scholarly journals Comparison of Erythrocyte Membrane Lipid Profiles between NAFLD Patients with or without Hyperlipidemia

2020 ◽  
Vol 2020 ◽  
pp. 1-12
Author(s):  
Wenbin Chen ◽  
Shanshan Shao ◽  
Hu Cai ◽  
Jie Han ◽  
Tian Guo ◽  
...  
Keyword(s):  
Erythrocyte Membrane ◽  
Cell Function ◽  
Membrane Lipids ◽  
Weight Percent ◽  
Membrane Lipid ◽  
Lipid Profiles ◽  
Data Sets ◽  
Lipid Profiling ◽  
Nonalcoholic Fatty Liver ◽  
Lipid Species

Objectives. Nonalcoholic fatty liver disease (NAFLD) and hyperlipidemia (HL) are common metabolic disorders due to overnutrition and obesity. NAFLD is often associated with hyperlipidemia. The aim of this study was to identify and compare the erythrocyte membrane lipids profile in NAFLD patients with or without HL. Methods. A total of 112 subjects (with similar age and body mass index) were divided into four groups: (1) normal controls, (2) NAFLD alone, (3) HL alone, and (4) NAFLD combined with HL (NAFLD + HL). Lipid was extracted from the erythrocyte membrane, and lipid profiles of subjects were analyzed by liquid chromatography mass spectrometry (LC-MS). Results. Data sets from 103 subjects were adopted for lipidomic analysis. Significant changes of lipid species were observed in patient groups, especially in the HL group and NAFLD + HL group. The HL group showed increased level of most lipid species, and decreased level of most lipid species was observed in the NAFLD + HL group. The weight percent of myristic acid, stearic acid, erucic acid, and docosahexaenoic acid also showed distinct variation between different groups. Conclusions. NAFLD, HL, and NAFLD + HL all had an impact on lipid profiling of the erythrocyte membrane. The influence of NAFLD alone is less important compared with HL. Some lipids should be highlighted because of their specific role in cell function and systemic metabolism.

scholarly journals Lipid Remodeling Confers Osmotic Stress Tolerance to Embryogenic Cells during Cryopreservation

2021 ◽  
Vol 22 (4) ◽  
pp. 2174
Author(s):  
Liang Lin ◽  
Junchao Ma ◽  
Qin Ai ◽  
Hugh W. Pritchard ◽  
Weiqi Li ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Technology Development ◽  
Membrane Lipids ◽  
Species Conservation ◽  
Membrane Lipid ◽  
Cell Integrity ◽  
Embryogenic Cells ◽  
Osmotic Stress Tolerance ◽  
Lipid Species ◽  
Magnolia Officinalis

Plant species conservation through cryopreservation using plant vitrification solutions (PVS) is based in empiricism and the mechanisms that confer cell integrity are not well understood. Using ESI-MS/MS analysis and quantification, we generated 12 comparative lipidomics datasets for membranes of embryogenic cells (ECs) of Magnolia officinalis during cryogenic treatments. Each step of the complex PVS-based cryoprotocol had a profoundly different impact on membrane lipid composition. Loading treatment (osmoprotection) remodeled the cell membrane by lipid turnover, between increased phosphatidic acid (PA) and phosphatidylglycerol (PG) and decreased phosphatidylcholine (PC) and phosphatidylethanolamine (PE). The PA increase likely serves as an intermediate for adjustments in lipid metabolism to desiccation stress. Following PVS treatment, lipid levels increased, including PC and PE, and this effectively counteracted the potential for massive loss of lipid species when cryopreservation was implemented in the absence of cryoprotection. The present detailed cryobiotechnology findings suggest that the remodeling of membrane lipids and attenuation of lipid degradation are critical for the successful use of PVS. As lipid metabolism and composition varies with species, these new insights provide a framework for technology development for the preservation of other species at increasing risk of extinction.

