scholarly journals Optimization of Enzymatic Conditions of Sturgeon Muscles and Their Anti-Inflammatory Potential

2020 ◽  
Vol 2020 ◽  
pp. 1-12 ◽  
Author(s):  
Ruichang Gao ◽  
Yang Shen ◽  
Wanghui Shu ◽  
Fan Bai ◽  
Wengang Jin ◽  
...  
Keyword(s):  
Radical Scavenging ◽  
Degree Of Hydrolysis ◽  
Inhibition Rate ◽  
Liquid Ratio ◽  
Box Behnken Design ◽  
No Inhibition ◽  
Solid Liquid ◽  
Hydrolysis Of ◽  
Proinflammatory Cytokine Level ◽  
And Oxidative Stress

The objective of this study was to investigate the effects of different enzymolysis conditions on the NO inhibition rate and DH (degree of hydrolysis) of sturgeon hydrolysates (SH) prepared by Alcalase. The NO inhibition rate of 60.23% was attained under the optimum enzymolysis conditions as follows: pH 9.0, enzymolysis time of 4.92 h, enzymolysis temperature of 55°C, solid/liquid ratio of 1 : 20, and enzyme additive amount of 7674.22 U/g protein, which was well matched with the predicted value 61.44% of the Box–Behnken design model. After the ultrafiltration of SH, SH-3 (SH < 3 kDa) could significantly decrease the levels of NO and proinflammatory cytokine level IL-6. Also, we found that the obtained SH-3 contained good properties of emulsification and possessed good WHC and OHC. SH-3 demonstrated appreciable antioxidant potential on DPPH and ABTS radical scavenging activities. These results suggested that SH-3 derived from sturgeon muscles could potentially be used as a promising ingredient against inflammatory and oxidative stress-associated diseases.

scholarly journals RSM-Modeling and Optimization of High Titer Functional Xylo-Oligosaccharides Production by Edible Gluconic Acid Catalysis

2021 ◽  
Author(s):  
Yuanjie Gu ◽  
Jianming Guo ◽  
Xin Zhou ◽  
Yong Xu
Keyword(s):  
Reaction Temperature ◽  
Gluconic Acid ◽  
Acid Catalysis ◽  
High Titer ◽  
Liquid Ratio ◽  
High Concentration ◽  
Box Behnken Design ◽  
Aldonic Acid ◽  
Solid Liquid ◽  
Hydrolysis Of

Abstract Xylo-oligosaccharides as a functional prebiotic have great value in food, feed fields. Previous studies have shown that organic acids catalyze the hydrolysis of xylan-rich sources for the production of xylo-oligosaccharides. In this study, gluconic acid of edible aldonic acid, generated xylo-oligosaccharides via hydrolysis of xylan from corncob. In order to maximize the efficiency of xylo-oligosaccharide production, a model was designed and optimal conditions were determined by Box-Behnken design-based response surface methodology. The developed process resulted in a maximum xylo-oligosaccharides yield of 57.73% using 4.6% gluconic acid at 167°C for 28 min, which was similar to the predicted value and fitted models of xylo-oligosaccharides production. The results showed that the reaction temperature was crucial to xylo-oligosaccharides production, and by-product yields (xylose and furfural) could be effectively controlled by both reaction temperature and time. In addition, 44.87 g/L XOS was achieved by decreasing the solid-liquid ratio. Overall, the described process may be a preferred option for future high concentration xylo-oligosaccharides production.

scholarly journals Preparation of Antioxidant Enzymatic Hydrolysates from Honeybee-Collected Pollen Using Plant Enzymes

2010 ◽  
Vol 2010 ◽  
pp. 1-5 ◽  
Author(s):  
Margarita D. Marinova ◽  
Bozhidar P. Tchorbanov
Keyword(s):  
Radical Scavenging ◽  
Total Phenolic ◽  
Degree Of Hydrolysis ◽  
Enzymatic Hydrolysates ◽  
Phenolic Contents ◽  
Scavenging Capacity ◽  
Plant Enzymes ◽  
Hydrolysis Of ◽  
Proline Iminopeptidase ◽  
Dietary Food

