scholarly journals Phenolic Rich Extract fromClinacanthus nutansAttenuates Hyperlipidemia-Associated Oxidative Stress in Rats

2016 ◽  
Vol 2016 ◽  
pp. 1-16 ◽  
Author(s):  
Nadarajan Sarega ◽  
Mustapha Umar Imam ◽  
Der-Jiun Ooi ◽  
Kim Wei Chan ◽  
Norhaizan Md Esa ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
High Fat Diet ◽  
Protocatechuic Acid ◽  
Synergistic Effects ◽  
Leaf Extracts ◽  
Antioxidant Genes ◽  
Stress Markers ◽  
Antioxidant Capacities ◽  
Clinacanthus Nutans ◽  
And Oxidative Stress

Clinacanthus nutansis used as traditional medicine in Asia but there are limited scientific studies to support its use. In this study, the stem and leaf ofC. nutanswere extracted using solvents of differing polarities, and their antioxidant capacities were determined using multiple antioxidant assays. The water and aqueous methanolic leaf extracts were further fractionated and their antioxidant capacities and phenolic compositions were tested. Furthermore, the efficacies of the water and aqueous methanolic leaf extracts were tested against hyperlipidemia-induced oxidative stress in rats. Serum and hepatic antioxidant and oxidative stress markers were tested after feeding the rats with high fat diet together with the extracts or simvastatin for 7 weeks. The results indicated that both leaf extracts attenuated oxidative stress through increasing serum antioxidant enzymes activity and upregulating the expression of hepatic antioxidant genes. Multiple phenolic compounds were detected in the extracts and fractions ofC. nutans, although protocatechuic acid was one of the most abundant and may have contributed significantly towards the bioactivities of the extracts. However, synergistic effects of different phenolics may have contributed to the overall bioactivities.C. nutanscan be a good source of functional ingredients for the management of oxidative stress-related diseases.

scholarly journals One Week of CDAHFD Induces Steatohepatitis and Mitochondrial Dysfunction with Oxidative Stress in Liver

2021 ◽  
Vol 22 (11) ◽  
pp. 5851
Author(s):  
Takehito Sugasawa ◽  
Seiko Ono ◽  
Masato Yonamine ◽  
Shin-ichiro Fujita ◽  
Yuki Matsumoto ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Mitochondrial Dysfunction ◽  
High Fat Diet ◽  
Early Stage ◽  
Droplet Deposition ◽  
Nonalcoholic Fatty Liver ◽  
Stress Markers ◽  
Mitochondrial Dna Copy Number ◽  
Short Period ◽  
And Oxidative Stress

The prevalence of nonalcoholic fatty liver disease (NAFLD) has been rapidly increasing worldwide. A choline-deficient, L-amino acid-defined, high-fat diet (CDAHFD) has been used to create a mouse model of nonalcoholic steatohepatitis (NASH). There are some reports on the effects on mice of being fed a CDAHFD for long periods of 1 to 3 months. However, the effect of this diet over a short period is unknown. Therefore, we examined the effect of 1-week CDAHFD feeding on the mouse liver. Feeding a CDAHFD diet for only 1-week induced lipid droplet deposition in the liver with increasing activity of liver-derived enzymes in the plasma. On the other hand, it did not induce fibrosis or cirrhosis. Additionally, it was demonstrated that CDAHFD significantly impaired mitochondrial respiration with severe oxidative stress to the liver, which is associated with a decreasing mitochondrial DNA copy number and complex proteins. In the gene expression analysis of the liver, inflammatory and oxidative stress markers were significantly increased by CDAHFD. These results demonstrated that 1 week of feeding CDAHFD to mice induces steatohepatitis with mitochondrial dysfunction and severe oxidative stress, without fibrosis, which can partially mimic the early stage of NASH in humans.

scholarly journals One Week Feeding of CDAHFD Induces Steatohepatitis and Mitochondrial Dysfunction with Oxidative Stress in Liver

2021 ◽  
Author(s):  
Takehito Sugasawa ◽  
Seiko Ono ◽  
Masato Yonanine ◽  
Shin-ichiro Fujita ◽  
Yuki Matsumoto ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Mitochondrial Dysfunction ◽  
High Fat Diet ◽  
Early Stage ◽  
Nonalcoholic Fatty Liver ◽  
Stress Markers ◽  
Mitochondrial Dna Copy Number ◽  
Short Period ◽  
Long Time ◽  
And Oxidative Stress