Manipulating membrane lipid profiles to restore T-cell function in autoimmunity

2015 ◽  
Vol 43 (4) ◽  
pp. 745-751 ◽  
Author(s):  
Kirsty E. Waddington ◽  
Elizabeth C. Jury
Keyword(s):  
T Cell ◽  
Lipid Rafts ◽  
Lupus Erythematosus ◽  
Cell Function ◽  
Immune Cell ◽  
Membrane Lipids ◽  
Membrane Lipid ◽  
Plasma Membrane Lipid ◽  
Disease Systemic Lupus Erythematosus ◽  
Acyl Chains

Plasma membrane lipid rafts are heterogeneous cholesterol and glycosphingolipid (GSL)-enriched microdomains, within which the tight packing of cholesterol with the saturated-acyl chains of GSLs creates a region of liquid-order relative to the surrounding disordered membrane. Thus lipid rafts govern the lateral mobility and interaction of membrane proteins and regulate a plethora of signal transduction events, including T-cell antigen receptor (TCR) signalling. The pathways regulating homoeostasis of membrane cholesterol and GSLs are tightly controlled and alteration of these metabolic processes coincides with immune cell dysfunction as is evident in atherosclerosis, cancer and autoimmunity. Indeed, membrane lipid composition is emerging as an important factor influencing the ability of cells to respond appropriately to microenvironmental stimuli. Consequently, there is increasing interest in targeting membrane lipids or their metabolic control as a novel therapeutic approach to modulate immune cell behaviour and our recent work demonstrates that this is a promising strategy in T-cells from patients with the autoimmune disease systemic lupus erythematosus (SLE).

scholarly journals Galactolipid and Phospholipid Profile and Proteome Alterations in Soybean Leaves at the Onset of Salt Stress

2021 ◽  
Vol 12 ◽  
Author(s):  
Ailin Liu ◽  
Zhixia Xiao ◽  
Zhili Wang ◽  
Hon-Ming Lam ◽  
Mee-Len Chye
Keyword(s):  
Salt Stress ◽  
Membrane Lipids ◽  
Membrane Lipid ◽  
Salt Treatment ◽  
Short Term ◽  
Lipid Profiling ◽  
Soybean Seedlings ◽  
Proteomic Data ◽  
Before And After ◽  
Soybean Leaves

Salinity is a major environmental factor that constrains soybean yield and grain quality. Given our past observations using the salt-sensitive soybean (Glycine max [L.] Merr.) accession C08 on its early responses to salinity and salt-induced transcriptomic modifications, the aim of this study was to assess the lipid profile changes in this cultivar before and after short-term salt stress, and to explore the adaptive mechanisms underpinning lipid homeostasis. To this end, lipid profiling and proteomic analyses were performed on the leaves of soybean seedlings subjected to salt treatment for 0, 0.5, 1, and 2 h. Our results revealed that short-term salt stress caused dynamic lipid alterations resulting in recycling for both galactolipids and phospholipids. A comprehensive understanding of membrane lipid adaption following salt treatment was achieved by combining time-dependent lipidomic and proteomic data. Proteins involved in phosphoinositide synthesis and turnover were upregulated at the onset of salt treatment. Salinity-induced lipid recycling was shown to enhance jasmonic acid and phosphatidylinositol biosyntheses. Our study demonstrated that salt stress resulted in a remodeling of membrane lipid composition and an alteration in membrane lipids associated with lipid signaling and metabolism in C08 leaves.

Microscopy of membrane lipids: how precisely can we define their distribution?

2015 ◽  
Vol 57 ◽  
pp. 81-91 ◽  
Author(s):  
Sho Takatori ◽  
Toyoshi Fujimoto
Keyword(s):  
Membrane Lipids ◽  
Freeze Fracture ◽  
Practical Method ◽  
Membrane Lipid ◽  
Electron Microscopic ◽  
Functional Roles ◽  
Lipid Dynamics ◽  
Lipid Species ◽  
Quick Freezing ◽  
The Moment

Membrane lipids form the basic framework of biological membranes by forming the lipid bilayer, but it is becoming increasingly clear that individual lipid species play different functional roles. However, in comparison with proteins, relatively little is known about how lipids are distributed in the membrane. Several microscopic methods are available to study membrane lipid dynamics in living cells, but defining the distribution of lipids at the submicrometre scale is difficult, because lipids diffuse quickly in the membrane and most lipids do not react with aldehydes that are commonly used as fixatives. Quick-freezing appears to be the only practical method by which to stop the lipid movement instantaneously and capture the molecular localization at the moment of interest. Electron microscopic methods, using cryosections, resin sections, and freeze-fracture replicas are used to visualize lipids in quick-frozen samples. The method that employs the freeze-fracture replica is unique in that it requires no chemical treatment and provides a two-dimensional view of the membrane.

