scholarly journals An Attempt to Establish a Common Animal Model for Hepatorenal Fibrosis in Rats

2017 ◽  
Vol 2017 ◽  
pp. 1-10
Author(s):  
Manoj Hang Limbu ◽  
Liu Lei ◽  
Cheng Zhengyuan ◽  
Liu Jing ◽  
Zhang Xiaoyi ◽  
...  
Keyword(s):  
Animal Model ◽  
Liver Disease ◽  
Carbon Tetrachloride ◽  
Wistar Rats ◽  
Medical Science ◽  
Important Research ◽  
Kidney Fibrosis ◽  
Disease Condition ◽  
Female Wistar Rats ◽  
Liver And Kidney

It is already a proven fact that there exists a relationship between CLD (chronic liver disease) and kidney disease but still there is no available combined animal model of liver and kidney fibrosis on the same animal. An animal model is one of the important research tools in the field of medical science because it is important to build a model that can simulate the disease condition so that the particular disease can be studied. Therefore, the aim of this study is to build a less expensive, less time consuming, and reproducible model of hepatorenal fibrosis on rats. We administered combined intraperitoneal injection of CCl4 (Carbon Tetrachloride) and BSA (Bovine Serum Albumin) on a female Wistar rats. At the end, the liver and kidney tissues were examined under microscope to see whether we were successful in establishing the model or not. The results show that liver fibrosis was marked but the changes on the kidneys were mild. In this study, we were able to induce significant fibrosis in the liver and early stages of fibrosis in the kidneys. The result also demonstrated that the addition of BSA conferred a liver protective effect against CCl4 induced hepatotoxicity, whereas combination of CCl4 and BSA proved to be detrimental for kidneys.

Lactobacillus protected bone damage and maintained the antioxidant status of liver and kidney homogenates in female wistar rats

2012 ◽  
Vol 368 (1-2) ◽  
pp. 155-165 ◽  
Author(s):  
Sarika Amdekar ◽  
Avnish Kumar ◽  
Poonam Sharma ◽  
Rambir Singh ◽  
Vinod Singh
Keyword(s):  
Antioxidant Status ◽  
Wistar Rats ◽  
Bone Damage ◽  
Female Wistar Rats ◽  
Liver And Kidney

scholarly journals Comparisons of Curative Effects of Chlorophyll fromSauropus androgynus(L) Merr Leaf Extract and Cu-Chlorophyllin on Sodium Nitrate-Induced Oxidative Stress in Rats

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Suparmi Suparmi ◽  
Minidian Fasitasari ◽  
Martanto Martosupono ◽  
Jubhar Christian Mangimbulude
Keyword(s):  
Oxidative Stress ◽  
Sodium Nitrate ◽  
Wistar Rats ◽  
Histological Evaluation ◽  
Female Wistar Rats ◽  
Male Wistar Rats ◽  
Liver And Kidney ◽  
Serum Biochemical ◽  
Hb Concentration ◽  
Rbc Count

Sodium nitrate (NaNO2) widely used as food additive for coloring and preserving meat has been reported to induce oxidative stress and cause histopathologic changes, nitrosative tissue damage, and lipid peroxidation in liver and kidney. Therefore, the present study compared the curative effect of chlorophyll fromSauropus androgynus(L) Merr and Cu-chlorophyllin as antioxidant in NaNO2-induced female Wistar rats based on haematological, serum biochemical, and histological evaluation. Thirty male Wistar rats were randomly assigned into six groups of five rats each. NaNO2were given at a subacute dose of 50 mg/kg bw intraperitoneally for 10 days. Chlorophyll fromS. androgynusand Cu-chlorophyllin from K-Liquid™ were given in the following 14 days at the two doses: 0,016 mg/mL and 0.008 mg/mL. NaNO2exposure resulted in significant reductions (p<0.05) in values of packed cell volume (PCV), haemoglobin (Hb) concentration and red blood cell (RBC) count, transferrin, and ferritin and elevation in malondialdehyde (MDA) level and schistocytes percentage with insignificant reductions in serum albumin and transferrin levels. Histology of kidney and liver were changed insignificantly (p>0.05) to normal values. Chlorophyll fromS. androgynusand Cu-chlorophyllin possess antioxidant potentials to protect against toxicities induced by sodium nitrate.