The prevalence of nonalcoholic fatty liver disease (NAFLD) has been rapidly increasing worldwide. A choline-deficient L-amino acid-defined high fat diet (CDHFD) has been used to create a mouse model of nonalcoholic steatohepatitis (NASH). There are some reports about the effects on mice of being fed CDAHFD for a long time, 1 to 3 months. However, the effect of this diet over a short period has been unknown. Therefore, we examined the effect of one week of feeding CDAHFD on the mouse liver. Feeding this diet for only one week induced lipid droplet deposition in the liver with increasing activity of liver-derived enzymes in the plasma. On the other hand, it did not induce fibrosis and cirrhosis. Additionally, it was demonstrated that mitochondrial respiration is significantly impaired with severe oxidative stress in the liver by CDAHFD, associated with a decreasing mitochondrial DNA copy number and complexes-proteins. In the gene expression analysis of the liver, inflammatory and oxidative stress markers were significantly increased by CDAHFD. These results demonstrated that one week of feeding CDAHFD to mice induces steatohepatitis with mitochondrial dysfunction and severe oxidative stress, without fibrosis, which can partially mimic the early stage of the NASH in humans.

scholarly journals Metabolic and oxidative stress markers in Wistar rats after 2 months on a high-fat diet

2014 ◽  
Vol 6 (1) ◽  
pp. 130 ◽  
Author(s):  
Nathalie Auberval ◽  
Stéphanie Dal ◽  
William Bietiger ◽  
Michel Pinget ◽  
Nathalie Jeandidier ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
High Fat Diet ◽  
Wistar Rats ◽  
Oxidative Stress Markers ◽  
High Fat ◽  
Stress Markers ◽  
And Oxidative Stress

scholarly journals Dietary Annatto-Extracted Tocotrienol Reduces Inflammation and Oxidative Stress, and Improves Macronutrient Metabolism in Obese Mice: A Metabolic Profiling Study

Nutrients ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 1267
Author(s):  
Chwan-Li Shen ◽  
Sivapriya Ramamoorthy ◽  
Gurvinder Kaur ◽  
Jannette M. Dufour ◽  
Rui Wang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
High Fat Diet ◽  
Metabolic Disorders ◽  
Essential Amino Acids ◽  
Obese Mice ◽  
High Fat ◽  
Toxic Dose ◽  
Serum Metabolites ◽  
Stress Markers ◽  
And Oxidative Stress

Obesity and its related complications are a world-wide health problem. Dietary tocotrienols (TT) have been shown to improve obesity-associated metabolic disorders, such as hypercholesterolemia, hyperglycemia, and gut dysbiosis. This study examined the hypothesis that the antioxidant capacity of TT alters metabolites of oxidative stress and improves systemic metabolism. C57BL/6J mice were fed either a high-fat diet (HFD control) or HFD supplemented with 800 mg annatto-extracted TT/kg (HFD+TT800) for 14 weeks. Sera from obese mice were examined by non-targeted metabolite analysis using UHPLC/MS. Compared to the HFD group, the HFD+TT800 group had higher levels of serum metabolites, essential amino acids (lysine and methionine), sphingomyelins, phosphatidylcholine, lysophospholipids, and vitamins (pantothenate, pyridoxamine, pyridoxal, and retinol). TT-treated mice had lowered levels of serum metabolites, dicarboxylic fatty acids, and inflammatory/oxidative stress markers (trimethylamine N-oxide, kynurenate, 12,13-DiHOME, and 13-HODE + 9-HODE) compared to the control. The results suggest that TT supplementation lowered inflammation and oxidative stress (oxidized glutathione and GSH/GSSH) and improved macronutrient metabolism (carbohydrates) in obese mice. Thus, TT actions on metabolites were beneficial in reducing obesity-associated hypercholesterolemia/hyperglycemia. The effects of a non-toxic dose of TT in mice support the potential for clinical applications in obesity and metabolic disease.

scholarly journals ACE Reduces Metabolic Abnormalities in a High-Fat Diet Mouse Model

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Seong-Jong Lee ◽  
Jong-Min Han ◽  
Jin-Seok Lee ◽  
Chang-Gue Son ◽  
Hwi-Jin Im ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
High Fat Diet ◽  
Serum Lipid ◽  
Peroxisome Proliferator ◽  
Metabolic Abnormalities ◽  
Oxidative Stress Markers ◽  
High Fat ◽  
Peroxisome Proliferator Activated Receptor ◽  
Stress Markers ◽  
And Oxidative Stress