LIPID COMPOSITION OF CELLULAR MEMBRANES UNDER BIFIDUM-BAG PROBIOTIC IN GENTAMICIN-ASSOCIATED DYSBIOSIS

2019 ◽  
pp. 25-32
Author(s):  
V.A. Korolev ◽  
O.A. Medvedeva ◽  
A.D. Bogomazov ◽  
N.A. Verevkina ◽  
I.V. Korolev
Keyword(s):  
Erythrocyte Membrane ◽  
Broad Spectrum ◽  
Lipid Composition ◽  
Plasma Membranes ◽  
Membrane Lipids ◽  
Neutral Lipids ◽  
Membrane Lipid ◽  
The Body ◽  
Quantitative Composition ◽  
Lipid Spectrum

The erythrocyte membrane is a user-friendly model, since its structural is similar to that of molecular structure of plasma membranes. Therefore, the slightly corrected patterns of changes in the structure and functions of the erythrocyte membrane can be transferred to other membrane systems. Changes in the structure of membrane lipids under various factors are of great importance for the functional state of both the membranes themselves and the body as a whole. In diseases with severe hypoxic syndrome, changes in the membrane structure are the most obvious ones. These disorders can be observed under exposure to various drugs, namely, broad-spectrum antibiotics. The aim of the paper is to study the lipid composition of erythrocyte membranes under gentamicin-associated dysbiosis and to correct it with the B. Bifidum. Materials and Methods. The study was conducted on 60 BALB/c mice (18–20 g.). The animals were divided into three groups, 20 animals in each. The first group is a control one (intact mice). The second group consisted of animals with modeled gentamicin-associated dysbiosis. Animals of the third group were treated with Bifidum BAG Probiotic (21 days, once a day) after the formation of fixed drug dysbiosis. Traditional methods were used to determine the lipid composition of red blood cells. Chromatography was performed according to V.I. Krylov method. Results. To correct pathological conditions, the authors used Bifidum BAG probiotic, which consists of living active bifidobacteria B. bifidum, B. longum, and powerful plant antioxidant, dihydroquercetin. Administration of a broad-spectrum antibiotic (gentamicin) resulted in a significant change in the quantitative composition of neutral lipids and phospholipids. Intake of a complex probiotic led to the membrane lipid spectrum correction. Conclusion. It is established that Bifidum BAG probiotic leads to a normalization of the erythrocyte membrane lipid spectrum with gentamicin-associated dysbiosis, which may be associated with the antioxidant, membrane-stabilizing and antihypoxic effect of the drug. Keywords: dysbiosis, phospholipids, neutral lipids, erythrocyte membrane, Bifidum BAG. Эритроцитарная мембрана является удобным модельным объектом, так как имеет общие принципы строения с молекулярной структурой плазматических мембран, поэтому закономерности изменений структуры и функций мембраны эритроцитов с незначительной долей коррекции могут быть перенесены на другие мембранные системы. Изменения в структуре липидов мембран под влиянием различных факторов имеют большое значение для функционального состояния как самих мембран, так и организма в целом. При заболеваниях, которые протекают с выраженным гипоксическим синдромом, изменения структуры мембраны наиболее выражены. Эти нарушения могут наблюдаться при воздействии различных лекарственных препаратов, в т.ч. антибиотиков широкого спектра действия. Целью исследования явилось изучение состава липидов мембран эритроцитов в условиях гентамицинассоциированного дисбиоза и коррекции его комплексным препаратом «Бифидум БАГ». Материалы и методы. Исследование проведено на 60 мышах линии BALB/c с массой 18–20 г. Животные были разделены на три группы по 20 особей в каждой. Первая группа – контрольная (интактные мыши). Вторую группу составили животные, которым моделировали гентамицинассоциированный дисбиоз. Животные третьей группы интрагастрально получали комплексный пробиотик «Бифидум БАГ» в течение 21 дня 1 раз в сутки после формирования стойкого лекарственного дисбактериоза. Липидный состав эритроцитов определяли традиционными методами. Хроматографирование проводили по методу В.И. Крылова. Результаты. Для коррекции патологических состояний использовали комплексный препарат «Бифидум БАГ», в состав которого, помимо живых активных видов бифидобактерий B. bifidum и B. Longum, входит растительный антиоксидант – дигидрокверцетин. Применение антибиотика широкого спектра действия (гентамицина) привело к значительному изменению количественного состава нейтральных липидов и фосфолипидов. Введение комплексного пробиотика привело к коррекции спектра липидов мембран. Выводы. Установлено, что комплексный препарат «Бифидум БАГ» приводит к нормализации спектра липидов мембран эритроцитов при гентамицинассоциированном дисбиозе, что может быть связано с антиоксидантным, мембраностабилизирующим и антигипоксическим действием препарата. Ключевые слова: дисбиоз, фосфолипиды, нейтральные липиды, мембрана эритроцита, «Бифидум БАГ».

