scholarly journals Multimodal α-Glucosidase and α-Amylase Inhibition and Antioxidant Effect of the Aqueous and Methanol Extracts from the Trunk Bark of Ceiba pentandra

2020 ◽  
Vol 2020 ◽  
pp. 1-13
Author(s):  
Telesphore Benoit Nguelefack ◽  
Christian Kuete Fofie ◽  
Elvine Pami Nguelefack-Mbuyo ◽  
Adeline Kaptue Wuyt
Keyword(s):  
Protein Oxidation ◽  
Postprandial Hyperglycemia ◽  
Alpha Amylase ◽  
Total Phenolic ◽  
Noncompetitive Inhibition ◽  
Ceiba Pentandra ◽  
Flavonoid Compounds ◽  
Alpha Glucosidase ◽  
Phenolic And Flavonoid ◽  
Phenolic And Flavonoid Compounds

Postprandial hyperglycemia and oxidative stress are important factors that worsen the health condition of patients with type 2 diabetes. We recently showed that extracts from Ceiba pentandra mitigate hyperglycemia in dexamethasone- and high diet/streptozotocin-induced diabetes. Herein, we evaluated the postprandial regulatory properties and the antioxidant effects of the aqueous (AE) and methanol (ME) extracts from the stem bark of Ceiba pentandra. The phytochemical analysis of AE and ME was performed using the LC-MS technique and the total phenolic and flavonoid assays. Both extracts were tested for their ability to inhibit superoxide anion (O2•ـ), hydrogen peroxide (H2O2), protein oxidation, alpha-amylase, and alpha-glucosidase activities. The mode of enzyme inhibition was also determined in a kinetic study. AE and ME were both rich in phenolic and flavonoid compounds. ME was 2.13 and 1.91 times more concentrated than AE in phenolic and flavonoid compounds, respectively. LC-MS allowed the identification of 5 compounds in both extracts. ME and AE inhibited O2•ـ with IC50 of 51.81 and 34.26 μg/ml, respectively. On H2O2, they exhibited IC50 of 44.84 and 1.78 μg/ml, respectively. Finally, they exhibited IC50 of 120.60 and 140.40 μg/ml, respectively, in the inhibition of protein oxidation induced by H2O2, while showing IC50 of 39.26 and 97.95 μg/ml on the protein oxidation induced by AAPH. ME and AE inhibited alpha-amylase with IC50 of 6.15 and 54.52 μg/ml, respectively. These extracts also inhibited alpha-glucosidase, demonstrating IC50 of 76.61 and 86.49 μg/ml. AE exhibited a mixed noncompetitive inhibition on both enzymes, whereas ME exhibited a competitive inhibition on α-amylase and a pure noncompetitive inhibition on α-glucosidase. These results demonstrate that ME and AE scavenge reactive oxygen species and prevent their effects on biomolecules. Besides, ME and AE inhibit carbohydrate digestive enzymes. These properties may contribute to reduce postprandial hyperglycemia and regulate glycemia in diabetic patients.

scholarly journals Optimization of Extraction of Total Phenolic and Total Flavonoid from Seeds of Alpinia blepharocalyx K. Schum. Using The Response Surface Methodology

2019 ◽  
Vol 57 (3B) ◽  
pp. 105
Author(s):  
Thanh Tan Nguyen ◽  
Huyen Thi Thanh Mai ◽  
Tuan Ngoc Nguyen ◽  
Quang Dinh Tran ◽  
Thang Dinh Tran
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Antioxidant Properties ◽  
Total Flavonoid ◽  
Extraction Time ◽  
Total Phenolic ◽  
Extraction Temperature ◽  
Flavonoid Compounds ◽  
Phenolic And Flavonoid ◽  
Phenolic And Flavonoid Compounds