scholarly journals Modeling of Chemoperfusion vs. Intravenous Administration of Cisplatin in Wistar Rats: Adsorption and Tissue Distribution

Molecules ◽  
2020 ◽  
Vol 25 (20) ◽  
pp. 4733
Author(s):  
Galina Kireeva ◽  
Stepan Kruglov ◽  
Mikhail Maydin ◽  
Ekaterina Gubareva ◽  
Elena Fedoros ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Fold Increase ◽  
Systemic Circulation ◽  
Efficacy And Safety ◽  
Total Blood ◽  
Pharmacokinetic Assessment ◽  
Female Wistar Rats ◽  
Liver And Kidney ◽  
To Receive ◽  
Intraperitoneal Chemoperfusion

Hyperthermic intraperitoneal chemoperfusion (HIPEC) is an established form of locoregional chemotherapy of peritoneum tumors. However, its efficacy and safety status remain a controversy, partially, due to scarce data on pharmacokinetics and toxicity profile of drugs under HIPEC. In the current study, 24 female Wistar rats were randomly assigned to receive cisplatin as HIPEC (n = 12, 20 mg/kg) or intravenously (i.v., n = 9, 4 mg/kg). The subgroups of three animals were used for the initial, intermediate, and late phases of the pharmacokinetic assessment. The animals were sacrificed on days 1 and 5. Blood, liver, kidney, and ovaries were evaluated for platinum content. Histological and immunohistochemical evaluation was undertaken in the liver and kidney. A trend for higher blood plasma platinum levels was observed for HIPEC compared to i.v. Significantly lower (p < 0.001) relative platinum binding to the proteins was observed in HIPEC animals compared to the i.v. administration. A five-fold higher concentration of cisplatin in HIPEC resulted in a ca. 2.5-fold increase in total blood platinum and ca. two-fold increase in blood ultrafitrable platinum (“free” Pt). Immunohistochemistry revealed higher kidney and liver damage after i.v. administration of cisplatin compared to HIPEC, although a five-fold higher dose of cisplatin was applied in HIPEC. Together with relatively lower absorption to the systemic circulation in HIPEC, higher protein binding is probably the primary reason for lower observed toxicity in HIPEC animals.

Amla (Emblica officinalis) improves hepatic and renal oxidative stress and the inflammatory response in hypothyroid female wistar rats fed with a high-fat diet

2018 ◽  
Vol 29 (2) ◽  
pp. 175-184 ◽  
Author(s):  
P. Rajaa Muthu ◽  
Zachariah Bobby ◽  
P. Sankar ◽  
V. Vickneshwaran ◽  
Sajini Elizabeth Jacob
Keyword(s):  
Oxidative Stress ◽  
Inflammatory Response ◽  
High Fat Diet ◽  
Wistar Rats ◽  
Molecular Techniques ◽  
Emblica Officinalis ◽  
High Fat ◽  
Tnf Α ◽  
Female Wistar Rats ◽  
Liver And Kidney

