Therapeutic Effect of Xuebijing, a Traditional Chinese Medicine Injection, on Rheumatoid Arthritis

Background. Traditional Chinese medicine considers that rheumatoid arthritis (RA) is caused by blood stasis, heat, and toxins. Xuebijing (XBJ), a traditional Chinese medicine compound injection, activates blood circulation to dissipate blood stasis, eliminating pathogenic heat from the blood and degrading toxins. XBJ was approved by the China FDA to treat for many years. This study examined the potential therapeutic effects of XBJ on RA and rat collagen-induced arthritis (CIA). Methods. XBJ was cultured with the synovial fluid (SF) of RA patients. XBJ was also injected into CIA rats. Changes in Treg and Th17 cell levels in the peripheral blood (PB), SF, and spleen and changes in Th1/Th2 and cytokine levels in PB were detected using flow cytometry. Four RA patients were treated using XBJ based on Chinese medical theory and Chinese medicine indications. Results. Following culture with XBJ, the proportion of Treg cells (P=0.007) was significantly increased in RA SF, while the Th1/Th2 ratio remained unchanged. After XBJ treatment, CIA in rats was significantly relieved (P<0.001). The Treg cell proportion was significantly increased in the PB, SF, and spleen in the treated rats, while the number of Th17 cells decreased. The ratio of Th1/Th2 remained unchanged in PB, and the levels of IL-1β, IL-6, IL-17A, IFN-γ, and TNF-α decreased (P=0.031, P<0.001, P<0.001, P<0.001, and P=0.001, respectively). After XBJ treatment, the disease activity score-28 (DAS28), erythrocyte sedimentation rate (ESR), C-reactive protein (CRP) level, Th17 cell number, and Th1/Th2 cell ratio in the four RA patients were significantly decreased, while the Treg cell proportion was increased. Conclusion. XBJ can restore the immune balance to treat RA and CIA. Therefore, XBJ could be a potential therapeutic drug for RA.

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High Levels of CD38+ NK Cells Contribute to Immune Imbalance of Th1/Th2 and Th17/Treg in Rheumatoid Arthritis

10.21203/rs.2.20626/v1 ◽

2020 ◽

Author(s):

Hongxing Wang ◽

Kehua Fang ◽

Xiaotian Chang

Keyword(s):

Rheumatoid Arthritis ◽

Flow Cytometry ◽

T Cells ◽

Synovial Fluid ◽

Nk Cells ◽

Treg Cell ◽

Cd4 T Cells ◽

Th17 Cell ◽

Ifn Γ ◽

Cell Proportion

Abstract Background Increased CD38 expression and CD38+ cell proportion as well as their importance had been reported in rheumatoid arthritis (RA).Methods The proportion of lymphocyte subtypes in RA patients and rats with collagen-induced arthritis (CIA) was examined using flow cytometry. CD38+ NK cells, CD38+ NKT cells and CD4+ T cells as well as mononuclear cells (MNCs) depleted of CD38+ cells were isolated from RA synovial fluid using flow cytometry and cocultured in transwell apparatus.Results This study detected a significantly increased CD38+ NK cell proportion and a decreased CD38+ NKT cell proportion in RA peripheral blood and synovial fluid. The CD38+ NK/CD38+ NKT ratio was positively correlated with the disease activity. A similar result was observed in CIA rats. When CD38+ NK cells were cocultured with MNCs, the Treg cell proportion in MNCs and IL-10 level significantly decreased, and Th17 cell proportion and IFN-γ level increased. When the CD38+ NK cells were pretreated with monoclonal anti-CD38 antibody, Treg cell proportion and IL-10 level significantly increased, and the Th17 cell proportion and IFN-γ and IL-6 level decreased. When CD38+ NK cells were cocultured with CD4+ T cells, the Th1/Th2 and Th17/Treg ratios significantly increased, and mTOR signaling was activated in the cells. When the CD38+ NK cells were pretreated with the anti-CD38 antibody, the opposite result was obtained. Coculturing CD38+ NKT cells with MNCs or CD4+T cells showed opposite results. The anti-CD38 antibody also significantly increased TGF-β expression in the CD38+ NK cells.Conclusions Our results suggest that a high CD38+ NK and low CD38+ NKT proportion in RA elevates Th1/Th2 and Th17/Treg ratio to contribute to the pathogenesis.

