Determination of the Chemical Composition and Antimicrobial Activity of Lavatera thuringiaca L. Medicinal Herb Material Extracted under Subcritical Conditions by the Liquid Carbon Dioxide Method

This article presents the composition of the components of Lavatera thuringiaca L. (Malvaceae Juss. family), which has a certain antibacterial effect. The plant collection was carried out in the Shamalgan gorge of Mountain Range of the Trans-Ili Alatau in the territory of the Karasay district of the Almaty region, in the flowering phase. A CO2 extract of the aboveground part of the medicinal plant Lavatera thuringiaca L. was obtained under subcritical conditions and, for the first time, studied for its component composition and antimicrobial activity. Determination of the chemical composition of the extract was carried out by gas chromatography/mass spectrometry (GC/MS). To identify the obtained mass spectra, we used the Wiley 7th edition and the NIST’02 data library. To determine the antimicrobial and antifungal activity, standard test strains of microorganisms were used: Staphylococcus aureus ATCC 6538-P, Escherichia coli ATCC 8739, Pseudomonas aeruginosa ATCC 9027, Candida albicans ATCC 10231, Streptococcus pneumonia ATCC 660, Klebsiella pneumoniae ATCC 700603, Staphylococcus haemolyticus, and Staphylococcus saprophyticus. In the composition of thick CO2Lavatera thuringiaca L. extract, the content of 31 components was proven: spathulenol 6.97%, pulegone 5 08%, cis-β-farnesene 7.63%, verbenone 1.93%, α-bisabolol oxide B 9.65%, bisabolol oxide A 8.26%, α-bisabolol 1.36%, linolenic acid, ethyl ether 3.15%, phytol 2.49%, herniarin 5.61%, linolenic acid 9.38%, linoleic acid 6.95%, myristic acid 2.33%, and elaidic acid 2.57%. Antimicrobial activity studies have shown that the CO2 extract of Lavatera thuringiaca L. has a pronounced effect against clinically significant microorganisms: Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans, Streptococcus pneumonia, Klebsiella pneumoniae, Staphylococcus haemolyticus, and Staphylococcus saprophyticus. During testing, the method of serial dilutions proved that the extract of Lavatera thuringiaca L. has a bactericidal effect on Staphylococcus aureus at a concentration of 0.83 μg/μl, on Escherichia coli at a concentration of 3.33 μg/μl, on Pseudomonas aeruginosa at a concentration of 0.83 μg/μl, on Streptococcus pneumoniae at a concentration of 1.67 μg/μl, on a clinical isolate of Staphylococcus haemolyticus at a concentration of 26.65 μg/μl, on Staphylococcus saprophyticus at a concentration of 6.67 μg/μl, and against Klebsiella pneumoniae at a concentration of 13.36 μg/μl. The test result showed that the extract also has fungicidal activity against the test culture of Candida albicans at a concentration of 0.21 μg/μl. At tests, the disc diffusion method proved that the extract has antimicrobial activity with high values of the growth suppression zone exceeding 15 mm. The zones of growth retardation of the test strains were 19.33 ± 1.15 for Staphylococcus aureus; 17.33 ± 3.21 for Escherichia coli; 15.67 ± 0.57 for Pseudomonas aeruginosa; 20.0 ± 1.0 for Streptococcus pneumoniae; 16.0 ± 2.64 for Klebsiella pneumoniae; 15.0 ± 1.0 for Staphylococcus saprophyticus, and 22.0 ± 1.73 for Candida albicans. In relation to the clinical isolate of Staphylococcus haemolyticus, the extract has a bacteriostatic effect.

Download Full-text

Antimicrobial activity of soft and purified propolis extracts

Medicina ◽

10.3390/medicina44120122 ◽

2008 ◽

Vol 44 (12) ◽

pp. 977 ◽

Cited By ~ 6

Author(s):

Alvydas Pavilonis ◽

Algirdas Baranauskas ◽

Ligita Puidokaitė ◽

Žaneta Maželienė ◽

Arūnas Savickas ◽

...

