scholarly journals Corneal Epithelial Removal with a Newly Designed Epithelial Brush

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Ho Seok Chung ◽  
Seung Hwan Moon ◽  
Soon-Suk Kang ◽  
Minseop Kim ◽  
Hun Lee ◽  
...  
Keyword(s):  
Inflammatory Cell ◽  
Rabbit Model ◽  
The Other ◽  
Nuclear Antigen ◽  
Corneal Epithelial ◽  
Defect Area ◽  
Scope Of Application ◽  
Hematoxylin And Eosin ◽  
Conventional Methods ◽  
Proliferating Cell

This study aimed to evaluate and compare the effectiveness of a newly developed epithelial removal brush with conventional methods in a rabbit model of corneal epithelial defects. The corneal epithelia of thirty-seven rabbits were removed by three different methods including blades (blade group), newly developed epithelial brushes (Ocu group), and conventional rotating brushes (Amo group). The defect area was measured with light microscopy immediately and at 4, 18, 24, and 50 hours after removal. Corneas were obtained immediately and at 24 and 50 hours and subjected to hematoxylin and eosin (H&E) and immunofluorescence staining using proliferating cell nuclear antigen (PCNA) and phosphorylated heat shock protein 27 (pHSP27) antibodies. The residual stromal surface was observed by scanning electron microscopy (SEM). In the Ocu group, epithelia were significantly recovered at 18, 24, and 50 hours compared with immediately after removal, and in the blade and Amo groups, epithelia were significantly recovered only at 50 hours after epithelial removal. The expression levels of PCNA and pHSP27 did not differ among three groups. There was significantly more inflammatory cell infiltration in the blade group than in the other groups. SEM showed a more regular and uniform residual stromal surface in the Ocu group than in the other groups. The newly developed epithelial brush showed better polishing ability and led to earlier significant epithelial recovery and a more regular and uniform stromal surface than conventional methods in this rabbit model of epithelial defects. Accumulation of clinical data is expected to expand the scope of application of new brushes for laser surface ablation.

Cell Proliferation as Assessed by Expression of Proliferating Cell Nuclear Antigen or the Uptake of Bromodeoxyuridine in a Rabbit Model of Leg-lengthening

1996 ◽  
Vol 09 (03) ◽  
pp. 95-100 ◽  
Author(s):  
G. Li ◽  
A. H. R. W. Simpson ◽  
J. Kenwright ◽  
J. T. Triffitt
Keyword(s):  
Cell Proliferation ◽  
Proliferating Cell Nuclear Antigen ◽  
Rabbit Model ◽  
Experimental System ◽  
Nuclear Antigen ◽  
Positive Staining ◽  
Leg Lengthening ◽  
Staining Index ◽  
Proliferating Cell ◽  
Distraction Rate

SummaryAn experimental model of leg lengthening has been used to study the cellular responses of the regenerating bone to different rates of distraction. Cell proliferation were assessed by detection of proliferating cell nuclear antigen using a monoclonal antibody, PC10. The technique was verified by comparison with bromodeoxyuridine uptake and subsequent detection with specific antibody (Bu20A). The positive staining index (PSI) was calculated for a variety of tissues and the PC10 PSI was greater than that of Bu20A, as described by the expression: PC10 PSI = 1.6 Bu20A PSI + 12.9, with a correlation coefficient 0.79. The results suggest that PC10 may be used as an alternative marker to assess cell proliferation in rabbit regenerating bone tissue. In addition, the rate of cell proliferation during leg-lengthening was found to reach a maximum at a distraction rate of 0.7 mm/day without further change at higher rates.Cell proliferation was assessed in an experimental system of leg-lengthening by two separate methods. The presence of proliferating cell nuclear antigen or the uptake of bromodeoxyuridine were determined immuno-histochemically. Both methods indicated cell proliferation during leg-lengthening reaches a maximum at a distraction rate of 0.7 mm/day.

scholarly journals Alterations of the extracellular matrix of lung during zinc deficiency

2011 ◽  
Vol 108 (1) ◽  
pp. 62-70 ◽  
Author(s):  
Verónica S. Biaggio ◽  
Natalia R. Salvetti ◽  
María V. Pérez Chaca ◽  
Susana R. Valdez ◽  
Hugo H. Ortega ◽  
...  
Keyword(s):  
Proliferating Cell Nuclear Antigen ◽  
Nitrosative Stress ◽  
Health Interventions ◽  
The Other ◽  
Male Rats ◽  
Nuclear Antigen ◽  
Zn Deficiency ◽  
Oxidative And Nitrosative Stress ◽  
Fibrotic Process ◽  
Proliferating Cell