The medicinal plantsArtemisia iwayomogi(A. iwayomogi) andCurcuma longa(C. longa) radix have been used to treat metabolic abnormalities in traditional Korean medicine and traditional Chinese medicine (TKM and TCM). In this study we evaluated the effect of the water extract of a mixture ofA. iwayomogiandC. longa(ACE) on high-fat diet-induced metabolic syndrome in a mouse model. Four groups of C57BL/6N male mice (except for the naive group) were fed a high-fat diet freely for 10 weeks. Among these, three groups (except the control group) were administered a high-fat diet supplemented with ACE (100 or 200 mg/kg) or curcumin (50 mg/kg). Body weight, accumulation of adipose tissues in abdomen and size of adipocytes, serum lipid profiles, hepatic steatosis, and oxidative stress markers were analyzed. ACE significantly reduced the body and peritoneal adipose tissue weights, serum lipid profiles (total cholesterol and triglycerides), glucose levels, hepatic lipid accumulation, and oxidative stress markers. ACE normalized lipid synthesis-associated gene expressions (peroxisome proliferator-activated receptor gamma, PPARγ; fatty acid synthase, FAS; sterol regulatory element-binding transcription factor-1c, SREBP-1c; and peroxisome proliferator-activated receptor alpha, PPARα). The results from this study suggest that ACE has the pharmaceutical potential reducing the metabolic abnormalities in an animal model.

scholarly journals High-fat diet-induced metabolic syndrome and oxidative stress in obese rats are ameliorated by yogurt supplementation

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Shoumen Lasker ◽  
Md Mizanur Rahman ◽  
Faisal Parvez ◽  
Mushfera Zamila ◽  
Pintu Miah ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Metabolic Syndrome ◽  
High Fat Diet ◽  
Alternative Therapy ◽  
High Fat ◽  
Stress Markers ◽  
Diet Induced Obesity ◽  
Obese Rats ◽  
Male Wistar Rats ◽  
And Oxidative Stress

AbstractThe main objective of this experiment was to determine the effects of yogurt supplementation on fat deposition, oxidative stress, inflammation and fibrosis in the liver of rats with high-fat (HF) diet-induced obesity. Male Wistar rats were used in this study and were separated into the following four different groups: the control, control + yogurt, high fat and high fat+ yogurt groups. The high fat groups received a HF diet for eight weeks. A 5% yogurt (w/w) supplement was also provided to rats fed the HF diet. Yogurt supplementation prevented glucose intolerance and normalized liver-specific enzyme activities in the HF diet-fed rats. Yogurt supplementation also significantly reduced the levels of oxidative stress markers in the plasma and liver of HF diet-fed rats. Moreover, inflammatory cell infiltration, collagen deposition and fibrosis in the liver of HF diet-fed rats were also prevented by yogurt supplementation. Furthermore, yogurt supplementation normalized the intestinal lining and brush border in HF diet-fed rats. This study suggests that yogurt supplementation potentially represents an alternative therapy for the prevention of metabolic syndrome in HF diet-fed rats.

TOXICOLOGICAL IMPLICATIONS OF COMBINED CONSUMPTION OF HIGH FAT DIET AND ALCOHOL IN RATS

2018 ◽  
Vol 24 (2) ◽  
Author(s):  
HEMESHWER KUMAR CHANDRA ◽  
GITA MISHRA ◽  
NISHA SAHU ◽  
SATENDRA KUMAR NIRALA ◽  
MONIKA BHADAURIA
Keyword(s):  
Oxidative Stress ◽  
High Fat Diet ◽  
Biochemical Parameters ◽  
Renal Toxicity ◽  
Control Group ◽  
High Fat ◽  
Stress Markers ◽  
Liver And Kidney ◽  
Serum Transaminases ◽  
And Oxidative Stress

The aim of the present study was to study the combined toxic effect of high fat diet and alcohol consumption on serological and oxidative stress markers in rats. Rats in control group received regular diet and water for 8 weeks, toxicant group were administered with 30% HFD and 10% alcohol for 2, 4, 6 and 8 weeks respectively.Combined consumption of high fat diet along with alcohol induced hepato-renal toxicity as evident by elevation of serum transaminases, alkaline phosphatase, bilirubin, cholesterol and triglycerides with significant decrease in glucose and albumin. Increased urea and uric acid was also seen in serum. Boosted lipid peroxidation and tissue biochemical parameters and decline in glutathione contents confirmed oxidative stress induced hepatorenal toxicity. Present study showed that 2, 4, 6 and 8 weeks administration of high fat diet along with alcohol significantly disturbed physiological integrity of liver and kidney.

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