloric choline-deficient diet. Male Wistar rats were divided into three groups: control diet (C); hypercaloric choline-deficient diet (HCCD), and hypercaloric choline-deficient diet with α-lipoic acid (HCCD+LA). Supplementation of HCCD with LA for eight weeks led to a decrease in visceral adipose tissue/body weight ratio, the activity of liver glutathione peroxidase and paraoxonase-1, plasma, and liver total antioxidant activity, as well as an increase in liver/body weight ratio, liver total lipid and triglyceride content, and liver transaminase activities compared to the HCCD group without LA. In conclusion, our study shows that α-lipoic acid detains obesity development but exacerbates the severity of diet-induced oxidative stress and lipid accumulation in the liver of male Wistar rats fed a hypercaloric choline-deficient diet.

Tissue Deposition of Zinc from a Zinc Chelate and from Inorganic Zinc in Rats

1994 ◽  
Vol 1994 ◽  
pp. 171-171 ◽  
Author(s):  
Ronan Power ◽  
Kevin Cashman ◽  
Albert Flynn
Keyword(s):  
Amino Acids ◽  
Tissue Distribution ◽  
Wistar Rats ◽  
Gel Filtration ◽  
Normal Diet ◽  
Farm Animals ◽  
Trace Minerals ◽  
Inorganic Salts ◽  
Male Wistar Rats ◽  
Differential Tissue

Some reports have suggested differential tissue deposition of dietary trace minerals such as Zinc (Zn) when supplied to farm animals either chelated to amino acids or as inorganic salts. To test this hypothesis, an experiment was conducted to determine the ultimate tissue distribution of Zinc in rats fed either a radioactively-labeled 65Zn-chelate or 65ZnSO4. The 65Zn-chelate was prepared by heating a solution of 65ZnSO4 and an equimolar mixture of glycine and methionine for 5 minutes at 90°C. The resulting chelate was then separated from unincorporated 65ZnSO4 by gel filtration chromatography. Ten 25-d old male wistar rats (mean weight 34.5 g) were randomized by weight into two groups (n = 5/group), fasted for 18 hours and given 0.4 ml (8 μg Zn, 1 μCi65Zn) of one or other labelled solution by gavage. Four hours later, animals were returned to their normal diet for the duration of the experiment. The 65Zn activity of the animals was determined two hours after administration and daily thereafter for 7 days.

Attenuation of Lead-Induced Oxidative Stress in Rat Brain, Liver, Kidney and Blood of Male Wistar Rats by Moringa Oleifera Seed Powder

2014 ◽  
Vol 33 (4) ◽  
pp. 323-337 ◽  
Author(s):  
Manoj Kumar Velaga ◽  
Lucius K. Daughtry ◽  
Angelica C. Jones ◽  
Prabhakara Rao Yallapragada ◽  
Sharada Rajanna ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Rat Brain ◽  
Wistar Rats ◽  
Moringa Oleifera ◽  
Male Wistar Rats ◽  
Moringa Oleifera Seed

Dietary linoleic acid and salt-induced hypertension

1981 ◽  
Vol 59 (8) ◽  
pp. 872-875 ◽  
Author(s):  
Murray C. Macdonald ◽  
Robert L. Kline ◽  
Gordon J. Mogenson
Keyword(s):  
Blood Pressure ◽  
Linoleic Acid ◽  
Systolic Blood Pressure ◽  
Wistar Rats ◽  
Sodium Content ◽  
Significant Elevation ◽  
Dietary Linoleic Acid ◽  
Low Level ◽  
Induced Hypertension ◽  
Male Wistar Rats

Male Wistar rats chronically fed a low level (0.41%) of linoleic acid (LA) in the diet as supplied by 5% olive oil developed a significant elevation of systolic blood pressure as compared with rats fed either a medium (4.2%) or high (9.4%) level of dietary LA. Chronic excess intake of NaCl (3.75% in the diet) was associated with a significant elevation of blood pressure on all three diets but a low level of LA in the diet exaggerated the salt-induced hypertension. The results suggest that inadequate dietary LA may result in an increase in systolic blood pressure regardless of the sodium content of the diet.