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Antimicrobial Activity ◽

Bacillus Subtilis ◽

Candida Albicans ◽

Phenolic Compounds ◽

Klebsiella Pneumoniae ◽

Gram Negative ◽

Propolis Extract

Objective. To evaluate the antimicrobial activity of soft and purified propolis extracts. Study object and methods. Antimicrobial activity of soft and purified propolis extracts was determined with reference cultures of Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 33499, Pseudomonas aeruginosa ATCC 27853, Proteus mirabilis ATCC 12459, Bacillus subtilis ATCC 6633, Bacillus cereus ATCC 8035, and fungus Candida albicans ATCC 60193. Microbiological tests were performed under aseptic conditions. Minimum inhibitory concentration (MIC) – the highest dilution of preparation (the lowest concentration of preparation) that suppresses growth of reference microorganisms – was determined. Results. Concentration of phenolic compounds in soft propolis extract that possesses antimicrobial activity against gram-positive (Staphylococcus aureus, Enterococcus faecalis) and gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa, and Proteus mirabilis) is 0.587±0.054 mg and 0.587±0.054–0.394±0.022 mg (P>0.05) and in purified propolis extract – 0.427±0.044 mg and 0.256±0.02 mg (P>0.05). Klebsiella pneumoniae is most resistant to soft propolis extract when the concentration of phenolic compounds is 1.119± 0.152 mg and to purified propolis extract when the concentration of phenolic compounds is 1.013±0.189 mg (P>0.05). Spore-forming Bacillus subtilis bacteria are more sensitive to soft and purified propolis extracts when the concentration of phenolic compounds is 0.134±0.002 mg and 0.075±0.025 mg, respectively, and Bacillus cereus – when the concentration is 0.394±0.022 mg and 0.256±0.02 mg (P>0.05). Sensitivity of fungus Candida albicans to soft and purified propolis extracts is the same as Bacillus subtilis. Encapsulated bacterium Klebsiella pneumoniae is most resistant to antimicrobial action of soft and purified propolis extracts as compared with gram-positive Staphylococcus aureus and Enterococcus faecalis bacteria (P<0.05), gram-negative Escherichia coli, Pseudomonas aeruginosa, and Proteus mirabilis (P<0.05), sporeforming Bacillus subtilis and Bacillus cereus bacteria (P<0.05), and fungus Candida albicans (P<0.05). There is no statistically significant difference between antimicrobial effect of soft propolis extract and purified propolis extract on gram-positive bacteria, gram-negative bacteria, spore-forming bacteria, encapsulated bacteria, and Candida fungus. Conclusions. Soft and purified propolis extracts possess antimicrobial activity. They could be recommended as natural preservatives in the manufacture of pharmaceutical products.

Download Full-text

Investigation of the Antimicrobial Activity of Rhaponticum (Rhaponticum Carthamoides D.C. Iljin) and Shrubby Cinquefoil (Potentilla Fruticosa L.)

Medicina ◽

10.3390/medicina47030024 ◽

2011 ◽

Vol 47 (3) ◽

pp. 24 ◽

Cited By ~ 2

Author(s):

Vilma Jurkštienė ◽

Alvydas Pavilonis ◽

Daiva Garšvienė ◽

Algirdas Juozulynas ◽

Laimutė Samsonienė ◽

...

