scholarly journals Rutin Attenuates Hepatotoxicity in High-Cholesterol-Diet-Fed Rats

2016 ◽  
Vol 2016 ◽  
pp. 1-11 ◽  
Author(s):  
Shakir D. AlSharari ◽  
Salim S. Al-Rejaie ◽  
Hatem M. Abuohashish ◽  
Mohamed M. Ahmed ◽  
Mohamed M. Hafez
Keyword(s):  
Signaling Pathway ◽  
Transforming Growth Factor Beta ◽  
Transforming Growth Factor ◽  
Kinase Inhibitor ◽  
High Cholesterol Diet ◽  
Protective Agent ◽  
Cholesterol Diet ◽  
High Cholesterol ◽  
Expression Levels ◽  
Male Wistar Rats

Background and Objective.High-cholesterol diet (HCD) intends to increase the oxidative stress in liver tissues inducing hepatotoxicity. Rutin is a natural flavonoid (vitamin p) which is known to have antioxidative properties. The aim of the present study was to investigate the potential effects of Rutin on hypercholesterolemia-induced hepatotoxicity in rats.Materials and Methods.Male Wistar rats were divided into four groups: G-I control, G-II Rutin, G-III HCD, and G-IV Rutin + HCD. The liver functions and lipid profile were used to evaluate the HCD-induced hepatotoxicity. Quantitative real time-PCR was carried out to evaluate the expression levels of genes in TGF-β/Smad signaling pathway.Results.Rutin in combination with HCD showed a significant protective effect against hepatotoxicity. HCD caused significant increase in the mRNA expression of transforming growth factor beta (TGF-β), Mothers Against Decapentaplegic Homolog 2 (Smad-2), Mothers Against Decapentaplegic Homolog 4 (Smad-4), Bcl-2-binding component 3 (Bbc3),caspase-3,P53and Interleukin-6 (IL-6) and decrease in the expression levels of Cyclin depended kinase inhibitor (P21) and Interleukin-3(IL-3) in hepatic cells.Conclusion. TGF-β/Smadsignaling pathway is involved in HCD-induced hepatotoxicity and Rutin inhibits the hepatotoxicity via suppressing this pathway. Therefore, Rutin might be considered as a protective agent for hepatotoxicity.

scholarly journals Eruberin A, a Natural Flavanol Glycoside, Exerts Anti-Fibrotic Action on Pancreatic Stellate Cells

2015 ◽  
Vol 36 (6) ◽  
pp. 2433-2446 ◽  
Author(s):  
Siu Wai Tsang ◽  
Hong-Jie Zhang ◽  
Ye-Gao Chen ◽  
Kathy Ka-Wai Auyeung ◽  
Zhao-Xiang Bian
Keyword(s):  
Signaling Pathway ◽  
Transforming Growth Factor Beta ◽  
Type I Collagen ◽  
Transforming Growth Factor ◽  
Smooth Muscle Actin ◽  
Stellate Cells ◽  
Pancreatic Stellate Cells ◽  
Type I ◽  
Alpha Smooth Muscle Actin ◽  
Expression Levels

Background: Eruberin A (2, 3-dehydroflavonoid), a flavanol glycoside isolated from Pronephrium penangianum, has been used as a blood-nourishing folk medicine for centuries; however, it indeed possesses a variety of other health-promoting benefits including anti-fibrotic bioactivity. Activation of pancreatic stellate cells (PSCs) is the key initiating step in pancreatic fibrosis, which is a characteristic feature associated with chronic pancreatitis and pancreatic adenocarcinoma. Methods: The anti-fibrotic effect of eruberin A and the underlying mechanisms of its anti-fibrotic action in LTC-14 cells, which retained essential characteristics and morphological features of primary PSCs, were examined by means of real-time polymerase chain reactions, Western blotting and immunostaining. Results: The application of eruberin A (20 µg/ml) effectively inhibited the expression levels of fibrotic mediators namely alpha-smooth muscle actin, fibronectin and type I-collagen, so as the sonic hedgehog signaling pathway components post transforming growth factor-beta (5 ng/ml) stimulation. Eruberin A treatment also led to a notable decrease in the activation of nuclear factor-kappaB (NF-κB) and the phosphorylation of phosphoinositide 3-kinase (PI3K)/serine-threonine kinase (AKT). Conclusion: Our results demonstrated that eruberin A significantly suppressed the expression levels of fibrotic mediators in PSCs, and we suggest that its anti-fibrotic mechanism was associated with an attenuation of the PI3K/AKT/NF-κB signaling pathway.

scholarly journals Pengaruh Lama Pemberian Diet Tinggi Kolesterol terhadap Kadar LDL dan TGF-Β Serum Tikus Putih (Rattus novergicus) strain Wistar

2016 ◽  
Vol 5 (1) ◽  
Author(s):  
Biomechy Oktomalioputri ◽  
Eryati Darwin ◽  
Eva Decroli
Keyword(s):  
Growth Factor ◽  
Transforming Growth Factor ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Transforming Growth Factor Β ◽  
Control Group ◽  
High Cholesterol Diet ◽  
Low Density ◽  
Cholesterol Diet ◽  
High Cholesterol

