scholarly journals Determination of Total Flavonoids Contents and Antioxidant Activity of Ginkgo biloba Leaf by Near-Infrared Reflectance Method

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Ling-jia Zhao ◽  
Wei Liu ◽  
Su-hui Xiong ◽  
Jie Tang ◽  
Zhao-huan Lou ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Quality Control ◽  
Quantitative Analysis ◽  
Ginkgo Biloba ◽  
Near Infrared ◽  
Total Flavonoids ◽  
Total Antioxidant Activity ◽  
Near Infrared Reflectance ◽  
Total Antioxidant ◽  
Mean Square Errors

Background. Total flavonoids content (TFC) is one of the most important quality indexes of Ginkgo biloba leaf, and it is concerned with total antioxidant activity. Near-infrared spectroscopy (NIR) method has showed its advantages in fast, accurate, qualitative, and quantitative analysis of various components in many quality control researches. In this study, a calibration model was built by partial least squares regression (PLSR) coupling with NIR spectrum to quantitatively analyze the TFC and total antioxidant activity of Ginkgo biloba leaf. Results. During the model establishing, some spectrum pretreatment and outlier diagnosis methods were optimized to establish the final model. The coefficients of determination (R2) for TFC and total antioxidant activity prediction were 0.8863 and 0.8486, respectively; and the root mean square errors of prediction (RMSEP) were 2.203 mg/g and 0.2211 mM/g, respectively. Conclusion. These results showed that NIR method combined with chemometrics is suitable for quantitative analysis of main components and their activities and might be applied to quality control of relevant products.

scholarly journals Effect of post harvest ripening on bioactive secondary metabolites and antioxidant activity in mango cv. Amrapali

2018 ◽  
Vol 13 (2) ◽  
pp. 152-158
Author(s):  
B M Muralidhara ◽  
G L Veena ◽  
S Rajan ◽  
A K Bhattacherjee ◽  
Pavan Kumar Malav
Keyword(s):  
Antioxidant Activity ◽  
Total Flavonoids ◽  
Total Antioxidant Activity ◽  
Total Phenols ◽  
Fruit Peel ◽  
Total Carotenoids ◽  
Post Harvest ◽  
Total Antioxidant ◽  
Mango Peel ◽  
Ripe Stage

Mango possesses many bioactive phytonutrients at ripe stage which boost our immune systemagainst many diseases. Post harvest ripening plays a major role in changes in those bioactivephytochemicals and their antioxidant activity. Hence, the present study was undertaken toassess the changes in bioactive phytonutrients and total antioxidant activity during ripeningof mango cv. Amrapali. The fruits were analyzed for total antioxidants, total phenols, totalflavonoids and total carotenoids from the day of harvest to its deterioration. Fruit peel andpulp color was measured with SPH850 spectrophotometer on the basis of the CIE LAB colorsystem (L*, a* and b*). The results revealed that total phenols (36.11 to 66.53mg GAE 100g-1),total flavonoids (14.33 to 34.67mg QE 100g-1), total carotenoids (2.23 to 11.47mg 100g-1) andtotal antioxidant (0.37 to 0.76 mmol Trolox 100g-1) activity increased gradually from day one toninth day after harvest and decreased slightly thereafter up to eleventh day of harvest excepttotal carotenoids, which remained constant. Strong correlations between total phenols (0.94),total flavonoids (0.86) and total carotenoids (0.97) with total antioxidant activity were noticed.Positive relationship between total carotenoids and L*, a*, b* values in mango peel and pulpduring ripening was also observed. It can be concluded that ripening affected the compositionof bioactive phytonutrients and their antioxidant activity in mango andmaximum nutraceuticalscontents were noticed from seven to nine days after harvest.

scholarly journals Estimation of flavonoids and screening of in vitro antioxidant activity of various extracts of aerial parts of Blepharis maderaspatensis by hydroxyl radical and total antioxidant activity

2020 ◽  
Vol 11 (SPL4) ◽  
pp. 1204-1209
Author(s):  
Jambula Dinesh Babu ◽  
Bhuvaneswari Santharam ◽  
Kottai Muthu Arumugam
Keyword(s):  
Antioxidant Activity ◽  
Hydroxyl Radical ◽  
Bioactive Compound ◽  
Antioxidant Potential ◽  
Total Flavonoids ◽  
Total Antioxidant Activity ◽  
Aerial Parts ◽  
Total Antioxidant ◽  
Total Flavonoids Content

