scholarly journals CHEMICAL COMPOSITION, LARVICIDAL ACTIVITY AND RESIDUAL EFFECT OF Pterodon polygalaeflorus (BENTH.) BENTH. (FABACEAE) FRUIT OIL EXTRACTS AGAINST Aedes aegypti (DIPTERA: CULICIDAE)

2020 ◽  
Vol 48 (4) ◽  
Author(s):  
Armanda Amando Teles de Menezes ◽  
Tayrrana Silva Beltrão ◽  
Liliane Sousa Silva ◽  
Heloisa Helena Garcia da Silva ◽  
Ionizete Garcia da Silva ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Aedes Aegypti ◽  
Larvicidal Activity ◽  
Chemical Characterization ◽  
Residual Effect ◽  
Small Scale ◽  
Mosquito Vector ◽  
Germacrene D ◽  
Oil Extract ◽  
Fruit Oil

The purpose of this study was to investigate the larvicidal activity of Pterodon polygalaeflorus oil extract against the mosquito vector Aedes aegypti. For this, crushed P. polygalaeflorus fruit underwent solvent extraction to obtain the oil extract. The chemical characterization was performed by gas chromatography coupled to mass spectrometry. For the bioassays third instar larvae of Ae. aegypti were utilized. Tests were carried out to determine the larvicidal activity and the residual effect under laboratory conditions, as well as field screening (small scale).The major components of P. polygalaeflorus oil extract were, respectively, E-caryophyllene, germacrene D and bicyclogermacrene. Lethal concentrations of 50% and 90% were 36.5 and 64.8 μg/mL respectively. The solution presented a residual effect for seven days and the efficiency of the product was preserved under field conditions. The results encourage continuing studies with the oil extract of P. polygalaeflorus as a research target for bioinsecticides.KEY WORDS: Bioinsecticide; sucupira; terpenes; vector control.

scholarly journals Residual Larvicidal Activity of Quinones against Aedes aegypti

Molecules ◽  
2020 ◽  
Vol 25 (17) ◽  
pp. 3978 ◽  
Author(s):  
Raquel L. Silva ◽  
Daniel P. Demarque ◽  
Renata G. Dusi ◽  
João Paulo B. Sousa ◽  
Lorena C. Albernaz ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Yellow Fever ◽  
Larvicidal Activity ◽  
Residual Effect ◽  
Small Scale ◽  
Limited Information ◽  
Effective Measure ◽  
Arboviral Diseases ◽  
Effect Parameter ◽  
Leading Compounds

The number of documented dengue cases has increased dramatically in recent years due to transmission through the Aedes aegypti mosquito bite. Vector control remains the most effective measure to protect against this and other arboviral diseases including Zika, chikungunya and (urban) yellow fever, with an established vaccine only available for yellow fever. Although the quinone class shows potential as leading compounds for larvicide development, limited information restricts the development of optimized structures and/or formulations. Thus, in this contribution we investigated the larvicidal and pupicidal activity of three quinone compounds isolated from a Connarus suberosus root wood ethyl acetate extract together with 28 quinones from other sources. Eight quinones demonstrated larvicidal activity, of which tectoquinone (4) proved to be the most active (LC50 1.1 µg/mL). The essential residual effect parameter of four of these quinones was evaluated in laboratory trials, with tectoquinone (4) and 2-ethylanthraquinone (7) presenting the most prolonged activity. In small-scale field residual tests, tectoquinone (4) caused 100% larvae mortality over 5 days, supporting its selection for formulation trials to develop a prototype larvicide to control Ae. aegypti.

scholarly journals Larvicidal activity of oil-resin fractions from the Brazilian medicinal plant Copaifera reticulata Ducke (Leguminosae-Caesalpinoideae) against Aedes aegypti (Diptera, Culicidae)

2007 ◽  
Vol 40 (3) ◽  
pp. 264-267 ◽  
Author(s):  
Heloísa Helena Garcia da Silva ◽  
Regina Geris ◽  
Edson Rodrigues Filho ◽  
Cleonice Rocha ◽  
Ionizete Garcia da Silva
Keyword(s):  
Mass Spectrometry ◽  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Aedes Aegypti ◽  
Larvicidal Activity ◽  
Control Methods ◽  
Alternative Control ◽  
13C Nuclear Magnetic Resonance ◽  
Layer Chromatography ◽  
Alternative Control Methods

