scholarly journals Metabolomics Analysis of Seminal Plasma in Infertile Males with Kidney-Yang Deficiency: A Preliminary Study

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Xiang Chen ◽  
Chao Hu ◽  
Jican Dai ◽  
Lei Chen
Keyword(s):  
Male Infertility ◽  
Seminal Plasma ◽  
Metabolic Pathways ◽  
Tricarboxylic Acid Cycle ◽  
Principal Component ◽  
Metabolite Identification ◽  
Deficiency Syndrome ◽  
Sphingolipid Metabolism ◽  
Kidney Yang Deficiency ◽  
Metabolomics Analysis

Traditional Chinese medicine (TCM) is an important treatment for male infertility, and its application to therapy is dependent on differentiation of TCM syndromes. This study aims to investigate the changes in metabolites and metabolic pathways in infertile males with Kidney-Yang Deficiency syndrome (KYDS) via metabolomics approaches. Seminal plasma samples were collected from 18 infertile males with KYDS and 18 fertile males. Liquid chromatography and mass spectrometry were used to characterize metabolomics profiles. Principal component analysis (PCA), partial least squares-discriminate analysis (PLS-DA), and pathway analysis were used for pattern recognition and metabolite identification. PCA and PLS-DA results differentiated the two groups of patients. Forty-one discriminating metabolites (18 in positive mode and 23 in negative mode) were identified. Seven metabolites were related to five potential metabolic pathways associated with biosynthesis and metabolism of aromatic amino acids, tricarboxylic acid cycle, and sphingolipid metabolism. The changes in metabolic pathways may play an important role in the origin of KYDS-associated male infertility. Metabolomics analysis of seminal plasma may be used to differentiate TCM syndromes of infertile males, but further research must be conducted.

scholarly journals Plasma Metabolomics Analysis Based on GC-MS in Infertile Males with Kidney-Yang Deficiency Syndrome

2017 ◽  
Vol 2017 ◽  
pp. 1-11 ◽  
Author(s):  
Piao Zheng ◽  
Yun Wang ◽  
Hongmei Lu ◽  
Xinyi Zhou ◽  
Tao Tang ◽  
...  
Keyword(s):  
Chinese Medicine ◽  
Male Infertility ◽  
Metabolic Pathways ◽  
Characteristic Curve ◽  
Deficiency Syndrome ◽  
Gas Chromatography Mass Spectrometry ◽  
Healthy Controls ◽  
Chinese Medicine Syndrome ◽  
Kidney Yang Deficiency ◽  
Potential Biomarkers

Introduction. Chinese medicine syndrome diagnosis is the key requisite in the treatment of male infertility with traditional Chinese medicine (TCM). Kidney-Yang deficiency syndrome (KYDS) is the critical Chinese medicine syndrome of male infertility. To explore the modernized mechanisms of KYDS in male infertility, this study aims to investigate the metabolomics of males with KYDS. Methods. The gas chromatography-mass spectrometry method was applied to analyze the plasma samples of 67 infertile males with KYDS compared with 55 age-matched healthy controls. The chemometric methods including principal component and partial least squares-discriminate analyses were employed to identify the potential biochemical patterns. With the help of the variable importance for the projection and receiver operating characteristic curve analyses, the potential biomarkers were extracted to define the clinical utility. Simultaneously the high-quality KEGG metabolic pathways database was used to identify the related metabolic pathways. Results. The metabolomics profiles of infertile males with KYDS including 10 potential biomarkers and six metabolic pathways were identified. They precisely distinguished infertile males with KYDS from healthy controls. Conclusions. These potential biomarkers and pathways suggest the substantial basis of infertile males with KYDS. The metabolomics profiles highlight the modernized mechanisms of infertile males with KYDS.

scholarly journals The Difference of Chemical Components and Biological Activities of the Crude Products and the Salt-Processed Product from Semen Cuscutae

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Song Yang ◽  
Hefang Xu ◽  
Baosheng Zhao ◽  
Shasha Li ◽  
Tingting Li ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Biological Activities ◽  
Biological Effects ◽  
Principal Component ◽  
Deficiency Syndrome ◽  
Antioxidant Effect ◽  
Chemical Components ◽  
Crude Product ◽  
Kidney Yang Deficiency ◽  
The Difference

Semen Cuscutae is a well-known Chinese medicine which has been used to nourish kidney in China for thousands of years. The crude product of semen Cuscutae (CP) and its salt-processed product (SPP) are separately used in clinic for their different effects. The study was designed to investigate the influence of processing from semen Cuscutae on chemical components and biological effects. The principal component analysis and quantitative analysis were used to study the differences of the chemical components. The effects of nourishing kidney were detected to compare the differences between the CP and SPP. The PCA results showed that the obvious separation was achieved in the CP and SPP samples. The results of quantitative analysis showed that quercetin and total flavonoids had significantly increased after salt processing while hyperoside had decreased. The comparison of CP and SPP on biological activities showed that both of them could ameliorate the kidney-yang deficiency syndrome by restoring the level of sex hormone, improving the immune function and antioxidant effect. However, SPP was better in increasing the level of T and the viscera weight of testicle and epididymis, improving the antioxidant effect. The results suggested that salt processing changed its chemical profile, which in turn enhanced its biological activities.