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Antimicrobial Activity ◽

Bacillus Subtilis ◽

Candida Albicans ◽

Klebsiella Pneumoniae ◽

Bacillus Cereus ◽

Potentilla Fruticosa ◽

Rhaponticum Carthamoides

The aim of the study was to determine antimicrobial activity of rhaponticum and shrubby cinquefoil extracts. Material and Methods. Ethanol extract from the leaves of rhaponticum (Rhaponticum carthamoides D.C. Iljin) and shrubby cinquefoil (Potentilla fruticosa L.) was produced at the Department of Food Technology, Kaunas University of Technology. The antimicrobial activity of the viscous extract or rhaponticum and shrubby cinquefoil was evaluated using standard microorganism cultures (bacteria Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 33499, Pseudomonas aeruginosa ATCC 27853, Proteus mirabilis ATCC 12459, Bacillus subtilis ATCC 6633, Bacillus cereus ATCC 8035 and fungi Candida albicans ATCC 60193). The minimum inhibitory concentration (MIC) of the examined preparations was determined. Results. Both studied preparations – rhaponticum (Rhaponticum carthamoides D.C. Iljin) and shrubby cinquefoil (Potentilla fruticosa L.) – demonstrated similar antimicrobial activity. The highest sensitivity to the studied preparations was observed in microbes with eukaryotic cell structure: Candida albicans, which is a fungus, and a spore-forming prokaryotic bacterium, Bacillus cereus. The highest resistance was observed in Escherichia coli and Klebsiella pneumoniae. Conclusions. The studied preparations – viscous extracts of rhaponticum and shrubby cinquefoil – are substances with antimicrobial activity against gram-positive (Staphylococcus aureus and Enterococcus faecalis) and gram-negative (Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Proteus mirabilis) bacteria, spore-forming bacteria (Bacillus subtilis and Bacillus cereus), and fungi (Candida albicans).

Download Full-text

Determination of antimicrobial activity of alcohol extractions of bark and buds of Quercus robur L.

Aspirantskiy Vestnik Povolzhiya ◽

10.17816/2072-2354.2020.20.1.158-163 ◽

2020 ◽

Vol 20 (1-2) ◽

pp. 152-157

Author(s):

Nikolay A. Ryabov ◽

Vladimir A. Kurkin ◽

Vitaliy M. Ryzhov ◽

Artem V. Lyamin ◽

Alexander V. Zhestkov ◽

...

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Antimicrobial Activity ◽

Candida Albicans ◽

Quercus Robur ◽

Antimicrobial Effect ◽

Raw Material ◽

Microbiological Analysis

Antimicrobial activity of alcoholic extractions from the aboveground organs like bark and buds of Quercus robur L. was studied. The determination of the minimum inhibitory concentration (MIC) was performed with the use of double serial dilution in broth. The following microorganisms were used as test cultures: Bacillus cereus, Candida albicans, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus. It has been revealed that alcoholic extractions of the bark of Quercus robur have the widest spectrum of antibacterial activity in relation to Pseudomonas aeruginosa, Escherichia coli and Candida albicans. Results of microbiological analysis show that alcoholic extractions from the bark and buds have an antimicrobial effect on all of these strains with a single dilution (except for antimicrobial activity against Staphylococcus aureus with 70% extractions of bark of Quercus robur). In particular, relatively high antimicrobial activity was observed in alcoholic extracts of all buds concentrations against Candida albicans at a dilution of 2, 4 and 8 times and Escherichia coli at a dilution of 4, 8 and 16 times. The greatest antimicrobial effect against Pseudomonas aeruginosa has extracts from the bark and buds in all the studied concentrations of alcohol. A significant antimicrobial effect against Escherichia coli has 40% of extractions from the buds of Quercus robur when diluted by 2, 4, 8, 16, 32 and 64 times. The results can be used as justification for the introduction of a new type of raw material that is buds of Quercus robur, as well as drugs based on it in the State Pharmacopoeia of the Russian Federation as antibacterial phytopharmaceuticals.

Download Full-text

СИНТЕЗ ТА ДОСЛІДЖЕННЯ АНТИМІКРОБНОЇ АКТИВНОСТІ ДЕЯКИХ ПІРАЗОЛЗАМІЩЕНИХ 7H-[1,2,4]ТРИАЗОЛО[3,4-B][1,3,4]ТІАДІАЗИНІВ

Фармацевтичний часопис ◽

10.11603/2312-0967.2021.1.11986 ◽

2021 ◽

pp. 5-13

Author(s):