Oil-resin fractions from Copaifera reticulata Ducke (Leguminosae-Caesalpinoideae) were evaluated for larvicidal activity on third larval instars of Aedes aegypti, in searching for alternative control methods for this mosquito. The bioactive fractions were chemically monitored by thin-layer chromatography, ¹H and 13C nuclear magnetic resonance and mass spectrometry. Bioassays were performed using five repetitions, at a temperature of 28 ± 1°C, relative humidity of 80 ± 5% and light and dark cycles of 12h. Mortality was indicated by darkening of the cephalic capsule after 24h of exposure of the larvae to the solutions. The most active fractions were CRM1-4 (sesquiterpenes) and CRM5-7 (labdane diterpenes), which showed LC50 values of 0.2 and 0.8ppm, respectively.

scholarly journals Larvicidal Effect of Vinca Fruit Extract (Vinca rosea) Against Aedes aegypti Larvae and Secondary Metabolites Profile by Thin Layer Chromatography

2014 ◽  
Vol 3 (1) ◽  
pp. 31
Author(s):  
Rahmawati Ekaputri ◽  
Sudarsono Sudarsono ◽  
Budi Mulyaningsih
Keyword(s):  
Aedes Aegypti ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Larvicidal Activity ◽  
Larval Mortality ◽  
Probit Analysis ◽  
Mosquito Vector ◽  
Fruit Extract ◽  
Vinca Rosea ◽  
Layer Chromatography

<p>Background: <em>Vinca rosea</em> is known contain alkaloids, it was usually used to treat various diseases. Alkaloids from Vinca leaves are also already known have larvicidal activity. Based on this toxicological activity, the fruit of <em>Vinca rosea</em> was selected to investigation its larvicidal activity against the 3<sup>rd</sup> instar larvae of the mosquito vector of dengue haemorrhagic fever (DHF) <em>Aedes aegypti.</em>  Five concentrations of Vinca fruit extract were tested against the 3<sup>rd</sup> instar Aedes aegypti larvae. The different larval mortality percentages were recorded after 24 hours. Lethal concentration (LC<sub>50</sub> anf LC<sub>90</sub>) of Vinca fruit extract were calculated using Probit analysis. Phytochemical compounds  of ethanolic extract also investigated using Thin layer Chromatography (TLC). LC<sub>50</sub> and LC<sub>90</sub> values of fruit extract were 2.987 mg/ml and 32.861 mg/ml. Alkaloids were detected in extract.</p>

scholarly journals Larvicidal Activity against Aedes aegypti and Chemical Characterization of the Inflorescences of Tagetes patula

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Letícia Maria Krzyzaniak ◽  
Tânia Mara Antonelli-Ushirobira ◽  
Gean Panizzon ◽  
Ana Luiza Sereia ◽  
José Roberto Pinto de Souza ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Aedes Aegypti ◽  
Larvicidal Activity ◽  
Insecticidal Activity ◽  
Chemical Characterization ◽  
Reversed Phase ◽  
Acetone Extract ◽  
Tagetes Patula ◽  
Larval Population

The crude acetone extract (CAE) of defatted inflorescences of Tagetes patula was partitioned into five semipurified fractions: n-hexane (HF), dichloromethane (DF), ethyl acetate (EAF), n-butanol (BF), and aqueous (AQF). BF was fractionated by reversed-phase polyamide column chromatography, obtaining 34 subfractions, which were subjected to HSCCC, where patuletin and patulitrin were isolated. CAE and the fractions BF, EAF, DF, and AQF were analyzed by LC-DAD-MS, and patuletin and patulitrin were determined as the major substances in EAF and BF, respectively. BF was also analyzed by HPLC and capillary electrophoresis (CE), and patulitrin was again determined to be the main substance in this fraction. CAE and the semipurified fractions (750, 500, 300, 100, and 50 mg/L) were assayed for larvicidal activity against Aedes aegypti, with mortality rate expressed as percentage. All fractions except AQF showed insecticidal activity after 24 h exposure of larvae to the highest concentration. However, EAF showed the highest activity with more than 50% reduction in larval population at 50 mg/L. The insecticidal activity observed with EAF might have been due to the higher concentration of patuletin present in this fraction.

