scholarly journals The Effects of Aloe vera Cream on the Expression of CD4+ and CD8+ Lymphocytes in Skin Wound Healing

2018 ◽  
Vol 2018 ◽  
pp. 1-5 ◽  
Author(s):  
Yos Adi Prakoso ◽  
Kurniasih
Keyword(s):  
Wound Healing ◽  
Topical Application ◽  
Aloe Vera ◽  
Skin Wound ◽  
Control Group ◽  
Histopathological Study ◽  
Sprague Dawley ◽  
Skin Wound Healing ◽  
Group I ◽  
Significant Difference

The aim of this study is to explore the effect of topical application of Aloe vera on skin wound healing. Thirty-six male Sprague-Dawley rats weighing 150–200 grams were divided into four groups. All groups were anesthetized, shaved, and exposed to round full-thickness punch biopsy on the back: group I (control); group II (treated with 1% Aloe vera cream); group III (treated with 2% Aloe vera cream); and group IV (treated with madecassol®). The treatments were given once a day. Macroscopic and microscopic examination were observed at 5, 10, and 15 days after skin biopsy. Skin specimens were prepared for histopathological study using H&E stain and IHC stain against CD4+ and CD8+ lymphocytes. All the data were analyzed using SPSS16. The result showed that topical application of 1% and 2% Aloe vera cream significantly reduced the percentage of the wound, leucocytes infiltration, angiogenesis, and expression of CD8+ lymphocytes and increased the epidermal thickness and the expression of CD4+ lymphocytes (p ≤ 0,05). There was no significant difference in the number of fibroblasts in all groups. Topical application of 1% and 2% Aloe vera cream has wound healing potential via their ability to increase the ratio of CD4+/CD8+ lymphocytes in the wound area.

Perbedaan Pengaruh Aplikasi Gel Kombinasi Kitosan Berat Molekul Tinggi dan Rendah dengan Ekstrak Lidah Buaya (Aloe vera) Terhadap Kepadatan Kolagen pada Proses Penyembuhan Ulkus Traumatikus

DENTA ◽  
2018 ◽  
Vol 12 (1) ◽  
pp. 60
Author(s):  
Sularsih Sularsih ◽  
Michelle Suhartono ◽  
Nafi’ah Nafi’ah
Keyword(s):  
Molecular Weight ◽  
Wound Healing ◽  
High Molecular Weight ◽  
Aloe Vera ◽  
Collagen Fibers ◽  
Control Group ◽  
Low Molecular Weight ◽  
Group I ◽  
Significant Difference ◽  
Low Molecular Weight Chitosan

<p><strong><em>Background:</em></strong><em> Traumatic ulcer is one of the most common oral wounds. Chitosan has mucoadhesive characteristic while Aloe vera containing lignin which is able to penetrate the skin. It is expected that the combined gel of chitosan and Aloe vera will function as wound healing accelerator in traumatic ulcer. Molecular weight is one of the characteristics of chitosan quality. <strong>Purpose:</strong> the aim of this experiment was to know the density of collagen fibers in wound healing of traumatic ulcer using the combined gel of chitosan with different molecular weight and Aloe vera. <strong>Materials and method: </strong>30 Male Rattus Norvegicus were divided into 3 groups. Group I was control group (without chitosan and Aloe vera), group II was given low molecular weight chitosan and Aloe vera, group III was given high molecular weight chitosan and Aloe vera. The groups were given traumatic ulcer making with 4 mm diameter and 2 mm depth. Rats were sacrificed by decapitation on day 3 and 7 then they were examined histopatologically to see the density of collagen fibers. <strong>Result:</strong> Statistical analysis with Kruskall Wallis and Mann-Whitney U test showed that there were significant difference p&lt;0,05 between high and low molecular weight chitosan with Aloe vera group on day 3 and 7. <strong>Conclusion:</strong> Chitosan with high molecular weight and Aloe vera were more effective in wound healing of traumatic ulcer b</em><em>ecause they increase</em><em> the density of collagen fibers.</em><em></em></p><p><strong><em> </em></strong></p><p><strong><em>Keywords:</em></strong><em>  Combined gel of chitosan and Aloe vera, density of collagen fibers, wound healing.</em><em></em></p><p><strong><em> </em></strong></p><strong><em>Korespondensi:</em></strong><em> Sularsih, Bagian Ilmu Biomaterial Kedokteran Gigi, Fakultas Kedokteran Gigi, Universitas Hang Tuah, Arif Rahman Hakim 150, Telepon 031-5912191.</em>

scholarly journals Effect of Curcuma longa Crude Extract, Curcumin and Nano Curcumin-based Gel Topical Administration on Excised Skin Wound Healing in Swiss-Webster Mice