Seeds of Alpinia blepharocalyx contain high amounts of phenolic and flavonoid compounds with potential antioxidant properties. In this study, the effects of the extraction method on the recovery of phenolic and flavonoid compounds from Alpinia blepharocalyx seeds were investigated. Response surface methodology (RSM) has been used to optimize the extraction conditions of total phenolic and total flavonoid from seeds of Alpinia blepharocalyx. A Box-Behnken design was used to investigate the effects of four independent variables, namely extraction temperature (°C), extraction time (min), (v/m) and ethanol concentration (%) on the responses: total phenolic content (TPC) and yield. The optimal conditions obtained from response RSM were 52.66 % v/v for the solvent composition, 62.34 (°C) for extraction temperature, 34.48/1 (ml/g) for solvent/material ratio and 125.42 (min) for extraction time. The experimental values of TPC, TFC and yield were 39.31±0.05 mgGAE/g, 12.75±0.07 mgCE/g and 6.97±0.05%, respectively.   

Effect of gamma irradiation processing on total phenol and antioxidant capacities of the Iranian extract of propolis

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Marzieh Heidarieh ◽  
Ali Nabipour Chakoli ◽  
Samira Shahbazi ◽  
Parvin Shawrang ◽  
Baode Zhang
Keyword(s):  
Gamma Irradiation ◽  
Antioxidant Activities ◽  
Reducing Power ◽  
The Other ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Flavonoid Compounds ◽  
Gamma Irradiated ◽  
Phenolic And Flavonoid ◽  
Phenolic And Flavonoid Compounds

Abstract The Iranian ethalonic extract of propolis (EEP) were analyzed to determine their antioxidant activities and total phenolic and flavonoid contents, with and without gamma irradiation processing under various dosage of gamma (10, 20, 30, 40 and 50 kGy). The analysis results show that the gamma irradiated EEP at 10 KGy can scavenge free radicals and exhibit higher antioxidant-reducing power to the other groups (P < 0.05). It is noted that gamma irradiated EEP has higher total phenolic contents compared to gamma irradiated EEP at 30, 40 and 50 KGy and non-irradiated EEP (P < 0.05). The flavonoid compounds as indicated by C-13 NMR analysis were found to be higher in gamma irradiated EEP at 10 kGy compared with the other groups. In conclusion, gamma irradiation processing of EEP causes can enhanced antioxidant activities phenolic and flavonoid compounds at dose of 10 KGy.

scholarly journals Study of Potential Phenolic Compounds from Stems of Dendrophthoe Falcata (Loranthaceae) Plant as Antioxidant and Antimicrobial Agents

2018 ◽  
Vol 34 (5) ◽  
pp. 2342-2349 ◽  
Author(s):  
Sri Atun ◽  
Sri Handayani ◽  
Anna Rakhmawati ◽  
Nur Aini Purnamaningsih ◽  
Bian Ihda An Naila ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Pathogenic Bacteria ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Flavonoid Compounds ◽  
Dpph Method ◽  
Phenolic And Flavonoid ◽  
Phenolic And Flavonoid Compounds ◽  
Dendrophthoe Falcata

Dendrophthoe falcata is a hemiparasitic plant that has been used in traditional medicine.The study was conducted to identify the total phenolic and flavonoid compounds, to test antioxidant, and antimicrobial activity of ethanol extract stems of D. falcata parasite on Melia azedarach host tree. The dry powder stems of D. falcata was extracted with ethanol. The ethanol extract was subsequently partitioned successively using n-hexane, chloroform, and ethyl acetate. Each fraction was analyzed by a quantitative phenolic and flavonoid content with spectrophotometer method, and tested as antioxidant and antimicrobial activites. Antioxidant activity was performed by 2,2-diphenyl-1-picrilhidrazil (DPPH) method, while antimicrobial assay used pathogenic bacteria by disk-diffusion method. The results concluded that the stem of D. falcata plant showed a high content of phenolic and flavonoid compounds, very high antioxidant and moderate antimicrobial activities. It was also found that stems of D. falcata contain potential phenolic compounds that can be used as natural antioxidants and the treatment of various infections caused by microbes.

scholarly journals POTENTIAL ANTIOXIDANT AND CYTOTOXIC ACTIVITIES OF ARECA NUT (ARECA CATECHU LINN.) EXTRACT IN HUMAN ORAL SQUAMOUS CELL CARCINOMA AND KERATINOCYTE CELLS