scholarly journals Efektivitas Tablet Bacillus thuringiensis H-14 Isolat Salatiga terhadap Jentik Aedes aegypti pada Dua Sumber Air yang Berbeda

2021 ◽  
pp. 1-10
Author(s):  
Arum Triyas Wardani ◽  
RA. Wigati ◽  
Esti Rahardianingtyas ◽  
Rendro Wianto ◽  
Arief Nugroho
Keyword(s):  
Aedes Aegypti ◽  
Field Experiments ◽  
Slow Release ◽  
Residual Effect ◽  
Water Sources ◽  
Residual Effects ◽  
Well Water ◽  
Mosquito Vector ◽  
Control Effort ◽  
Significant Difference

Dengue Hemorrhagic Fever (DHF) is a vector-borne disease that is still a health problem in Indonesia. One of the DHF control effort is to control the larvae of the mosquito vector using Bacillus thuringensis H-14. The weakness of this metode is its short residual effect.  This study aims to determine differences in the effectiveness of B. thuringiensis H-14 tested in two different water sources, namely well water and PDAM water in people's houses and its residual effects in the field. The study used a quasi-​experimental, which include laboratory and field experiments in March to September 2017. The research was carried out by producing B. thuringensis H-14 Salatiga isolates in the form of slow release tablets, testing the efficacy and measuring their effectiveness to Aedes aegypti mosquito larvae in two kinds of water sources. The results showed that the content of B. thuringiensis Salatiga isolate in slow release tablets weighing 125 mg was 5 mg. The efficacy test at laboratory scale showed that the LC50 was 0.436 ppm and the LC90 was 2.440 ppm. The effectiveness test in the field of LC50 (PDAM water) is 0.098 ppm and (well water) is 1.909 ppm, while LC90 (PDAM water) is 0.186 ppm and (well water) is 0.909 ppm. The efficacy assay results showed there was no significant difference in the effect of B. thuringiensis tablet between well water and PDAM water. B. thuringiensis H-14 tablets were effective in controlling Ae. aegypti larvae more than 80% until the 7th day in field testing.

scholarly journals Larvicidal Activity of Essential Oils from the Leaves and Fruits of Nutmeg (Myristica fragrans Houtt) Against Aedes aegypti (Diptera: Culicidae)

2016 ◽  
Vol 4 (7) ◽  
pp. 552
Author(s):  
Anne Carolina ◽  
Maman Maman
Keyword(s):  
Essential Oil ◽  
Aedes Aegypti ◽  
Larvicidal Activity ◽  
Steam Distillation ◽  
Chemical Compounds ◽  
Chemical Components ◽  
Myristica Fragrans ◽  
Leaf Oil ◽  
Myristica Fragrans Houtt ◽  
Fruit Oil

The aim of this research was to examine the larvicidal activity of essential oil (EO) extracted from nutmeg (Myristica fragrans Houtt) leaves and fruits by steam distillation, and to analyze its chemical compounds. The EO yield of nutmeg leaves and fruits collected from the same tree was 0.66% and 0.30%, respectively. Larvicidal tests with the EO were carried out against Aedes aegypti (L.) (Diptera: Culicidae). The concentrations of nutmeg EO used for the larvicidal assay were 50, 100, 150, 200, and 250 μg/mL. The results showed that fruit oil was more toxic than the leaf oil. LC50 values of leaf and fruit EOs were 133.8 and 110.1 µg/mL, respectively. The chromatogram of GC-MS showed that the chemical components in nutmeg leaf and fruit EOs were dominated by α-pinene, sabinene, β-pinene, delta-3-carene, limonene, β-phellandrene, α-terpinolene, linalool, safrole, croweacin, and myristicin.

scholarly journals Screening of Different Extracts of Marine Macro Green Algae for Larvicidal Activity against Dengue Fever Mosquito, Aedes aegypti (Diptera: Culiadae)

2017 ◽  
Vol 62 ◽  
pp. 44-51
Author(s):  
G. Adaikala Raj ◽  
M. Jayaraman ◽  
S. Krishnamoorthy ◽  
M. Chandrasekaran ◽  
V. Venkatesalu
Keyword(s):  
Aedes Aegypti ◽  
Ethyl Acetate ◽  
Green Algae ◽  
Larvicidal Activity ◽  
Ethyl Acetate Extract ◽  
Larval Mortality ◽  
Control Agent ◽  
Probit Analysis ◽  
Mosquito Vector ◽  
Ethyl Acetate Extracts

