Perbedaan Pengaruh Aplikasi Gel Kombinasi Kitosan Berat Molekul Tinggi dan Rendah dengan Ekstrak Lidah Buaya (Aloe vera) Terhadap Kepadatan Kolagen pada Proses Penyembuhan Ulkus Traumatikus

DENTA ◽  
2018 ◽  
Vol 12 (1) ◽  
pp. 60
Author(s):  
Sularsih Sularsih ◽  
Michelle Suhartono ◽  
Nafi’ah Nafi’ah
Keyword(s):  
Molecular Weight ◽  
Wound Healing ◽  
High Molecular Weight ◽  
Aloe Vera ◽  
Collagen Fibers ◽  
Control Group ◽  
Low Molecular Weight ◽  
Group I ◽  
Significant Difference ◽  
Low Molecular Weight Chitosan

<p><strong><em>Background:</em></strong><em> Traumatic ulcer is one of the most common oral wounds. Chitosan has mucoadhesive characteristic while Aloe vera containing lignin which is able to penetrate the skin. It is expected that the combined gel of chitosan and Aloe vera will function as wound healing accelerator in traumatic ulcer. Molecular weight is one of the characteristics of chitosan quality. <strong>Purpose:</strong> the aim of this experiment was to know the density of collagen fibers in wound healing of traumatic ulcer using the combined gel of chitosan with different molecular weight and Aloe vera. <strong>Materials and method: </strong>30 Male Rattus Norvegicus were divided into 3 groups. Group I was control group (without chitosan and Aloe vera), group II was given low molecular weight chitosan and Aloe vera, group III was given high molecular weight chitosan and Aloe vera. The groups were given traumatic ulcer making with 4 mm diameter and 2 mm depth. Rats were sacrificed by decapitation on day 3 and 7 then they were examined histopatologically to see the density of collagen fibers. <strong>Result:</strong> Statistical analysis with Kruskall Wallis and Mann-Whitney U test showed that there were significant difference p&lt;0,05 between high and low molecular weight chitosan with Aloe vera group on day 3 and 7. <strong>Conclusion:</strong> Chitosan with high molecular weight and Aloe vera were more effective in wound healing of traumatic ulcer b</em><em>ecause they increase</em><em> the density of collagen fibers.</em><em></em></p><p><strong><em> </em></strong></p><p><strong><em>Keywords:</em></strong><em>  Combined gel of chitosan and Aloe vera, density of collagen fibers, wound healing.</em><em></em></p><p><strong><em> </em></strong></p><strong><em>Korespondensi:</em></strong><em> Sularsih, Bagian Ilmu Biomaterial Kedokteran Gigi, Fakultas Kedokteran Gigi, Universitas Hang Tuah, Arif Rahman Hakim 150, Telepon 031-5912191.</em>

scholarly journals Uji Aktivitas Ekstrak Pericarp Kulit Buah Manggis Terpurifikasi Dalam Penyembuhan Luka Eksisi

2018 ◽  
Vol 1 (3) ◽  
pp. 134-138
Author(s):  
Dira Dira ◽  
Yanuarista Yanuarista ◽  
Ria Afrianti
Keyword(s):  
Wound Healing ◽  
Repeated Measures ◽  
Collagen Fibers ◽  
General Linear ◽  
Control Group ◽  
Garcinia Mangostana ◽  
Excision Wound ◽  
Group I ◽  
Significant Difference