AbstractBackground:We investigated the protective effects of amla (Emblica officinalis) on the pathogenesis of oxidative stress (OS) and inflammatory response in hypothyroid rats fed with a high-fat diet (HFD) as an experimental model of hypothyroidism (HT) with obesity.Methods:A total of 80 female wistar rats (5-months-old) were divided into eight different groups. Propylthiouracil (PTU) and HFD were used to induce the experimental HT and obesity, respectively. The euthyroid and hypothyroid rats were fed either normal chow or HFD with and without amla extract (AE, 100 mg/kg bw/day) for 6 weeks. The blood and tissues, liver and kidney OS and inflammatory parameters were studied using appropriate biochemical and molecular techniques.Results:PTU and HFD per se caused OS and inflammatory response as evidenced by increased plasma MDA, TNF-α, CRP and GPx in association with decreased levels of TAS and reduced glutathione (GSH). The proteomic analysis revealed that the expressions of pERK, pP38, TNF-α, IL6, COX2 and NOX-4 were up-regulated in the liver and kidney of these rats. In addition, all these metabolic derangements were further augmented when HT was followed by the addition of HFD. This suggested that there was a synergism between HT and the intake of HFD on the development of OS and inflammatory response.Conclusions:The treatment with amla fruit extract significantly restored the redox imbalance and inflammatory signaling and ameliorated OS and inflammatory response, suggesting the use of this natural compound as an alternative remedy or adjuvant for the management of metabolic complications concomitant with HT.

scholarly journals The protective effect of Vitamin D and combination with liv-52 on lipid profiles in carbon tetrachloride-induced liver disease in Wistar rats

2021 ◽  
Vol 12 (11) ◽  
pp. 71-78
Author(s):  
K. Ponnazhagan ◽  
J. Sivakumar ◽  
T. Gopalakrishnan
Keyword(s):  
Vitamin D ◽  
Liver Disease ◽  
Carbon Tetrachloride ◽  
Free Cholesterol ◽  
Wistar Rats ◽  
Total Body Weight ◽  
Well Being ◽  
The Body ◽  
Total Body ◽  
Exogenous Substances

Background: Liver is the largest gland, accounts for approximately 2.5% of total body weight and also liver called as the metabolic “engine-room of the body.” Liver plays role in both metabolism as well as biochemical transformation. Therefore, it is vital to maintain a healthy liver for overall health and well-being. However, liver is continuously exposed to exogenous substances such as toxins, drugs, and alcohol which can ultimately lead to various liver disorders. Liver diseases today are one of the most fatal diseases globally. Aims and Objectives: The aim of the present study was to evaluate lipid peroxidation (LPO) and lipid profile levels of Vitamin D and combination with Liv-52 on carbon tetrachloride induced liver disease in rats. Materials and Methods: Thirty-six adult male Albino Wistar rats weighing 150–200 g were used in this study. Liver disease was induced in rats by administration of Carbon tetrachloride (CCl4) intraperitoneally at the dose of 1 mL mixed with 50% of olive oil twice a week for 5 weeks, 3 days interval between each dose, after confirmation of liver disease treated with Vitamin D and Liv-52 for 5 weeks. Results: The levels of LPO were significantly decreased in Vitamin D and Liv-52 treated animals when compared with CCl4 induced animals. Total cholesterol, free cholesterol, phospholipids, and triglycerides (TGs) levels were significantly reduced when compared with CCl4 induced rats. However, the levels of ester cholesterol and free fatty acids (FFAs) were significantly increased in Vitamin D and Liv-52 treated animals when compared with CCl4 induced animals. Conclusion: Vitamin D and Liv-52 effectively reduced the LPO levels. Treatment with Vitamin D and Liv-52, the levels of total and free cholesterol, phospholipids, and TGs were significantly reduced and treatment with both combinations highly reduced all these levels when compared with CCl4 induced animals. And treatment with Vitamin D and Liv-52, the ester cholesterol and FFAs levels were increased.

scholarly journals Safety Assessment of a New Strain of Lactobacillus pentosus (IBRC=11143) As a Candidate Probiotic

2021 ◽  
Author(s):  
Saba Bahrehvar ◽  
Shiva Khezri ◽  
Amir Abbas Barzegari ◽  
Vahid Nejati
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Wistar Rats ◽  
Blood Parameters ◽  
Male Rats ◽  
Human Consumption ◽  
Lactobacillus Pentosus ◽  
Female Wistar Rats ◽  
Liver And Kidney ◽  
The Right