The present study larvicidal activities of hexane, chloroform, ethyl acetate, acetone and methanol extracts of Halimeda macroloba, Decsne, Caulerpa racemosa (Frosk) Weber-Van-Bosses and Ulva lactuca Lin, (Chlorophyceae) against Aedes aegypti. The marine macro green algae extracts were tested against early 4th instar larvae of A. aegypti using WHO protocol and concentrations of 200, 400, 600, 800 and 1000 ppm. The observed mortality was made 24 and 48 h after treatment, data was subjected to probit analysis to determine the lethal concentration (LC50 and LC90) of the treated larvae of the tested species.Among the tested extracts the maximum efficicacy was observed in the ethyl acetate extracts. The ethyl acetate extracts of the seaweeds showed the presence of terpenoids, tannins and phenolic compounds stronger than the other extracts. The results revealed that all the extracts showed varied levels of larvicidal activity against A. aegypti tested. However, the ethyl acetate extract of C. racemosa showed remarkable larvicidal activity against A. aegypti (LC50=579.9 and LC90=1255.4 ppm values at 24 h and LC50 =495.4 and LC90 =1073.9 ppm at 48 h) followed by U. lactuca (LC50=588.1 and LC90=1290.7 ppm values at 24 h, and LC50= 530.8 and LC90= 1160.0 ppm at 48 h), respectively. The lowest larval mortality was observed with hexane extract of H. macroloba against A. aegypti with values of LC50=1116.8 and LC90= 1824.5 ppm (after 24 h) and LC50=1059.9 and LC90=1768.3 ppm (after 24 h). The present studies indicate that the larvicidal activity and phytochemicals derived from the ethyl acetate extract of C. racemosa have the potential to be used as an ideal eco-friendly approach and effective mosquito vector control agent.

scholarly journals Inga laurina crude extract to control Aedes aegypti

2020 ◽  
Vol 9 (11) ◽  
pp. e1819119683
Author(s):  
Elisangela Yumi Sugauara ◽  
Isabelle Luiz Rahal ◽  
Herika Line Marko de Oliveira ◽  
Wanessa de Campos Bortolucci ◽  
Carla Maria Mariano Fernandez ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Aedes Aegypti ◽  
Larvicidal Activity ◽  
Crude Extract ◽  
Chemical Identification ◽  
Third Stage ◽  
Insecticide Activity ◽  
Stage Development ◽  
Extracting Solvent

Aedes aegypti is the um mosquito responsible for the transmission of several diseases to humans such as dengue, chikungunya, Zika virus and urban yellow fever. The control of this culicid is done by utilizing insecticides that cause insect resistance. Therefore, natural alternatives to control A. aegypti have been sought. Thus, the objective of our work was to characterize the chemical composition of the crude extract of the leaves of Inga laurina and to evaluate the insecticidal activity of the extract on the larvae of A. aegypti. The crude extract was prepared form dry leaves by dynamic maceration using alcohol 96º GL as extracting solvent. The chemical identification of the compounds found in the crude extract was done by a gas chromatography coupled to mass spectrometry. The larvicidal activity was determined by larval packet test in third-stage development larvae A. aegypti. Fifteen compounds were identified in the leaves and the major ones were γ- sitosterol (34.39%), phytol (14.51%), squalene (8.57%) and stigmasterol (7.38%). I. laurina leaf crude extract presented larvicidal activity potential against A. aegypti larvae presenting lethal concentration of 50% (CL50) of 0.98 mg/mL and 99% (CL99) of 2.69 mg/mL. Thus, I. laurina leaf crude extract presented rich composition of phytosterols and promising insecticide activity against A. aegypti larvae, offering new possibilities for the application and development of products.

scholarly journals Larvicidal activity of vegetable oils against Aedes aegypti larvae