Alfa mangostin memiliki berbagai macam bioaktivitas dan merupakan major compound dalam eksrak kulit manggis (Garcinia mangostana L.), alfa mangostin memiliki aktivitas sebagai antioksidan, antiinflamasi dan antibakteri sehingga berperan dalam proses penyembuhan luka. Penelitian ini bertujuan untuk mengamati aktivitas ekstrak pericarp kulit buah manggis terpurifikasi yang mengandung > 90% alfa-mangostin dalam penyembuhan luka eksisi pada mencit putih jantan secara in vivo. Paramater yang diukur adalah persentase penyembuhan luka eksisi, waktu epitelisasi dan kerapatan serabut kolagen. Mencit dibagi menjadi dua kelompok, dimana kelompok I merupakan kelompok kontrol negatif (-) yang hanya diberi sediaan suspensi Na CMC 1%, sedangkan kelompok II merupakan kelompok perlakuan yang diberi sediaan suspensi ekstrak dengan konsentrasi 1%. Pada hari ke-5, ke-10 dan ke-15 diukur persentase penyembuhan luka dan diamati waktu epitelisasi serta kerapatan serabut kolagen. Hasil penelitian dari persentase penyembuhan luka dan waktu epitelisasi yang dianalisa dengan uji General Linear Model Repeated Measures memberikan perbedaan secara nyata (p<0,05), sedangkan untuk pengamatan serabut kolagen menunjukkan tidak ada perbedaan secara nyata (p>0,05) dan untuk waktu epitelisasi yang diuji menggunakan uji T Independent Sample memberikan perbedaan secara nyata (p<0,05), maka dapat disimpulkan bahwa ekstrak terpurifikasi dapat memberikan efek dalam penyembuhan luka eksisi pada mencit.   Alfa mangosteen is a major compound of mangosteen extract cortex and  hasvarious bioactivities, such as anti-oxidant, anti-inflammatory and anti-bacterial so that can be used as wound healing. This research aimed to evaluate the activity of Purified Mangosteen Pericarp Fruit Cortex which contained alfa mangosteen > 90% as excision wound healing  agent in male mice by in vivo study. The parameters observed were Excision wound healing, epithelialization time and collagen fiber density. Mice were divided into two groups, group I was the negative control group (-) which was only received 1% Na CMC suspension, while group II was the treatment group received 1 % extract suspension.  On the 5th, 10th and 15th days the percentage of wound healing was measured and the epithelialization time and density of collagen fibers was observed.The results of the percentage of wound healing and epithelialization time evaluation which were analyzed by the General Linear Repeated Measures test showed a significant differences (p <0.05), whereas observation of collagen fibers showed no significant differences (p> 0.05) and analysis of epithelialization time using Independent Sample T test showed a significant difference (p <0.05), it can be concluded that purified extract has excision wounds healing effect in mice.

scholarly journals Chitosan-based biomaterials used in critical-size bone defects: radiographic study in rat's calvaria

2012 ◽  
Vol 41 (5) ◽  
pp. 312-317 ◽  
Author(s):  
Rubens Spin-Neto ◽  
Felipe Leite Coletti ◽  
Rubens Moreno de Freitas ◽  
Chaíne Pavone ◽  
Sérgio Paulo Campana-Filho ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Bone Density ◽  
High Molecular Weight ◽  
Critical Size ◽  
Bone Defects ◽  
Control Group ◽  
Low Molecular Weight ◽  
Chitosan Hydrochloride ◽  
Low Molecular Weight Chitosan ◽  
High Molecular Weight Chitosan

OBJECTIVE: This study evaluated, using digital radiographic images, the action of chitosan and chitosan hydrochloride biomaterials, with both low and high molecular weight, used in the correction of critical-size bone defects (CSBD's) in rat's calvaria. MATERIAL AND METHOD: CSBD's with 8 mm in diameter were surgically created in the calvaria of 50 Holtzman rats and these were filled with a blood clot (Control), low molecular weight chitosan, high molecular weight chitosan, low molecular weight chitosan hydrochloride and high molecular weight chitosan hydrochloride, for a total of 10 animals, which were divided into two experimental periods (15 and 60 days), for each biomaterial. The radiographic evaluation was made using two digital radiographs of the animal's skull: one taken right after the bone defect was created and the other at the moment of the sacrifice, providing the initial and the final radiographic bone density in the area of the defect, which were compared. RESULT: Analysis of radiographic bone density indicated that the increase in the radiographic bone density of the CSBD's treated with the proposed biomaterials, in either molecular weight, in both observed periods, where similar to those found in control group. CONCLUSION: Tested chitosan-based biomaterials were not able to enhance the radiographic density in the CSBD's made in rat's calvaria.

scholarly journals The Effects of Aloe vera Cream on the Expression of CD4+ and CD8+ Lymphocytes in Skin Wound Healing

2018 ◽  
Vol 2018 ◽  
pp. 1-5 ◽  
Author(s):  
Yos Adi Prakoso ◽  
Kurniasih
Keyword(s):  
Wound Healing ◽  
Topical Application ◽  
Aloe Vera ◽  
Skin Wound ◽  
Control Group ◽  
Histopathological Study ◽  
Sprague Dawley ◽  
Skin Wound Healing ◽  
Group I ◽  
Significant Difference