Background: It is believed that the consumption of an adequate amount of live lactic acid bacteria (as probiotic) may improve the health of the host. Many strains of lactic acid bacteria are generally considered to be safe. However, some strains have shown some adverse effects. Objectives: This study aimed to investigate the safety of a new strain of Lactobacillus pentosus (IBRC=11143) in Wistar rats Methods:In this experimental study, male and female Wistar rats (n=6) were used. A subacute toxicity study (for 28 days) was conducted by oral administration of Lactobacillus pentosus to the animals. In each sex, one group received saline, and the other two groups received the bacteria at doses of 1×108 and 1×109 Colony-Forming Units (CFU)/rat. Results: No significant alteration in the liver and kidney tissues was seen. However, in both sexes, there were significant differences in urea and creatinine levels between the control and the experimental groups. Some blood parameters (Lymphocyte, red blood cell, hematocrit, and hemoglobin) also showed significant changes in the groups that received the bacteria. Moreover, a significant increase in alkaline phosphatase level was observed in male rats. Conclusion: The results indicate that Lactobacillus pentosus (11143) is not entirely safe like other Lactobacillus strains. Therefore, the Lactobacillus pentosus (11143) strain may not be the right choice as a probiotic for human consumption.

scholarly journals Subchronic Toxicity of Crinum jagus Extracts on Wistar Rats

2021 ◽  
Vol 6 (1) ◽  
pp. 117-120
Author(s):  
Mvongo Clemence ◽  
Kamgang Rene ◽  
Essame Oyono Jean-Louis
Keyword(s):  
Total Cholesterol ◽  
Wistar Rats ◽  
Folk Medicine ◽  
Relative Weight ◽  
Term Treatment ◽  
Total Proteins ◽  
Subchronic Toxicity ◽  
Long Term Treatment ◽  
Female Wistar Rats ◽  
Liver And Kidney

Crinum jagus is used in Cameroon western and eastern regions folk medicine in detoxification, the management of diabetes and obesity and also as antivenomous and antipoison. The aim of this work was to evaluate the subchronic toxicity of Crinum jagus extracts on male and female Wistar rats. Subchronic toxicity of aqueous and hydroéthanolic Crinum jagus extracts was determined on two month old normal Wistar rats. Those rats, once daily orally received, hydroethanolic (75, 150 mg/kg b.w.) and aqueous (150 mg/kg b.w.) extracts, during 90 days (tree month). In both males and females animals, the C. jagus effects were investigated on the evolution of weight, food and water intakes, kidney and liver functioning markers (serum total cholesterol (TC), total proteins, creatinine and transaminase: AST and ALT). Both aqueous and hydroethanolic extracts in males as in females, did not cause any adverse changes in anthropometric (body mass, relative weight of liver and kidneys, food and water intakes) and seric parameters (total cholesterol, total proteins, creatinine AST and ALT activity). Instead, those extracts remarkably improved antropometric parameters, liver and kidney function and even protect again atherosclerosis. The results indicated that aqueous and hydroethanolic extracts of Crinum jagus did not induce toxic effect at the doses used in long term treatment; thus justifying its empiric use in detoxification and as antivenomous.

Changes in kynurenine pathway metabolism in the brain, liver and kidney of aged female Wistar rats

FEBS Journal ◽  
2011 ◽  
Vol 278 (22) ◽  
pp. 4425-4434 ◽  
Author(s):  
Nady Braidy ◽  
Gilles J. Guillemin ◽  
Hussein Mansour ◽  
Tailoi Chan-Ling ◽  
Ross Grant
Keyword(s):  
Wistar Rats ◽  
Kynurenine Pathway ◽  
Female Wistar Rats ◽  
Liver And Kidney ◽  
The Brain
Sign in / Sign up

Export Citation Format

Share Document