Studies on the toxicological effect of the aqueous extract of the fresh, dried and boiled berries of Solanum aculeastrum Dunal in male Wistar rats

2009 ◽  
Vol 28 (12) ◽  
pp. 765-775 ◽  
Author(s):  
OM Aboyade ◽  
MT Yakubu ◽  
DS Grierson ◽  
AJ Afolayan
Keyword(s):  
Body Weight ◽  
Aqueous Extract ◽  
Wistar Rats ◽  
Haemoglobin Concentration ◽  
Weight Ratio ◽  
The Body ◽  
Corpuscular Haemoglobin ◽  
Toxicological Effect ◽  
Male Wistar Rats ◽  
Mean Corpuscular Haemoglobin

The toxicological effect of the aqueous extract of fresh, dried and boiled berries of Solanum aculeastrum Dunal at 1, 10 and 25 mg/kg body weight was investigated in male Wistar rats for 28 days. The parameters used were the body weight of the animals and absolute weights of the organs, haematological parameters, renal and liver functional endpoints. The animals gained appreciable weight and showed no signs of clinical toxicity. The dried (DB), boiled dried (BDB), fresh (FB) and boiled fresh berry (BFB) extracts reduced (p < .05) the heart-, liver-and spleen-body weight ratio of the animals whereas that of the lung was not altered. The kidney and testes-body weight ratios were specifically altered by the different extract. All these were not accompanied by any histomorphological changes. The extracts did not alter (p > .05) the levels of RBC, Hb, PCV and albumin of the animals. The platelets were decreased by the DB and FB whereas BFB increased this parameter. The FB and BFB at all the doses also reduced the mean corpuscular haemoglobin (MCH) and mean corpuscular haemoglobin concentration (MCHC) of the animals. With the exception of the FB where the creatinine and chloride levels decreased, other extracts did not alter the level of these kidney parameters. Only FB increased the levels of uric acid and urea. All the extract decreased the serum alanine aminotransferase (ALT) of the animal. The levels of total protein, globulin, total and conjugated bilirubin were not altered by DB and BDB whereas these indices were increased by FB and BFB. The DB and BDB increased the serum alkaline phosphatase (ALP) activity whereas FB decreased the activity of the enzyme. In contrast, DB and BDB decreased the serum aspartate aminotransferase (AST) activity of the animals whereas FB and BFB increased the activity of the enzyme. The FB and BFB also increased the levels of potassium, magnesium and phosphorus of the animals. Overall, the alterations in the biochemical parameters by the various extracts of S. aculeastrum berries at these doses indicated that the normal functioning of these organs may be adversely affected. However, drying and boiling might reduce the toxic effect of the berries.

scholarly journals Hematoprotective and Gastroprotective Effects of Cnidoscolus aconitifolius (Linn)-Supplemented Diet in Male Wistar Rats

2020 ◽  
pp. 107-118
Author(s):  
Samuel A. Onasanwo ◽  
Olusegun G. Adebayo ◽  
Omeje U. Nicodemus ◽  
Abayomi M. Ajayi ◽  
A. Wadioni ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Structural Integrity ◽  
Progressive Increase ◽  
Diet Group ◽  
Normal Diet ◽  
Hematological Indices ◽  
Cnidoscolus Aconitifolius ◽  
Male Wistar Rats ◽  
Anti Oxidant ◽  
Plasma Malondialdehyde

Aims: Cnidoscolus aconitifolius (CA) is a plant known to possess very high phenolic compound. The study evaluated hematological indices, anti-oxidative properties and gastroprotective potentials of CA leaf-supplemented diet. Methodology: Male Wistar rats weighing between 80-100 g were divided into 5 groups of n=18. Group 1 animals were fed with normal diet and groups 2-5 were fed with diets supplemented with 1%, 2.5%, 5% and 10% of C. aconitifolius powdered leaves continuously for 4, 8 and 12 weeks. Hematological indices were examined in the whole blood plasma Malondialdehyde (MDA), Glutathione (GSH), Catalase (CAT) were investigated, and changes in stomach architecture were also examined via histology. Results: There was a progressive increase in weight gain at different weeks of feeding with Cnidoscolus aconitifolius-supplemented diet (CAD). Malondialdehyde level decreased (p<0.05) significantly in 1% CAD at 8 weeks of feeding when compared to normal diet group. Glutathione at the 8th and 12th weeks of feeding with 1% CAD and 10% CAD significantly (p<0.05) decreased when compared to the group fed normal diet. Catalase activity increased (p<0.05) significantly in 1%, 2.5% and 5% CAD groups when compared to the group fed normal diet at 4 weeks of feeding. There was increase in haematological indices across all the groups during the weeks of feeding. The microscopic examination of the stomach epithelial layer reveals improvement with no serious damage across all the groups. Conclusion: Cnidoscolus aconitifolius-supplemented diet moderately improves blood anti-oxidant against oxidative stress and the hematological indices with time. The diet also maintains the structural integrity of the stomach wall without causing any damage.

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