Enzymatic hydrolysates of honeybee-collected pollen were prepared using food-grade proteinase and aminopeptidases entirely of plant origin. Bromelain from pineapple stem was applied (8 mAU/g substrate) in the first hydrolysis stage. Aminopeptidase (0.05 U/g substrate) and proline iminopeptidase (0.03 U/g substrate) from cabbage leaves (Brassica oleracea var. capitata), and aminopeptidase (0.2 U/g substrate) from chick-pea cotyledons (Cicer arietinum L.) were involved in the additional hydrolysis of the peptide mixtures. The degree of hydrolysis (DH), total phenolic contents, and protein contents of these hydrolysates were as follows: DH (about 20–28%), total phenolics (15.3–27.2 μg/mg sample powder), and proteins (162.7–242.8 μg/mg sample powder), respectively. The hydrolysates possessed high antiradical scavenging activity determined with DPPH (42–46% inhibition). The prepared hydrolysates of bee-collected flower pollen may be regarded as effective natural and functional dietary food supplements due to their remarkable content of polyphenol substances and significant radical-scavenging capacity with special regard to their nutritional-physiological implications.

Antioxidant Properties of Microalgae Protein Hydrolysates Prepared by Neutral Protease Digestion

2014 ◽  
Vol 707 ◽  
pp. 149-153 ◽  
Author(s):  
Xiao Hu ◽  
Xian Qing Yang ◽  
Lai Hao Li ◽  
Yan Yan Wu ◽  
Wan Ling Lin ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Protein Hydrolysates ◽  
Neutral Protease ◽  
Degree Of Hydrolysis ◽  
Protease Digestion ◽  
Hydrolysis Of

Microalgae protein hydrolysates (MPH) were obtained by enzymatic hydrolysis of defatted microalgae meal using neutral protease. The protein recovery, degree of hydrolysis, and the antioxidant activities of the hydrolysates were investigated. The results demonstrated that hydrolysates prepared by neutral protease at 50 °C for 4 h exhibited the strongest antioxidant activity. Under these conditions, the 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl radical scavenging activity and the reducing power of the hydrolysates were 68.3%, 50.8% and 1.303, respectively.

scholarly journals Calcium peroxide pretreatment to facilitate the delignification and enzymatic hydrolysis of wheat straw

2021 ◽  
Author(s):  
Youshan Sun ◽  
Xuyang Zhang ◽  
Fei Wang ◽  
Meiyan Wang
Keyword(s):  
Enzymatic Hydrolysis ◽  
Wheat Straw ◽  
Complex Structure ◽  
Raw Material ◽  
Liquid Ratio ◽  
Enzymatic Digestibility ◽  
Calcium Peroxide ◽  
Glucose Recovery ◽  
Solid Liquid ◽  
Hydrolysis Of

Abstract Calcium peroxide (CaO2) pretreatment was employed to remove lignin and subsequently facilitate enzymatic digestibility of wheat straw. An optimal condition was obtained at 130°C for 10 min with 0.35 g CaO2/g dried material of wheat straw and a 1:8 solid-liquid ratio. Under this condition, 57.8% of initial lignin, 7.2% of initial glucan, and 30.6% of initial xylan were removed from CaO2 pretreatment, respectively, meanwhile, a glucose recovery of 90.6 % and a xylose recovery of 65.9 % were obtained from the subsequent enzymatic hydrolysis of treated wheat straw, respectively. CaO2 pretreatment was proved to be a very effective method in delignification and improving enzymatic digestibility. Compared to raw material, the complex structure of lignocellulose was drastically disrupted with a wide emergence of scaly bulges and fully exposed microfibers, which still retained in the solid.

scholarly journals Effects of High-Intensity Ultrasound Pretreatment on Structure, Properties, and Enzymolysis of Soy Protein Isolate