Membrane lipid diffusion and band 3 protein changes in human erythrocytes due to acute hypobaric hypoxia

1998 ◽  
Vol 275 (6) ◽  
pp. C1429-C1431 ◽  
Author(s):  
Gloria Celedón ◽  
Gustavo González ◽  
Carlos P. Sotomayor ◽  
Claus Behn
Keyword(s):  
Erythrocyte Membrane ◽  
Hypobaric Hypoxia ◽  
Protein Modification ◽  
Membrane Lipids ◽  
Band 3 ◽  
Membrane Lipid ◽  
Erythrocyte Membranes ◽  
Dodecanoic Acid ◽  
Band 3 Protein ◽  
Acute Hypobaric Hypoxia

Because it has been reported that hypoxia in rats may promote lipid peroxidation and other free radical reactions that could modify membrane lipids and proteins, the effect of acute hypobaric hypoxia on human erythrocyte membranes was investigated. 12-(1-Pyrene)dodecanoic acid fluorescent probe was used to assess short-range lateral diffusion status in the membrane bilayer. Membrane protein modification was detected by SDS-PAGE. Healthy young men were exposed for 20 min to the hypobaric hypoxia, simulating an altitude of 4,500 m. Under this condition, erythrocyte membrane lipids reached a state of higher lateral diffusivity with respect to normobaric conditions and membrane band 3 protein was modified, becoming more susceptible to membrane-bound proteinases. These observations suggest that acute hypobaric hypoxia may promote an oxidative stress condition in the erythrocyte membrane.

scholarly journals Defining how multiple lipid species interact with inward rectifier potassium (Kir2) channels

2020 ◽  
Vol 117 (14) ◽  
pp. 7803-7813 ◽  
Author(s):  
Anna L. Duncan ◽  
Robin A. Corey ◽  
Mark S. P. Sansom
Keyword(s):  
Plasma Membrane ◽  
Membrane Lipids ◽  
Membrane Lipid ◽  
Inward Rectifier ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Anionic Lipid ◽  
Lipid Interactions ◽  
Interaction Sites ◽  
Lipid Species

Protein–lipid interactions are a key element of the function of many integral membrane proteins. These potential interactions should be considered alongside the complexity and diversity of membrane lipid composition. Inward rectifier potassium channel (Kir) Kir2.2 has multiple interactions with plasma membrane lipids: Phosphatidylinositol (4, 5)-bisphosphate (PIP2) activates the channel; a secondary anionic lipid site has been identified, which augments the activation by PIP2; and cholesterol inhibits the channel. Molecular dynamics simulations are used to characterize in molecular detail the protein–lipid interactions of Kir2.2 in a model of the complex plasma membrane. Kir2.2 has been simulated with multiple, functionally important lipid species. From our simulations we show that PIP2interacts most tightly at the crystallographic interaction sites, outcompeting other lipid species at this site. Phosphatidylserine (PS) interacts at the previously identified secondary anionic lipid interaction site, in a PIP2concentration-dependent manner. There is interplay between these anionic lipids: PS interactions are diminished when PIP2is not present in the membrane, underlining the need to consider multiple lipid species when investigating protein–lipid interactions.