scholarly journals Integrated Systems Pharmacology, Urinary Metabonomics, and Quantitative Real-Time PCR Analysis to Uncover Targets and Metabolic Pathways of the You-Gui Pill in Treating Kidney-Yang Deficiency Syndrome

2019 ◽  
Vol 20 (15) ◽  
pp. 3655 ◽  
Author(s):  
Ruiqun Chen ◽  
Jia Wang ◽  
Runhua Zhan ◽  
Lei Zhang ◽  
Xiufeng Wang
Keyword(s):  
Glutamine Synthetase ◽  
Metabolic Pathways ◽  
Acid Metabolism ◽  
Deficiency Syndrome ◽  
Pcr Analysis ◽  
Systems Pharmacology ◽  
Quantitative Real Time Pcr ◽  
Target Proteins ◽  
Kidney Yang Deficiency ◽  
Hydroxyacyl Coa Dehydrogenase

Kidney-yang deficiency syndrome (KYDS) is a metabolic disease caused by a neuro-endocrine disorder. The You-gui pill (YGP) is a classic traditional Chinese medicine (TCM) formula for the treatment of KYDS and has been widely used to warm and recuperate KYDS clinically for hundreds of years in China. However, it is unknown whetherthe corresponding targets and metabolic pathways can also be found via using metabonomics based on one platform (e.g., 1H NMR) to study different biological samples of KYDS. At the same time, relevant reports on further molecular verification (e.g., RT-qPCR analysis) of these targets associated with biomarkers and metabolic pathways have not yet, to our knowledge, been seen in KYDS’s research. In the present study, a comprehensive strategy integrating systems pharmacology and 1H NMR-based urinary metabonomics analysis was proposed to identify the target proteins and metabolic pathways that YGP acts on KYDS. Thereafter, further validation of target proteins in kidney tissue was performed through quantitative real-time PCR analysis (RT-qPCR). Furthermore, biochemical parameters and histopathological analysis were studied. As a result, seven target proteins (L-serine dehydratase; phosphoenolpyruvate carboxykinase; spermidine synthase; tyrosyl-tRNA synthetase, glutamine synthetase; 3-hydroxyacyl-CoA dehydrogenase; glycine amidinotransferase) in YGP were discovered to play a therapeutic role in KYDS via affecting eight metabolic pathways (glycine, serine and threonine metabolism; butanoate metabolism; TCA cycle, etc.). Importantly, three target proteins (i.e., 3-hydroxyacyl-CoA dehydrogenase; glutamine synthetase; and glycine amidinotransferase) and two metabolic pathways (butanoate metabolism and dicarboxylate metabolism) related to KYDS, to our knowledge, had been newly discovered in our study. The mechanism of action mainly involved energy metabolism, oxidative stress, ammonia metabolism, amino acid metabolism, and fatty acid metabolism. In short, our study demonstrated that targets and metabolic pathways for the treatment of KYDS by YGP can be effectively found via combining with systems pharmacology and urinary metabonomics. In addition to this, common and specific targets and metabolic pathways of KYDS treated by YGP can be found effectively by integration with the analysis of different biological samples (e.g., serum, urine, feces, and tissue). It is; therefore, important that this laid the foundation for deeper mechanism research and drug-targeted therapy of KYDS in future.

scholarly journals 1H NMR studies on serum metabonomic changes over time in a kidney-Yang deficiency syndrome model

RSC Advances ◽  
2017 ◽  
Vol 7 (54) ◽  
pp. 34251-34261 ◽  
Author(s):  
Ruiqun Chen ◽  
Jia Wang ◽  
Chengbin Liao ◽  
Na Ma ◽  
Lei Zhang ◽  
...  
Keyword(s):  
1H Nmr ◽  
Metabolic Pathways ◽  
Deficiency Syndrome ◽  
Nmr Studies ◽  
Kidney Yang Deficiency ◽  
Changes Over Time ◽  
Over Time

The central aim of this study was to investigate metabolite changes in metabolic pathwaysviametabonomic approaches in rats suffering from Kidney-Yang Deficiency Syndrome (KYDS) induced by hydrocortisone.

scholarly journals Nontarget Metabolomics of Grape Seed Metabolites Produced by Various Scion–Rootstock Combinations

2020 ◽  
Vol 145 (4) ◽  
pp. 247-256
Author(s):  
Zhijun Zhang ◽  
Huaifeng Liu ◽  
Junli Sun ◽  
Songlin Yu ◽  
Wang He ◽  
...  
Keyword(s):  
Discriminant Analysis ◽  
Least Squares ◽  
Partial Least Squares ◽  
Metabolic Pathways ◽  
Tricarboxylic Acid Cycle ◽  
Principal Component ◽  
Grape Seed ◽  
Grape Seeds ◽  
And Control