2021 ◽  
Vol 14 (02) ◽  
pp. 635-641
Author(s):  
Afiat Berbudi ◽  
Ali Akbar Fadlillah ◽  
Merry Afni ◽  
Nur Atik
Keyword(s):  
Wound Healing ◽  
Topical Application ◽  
Curcuma Longa ◽  
Area Under The Curve ◽  
Topical Administration ◽  
Treated Group ◽  
Healing Time ◽  
Control Group ◽  
Skin Wound Healing ◽  
Significant Difference

Patients suffering from skin wounds often have their life heavily affected economically and socially. In general, wound healing consists of four different sequentially and overlapping phases. Interrupting those phases results in extending the healing time. Turmeric (Curcuma longa) is widely used by Indonesians to treat wounds, with curcumin as an active substance, which is proven to have various effects on improving wound healing. Therefore, this study aims to determine how the topical application of turmeric gel on affects the acceleration of wound closure. Analytical laboratory experiments were conducted on 24 male Swiss-Webster mice. Subjects were divided into four groups according to where the excision wounds were made on the back, and observed on days 0, 3, 6, 9, 12, and 15 post wound induction. The wound healing acceleration was observed after 3 (p = 0.0003), 6 (p = 0.0085), and 9 (p = 0.0023) days in C. longa-treated mice compared to the control group. However, there was no significant difference found on days 12 and 15 after wounding. In addition, the area under the curve analysis shows significant difference between the C. longa-treated group compared to the control, but not with the group treated with neither curcumin nor nano curcumin gel. In conclusion, topical application of 3% C. longa extract gel improves excised skin healing.

scholarly journals Evaluation of the alcoholic extract of Dipteryx alata Vogel almonds and bark in skin wound healing in C57BL6 mice

2021 ◽  
Vol 73 (6) ◽  
pp. 1315-1322
Author(s):  
M.C.P. Gouveia ◽  
B.W. Minto ◽  
L.F. Sargi ◽  
R.L. Souza ◽  
J.M. Pazzini ◽  
...  
Keyword(s):  
Wound Healing ◽  
Topical Application ◽  
Healing Process ◽  
Skin Wound ◽  
Bark Extract ◽  
Wound Healing Process ◽  
Alcoholic Extract ◽  
Skin Wound Healing ◽  
Significant Difference ◽  
Dipteryx Alata

ABSTRACT The aim of this study was to evaluate the topical application of alcoholic extracts of Dipteryx alata Vogel almonds and bark in skin wound healing in mice. Fifty-four C57BL/6 mice were equally distributed into three groups: Control, Almond, and Bark. A 9 mm skin fragment was resected from the dorsal region of the animals’ thorax. The wounds were submitted to topical application of base cream (vehicle), 10% hydroalcoholic almond extract, or bark extract twice a day. Macroscopic, histological, and immunohistochemical evaluations were conducted on the 7th, 14th, and 21st postoperative days. No significant difference was observed regarding skin wound area among groups, with the parameter presenting only a temporal effect on healing (p>0.05). The almond and control groups exhibited more intense collagenization than the bark group (p<0.05). Dipteryx alata Vogel showed to be inert in the wound healing process in mice.

scholarly journals Expression of fibroblast growth factor-2 after application of the Queen's crepe-myrtle leaf (Lagerstroemia speciosa) and aloe vera extract gel in the wound healing process of hyperglycemic

2021 ◽  
Vol 33 (1) ◽  
pp. 48
Author(s):  
Fachrul Razi ◽  
Andri Hardianto ◽  
Lucky Riawan ◽  
Bambang Pontjo Priosoeryanto
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Aloe Vera ◽  
Simple Random Sampling ◽  
Fibroblast Growth Factor 2 ◽  
Control Group ◽  
Wound Healing Process ◽  
Sprague Dawley ◽  
Lagerstroemia Speciosa ◽  
Significant Difference