The aim of this study is to explore the effect of topical application of Aloe vera on skin wound healing. Thirty-six male Sprague-Dawley rats weighing 150–200 grams were divided into four groups. All groups were anesthetized, shaved, and exposed to round full-thickness punch biopsy on the back: group I (control); group II (treated with 1% Aloe vera cream); group III (treated with 2% Aloe vera cream); and group IV (treated with madecassol®). The treatments were given once a day. Macroscopic and microscopic examination were observed at 5, 10, and 15 days after skin biopsy. Skin specimens were prepared for histopathological study using H&E stain and IHC stain against CD4+ and CD8+ lymphocytes. All the data were analyzed using SPSS16. The result showed that topical application of 1% and 2% Aloe vera cream significantly reduced the percentage of the wound, leucocytes infiltration, angiogenesis, and expression of CD8+ lymphocytes and increased the epidermal thickness and the expression of CD4+ lymphocytes (p ≤ 0,05). There was no significant difference in the number of fibroblasts in all groups. Topical application of 1% and 2% Aloe vera cream has wound healing potential via their ability to increase the ratio of CD4+/CD8+ lymphocytes in the wound area.

scholarly journals Comparison of effects of unfractionated heparin and low molecular weight heparin on skin wound healing of rats

2012 ◽  
Vol 27 (9) ◽  
pp. 639-644 ◽  
Author(s):  
Can Engin Durmaz ◽  
Aydin Ozkan ◽  
Bugra Senel ◽  
Hasan Alper Uyar
Keyword(s):  
Molecular Weight ◽  
Wound Healing ◽  
Unfractionated Heparin ◽  
Low Molecular Weight Heparin ◽  
Skin Wound ◽  
Control Group ◽  
Low Molecular Weight ◽  
Skin Wound Healing ◽  
Significant Difference ◽  
Experimental Group

PURPOSE: To compare the effects of unfractionated heparin (UH) and a low molecular weight heparin (LMWH) on skin wound healing of rats. METHODS: Forty eight male Sprague-Dawley rats underwent 8mm full thickness dorsal skin wounds and were randomly assigned to three equal groups. In experimental group A, heparin sodium was injected at a concentration of 1000U/kg. In experimental group B, enoxaparin was injected at a concentration of 1mg/kg. Physiologic saline (0.5ml) was administered to the control group. Injections were made subcutaneously, once daily, for seven days. At 7th and 10th days tissue samples were taken from all rats. Histologic examination of these tissues was made under light microscope and scored. RESULTS: Histological examination showed a significant difference between the 7th and 10th day groups in wound healing. It was observed that wound healing of LMWH injected group is better. This difference is statistically significant at 10th day. CONCLUSIONS: Daily administration of single doses of unfractionated heparin and a low molecular weight heparin improves wound healing positively. Low molecular weight heparin induces wound healing more than unfractionated heparin.

scholarly journals PENGARUH PEMBERIAN GEL KOMBINASI EKSTRAK GETAH PEPAYA (Carica papaya L) DAN EKSTRAK DAGING LIDAH BUAYA (Aloe vera) TERHADAP PROSES PENYEMBUHAN ULKUS TRAUMATIKUS PADA MALE WISTAR RATS YANG MENDERITA DIABETES MELITUS

2015 ◽  
Vol 2 (1) ◽  
pp. 41
Author(s):  
Umi Kulsum ◽  
Ratnawati Hendari ◽  
Siti Chumaeroh
Keyword(s):  
Wound Healing ◽  
Ulcer Healing ◽  
Wistar Rats ◽  
Povidone Iodine ◽  
Healing Process ◽  
Aloe Vera ◽  
Control Group ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Papaya Latex

Introduction: Research on the use of papaya and aloevera sap on wound healing each been done, but effects the combination of the two extracts on wound healing in diabetes mellitus condition has not been done.Purpose: This study aims to determine the effect of a gel combination of papaya latex and aloevera flesh extracts on the traumatics ulcer healing process in diabetic rats.Methods: True experimental laboratory research with the post-test-only control group design that performed on 28 male wistar rats which were divided intofour groups: DM and non-DM rat that treatment with povidone iodine and gel combination of papaya latex and aloevera flesh extract. The treatment was done for 7 days with smearing that was given 2x/day. Traumatics ulcer healing seen from the number of fibroblasts that then were analyzed by One WayAnova and post hoc LSD test.Results: The results showed the number of fibroblasts in DM and non-DM rat with povidone iodine were 44.6 and 42.73; in the DM and non-DM rat with a gel combination of papaya latex and aloe vera flesh extracts were 61.10 and 77.03. One Way Anova test resulted p-value = 0.000, it was mean that there were at least a significant difference on the number of fibroblasts in the two groups. Differences of the number fibroblasts was shown in all groups, except in the group DM and non-DM rats with povidone iodine (p = 0.764).Conclusion: Conclusion of the study was the provision of a gel combination of papaya latex and aloevera flesh extracts effect on the traumatics ulcer healing process in diabetic rats.

scholarly journals The Effect of Aloe Vera Extract to Extensive Lesion and Expression of Transforming Growth Factor (TGF-β) on Alkaline Chemical Trauma Cornea Model