I. I. Myrko ◽

T. I. Chaban ◽

V. V. Ogurtsov ◽

V. S. Matiychuk

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Candida Albicans ◽

Drug Discovery ◽

Klebsiella Pneumoniae ◽

Acinetobacter Baumannii ◽

Cryptococcus Neoformans ◽

Antimicrobial Drug

Мета роботи. Здійснити синтез деяких нових піразолзаміщених 7H-[1,2,4]триазоло[3,4-b][1,3,4]тіадіазинів та провести дослідження антимікробних властивостей синтезованих сполук. Матеріали і методи. Органічний синтез, ЯМР-спектроскопія, елементний аналіз, фармакологічний скринінг. Результати й обговорення. У результаті взаємодії eтил (2Z)-хлоро(фенілгідразоно)ацетатів з ацетилацетоном було отримано етил 4-ацетил-5-метил-1-феніл-1H-піразол-3-карбоксилати. Зазначені сполуки піддали бромуванню, що дозволило одержати цільові бромкетони. Синтезовані на даній стадії етил 1-арил-4-(бромацетил)-5-метил-1Н-піразол-3-карбоксилати було введено у взаємодію з 4-аміно-5-арил(гетарил)-2,4-дигідро-3Н-1,2,4-триазол-3-тіонами з подальшим формуванням 1,3,4-тіадіазольного циклу та отриманням відповідних етил 1-арил-4-{3-арил(гетарил)-7H-[1,2,4]триазоло[3,4-b][1,3,4]тіадіазин-6-іл)}-5-метил-1H-піразол-3-карбоксилатів. Структура синтезованих сполук підтверджена даними елементного аналізу та ЯМР спектроскопією. В рамках міжнародного проекту "The Community for Antimicrobial Drug Discovery" (CO-ADD) за підтримки Wellcome Trust (Великобританія) і університету Квінсленда (Австралія) для синтезованих сполук здійснено скринінг антимікробної активності. Як тестові мікроорганізми використовували п'ять штамів бактерій: Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 700603, Acinetobacter baumannii ATCC 19606, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 43300 та двох штамів грибків: Candida albicans ATCC 90028 і Cryptococcus neoformans ATCC 208821. Встановлено, що досліджувані сполуки виявляють різноманітну дію, від практично повної її відсутності до виразного антимікробного ефекту. Висновки. Здійснено синтез 12 нових етил 1-арил-4-{3-арил(гетарил)-7H-[1,2,4]триазоло[3,4-b][1,3,4]тіадіазин-6-іл)}-5-метил-1H-піразол-3-карбоксилатів. Зазначені речовини отримані шляхом взаємодії відповідних етил 1-арил-4-(бромацетил)-5-метил-1Н-піразол-3-карбоксилатів з 4-аміно-5-арил(гетарил)-2,4-дигідро-3Н-1,2,4-триазол-3-тіонами. Дослідження антимікробної активності синтезованих сполук демонструють потенціал пошуку антимікробних агентів серед зазначеного класу сполук.

Download Full-text

Componentes químicos y actividad antimicrobiana del aceite esencial de Lasiocephalus ovatus (Asteraceae) que crece en Ecuador

Acta Biológica Colombiana ◽

10.15446/abc.v25n1.75728 ◽

2020 ◽

Vol 25 (1) ◽

pp. 22-28

Author(s):

Liliana Margarita Araujo Baptista ◽

Katerine Vimos-Sisa ◽

Rosa Cruz-Tenempaguay ◽

Félix Falconí-Ontaneda ◽

Luis Rojas-Fermín ◽

...