Molecules ◽  
2019 ◽  
Vol 24 (20) ◽  
pp. 3637 ◽  
Author(s):  
Fei Zhao ◽  
Xuemei Liu ◽  
Xiuzhen Ding ◽  
Haizhou Dong ◽  
Wentao Wang
Keyword(s):  
High Intensity ◽  
Near Infrared ◽  
Soybean Protein ◽  
Soy Protein Isolate ◽  
Radical Scavenging ◽  
Protein Isolate ◽  
Degree Of Hydrolysis ◽  
Soybean Protein Isolate ◽  
Near Infrared Spectra ◽  
Hydrolysis Of

The objective of this study was to investigate the effects of different high-intensity ultrasonication (HIU) pretreatment on the structure and properties of soybean protein isolate (SPI) as well as enzymatic hydrolysis of SPI by bromelain and antioxidant activity of hydrolysates. The HIU-treated SPI fractions showed a decrease in the proportion of α-helices and β-turns and an increase in the content of β-sheets and random coils based on Fourier-transform infrared spectroscopy. Near-infrared spectra and fluorescence spectra analyses provided support for the changes in secondary and tertiary structures of SPI after ultrasound treatment. The particle size of SPI decreased from 217.20 nm to 141.23 nm and the absolute zeta potential increased. Scanning electron microscopy showed that HIU treatment changed apparent morphology. Dynamic and static light scattering of ultrasonicated samples showed that SPI structure had changed from hard-sphere to hollow-sphere or polydisperse and monodisperse gaussian coils. HIU pretreatment significantly increased the hydroxyl-radical scavenging and the degree of hydrolysis of the SPI hydrolysates.

Study on Dilute Sulfuric Acid Hydrolysis of Corn Stover

2011 ◽  
Vol 236-238 ◽  
pp. 313-316
Author(s):  
Jun Ping Zhu ◽  
Jian Lu Ma ◽  
Xiao Chen Liu ◽  
Biao Zhang ◽  
Wei Jie Xu ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Sulfuric Acid ◽  
Corn Stover ◽  
Optimal Conditions ◽  
Liquid Ratio ◽  
Factors Influencing ◽  
Dilute Sulfuric Acid ◽  
Hydrolysis Temperature ◽  
Solid Liquid ◽  
Hydrolysis Of

The object of this research is to investigate the factors influencing the hydrolysis of corn stover by dilute sulfuric acid and the types of sugars existed in hydrolysate. Experiment results showed that the optimal conditions were hydrolysis temperature 120°C, stover size 20-40 mesh, soak time 36h, reaction time 100 min, H2SO4concentration 1.5% and solid-liquid ratio 1:8. Sugars gained in the hydrolysate were xylose 3.0%, glucose 0.71%, mannose 0.12% and galactose 0.32% determined by capillary electrophoresis.

scholarly journals ANTIOXIDANT ACTIVITY OF PROTEIN HYDROLYSATE PRODUCED FROM TUNA EYE (Thunnus sp.) BY ENZYMATIC HYDROLYSIS

2018 ◽  
Vol 21 (3) ◽  
pp. 522 ◽  
Author(s):  
Dewi Mutamimah ◽  
Bustami Ibrahim ◽  
Wini Trilaksani
Keyword(s):  
Antioxidant Activity ◽  
Enzymatic Hydrolysis ◽  
Protein Hydrolysate ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Degree Of Hydrolysis ◽  
Protective Agent ◽  
Ic50 Value ◽  
Canning Industry ◽  
Hydrolysis Of

Tuna (Thunnus sp.) by-products from frozen loin and canning industry especially the eye is rich in proteins and in lipids consisting of polyunsaturated fatty acids (PUFA). That requires protective agent (antioxidant) to inhibit the oxidation naturally present and predicted to be protein peptides. Enzymatic hydrolysis of protein is an appropriate method to produce bioactive peptide with such nutraceutical/pharmaceutical function such as an antioxidant peptide. This study aimed to produce protein hydrolysate having a function as anwith an antioxidant activity from eye of tuna through enzymatic hydrolysis and determining the antioxidant activity by DPPH methods. Protein soluble content of tuna’s eye protein hydrolysate (TEPH) ranged from 59.98±0.130 to 94.90±0.002%. The degree of hydrolysis (DH) of TEPH was about 9.10±0.28 to 16.14±0.09%. The highest inhibition of DPPH radical scavenging activity was 93.57±0.05% (at 5 mg/mL) was obtained with a DH of 11.35±0.002% at the concentration 0.1% of papain for 6 hours hydrolysis. The IC50 value of was 1.08±0.008 mg/mL