Suboptimal intake of Zn is one of the most common nutritional problems worldwide. Previously, we have shown that Zn deficiency (ZD) produces oxidative and nitrosative stress in the lung of rats. We analyse the effect of moderate ZD on the expression of several intermediate filaments of the cytoskeleton, as well as the effect of restoring Zn during the refeeding period. Adult male rats were divided into three groups: Zn-adequate control (CO) group; ZD group; Zn-refeeding group. CerbB-2 and proliferating cell nuclear antigen (PCNA) expression was increased in the ZD group while the other parameters did not change. During the refeeding time, CerbB-2, cytokeratins, vimentin and PCNA immunostaining was higher than that in the CO group. The present findings indicate that the overexpression of some markers could lead to the fibrotic process in the lung. Perhaps ZD implications must be taken into account in health interventions because an inflammation environment is associated with ZD in the lung.

scholarly journals Study On The Biomechanical Properties Of Rabbit Venous Arterialization

2019 ◽  
Author(s):  
Yuhao Zhang ◽  
Pei Niu ◽  
Zhimin Zhang ◽  
Xiaolong Niu ◽  
Wenzeng Shen ◽  
...  
Keyword(s):  
Early Stage ◽  
Artery Bypass ◽  
Rabbit Model ◽  
Biomechanical Properties ◽  
Arterial System ◽  
Nuclear Antigen ◽  
Minimal Damage ◽  
Alpha Actin ◽  
And Control ◽  
Proliferating Cell

Abstract Objective : To investigate the mechanisms underlying restenosis following coronary artery bypass grafting using bridging veins.Method : We established a rabbit model of venous arterialisation, by transplanting veins into the arterial system as bridging vessels and investigated vessel tensile mechanical and histomorphological properties. Result : Control vein elasticity (k = 16.20) was less than that of the control artery (k = 58.04; P < 0.05), and vein walls were thinner. Following venous arterialisation, proliferating cell nuclear antigen and alpha-actin were upregulated and vein walls thickened (P < 0.05), with elasticity after venous arterialisation (k = 86.26) significantly higher than that of control veins (P < 0.05). Conclusion : This indicates that venous intima is damaged by high pressure following arterialisation, resulting in gradual restenosis, with thickening of the venous intima and an increase in vessel elasticity. Clinically, there is potential to repeat these experiments to determine the elastic extremum of the great saphenous vein and control the pressure in the lumen of this vessel, to ensure minimal damage to the intima before anastomosis, thereby facilitating improvement of long-term patency rates following vein bridge surgery. Whether the increase in venous bridge elasticity after venous arterialisation can be controlled, with the aim of preventing early-stage restenosis, warrants investigation.

scholarly journals Effect of Ovarian Steroids on Colonic Epithelial Cell Proliferation and Apoptosis in Rats

2007 ◽  
Vol 76 (4) ◽  
pp. 605-612
Author(s):  
K. Altunbas ◽  
A. Yagci ◽  
A. Bulbul ◽  
A. Sevimli ◽  
V. Özdemir
Keyword(s):  
The Other ◽  
Nuclear Antigen ◽  
Proliferation Index ◽  
Sprague Dawley ◽  
Colon Crypt ◽  
Crypt Epithelium ◽  
Ovx Rats ◽  
Proliferation And Apoptosis ◽  
The Mean ◽  
Proliferating Cell

The aim of this study was to investigate the effects of steroid hormones on proliferation and apoptosis in the colon crypt epithelium. The research was conducted on adult ovariectomized (Ovx) rats (Sprague Dawley). Ovx rats were injected for 15 days with 0.2 ml of sesame oil (control; C), or 17β-oestradiol (10 μg/d; E), or progesterone (2 mg/d; P), or E + P. Proliferative activity in the colon was assessed by using proliferating cell nuclear antigen (PCNA) antibody. The proliferation index (PI), the number of PCNA positive cells divided by the total number of cells counted in the crypt column multiplied by 100, was calculated. PI was lower in the hormonetreated groups, especially in group P compared to that in group C. The apoptotic index (AI), the mean number of apoptotic cells, was detected by active caspase 3 immunoreactivity per crypt in the colon. AI was lower in the colon crypt epithelium of group E than that of the other groups. However, AI in the colon crypt epithelium in groups P and E + P was higher than that of both group E and group C. In addition, the colon crypt size (the number of epithelial cells lining one side of 10 well-oriented, longitudinally cut crypts) was considerably lower in group E than that of the other groups. In conclusion, we showed that the decrease of AI in group E was balanced by progesterone; the decrease of PI in group P was also depressed by oestrogen.

scholarly journals Histomorphometric and Immunohistochemical Study Comparing the Effect of Diabetes Mellitus on the Acini of the Sublingual and Submandibular Salivary Glands of Albino Rats

2020 ◽  
Vol 8 (A) ◽  
pp. 49-54
Author(s):  
Sarah Yasser ◽  
Ahmed Shon
Keyword(s):  
Diabetes Mellitus ◽  
Salivary Glands ◽  
Proliferating Cell Nuclear Antigen ◽  
Immunohistochemical Study ◽  
Nuclear Antigen ◽  
Albino Rats ◽  
Weighted Mean ◽  
Submandibular Salivary Glands ◽  
Hematoxylin And Eosin ◽  
Proliferating Cell