AbstrakDiet tinggi kolesterol ini akan meningkatkan kadar Low Density Lipoprotein (LDL) sebagai penanda hiperlipidemia yang berdampak pada terjadinya aterosklerosis. Transforming Growth Factor β (TGF-β) memiliki peranan dalam proses terjadinya aterosklerosis ini. Keterlibatannya dalam hiperlipidemia sebagai faktor risiko utama aterosklerosis belum banyak diketahui. Tujuan penelitian ini adalah menentukan pengaruh lama permberian diet tinggi kolesterol terhadap kadar LDL dan TGF-β pada tikus putih (Rattus novergicus) strain Wistar. Penelitian ini menggunakan metode post test only control group design yang dilakukan terhadap tikus Rattus novergicus jantan umur 3-4 bulan, berat 200-250 gram. Sampel penelitian terdiri dari 24 ekor tikus yang dibagi menjadi 4 kelompok, yaitu kelompok kontrol, A, B dan C. Selain kelompok kontrol, kelompok tikus diberi diet tinggi kolesterol berupa lemak kambing 10%, telur puyuh 5%, selama 10 hari untuk kelompok A, 20 hari untuk kelompok B dan 30 hari untuk kelompok C. Pada akhir percobaan darah tikus diambil dan dilakukan pemeriksaan kadar LDL dan TGF-β serum. Hasil penelitian diolah secara bivariat. Analisis yang digunakan yaitu uji oneway Anova. Hasil penelitian diketahui terdapat pengaruh lama pemberian diet tinggi kolesterol terhadap peningkatan kadar LDL serum tikus dengan p=0,01 (p<0,05). Terdapat pengaruh lama pemberian diet tinggi kolesterol terhadap penurunan kadar TGF-β dimana p=0,04 (p>0,05). Penelitian ini menyimpulkan bahwa terdapat pengaruh lama pemberian diet tinggi kolesterol terhadap kadar LDL dan tikus putih Rattus novergicus strain Wistar.Kata kunci: diet tinggi kolesterol, LDL, TGF-β AbstractHigh-cholesterol diet will increase Low Density Lipoprotein (LDL) levels which impact to atherosclerosis. Transforming Growth Factor β (TGF-β) play a role in atherosclerosis process. But its involvement in hyperlipidemia as the main risk factor of atherosclerosis still unknown. The objective of this study was to observe the effect of duration of giving  high-cholesterol diet on Low Density Lipoprotein and Transforming Growth Factor β levels white rats (Rattus novergicus) Wistar strain. This study uses  post-test only control group design, carried out on male rats Rattus novergicus 3-4 months, weight 200-250 grams. The study sample consisted of 24 rats were divided into 4 groups: control group, group A, group B and group C. Except  the control group, the group of rats given a high-cholesterol diet in the form of goat fat 10%, 5% quail eggs for 10 days to group A, group B for 20 days and 30 days for group C. At the end of the experiment blood was taken and examined LDL and TGF-β levels. Results were analyzed using bivariate. The analysis is oneway Anova test. The results of research known to have effect of duration on giving high-cholesterol diet to increase serum LDL levels of rats with p = 0.01 (p<0.05). This is the same case with TGF-β, which there was effect of duration on giving high-diet cholesterol diet to decrease serum TGF-β levels of rats where p = 0.04 (p>0.05). This study concludes that there are effect of duration on giving high-cholesterol diet to levels of LDL and TGF-β white rats Rattus novergicus Wistar strain.Keywords:  high-cholesterol diet, LDL, TGF β

Synergistic effect of Betulinic acid, Apigenin and Skimmianine (BASk) in high cholesterol diet rabbit: Involvement of CD36-TLR2 signaling pathway

Cytokine ◽  
2021 ◽  
Vol 142 ◽  
pp. 155475
Author(s):  
Vidya Sabu ◽  
Santhi Krishnan ◽  
Jasmine Peter ◽  
I.S Aswathy ◽  
S.S Lal Preethi ◽  
...  
Keyword(s):  
Synergistic Effect ◽  
Signaling Pathway ◽  
Betulinic Acid ◽  
High Cholesterol Diet ◽  
Cholesterol Diet ◽  
High Cholesterol ◽  
Tlr2 Signaling

Hypolipidemic and antioxidant effect of Ajuga iva in rats fed a high-cholesterol diet

Planta Medica ◽  
2006 ◽  
Vol 72 (11) ◽  
Author(s):  
MA Lacaille-Dubois ◽  
A Chenni ◽  
DA Yahia ◽  
FO Boukortt ◽  
J Prost ◽  
...  
Keyword(s):  
Antioxidant Effect ◽  
High Cholesterol Diet ◽  
Cholesterol Diet ◽  
High Cholesterol
Sign in / Sign up

Export Citation Format

Share Document