The Preparation of Micro-Molecular Dextran in Sub-Critical Water/CO2

2013 ◽  
Vol 712-715 ◽  
pp. 502-505
Author(s):  
Shu Qiong Liao ◽  
Xiao Yu Peng ◽  
Xue Wang Zhang ◽  
Ke Lin Huang ◽  
Ben Wang ◽  
...  
Keyword(s):  
Reaction Time ◽  
Reaction Temperature ◽  
Temperature Reaction ◽  
Liquid Ratio ◽  
Reaction Conditions ◽  
Reaction Pressure ◽  
Optimum Reaction ◽  
Ft Ir ◽  
Solid Liquid ◽  
Hydrolysis Of

Micro-molecular dextran was prepared in sub-critical water/CO2 by hydrolysis of dextran20. The obtained products were mainly characterized by FT-IR and GPC. Furthermore, the reaction temperature, reaction time, reaction pressure, solid-liquid radio and stirring speed were systematically investigated during the work. The optimum reaction conditions are as follows: the reaction temperature was 160°C; the reaction time was 60 min; the reaction pressure was 2.5MPa; the solid-liquid ratio was 0.6 and the stirring speed was 300r/min.

scholarly journals OPTIMIZATION OF HYDROTHERMAL PRETREATMENT FOR ENZYMATIC HYDROLYSIS OF BANANA PSEUDO STEM USING RESPONSE SURFACE METHODOLOGY

2019 ◽  
Vol 2 (4) ◽  
pp. 32-38
Author(s):  
Isadora Ferreira da Silva ◽  
José Humberto Queiroz ◽  
José Maria Rodrigues Da Luz
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Hydrothermal Pretreatment ◽  
Regression Equations ◽  
Liquid Ratio ◽  
Glucose Yield ◽  
Sugar Release ◽  
Cellulose Digestibility ◽  
Solid Liquid ◽  
Hydrolysis Of

In this study different conditions of hydrothermal pretreatment were evaluated for conversion of banana pseudo stem to fermentable sugars. A Central Composite Design (CCD) was used to obtain regression equations in function of the following variables: solid/liquid ratio (1/10; 1/12.5; 1/15), temperature (170 °C, 190 °C, 210 °C) and reaction time (10 min, 15 min, 20 min). The cellulose digestibility improved in all conditions tested, with maximum digestibility achieved at 210 °C for 10 minutes and 1/15 of solid/liquid ratio. The Glucose yield at optimal conditions was 58.4 g/kg with an excellent recovery of cellulose of 98%. Thus, the hydrothermal pretreatment demonstrated to be an effective process in increasing cellulose concentration and producing fermentable sugar from recalcitrant lignocellulosic biomass. Furthermore, based on the design response surface methodology, an optimum condition of each pretreatment could be obtained from the statistical models built. All the variables studied influenced the enzymatic sugar release.

Optimization of Extraction Technology of Favonoids from Kandelia Candel Leaves by Using Response Surface Methodology

2013 ◽  
Vol 704 ◽  
pp. 332-337
Author(s):  
Yan Ping Ji ◽  
Cheng Ping Li ◽  
Xing Xu ◽  
Lan Tang ◽  
Qian Tong
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Ethanol Concentration ◽  
Extraction Time ◽  
Kandelia Candel ◽  
Liquid Ratio ◽  
Extraction Technology ◽  
Box Behnken Design ◽  
Extraction Parameters ◽  
Solid Liquid

This work is to extract flavonoids using ethanol refluxing method from the Kandelia candel leaves which are rich in flavonoids. Ethanol concentration, solid-liquid ratio, temperature and extraction time on the extraction rate were investigated by the single factor experiments. Box-Behnken design (BBD) based on response surface methodology (RSM) was adopted to optimize the parameters. The results showed the best extracting rate was more than 7.74%, when the extraction parameters were ethanol concentration 60%, extraction time 0.5 h and temperature 80°C.

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