AIM: This study was designed to compare the effect of diabetes on the mucous and seromucous acini of the sublingual (SLG) and the submandibular (SMG) salivary glands of albino rats, respectively. METHODS: Twenty male albino rats were assigned into two groups; control and diabetic. Three months following the induction of diabetes mellitus (DM), both the SMG and the SLG glands were removed, randomly sectioned and stained with hematoxylin and eosin to estimate the volume-weighted mean volume of the acini of both glands together with examining their morphology. Furthermore, immunohistochemistry was done to examine the expression of the proliferating cell nuclear antigen (PCNA) in both of them. RESULTS: We found that, unlike the SMG acinar cells, diabetes appeared not to affect both the morphology and the volume of the SLG acini. Interestingly, PCNA expression in diabetic SMG glands acini was significantly higher than diabetic SLG glands acini. Furthermore, we found that the expression pattern of PCNA was significantly higher between the control and diabetic groups in both glands. CONCLUSION: We concluded that the mucous acini of the SLG glands are less affected by the oxidative damage induced by DM.

scholarly journals Chitosan nanoparticles as a promising candidate for liver injury induced by 2-nitropropane: Implications of P53, iNOS, VEGF, PCNA, and CD68 pathways

2021 ◽  
Vol 104 (2) ◽  
pp. 003685042110118
Author(s):  
Sameerah Shaheen ◽  
Maha M Arafah ◽  
Aliah R Alshanwani ◽  
Laila Mohammed Fadda ◽  
Ahlam M Alhusaini ◽  
...  
Keyword(s):  
Chitosan Nanoparticles ◽  
Nuclear Antigen ◽  
Vascular Endothelial ◽  
Sod Activity ◽  
Tnf Α ◽  
Current Article ◽  
Hematoxylin And Eosin ◽  
Cluster Of Differentiation ◽  
Endothelial Growth Factor ◽  
Proliferating Cell

The current article was designed to assess the role of chitosan nanoparticles (CNPs) in the management of hepatic injury induced by the hepatocarcinogen 2-nitropropane (2-NP). Rats were divided into three groups. The first group served as a control, the second group was injected with 2-NP, while the third group was treated with CNPs 1 h before 2-NP injection every other day for 4 weeks. The 2-NP injection upregulated serum AST and ALT activities, as well as hepatic TNF- α, IL-6, and MDA levels and the expression of vascular endothelial growth factor (VEGF) and caspase-3, whereas GSH contents and SOD activity were decreased. Immunohistochemistry investigations revealed that the hepatic protein expression of collagen I, inducible nitric oxide synthetase, proliferating cell nuclear antigen, cluster of differentiation, and p53 were upregulated. hematoxylin and eosin (H&E) and Masson’s trichrome stains supported the previous parameters, and CNPs ameliorated most of the previous biochemical parameters. CNPs achieved promising results in the limitation of 2-NP hepatotoxicity.

Avian Collagen Is Useful for the Construction of Skin Equivalents

2017 ◽  
Vol 204 (5-6) ◽  
pp. 261-269 ◽  
Author(s):  
Hailan Li ◽  
Hye-Young Yun ◽  
Kwang Jin Baek ◽  
Nyoun Soo Kwon ◽  
Hye-Ryung Choi ◽  
...  
Keyword(s):  
Proliferating Cell Nuclear Antigen ◽  
Immunohistochemical Staining ◽  
Nuclear Antigen ◽  
Integrin Β1 ◽  
Skin Equivalents ◽  
Test Models ◽  
Hematoxylin And Eosin ◽  
Alternative Test ◽  
Proliferating Cell ◽  
Do So

As a result of restrictions on animal experimentation, improved skin equivalents (SEs) are needed as alternative test models. This work investigated the effects of avian collagen on the construction of SEs, and to the best of our knowledge is the first study to do so. Hematoxylin and eosin and immunohistochemical staining were used to analyze the SEs. In models containing avian collagen as a dermal equivalent (DE) ingredient, fibroblast proliferation increased by about 60% relative to the control model. Immunohistochemical staining showed that the expression of proliferating cell nuclear antigen (PCNA) and p63 increased in the avian collagen models, while the expression of involucrin, integrin α6, and integrin β1 remained unchanged. Next, DEs were cryopreserved to allow the easier creation of SEs. Keratinocytes were seeded on thawed DEs, and SEs were constructed. Avian collagen increased the viability of DEs relative to the control. Furthermore, avian collagen increased the expression of PCNA and p63 in keratinocytes on thawed DEs. The results indicate that DEs containing avian collagen can be thawed as needed after cryopreservation. Avian collagen can improve the construction of SEs and be used as part of a dermal kit for SE construction.

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