Antioxidants are substances that hamper or inhibit the oxidation of oxidizable substrates in the cells. The aerial parts of Blepharis maderaspatensis dry powder was extracted with various solvents (PE, EA and methanol) through Soxhlet extractor. The aerial parts of different concentrates (Pet.ether, ethyl acetate and methanol) of Blepharis maderaspatensis was evaluated for its in-vitro antioxidant potential by hydroxyl radical, total antioxidant activity taking ascorbate used as standard for the both methods and total flavonoids content was estimated as equivalent to rutin. The methanolic concentrates of Blepharis maderaspatensis & standard exhibited antioxidant potential possessing IC50 210µg/ml & 62µg/ml (hydroxyl radical) 200µg/ml & 57µg/ml (Total antioxidant activity) respectively. An IC50 value was originate that methanolic concentrates of Blepharis maderaspatensis more efficient in hydroxyl radical, total antioxidant activity compared EA & PE concentrates. The methanolic and EA concentrates of Blepharis maderaspatensis showed the total flavonoids content (11.230±0.22 and 2.858±0.56 mg of rutin equivalent/g of powder) respectively. The difference in scavenging potential of the extracts can be due to variation in the percentage of bioactive compound flavonoids present in methanolic extracts. Invitro antioxidant studies obviously show methanolic concentrates of Blepharis maderaspatensis have better antioxidant activity due to the presence of total flavonoids content. The findings from the study reveal that the antioxidative potentials of methanolic concentrates Blepharis maderaspatensis could serve as free radical inhibitors, possibly acting as natural antioxidants.

Diurnal changes in the total antioxidant activity of human saliva

2007 ◽  
Vol 33 (3) ◽  
pp. 375-376 ◽  
Author(s):  
M. F. Borisenkov ◽  
L. A. Erunova ◽  
E. M. Lyuseva ◽  
N. V. Pozdeeva
Keyword(s):  
Antioxidant Activity ◽  
Human Saliva ◽  
Diurnal Changes ◽  
Total Antioxidant Activity ◽  
Total Antioxidant

scholarly journals Biochemical study of hepatoprotective collection of plant origin

2019 ◽  
Vol 59 (7) ◽  
pp. 134-141
Author(s):  
Anatoly A. Lapin ◽  
◽  
Irek G. Garifullin ◽  
Valery N. Zelenkov ◽  
◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Liver Diseases ◽  
Biochemical Study ◽  
Water Extract ◽  
Total Antioxidant Activity ◽  
Plant Origin ◽  
Treatment And Prevention ◽  
Total Antioxidant ◽  
The Individual ◽  
Metabolic Regulators

The article presents the results of biochemical studies of the total antioxidant activity of the hepatoprotective collection of plant origin and its individual components, which show that it consists of birch leaves (Betulae folia), tansy flowers (Tanaceti flores), phytohepatol No. 2 (choleretic collection No. 2) and individual its components have pronounced antioxidant activity. More antioxidant activity founded in tansy and true hepatoprotective collection. Comparative tests of the flowers of meadowsweet, with the highest antioxidant activity, showed the promise of their use in the collections of medicinal plants as effective natural metabolic regulators. The total antioxidant activity of the water extract collection at 13.56% rel. exceeded the calculated value obtained by summing the activities of the individual components of the collection, which indicates the presence of synergism in their action, which may be of value for use in the treatment and prevention of liver diseases. When all components of the drug collection are finished, the indicator increases by 11-24 % rel., which indicates the thermal stability of the antioxidant substances that make up their composition. In the oxidation of aqueous extracts of the components of hepatoprotective collection with hydrogen peroxide, the most stable were antioxidants of birch leaves. It was found that collection only at a ratio of 7.7 g per liter of water shows synergism (15 % rel.), and the rest of all the samples we tested are antagonistic. The detected increase in the antioxidant activity of the new collection compared with the effects of its individual components allows us to consider it as a promising dosage form for use in the treatment and prevention of liver diseases.

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