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Breast cancer pathogenesis of stagnation of phlegm, poison and blood stasis: rationale and clinical application in traditional in traditional Chinese medicine

Journal of Chinese Integrative Medicine ◽

10.3736/jcim20070203 ◽

2007 ◽

Vol 5 (2) ◽

pp. 122-125 ◽

Cited By ~ 4

Author(s):

Sheng Liu

Keyword(s):

Breast Cancer ◽

Chinese Medicine ◽

Traditional Chinese Medicine ◽

Clinical Application ◽

Blood Stasis ◽

Cancer Pathogenesis

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Metabolomics study of the therapeutic mechanism of a Chinese herbal formula on collagen-induced arthritis mice

RSC Advances ◽

10.1039/c8ra05528a ◽

2019 ◽

Vol 9 (7) ◽

pp. 3716-3725 ◽

Cited By ~ 1

Author(s):

Zhen Jin ◽

Ji-da Zhang ◽

Xin Wu ◽

Gang Cao

Keyword(s):

Rheumatoid Arthritis ◽

Chinese Medicine ◽

Traditional Chinese Medicine ◽

Herbal Formula ◽

Collagen Induced Arthritis ◽

Chinese Herbal Formula ◽

Chinese Herbal ◽

Therapeutic Mechanism ◽

Metabolomics Study

Wenjinghuoluo (WJHL) prescription, the typical rheumatoid arthritis (RA) treatment compound in traditional Chinese medicine, shows favorable efficacy.

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Treatment of Rheumatoid Arthritis with Traditional Chinese Medicine

BioMed Research International ◽

10.1155/2014/528018 ◽

2014 ◽

Vol 2014 ◽

pp. 1-11 ◽

Cited By ~ 8

Author(s):

Wen-Yuan Lee ◽

Hsin-Yi Chen ◽

Kuan-Chung Chen ◽

Calvin Yu-Chian Chen

Keyword(s):

Rheumatoid Arthritis ◽

Chinese Medicine ◽

Traditional Chinese Medicine ◽

Protein Complex ◽

Chronic Inflammatory Disease ◽

Dynamics Simulation ◽

Current Therapy ◽

Potential Candidate ◽

Interleukin 6 Receptor ◽

The Stability

Rheumatoid arthritis (RA) is a chronic inflammatory disease that will affect quality of life and, working efficiency, and produce negative thoughts for patients. Current therapy of RA is treated with disease-modifying antirheumatic drugs (DMARDs). Although most of these treatment methods are effective, most patients still have a pleasant experience either due to poor efficacy or side effects or both. Interleukin-6 receptor (IL6R) is important in the pathogenesis of RA. In this study, we would like to detect the potential candidates which inhibit IL6R against RA from traditional Chinese medicine (TCM). We use TCM compounds from the TCM Database@Taiwan for virtually screening the potential IL6R inhibitors. The TCM candidate compound, calycosin, has potent binding affinity with IL6R protein. The molecular dynamics simulation was employed to validate the stability of interaction in the protein complex with calycosin. The analysis indicates that protein complex with calycosin is more stable. In addition, calycosin is known to be one of the components ofAngelica sinensis, which has been indicated to have an important role in the treatment of rheumatoid arthritis. Therefore, calycosin is a potential candidate as lead compounds for further study in drug development process with IL6R protein against rheumatoid arthritis.

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Therapeutic Effects of Traditional Chinese Medicine on Spinal Cord Injury: A Promising Supplementary Treatment in Future

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2016/8958721 ◽

2016 ◽

Vol 2016 ◽

pp. 1-18 ◽

Cited By ~ 14

Author(s):

Qian Zhang ◽

Hao Yang ◽

Jing An ◽

Rui Zhang ◽

Bo Chen ◽

...

Keyword(s):

Spinal Cord ◽

Spinal Cord Injury ◽

Chinese Medicine ◽

Traditional Chinese Medicine ◽

Epigallocatechin Gallate ◽

Therapeutic Effects ◽

Cord Injury ◽

Supplementary Treatment ◽

Natural Drugs ◽

Doctoral Dissertations

Objective. Spinal cord injury (SCI) is a devastating neurological disorder caused by trauma. Pathophysiological events occurring after SCI include acute, subacute, and chronic phases, while complex mechanisms are comprised. As an abundant source of natural drugs, Traditional Chinese Medicine (TCM) attracts much attention in SCI treatment recently. Hence, this review provides an overview of pathophysiology of SCI and TCM application in its therapy.Methods. Information was collected from articles published in peer-reviewed journals via electronic search (PubMed, SciFinder, Google Scholar, Web of Science, and CNKI), as well as from master’s dissertations, doctoral dissertations, and Chinese Pharmacopoeia.Results. Both active ingredients and herbs could exert prevention and treatment against SCI, which is linked to antioxidant, anti-inflammatory, neuroprotective, or antiapoptosis effects. The detailed information of six active natural ingredients (i.e., curcumin, resveratrol, epigallocatechin gallate, ligustrazine, quercitrin, and puerarin) and five commonly used herbs (i.e., Danshen, Ginkgo, Ginseng, Notoginseng, and Astragali Radix) was elucidated and summarized.Conclusions. As an important supplementary treatment, TCM may provide benefits in repair of injured spinal cord. With a general consensus that future clinical approaches will be diversified and a combination of multiple strategies, TCM is likely to attract greater attention in SCI treatment.