Introduction: Fibroblast Growth Factor-2 (FGF-2) have a role in stimulating the proliferation and migration of various types of cells in the wound healing process. Hyperglycemic conditions can disrupt the wound healing process. Lagerstroemia speciosa are known to have antyphyerglycemic and antioxidant effects. Aloe vera is a plant that has been used for long time in topical treatment of wounds.This study was aimed to analyse the FGF-2 expressions on the wound healing process of subjects with hyperglycemia after application of Queen's crepe-myrtle leaf (Lagerstroemia speciosa) and aloe vera extracts gel. Methods: A pure experimental study was conducted with simple random sampling. The sample was 27 experimental animals (CI 95%; power test 80%) of Sprague Dawley rat induced to have a hyperglycemic state using alloxan and had their palate injured. The immunohistochemical examination was performed to assess FGF-2 expression level. Samples were divided into three groups with a simple random sampling technique: nine of the rats received the Lagerstroemia speciosa extract gel application; nine rats received the aloe vera extract gel application on the injured palate; another nine rats in the control group. Examination of the FGF-2 expression level was performed on the third, seventh, and fourteenth observation days. Results: Overall, the control group had a significant difference with the Lagerstroemia speciosa and aloe vera extract gel group (p-value<0.05), however, the Lagerstroemia speciosa group did not have any significant difference with the aloe vera group (0.123>0.05). Conclusion: There was no difference in the amount of FGF-2 expressions on the wound healing process of the injured palatal tissue of Sprague-Dawley rats with hyperglycemic after application of the Lagerstroemia speciosa and aloe vera extract gel.

scholarly journals Comparison of effects of unfractionated heparin and low molecular weight heparin on skin wound healing of rats

2012 ◽  
Vol 27 (9) ◽  
pp. 639-644 ◽  
Author(s):  
Can Engin Durmaz ◽  
Aydin Ozkan ◽  
Bugra Senel ◽  
Hasan Alper Uyar
Keyword(s):  
Molecular Weight ◽  
Wound Healing ◽  
Unfractionated Heparin ◽  
Low Molecular Weight Heparin ◽  
Skin Wound ◽  
Control Group ◽  
Low Molecular Weight ◽  
Skin Wound Healing ◽  
Significant Difference ◽  
Experimental Group

PURPOSE: To compare the effects of unfractionated heparin (UH) and a low molecular weight heparin (LMWH) on skin wound healing of rats. METHODS: Forty eight male Sprague-Dawley rats underwent 8mm full thickness dorsal skin wounds and were randomly assigned to three equal groups. In experimental group A, heparin sodium was injected at a concentration of 1000U/kg. In experimental group B, enoxaparin was injected at a concentration of 1mg/kg. Physiologic saline (0.5ml) was administered to the control group. Injections were made subcutaneously, once daily, for seven days. At 7th and 10th days tissue samples were taken from all rats. Histologic examination of these tissues was made under light microscope and scored. RESULTS: Histological examination showed a significant difference between the 7th and 10th day groups in wound healing. It was observed that wound healing of LMWH injected group is better. This difference is statistically significant at 10th day. CONCLUSIONS: Daily administration of single doses of unfractionated heparin and a low molecular weight heparin improves wound healing positively. Low molecular weight heparin induces wound healing more than unfractionated heparin.

scholarly journals PENGARUH PEMBERIAN GEL KOMBINASI EKSTRAK GETAH PEPAYA (Carica papaya L) DAN EKSTRAK DAGING LIDAH BUAYA (Aloe vera) TERHADAP PROSES PENYEMBUHAN ULKUS TRAUMATIKUS PADA MALE WISTAR RATS YANG MENDERITA DIABETES MELITUS

2015 ◽  
Vol 2 (1) ◽  
pp. 41
Author(s):  
Umi Kulsum ◽  
Ratnawati Hendari ◽  
Siti Chumaeroh
Keyword(s):  
Wound Healing ◽  
Ulcer Healing ◽  
Wistar Rats ◽  
Povidone Iodine ◽  
Healing Process ◽  
Aloe Vera ◽  
Control Group ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Papaya Latex