scholarly journals Criticality of plasma membrane lipids reflects activation state of macrophage cells

2019 ◽  
Author(s):  
Eugenia Cammarota ◽  
Chiara Soriani ◽  
Raphaelle Taub ◽  
Fiona Morgan ◽  
Jiro Sakai ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Critical Point ◽  
Membrane Lipids ◽  
Membrane Lipid ◽  
Membrane Receptors ◽  
Critical Conditions ◽  
Membrane Composition ◽  
Macrophage Cells ◽  
Lipid Species ◽  
Anti Inflammatory

AbstractSignalling is of particular importance in immune cells, and upstream in the signalling pathway many membrane receptors are functional only as complexes, co-locating with particular lipid species. Work over the last 15 years has shown that plasma membrane lipid composition is close to a critical point of phase separation, with evidence that cells adapt their composition in ways that alter the proximity to this thermodynamical point. Macrophage cells are a key component of the innate immune system, responsive to infections, regulating the local state of inflammation. We investigate changes in the plasma membrane’s proximity to the critical point, as a response to stimulation by various pro- and anti-inflammatory agents. Pro-inflammatory (IFN-γ, Kdo-LipidA, LPS) perturbations induce an increase in the transition temperature of the GMPVs; anti-inflammatory IL4 has the opposite effect. These changes recapitulate complex plasma membrane composition changes, and are consistent with lipid criticality playing a master regulatory role: being closer to critical conditions increases membrane protein activity.

Erythrocyte membrane lipid peroxidation and glycosylated hemoglobin in diabetes

Diabetes ◽  
1989 ◽  
Vol 38 (12) ◽  
pp. 1539-1543 ◽  
Author(s):  
S. K. Jain ◽  
R. McVie ◽  
J. Duett ◽  
J. J. Herbst
Keyword(s):  
Lipid Peroxidation ◽  
Erythrocyte Membrane ◽  
Glycosylated Hemoglobin ◽  
Membrane Lipid ◽  
Membrane Lipid Peroxidation

scholarly journals Profiling of Cerebrospinal Fluid Lipids and Their Relationship with Plasma Lipids in Healthy Humans

Metabolites ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 268
Author(s):  
Kosuke Saito ◽  
Kotaro Hattori ◽  
Shinsuke Hidese ◽  
Daimei Sasayama ◽  
Tomoko Miyakawa ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Total Protein ◽  
Plasma Lipids ◽  
Plasma Lipid ◽  
Lipid Profiles ◽  
Brain Diseases ◽  
Lipid Homeostasis ◽  
Total Protein Content ◽  
Lipid Profiling ◽  
Healthy Humans

Lipidomics provides an overview of lipid profiles in biological systems. Although blood is commonly used for lipid profiling, cerebrospinal fluid (CSF) is more suitable for exploring lipid homeostasis in brain diseases. However, whether an individual’s background affects the CSF lipid profile remains unclear, and the association between CSF and plasma lipid profiles in heathy individuals has not yet been defined. Herein, lipidomics approaches were employed to analyze CSF and plasma samples obtained from 114 healthy Japanese subjects. Results showed that the global lipid profiles differed significantly between CSF and plasma, with only 13 of 114 lipids found to be significantly correlated between the two matrices. Additionally, the CSF total protein content was the primary factor associated with CSF lipids. In the CSF, the levels of major lipids, namely, phosphatidylcholines, sphingomyelins, and cholesterolesters, correlated with CSF total protein levels. These findings indicate that CSF lipidomics can be applied to explore changes in lipid homeostasis in patients with brain diseases.

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