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Clinical Therapeutic Effects of Aspirin in Combination with Fufang Danshen Diwan, a Traditional Chinese Medicine Formula, on Coronary Heart Disease: A Systematic Review and Meta-Analysis

Cellular Physiology and Biochemistry ◽

10.1159/000447892 ◽

2016 ◽

Vol 39 (5) ◽

pp. 1955-1963 ◽

Cited By ~ 16

Author(s):

Jianchun Huang ◽

Xiaojun Tang ◽

Fangxing Ye ◽

Junhui He ◽

Xiaolong Kong

Keyword(s):

Systematic Review ◽

Coronary Heart Disease ◽

Heart Disease ◽

Chinese Medicine ◽

Traditional Chinese Medicine ◽

Randomized Clinical Trials ◽

Meta Analysis ◽

Aspirin Therapy ◽

Therapeutic Effects ◽

Chinese Medicine Formula

Background/Aims: Coronary heart disease is characterized by vascular stenosis or occlusion resulting in myocardial ischemia, hypoxia and necrosis. In China, the combination of aspirin and Fufang Danshen Diwan (FDD), a traditional Chinese medicine formula, has been suggested in the treatment of coronary heart disease. There have been several studies comparing the effectiveness of aspirin alone and in combination with FDD to treat coronary artery disease; however, it remains unclear whether combined aspirin therapy is superior. This study was thus designed to clarify this issue through a systematic review and meta-analysis. Methods: Databases including PubMed, EMBASE, China National Knowledge Infrastructure (CNKI) database, Wanfang Data and VIP Information were searched. Papers were reviewed systematically by two researchers and analyzed using Cochrane software Revman 5.1. Results: Fourteen randomized controlled trials enrolling 1367 subjects were included. Meta-analyses revealed that aspirin in combination with FDD was significantly more effective at alleviating angina pectoris and improving electrocardiogram (ECG) results relative to aspirin therapy alone, reflected by the summary effects for the clinical markedly effective (OR = 2.45; 95% CI 1.95-3.08) and the total effective (OR = 3.92; 95% CI 2.87-5.36) rates. In addition, combined aspirin and FDD was significantly more efficacious than aspirin monotherapy at improving blood lipid levels, as indicated by the following outcomes: 1) reduction of TC level (SMD −1.12; 95% CI −1.49 to −0.76); 2) reduction of TG level (SMD −0.94; 95% CI −1.15 to -0.74); 3) reduction of LDL level (SMD -0.68; 95% CI -0.88 to -0.48); and 4) improvement of HDL level (SMD 0.52; 95% CI 0.04 to 0.99 ). No serious adverse events were reported in any of the included trials. Conclusion: The present meta-analysis demonstrated that aspirin in combination with FDD was more effective than aspirin alone for treating coronary heart disease. More full-scale randomized clinical trials with reliable designs are recommended to further evaluate the clinical benefits and long-term effectiveness of FDD for the treatment of coronary heart disease.

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A traditional Chinese medicine versus Western combination therapy in the treatment of rheumatoid arthritis: two-stage study protocol for a randomized controlled trial

Trials ◽

10.1186/1745-6215-12-137 ◽

2011 ◽

Vol 12 (1) ◽

Cited By ~ 15

Author(s):

Chi Zhang ◽

Miao Jiang ◽

Aiping Lu

Keyword(s):

Rheumatoid Arthritis ◽

Randomized Controlled Trial ◽

Chinese Medicine ◽

Traditional Chinese Medicine ◽

Combination Therapy ◽

Study Protocol ◽

Controlled Trial ◽

Two Stage ◽

Randomized Controlled

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Molecular Imaging in Traditional Chinese Medicine Therapy for Neurological Diseases

BioMed Research International ◽

10.1155/2013/608430 ◽

2013 ◽

Vol 2013 ◽

pp. 1-11 ◽

Cited By ~ 15

Author(s):

Zefeng Wang ◽

Haitong Wan ◽

Jinhui Li ◽

Hong Zhang ◽

Mei Tian

Keyword(s):

Chinese Medicine ◽

Molecular Imaging ◽

Traditional Chinese Medicine ◽

Neurological Disorders ◽

Neurological Diseases ◽

Chinese Herb ◽

Public Health Care ◽

Therapeutic Effects ◽

Cord Injury

With the speeding tendency of aging society, human neurological disorders have posed an ever increasing threat to public health care. Human neurological diseases include ischemic brain injury, Alzheimer’s disease, Parkinson’s disease, and spinal cord injury, which are induced by impairment or specific degeneration of different types of neurons in central nervous system. Currently, there are no more effective treatments against these diseases. Traditional Chinese medicine (TCM) is focused on, which can provide new strategies for the therapy in neurological disorders. TCM, including Chinese herb medicine, acupuncture, and other nonmedication therapies, has its unique therapies in treating neurological diseases. In order to improve the treatment of these disorders by optimizing strategies using TCM and evaluate the therapeutic effects, we have summarized molecular imaging, a new promising technology, to assess noninvasively disease specific in cellular and molecular levels of living models in vivo, that was applied in TCM therapy for neurological diseases. In this review, we mainly focus on applying diverse molecular imaging methodologies in different TCM therapies and monitoring neurological disease, and unveiling the mysteries of TCM.

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