Introduction: Research on the use of papaya and aloevera sap on wound healing each been done, but effects the combination of the two extracts on wound healing in diabetes mellitus condition has not been done.Purpose: This study aims to determine the effect of a gel combination of papaya latex and aloevera flesh extracts on the traumatics ulcer healing process in diabetic rats.Methods: True experimental laboratory research with the post-test-only control group design that performed on 28 male wistar rats which were divided intofour groups: DM and non-DM rat that treatment with povidone iodine and gel combination of papaya latex and aloevera flesh extract. The treatment was done for 7 days with smearing that was given 2x/day. Traumatics ulcer healing seen from the number of fibroblasts that then were analyzed by One WayAnova and post hoc LSD test.Results: The results showed the number of fibroblasts in DM and non-DM rat with povidone iodine were 44.6 and 42.73; in the DM and non-DM rat with a gel combination of papaya latex and aloe vera flesh extracts were 61.10 and 77.03. One Way Anova test resulted p-value = 0.000, it was mean that there were at least a significant difference on the number of fibroblasts in the two groups. Differences of the number fibroblasts was shown in all groups, except in the group DM and non-DM rats with povidone iodine (p = 0.764).Conclusion: Conclusion of the study was the provision of a gel combination of papaya latex and aloevera flesh extracts effect on the traumatics ulcer healing process in diabetic rats.

scholarly journals The Effect of Aloe Vera Extract to Extensive Lesion and Expression of Transforming Growth Factor (TGF-β) on Alkaline Chemical Trauma Cornea Model

2018 ◽  
Vol 1 (2) ◽  
pp. 1-17
Author(s):  
Fahcreza ◽  
Elsa Iskandar ◽  
Rachmat Hidayat ◽  
Petty Purwanita ◽  
Anang Tribowo ◽  
...  
Keyword(s):  
Wound Healing ◽  
Growth Factor ◽  
Wistar Rats ◽  
Aloe Vera ◽  
Control Group ◽  
Treatment Groups ◽  
White Rats ◽  
Significant Difference ◽  
The Difference ◽  
In Cells

Abstract Background: Chemical trauma to the cornea is an emergency condition of the eye that requires early diagnosis and good treatment. Alkaline have ability to saponify fatty acids in cells and cell membranes which can make penetration into the stroma and destroy proteoglycans and collagen in cells. Aloe vera (AV) contains several active substances that are reported to have anti-inflammatory, immunomodulatory, and wound healing effects. AV has been reported to accelerate the healing process of corneal epithelial defects by increasing fibroblast proliferation, collagen production and growth factor production. This study aims to determine the difference between the effect of aloe vera extract with a concentration of 10%, 20%, 40% and BSS on the healing of extensive corneal lesions in white wistar rats alkaline trauma models. Method: This study was an experimental study with a pre and posttest only with control group design in vivo approach to 30 Wistar white rats which were divided into 5 treatment groups for 3 days. Comparative analysis of effectiveness using the ANNOVA test or the Kruskal Wallis test and continued by the post hoc test. Results: Based on the one way ANOVA test there was a statistically significant difference in effectiveness between the five treatment groups on the percentage of corneal wound healing area and TGF-β expression with an assessment of p = 0,000 each. The administration of alloevera (AV) concentration of 20% had a significant difference in percentage of healing of corneal lesions and TGF-β expression compared with other treatment groups with p = 0,000 each. Large differences in the area of corneal lesions in the 40% AV group were -0.45 in the BBS group, 0.146 in the 10% AV group, 0.493 in the 20% AV group. The difference in the AV group 10% was 0.30 in the BBS group, -064 in the AV group 20%, and -0.14 in the AV group 40%. However, TGFβ expression in the normal control group that did not receive treatment was 54.94 (53.21-56-12). TGFβ levels in the BSS group were 10.44, the 10% aloe vera group was 25.43, 47.99 for the 20% aloe vera group and 37.95 for the 40% aloe vera group. Conclusion: There is a difference between the effect of aloe vera extract with concentrations of 10%, 20%, 40% and BSS on the extensive healing of corneal lesions in white wistar rats with alkaline chemical trauma models.