2018 ◽  
Vol 1 (2) ◽  
pp. 1-17
Author(s):  
Fahcreza ◽  
Elsa Iskandar ◽  
Rachmat Hidayat ◽  
Petty Purwanita ◽  
Anang Tribowo ◽  
...  
Keyword(s):  
Wound Healing ◽  
Growth Factor ◽  
Wistar Rats ◽  
Aloe Vera ◽  
Control Group ◽  
Treatment Groups ◽  
White Rats ◽  
Significant Difference ◽  
The Difference ◽  
In Cells

Abstract Background: Chemical trauma to the cornea is an emergency condition of the eye that requires early diagnosis and good treatment. Alkaline have ability to saponify fatty acids in cells and cell membranes which can make penetration into the stroma and destroy proteoglycans and collagen in cells. Aloe vera (AV) contains several active substances that are reported to have anti-inflammatory, immunomodulatory, and wound healing effects. AV has been reported to accelerate the healing process of corneal epithelial defects by increasing fibroblast proliferation, collagen production and growth factor production. This study aims to determine the difference between the effect of aloe vera extract with a concentration of 10%, 20%, 40% and BSS on the healing of extensive corneal lesions in white wistar rats alkaline trauma models. Method: This study was an experimental study with a pre and posttest only with control group design in vivo approach to 30 Wistar white rats which were divided into 5 treatment groups for 3 days. Comparative analysis of effectiveness using the ANNOVA test or the Kruskal Wallis test and continued by the post hoc test. Results: Based on the one way ANOVA test there was a statistically significant difference in effectiveness between the five treatment groups on the percentage of corneal wound healing area and TGF-β expression with an assessment of p = 0,000 each. The administration of alloevera (AV) concentration of 20% had a significant difference in percentage of healing of corneal lesions and TGF-β expression compared with other treatment groups with p = 0,000 each. Large differences in the area of corneal lesions in the 40% AV group were -0.45 in the BBS group, 0.146 in the 10% AV group, 0.493 in the 20% AV group. The difference in the AV group 10% was 0.30 in the BBS group, -064 in the AV group 20%, and -0.14 in the AV group 40%. However, TGFβ expression in the normal control group that did not receive treatment was 54.94 (53.21-56-12). TGFβ levels in the BSS group were 10.44, the 10% aloe vera group was 25.43, 47.99 for the 20% aloe vera group and 37.95 for the 40% aloe vera group. Conclusion: There is a difference between the effect of aloe vera extract with concentrations of 10%, 20%, 40% and BSS on the extensive healing of corneal lesions in white wistar rats with alkaline chemical trauma models.

Searching for synergistic effects of low-molecular weight chitosan derivatives, chitosan and copper nanoparticles for wound healing ointment

2021 ◽  
Vol 12 (3) ◽  
pp. 035016
Author(s):  
Natalya Nikolaevna Glushchenko ◽  
Olga Alexandrovna Bogoslovskaya ◽  
Balzhima Tsyrendorzhievna Shagdarova ◽  
Alla Victorovna Il’ina ◽  
Irina Pavlovna Olkhovskaya ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Wound Healing ◽  
Copper Nanoparticles ◽  
Synergistic Effects ◽  
Low Molecular Weight ◽  
Chitosan Derivatives ◽  
Low Molecular Weight Chitosan

scholarly journals The effect of Aloe Vera leaf gel in promoting wound healing and as antibacterial

2008 ◽  
Vol 32 (2) ◽  
pp. 46-59
Author(s):  
Ali Aziz Al-Khayyat
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Wound Healing ◽  
Pathogenic Bacteria ◽  
Nigella Sativa ◽  
Aloe Vera ◽  
Self Control ◽  
Control Group ◽  
Nigella Sativa Oil ◽  
Normal Wound Healing ◽  
Significant Difference