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Candida Albicans ◽

Klebsiella Pneumoniae ◽

Enterococcus Faecalis

Ecuador es uno de los países más ricos en biodiversidad y endemismo del mundo, y cerca de 3200 especies de plantas tienen usos medicinales. El objetivo de esta investigación fue evaluar la composición química y el efecto antimicrobiano del aceite esencial de Lasiocephalus ovatus Schltdl. (Asteraceae) colectada en la provincia de Chimborazo, Ecuador. Las partes aéreas de L. ovatus fueron sometidas a hidrodestilación para obtener el aceite esencial, el cual fue analizado mediante cromatografía de gases acoplada a espectrometría de masas. La actividad fue evaluada frente a cinco bacterias y una levadura usando la técnica de microdilución en caldo en microplacas de 96 pozos. El rendimiento del aceite fue de 0,05 % y 27 compuestos fueron identificados, representando 95,45 % de la composición total con un elevado contenido de monoterpenos oxigenados (52,17 %). Los compuestos mayoritarios fueron alcanfor (40,48 %) y 1,2,5,5-tetrametil-1,3-ciclopentadieno (11,90 %), seguido por p-menta-1,5-dien-8-ol (5,23 %) y 1,6-dimetilhepta-1,3,5-trieno (4,69 %). Las bacterias más sensibles a la acción del aceite fueron Staphylococcus aureus ATCC 25923 y Escherichia coli ATCC 25922 con concentraciones mínimas inhibitorias de 200-400 μg/mL y bactericidas de 800 μg/mL. La inhibición antimicrobiana frente a las bacterias Enterococcus faecalis ATCC 29212, Pseudomonas aeruginosa ATCC 27853, Klebsiella pneumoniae ATCC 700603 y la levadura Candida albicans ATCC 10231 fue baja, con un rango de concentración mínima inhibitoria de 800 a 6400 μg/mL. Este reporte representa un primer análisis de la actividad antimicrobiana del aceite de L. ovatus, por lo tanto, una contribución importante al estudio del género Lasiocephalus.

Download Full-text

Prevalência e perfil de sensibilidade a antimicrobianos de uropatógenos em pacientes atendidos no laboratório escola do Centro Universitário de Brasília (UniCEUB) - Distrito Federal

Programa de Iniciação Científica - PIC/UniCEUB - Relatórios de Pesquisa ◽

10.5102/pic.n0.2019.7642 ◽

2021 ◽

Author(s):

Bárbara Clemente Ribeiro ◽

Hian Delfino Ferreira Da Silva ◽

Luís Eduardo Santos Barros

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Klebsiella Pneumoniae ◽

Enterococcus Faecalis ◽

Proteus Mirabilis ◽

Streptococcus Agalactiae ◽

Staphylococcus Saprophyticus ◽

Staphylococcus Haemolyticus ◽

E 52 ◽

Distrito Federal

O uso indiscriminado de antibióticos no tratamento das Infecções do Trato Urinário (ITU) funciona como pressão seletiva ao surgimento de resistência bacteriana aos antimicrobianos. Deste modo, este estudo teve como objetivo determinar a prevalência de uroculturas positivas e o perfil antimicrobiano dos organismos encontrados em pacientes ambulatoriais atendidos no Laboratório Escola no Centro Universitário de Brasília (UniCEUB) no Distrito Federal. Foi realizado um estudo transversal retrospectivo, no qual teve como base a análise dos registros laboratoriais de uroculturas realizadas no período entre Agosto de 2017 e Dezembro de 2019. Os selecionados como critérios de inclusão: apresentar crescimento microbiano igual ou superior a 100.000 UFC/mL (Unidades Formadoras de Colônia) na urina, possuir resultado de identificação do agente patogênico e o resultado do teste de sensibilidade aos antimicrobianos. Foram excluídos da pesquisa pacientes com os dados cadastrais incompletos. Durante o período analisado, foram realizados 2.436 exames de urocultura, destes 2.281 foram excluídos do estudo: 2.252 por não apresentarem crescimento bacteriano e 29 não apresentaram ficha cadastral completa ou crescimento microbiano inferior ao selecionado para a pesquisa. Por fim, foram selecionadas para este trabalho 155 uroculturas positivas, dentre as quais 92% (142) pertenciam a pacientes do sexofemino e 8% (13) ao sexo masculino, com idade média de 48 e 52 anos respectivamente. Dentre a população analisada, foi observado positividade das uroculturas para os seguintes microrganismos: Escherichia coli (78,71%), Klebsiella pneumoniae (7,74%), Proteus mirabilis (4,52%), Streptococcus agalactiae (3,87%), Staphylococcus saprophyticus (1,94%), Staphylococcus haemolyticus e Enterococcus faecalis (1,29%) e Staphylococcus aureus (0,65%). A Escherichia coli foi o microrganismo que apresentou maior prevalência dentre as uroculturas analisadas, de modo que foi avaliado também o perfil de sensibilidade aos antimicrobianos para tal patógeno. Com exceção da ampicilina e da cefalotina, todos os β-lactâmicos testados apresentaram sensibilidade superior a 80%. A resistência da Escherichia coli a ampicilina foi de 50,82%, enquanto a resistência média aos cefalosporínicos foi de aproximadamente 11,5%, 5,74% a gentamicina. Os derivados quinolônicos apresentaram as maiores taxas de resistência: entre 24,59% e 40,98%. Ao utilizar a associação Trimetoprim e Sulfametoxazol, foi observado 29,51% de resistência. Nas amostras analisadas, não houve resistência aos carbapenêmicos. Com base nos dados avaliados, conclui-se que a Escherichiacoli permanece como o principal microrganismo causador de ITU’s em pacientes ambulatoriais. Com base nisto se faz necessário o planejamento de um esquema terapêutico eficaz, visto que o microrganismo apresenta algum grau de resistência a diversos antimicrobianos utilizados na prática clínica.