2017 ◽  
Vol 10 (10) ◽  
pp. 286 ◽  
Author(s):  
Liza Meutia Sari ◽  
Gus Permana Subita ◽  
Elza Ibrahim Auerkari
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic ◽  
Cytotoxic Activities ◽  
Hacat Cells ◽  
Areca Nut ◽  
Flavonoid Content ◽  
Flavonoid Compounds ◽  
Areca Catechu ◽  
Phenolic And Flavonoid ◽  
Phenolic And Flavonoid Compounds

  Objectives: Areca (betel, Areca catechu) nuts have been reported to show high contents of phenolic and flavonoid compounds with associated antioxidant activity. This study was undertaken to assess the anticancer potential of areca nut extract on human squamous carcinoma (HSC)-3, HSC-2, and human keratinocyte (HaCat) cancer cell lines, and to elucidate the mechanism of its action.Methods: In this study, the areca nut extract was prepared in 96% ethanol and analyzed for the total phenolic and flavonoid content, 2,2-diphenyl-1- picrylhydrazyl (DPPH) assay, and 5-(3-carboxymethoxyphenyl)-2-(4,5-dimenthylthiazoly)-3-(4-sulfophenyl)tetrazolium inner salt assay.Results: The indicated total phenolic content of the areca nut extract was 80.3 mg tannic acid equivalent/g and total flavonoid content 238.5 mg catechin equivalent/g. The extract showed good scavenging activity against DPPH radical (efficient concentration 15.95±0.84 μg/ml). The extract showed higher toxicity on HSC-3 than HSC-2 cells, IC50 was 164.06 μg/ml in HSC-3 versus 629.50 μg/ml in HSC-2. The extract had no cytotoxic effect on HaCat cells.Conclusions: The antioxidant activity of the areca nut extract might be associated with the presence of high content of phenolic and flavonoid compounds. The extract showed selective cytotoxicity against HSC-3 and HSC-2 cells but not on HaCat cells. Instead, the extract induced high rate of proliferation in HaCat cells.

scholarly journals Total phenolic and flavonoid compounds, antioxidant and toxicity profile of extract and fractions of paku atai tuber (Angiopteris ferox Copel)

Food Research ◽  
2019 ◽  
pp. 734-740
Author(s):  
Nur S. ◽  
F. Mubarak ◽  
C. Jannah ◽  
D.A. Winarni ◽  
D.A. Rahman ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Crude Extract ◽  
Iron Reduction ◽  
Colorimetric Method ◽  
Total Phenolic ◽  
Toxicity Profile ◽  
Flavonoid Content ◽  
Ic50 Value ◽  
Phenolic And Flavonoid ◽  
Phenolic And Flavonoid Compounds

Paku atai plant (Angiopteris ferox Copel), specifically the tuber has been used empirically as an anticancer and antidote materials by Dayaknese in West Kutai, with limited scientific study. Thus, this research had been conducted to determine and evaluate the total phenolic, flavonoid content of this plant along with the antioxidant and toxicity profile. The research used several extracts’ solvents including extraction with ethanol (crude extract, CE) and subsequently partitioned with n-hexane (HF); ethyl acetate (EF) and ethanol aqueous (EAF). The resulted crude extract and fractions were then analyzed through colorimetric method to determine the phenolic and flavonoid total; with DPPH and FRAP to observe the antioxidant activity; and using BSLT method to evaluate the toxicity activity. The results showed that the EF fraction provided the strongest antioxidant activity with IC50 value of 13.79 μg/mL and iron reduction with FRAP value of 387.5±6.41 µM/g. However, a high correlation was observed between the antioxidant and the total phenolic content (r2 = 0.970 - 0.974) but little correlation in total flavonoid (r2 = 0.345 - 0.373). Furthermore, the EF (19.56±7.35) showed the highest toxicity activity followed by CE (22.42±2.10), HF (39.52±7.38), and EAF (41.75±5.10). Therefore, the paku atai tuber can be potentially developed as a natural antioxidant and anticancer material.

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