This study was carried out to explore the effect of Aloe Vera leafgel in promoting wound healing and to investigate theantibacterial effect against some pathogenic bacteria in comparisonwith Nigella sativa oil. Standard dilutions of Aloe Vera leaf gel weremade from ten to one hundred percent and its antibacterial effecthad been examined in seeded agar method against Staphylococcusaureus and Pseudomonas aeruginosa both were previously identifiedby laboratory and biochemical methods, Nigella Sativa oil which isknown to be one of the important medicinal plant was used forcomparison . Statistical analysis showed a significant difference(P<0.05) between AVG and Nigella Sativa oil.The effect of Aloe Vera leaf gel against Staph .aureus was morepotent than against Pseudomonas aeruginosa. There was aproportional relation between different concentrations of AVGwith the values of inhibition zones diameters of the bacteria.Results showed that Aloe Vera was more potent than Nigella sativaagainst both bacteria.The Minimum Inhibitory Concentration (MIC) was sixtymg/ml and the Minimum Bactericidal Concentration (MBC) waseighty mg/ml for Staph .aureus.In order to investigate the effect of Aloe Vera gel on woundhealing, twenty-four local male rabbits were used. They weredivided into four equal groups and each animal was wounded inboth sides of the back region by making a one by two centimetersquare standard longitudinal incision with surgical scalpel. Thefirst group was a control group (wounded without treatment); thisgroup was employed to observe the normal wound healing. Thesecond group was treated with crude Aloe Vera leaf gel twice dailyfor 10 days. While the third group was wounded and infected withthe pathogenic bacteria Staphylococcus vaureus without treatment,in order to observe the natural body defense against pathogenicmicroorganisms. The last group was infected with the samebacteria but treated with crude Aloe Vera leaf gel to observe theantibacterial effect. The wounds in the left side in second andfourth group were left as self-control.

scholarly journals Antiadhesive Property of Photoreactive Azidophenyl Low-Molecular-Weight Chitosan in Rabbit Laminotomy Model

2013 ◽  
Vol 2013 ◽  
pp. 1-8
Author(s):  
Jong Won Kim ◽  
Kwang-Sup Song ◽  
Hyun Kang ◽  
Eui-Chan Jang ◽  
Mi Kyung Kim ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Hyaluronic Acid ◽  
Inflammatory Cells ◽  
Saline Group ◽  
Low Molecular Weight ◽  
Group Iii ◽  
Group I ◽  
Low Molecular Weight Chitosan ◽  
Peridural Fibrosis ◽  
Mechanical Barrier

Newly developed photoreactive azidophenyl chitosan (P-ALMC) has characteristics of a transformable gel type and its outer layer could be sealed up like a film after UV radiation. We aim to evaluate the antiadhesive properties of P-ALMC through comparing it with hyaluronic acid-carboxymethylcellulose membrane (HA-CMC) in a rabbit laminotomy model. Laminotomies were performed at the L3-4, L4-5, and L5-6 levels in 41 rabbits and each level was randomly assigned to either receive saline (group I), HA-CMC (group II), or P-ALMC (group III). The extent of peridural fibrosis, density of fibroblasts and inflammatory cells, and dural thickness were evaluated at 6 and 12 weeks postoperatively. In the groups II and III, the extents of peridural fibrosis and dural thickness were significantly smaller than those in group I (P<0.001) and no differences between groups II and III were found at the postoperative 6 and 12 weeks. There were no differences of cell density among groups. P-ALMC showed effective antiadhesive properties comparable to HA-CMC and could be one of the candidates as an anti-adhesive agent for spine surgery even further study is required to identify the effectiveness of its unique characteristics as mechanical barrier.

scholarly journals Reproductive and Behavioral Evaluation of a New Immunocastration Dog Vaccine

Animals ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 226
Author(s):  
Daniela Siel ◽  
María José Ubilla ◽  
Sonia Vidal ◽  
Alexandra Loaiza ◽  
John Quiroga ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Recombinant Protein ◽  
Control Group ◽  
Low Molecular Weight ◽  
Specific Antibodies ◽  
Controlled Conditions ◽  
Low Molecular Weight Chitosan ◽  
And Control ◽  
Complementary Strategy ◽  
Positive Effect

Canine immunocastration development has been of interest for many years as a complementary strategy to surgical castration. The purpose of this paper was to verify the effect of a recombinant vaccine for dog immunocastration. Two tests were done, one under controlled conditions and a second under field conditions. Animals were injected with 1 mL of 500 µg GnRXG/Q recombinant protein; 500 µg of low molecular weight chitosan as adjuvant; 1 mL NaCl 0.9% q.s. In the first trial, eight Beagle male dogs between the ages of 1 and 3 comprised the sample, randomly divided into two groups: vaccinated group (n = 7) and control group (n = 2). The second trial had 32 dogs with owners. In the first controlled conditions trial, the vaccine produced specific antibodies that remained until the end of the trial (day 270), inducing reduced testosterone and spermiogram changes in the immunized animals. In a second trial, on the field, specific immunity was induced, which remained high up to day 150. The vaccine also reduced sexual agonistic and marking behaviors. This new vaccine proved to be safe, immunogenic, capable of reducing gonadal functionality, and had a positive effect on inducing reduced sexual, agonistic, and marking behavior of the animals.

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