scholarly journals Effectiveness Of Topical Application Of Chlorhexidine And Prevailing Practices On Episiotomy Wound Among Postnatal Mothers

2014 ◽  
Vol 2 (1) ◽  
Author(s):  
Rincy Michael ◽  
Soney N Toppo ◽  
Varsha Hariharan
Keyword(s):  
Wound Healing ◽  
Topical Application ◽  
Controlled Trial ◽  
Demographic Data ◽  
Assessment Scale ◽  
Research Centre ◽  
Control Group ◽  
Significant Difference ◽  
Postnatal Mothers ◽  
Experimental Group

Puerperium is a period when the mother experiences intense physical and emotional stress due to exhaustion, anxiety and excitement. Each mother has to adjust to physical changes in her own body due to involution and lactation as well as cope up with the new demands on her time and emotions made by the new born baby. Episiotomy, despite evidences that it is an unnecessary intervention, is one of the most common surgical procedures performed during the second stage of labour, especially in primigravida woman. Thus, a randomized controlled trial to assess the effectiveness of topical application of chlorhexidine and prevailing practices on episiotomy wound among postnatal mothers at selected hospital of Indore was undertaken. True experimental approach with pre test post test research design was adopted. Simple random sampling was taken to select 40 postnatal mothers with episiotomy wound and three days postpartum hospital stay in Choithram Hospital & Research Centre, Indore as per the inclusion criteria. Data was collected with help of questionnaire for socio demographic data and obstetrical data and the wound healing was assessed by REEDA wound healing assessment scale. Samples in the experimental group were applied with chlorhexidine on episiotomy wound and the samples in the control group received routine care with betadine or nadoxin. Post assessment score was taken on third day (evening) by REEDA wound assessment scale. Finding of the study related to effectiveness of chlorhexidine among postnatal mothers in experimental group revealed that there was a significant difference in the healing of episiotomy wound by the application of chlorhexidine among postnatal mothers in experimental group at t19 = 11.40 at the level of p< 0.001. Hence it was inferred that topical application of chlorhexidine on episiotomy wound promotes wound healing.

scholarly journals EFFECTS OF PROBIOTICS SUPPLEMENTATION ON SKIN WOUND HEALING IN DIABETIC RATS

2020 ◽  
Vol 33 (1) ◽  
Author(s):  
Letícia Fuganti CAMPOS ◽  
Eliane TAGLIARI ◽  
Thais Andrade Costa CASAGRANDE ◽  
Lúcia de NORONHA ◽  
Antônio Carlos L. CAMPOS ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Wound Healing ◽  
Type I Collagen ◽  
Chronic Wounds ◽  
Skin Wound ◽  
Diabetic Rats ◽  
Control Group ◽  
Type I ◽  
Collagen Deposition ◽  
Skin Wound Healing

ABSTRACT Background: Chronic wounds in patients with Diabetes Mellitus often become incurable due to prolonged and excessive production of inflammatory cytokines. The use of probiotics modifies the intestinal microbiota and modulates inflammatory reactions. Aim: To evaluate the influence of perioperative supplementation with probiotics in the cutaneous healing process in diabetic rats. Methods: Forty-six rats were divided into four groups (C3, P3, C10, P10) according to the treatment (P=probiotic or C=control, both orally administered) and day of euthanasia, 3rd or 10th postoperative days. All rats were induced to Diabetes Mellitus 72 h before starting the experiment with alloxan. Supplementation was initiated five days before the incision and maintained until euthanasia. Scalpel incision was guided by a 2x2 cm mold and the wounds were left to heal per second-intention. The wounds were digitally measured. Collagen densitometry was done with Picrosirius Red staining. Histological parameters were analyzed by staining by H&E. Results: The contraction of the wound was faster in the P10 group which resulted in a smaller scar area (p=0.011). There was an increase in type I collagen deposition from the 3rd to the 10th postoperative day in the probiotic groups (p=0.016), which did not occur in the control group (p=0.487). The histological analysis showed a better degree of healing in the P10 group (p=0.005), with fewer polymorphonuclear (p<0.001) and more neovessels (p=0.001). Conclusions: Perioperative supplementation of probiotics stimulates skin wound healing in diabetic rats, possibly due to attenuation of the inflammatory response and increased neovascularization and type I collagen deposition.

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