The use of resistant rootstocks is an inevitable trend in the development and production of grapes (Vitis sp.). The present study analyzed differences in the metabolites in grape seeds of different rootstock combinations (1103P, 5C, SO4, 3309C, 140R, and control) grafted onto ‘Cabernet Sauvignon’ (CS) wine grape (Vitis vinifera) scions (control, CS/CS, self-rooted grafting vines) using liquid chromatography–mass spectrometry (LC-MS) and nontargeted metabolomic techniques. Principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA), and orthogonal-partial least squares discriminant analysis identified 30 significant metabolites and 22 metabolic pathways in the seeds of CS that differed significantly from the control seeds. This study revealed that rootstocks influence metabolite concentrations and metabolic pathways (alanine–aspartate–glutamate pathway, arginine-proline pathway, and the tricarboxylic acid cycle) in the scion onto which they are grafted. The rootstocks increased the concentration of delphinidin-3-(6-acetylglucoside), peonidin 3-(6-p-coumarylglucoside), L-threonine, and D-tartaric in CS seeds. Appropriate rootstock combinations can be used to improve the quality of grape seeds by changing the concentrations of amino acids, organic acids, polyphenols, and vitamin B. This study provides a theoretical basis for selecting grape rootstocks and provides important insights for improving the quality of commercial products derived from grape seeds.

scholarly journals The Role of Cell Metabolism in Innate Immune Memory

2020 ◽  
pp. 1-9
Author(s):  
Anaisa Valido Ferreira ◽  
Jorge Domiguéz-Andrés ◽  
Mihai Gheorghe Netea
Keyword(s):  
Metabolic Pathways ◽  
Tricarboxylic Acid Cycle ◽  
Cell Metabolism ◽  
Innate Immune ◽  
Immune Memory ◽  
Functional Changes ◽  
Trained Immunity ◽  
Health And Disease

Immunological memory is classically attributed to adaptive immune responses, but recent studies have shown that challenged innate immune cells can display long-term functional changes that increase nonspecific responsiveness to subsequent infections. This phenomenon, coined <i>trained immunity</i> or <i>innate immune memory</i>, is based on the epigenetic reprogramming and the rewiring of intracellular metabolic pathways. Here, we review the different metabolic pathways that are modulated in trained immunity. Glycolysis, oxidative phosphorylation, the tricarboxylic acid cycle, amino acid, and lipid metabolism are interplaying pathways that are crucial for the establishment of innate immune memory. Unraveling this metabolic wiring allows for a better understanding of innate immune contribution to health and disease. These insights may open avenues for the development of future therapies that aim to harness or dampen the power of the innate immune response.

scholarly journals Role of Glutathione in Male Infertility

2013 ◽  
Vol 4 (2) ◽  
pp. 20-25
Author(s):  
ZU Naher ◽  
SK Biswas ◽  
FH Mollah ◽  
M Ali ◽  
MI Arslan
Keyword(s):  
Male Infertility ◽  
Seminal Plasma ◽  
Antioxidant Defense ◽  
Sperm Morphology ◽  
Sperm Count ◽  
Antioxidant Systems ◽  
Infertile Male ◽  
Semen Volume ◽  
Infertile Group ◽  
Male Subjects

Infertility is a worldwide problem and in almost 50% of cases infertility results from abnormality of the male partners. Apart from endocrine disorders, definitive cause and mechanism of male infertility is not clear in many cases. Recent evidence indicates that imbalance between pro-oxidant stress and antioxidant defense plays an important role in the pathogenesis of male infertility. Among the endogenous antioxidant systems, reduced glutathione (GSH) plays a significant role in the antioxidant defense of the spermatogenic epithelium, the epididymis and perhaps in the ejaculated spermatozoa. The current study was therefore designed to evaluate any association that may exist between GSH levels and male infertility. Infertile male patients (having female partners with normal fertility parameters; n=31) and age- matched healthy male fertile control subjects (n=30) were included in this study. In addition to medical history, semen analyses including semen volume, sperm count, motility and morphology were done for each subject. As a measure of antioxidant capacity erythrocyte and seminal plasma GSH concentrations were measured by Ellman's method in fertile and infertile male subjects. The infertile subjects were similar to fertile subjects in terms of age. However, semen volume and sperm count was found significantly lower (p<0.001) in infertile males compared with healthy fertile male subjects. Percentage of subjects with abnormal sperm morphology and motility were found higher in infertile group compared with fertile group. The median (range) erythrocyte GSH level did not differ between the two groups (12.62 (0.67-29.82) versus 13.93 (2.10-21.08) mg/gm Hb). However, the seminal plasma GSH level was found markedly suppressed in infertile group (1.64 (0.23-7.50)) compared with fertile group (4.26 (2.32-7.50)) mg/dl (p<0.001). In the present study seminal plasma GSH level was found markedly suppressed along with abnormal values for semen volume, sperm concentration and sperm morphology and motility in infertile subjects compared with fertile subjects. This finding indicates that low level of seminal plasma GSH level may be associated with male infertility. DOI: http://dx.doi.org/10.3329/bjmb.v4i2.13772 Bangladesh J Med Biochem 2011; 4(2): 20-25

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