2021 ◽  
Vol 74 (2) ◽  
Author(s):  
Francisco Roberto De Azevedo ◽  
Lays Laianny Amaro Bezerra ◽  
Toshik Iarley Da Silva ◽  
Renato Augusto Da Silva ◽  
José Valmir Feitosa
Keyword(s):  
Aedes Aegypti ◽  
Larvicidal Activity ◽  
Vegetable Oils ◽  
Ricinus Communis ◽  
Cocos Nucifera ◽  
Mosquito Vector ◽  
Carapa Guianensis ◽  
Randomized Design ◽  
Copaifera Langsdorffii ◽  
And Control

Aedes aegypti L. is the mosquito vector of yellow fever, dengue, zika, and chikungunya viruses. The prevention and control of such diseases usually rely on the use of chemicals, that can cause harm to human health and the environment. Vegetable oils with larvicidal activity are used as an alternative tool to control this insect. This study aimed to evaluate the larvicidal activity of vegetable oils from Caryocar coriaceum, Mauritia fIexuosa, Carapa guianensis, Copaifera langsdorffii, Ricinus communis and Cocos nucifera against A. aegypti larvae. The experiment was divided into two bioassays. In the first, a completely randomized design was used with seven treatments (six vegetable oils at 500 ppm and one control with four replications). The number of dead larvae was evaluated 24, 48, 72, 96, and 120 h after exposure. In the second bioassay, the most efficient vegetable oils from the first bioassay (C. coriaceum and M. flexuosa) were used at the concentrations of 0, 500, 1000, 1500, 2000, and 2500 ppm, with four replications. The number of dead larvae was evaluated according to the first bioassay. All oils used had larvicidal activity on third instar stage larvae of A. aegypti, with greater efficiency 120 h after exposure. The oils of C. coriaceum and M. flexuosa at 2500 ppm had the best efficacy in the larvae control. The LD10, LD50, and LD90 of M. flexuosa oil recommended for controlling larvae are 234, 648, and 1794 ppm, respectively.

scholarly journals Comprehensive quantitative proteome analysis of Aedes aegypti identifies proteins and pathways involved in Wolbachia pipientis and Zika virus interference phenomenon

2020 ◽  
Author(s):  
Michele Martins ◽  
Luis Felipe Costa Ramos ◽  
Jimmy Rodriguez Murillo ◽  
André Torres ◽  
Stephanie Serafim de Carvalho ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Immune Response ◽  
Aedes Aegypti ◽  
Humoral Immune Response ◽  
Zika Virus ◽  
Mosquito Vector ◽  
Ros Production ◽  
Zikv Infection ◽  
Wolbachia Pipientis ◽  
Humoral Immune

ABSTRACTZika virus is a global public health emergency due to its association with microcephaly, Guillain-Barré syndrome, neuropathy, and myelitis in children and adults. A total of 87 countries have had evidence of autochthonous mosquito-borne transmission of Zika virus, distributed across four continents, and no antivirus therapy or vaccines are available. Therefore, several strategies have been developed to target the main mosquito vector, Aedes aegypti, to reduce the burden of different arboviruses. Among such strategies, the use of the maternally-inherited endosymbiont Wolbachia pipientis has been applied successfully to reduce virus susceptibility and decrease transmission. However, the mechanisms by which Wolbachia orchestrate resistance to ZIKV infection remain to be elucidated. In this study, we apply isobaric labeling quantitative mass spectrometry-based proteomics to quantify proteins and identify pathways altered during ZIKV infection; Wolbachia infection; co-infection with Wolbachia/ZIKV in the Ae. aegypti heads and salivary glands. We show that Wolbachia regulates proteins involved in ROS production, regulates humoral immune response, and antioxidant production. The reduction of ZIKV polyprotein in the presence of Wolbachia in mosquitoes was determined by mass spectrometry and corroborates the idea that Wolbachia helps to block ZIKV infections in Ae. aegypti. The present study offers a rich resource of data that may help to elucidate mechanisms by which Wolbachia orchestrate resistance to ZIKV infection in Ae. aegypti, and represents a step further on the development of new targeted methods to detect and quantify ZIKV and Wolbachia directly in complex tissues.HighlightsThe abundance of ZIKV polyprotein is reduced in the presence of WolbachiaShotgun proteomics quantifies ZIKV and Wolbachia proteins directly in tissuesWolbachia regulates proteins involved in ROS productionWolbachia regulates humoral immune response and antioxidant productionMetabolism and detoxification processes were associated with mono infections

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