Download Full-text

Microorganisms Isolated from Hospital Environmental Surfaces in Akure Metropolis, Ondo State, Nigeria

Journal of Advances in Microbiology ◽

10.9734/jamb/2019/v15i330104 ◽

2019 ◽

pp. 1-8

Author(s):

O. G. Palmer ◽

A. K. Onifade

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Candida Albicans ◽

Aspergillus Niger ◽

Klebsiella Pneumoniae ◽

Streptococcus Pyogenes ◽

Bacterial Isolates ◽

Environmental Surfaces ◽

Ondo State

Nosocomial infection is a rising problem in developing countries. Microorganisms Isolated from three private and public hospital environmental surfaces in Akure Metropolis, Ondo State, Nigeria were investigated in this study. Bacterial and fungal organisms were isolated and compared among the three hospitals. The study revealed that bacteria were the most predominant microorganisms found in the hospital environmental surfaces than fungi. Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae and Bacillus cereus were the bacterial isolates while fungi include Aspergillus fumigatus, Aspergillus niger and Candida albicans. Staphylococcus aureus was found to be predominant bacteria but Aspergillus funmigatus was the predominant fungi. The result showed that the microbial loads of the public hospitals were higher than that of the private hospitals. The bacteria load of the male ward was found to be higher than that of the female ward while the fungal loads of each of the hospital environmental surfaces of female were higher than that of the male. The study revealed that bacteria were the most predominant microorganisms found in the hospital environment than fungi. Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae and Bacillus cereus were the bacterial isolates while fungi include Aspergillus fumigatus, Aspergillus niger and Candida albicans. Staphylococcus aureus was found to be predominant bacteria. All the hospital environmental surfaces were contaminated with one or more microorganisms in the course of the research.

Download Full-text

Antimicrobial activity of bioactive component from flower of linum capitatum kit

Facta universitatis - series Physics Chemistry and Technology ◽

10.2298/fupct0401073i ◽

2004 ◽

Vol 3 (1) ◽

pp. 73-77 ◽

Cited By ~ 1

Author(s):

Slavica Ilic ◽

Sandra Konstantinovic ◽

Zoran Todorovic

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Antimicrobial Activity ◽

Candida Albicans ◽

Aspergillus Niger ◽

Bioactive Components ◽

Bioactive Component ◽

Bacillus Subtilus ◽

Simplex Virus

Different extracts containing bioactive components and etheric oil of the flowers of Linum capitation kit. (Linacea) of Serbian origin were tested for an Antimicrobial activity against four bacteria (Staphylococcus aureus Escherichia coli, Bacillus subtilus, Pseudomonas aeruginosa), one mold (Aspergillus niger) and one yeast (Candida albicans). The isolated Flavonoids were also tested against Staphylococcus aureus, Escherichia coli Bacillus anhtracis, Pseudomonas aeruginosa, Aspergillus niger, Candida albicans and Herpes simplex virus type.

Download Full-text

Potencial antimicrobiano, citotóxico e leishmanicida do extrato em diclorometano das partes aéreas de Mitracarpus frigidus (Rubiaceae)

Revista Eletrônica de Farmácia ◽

10.5216/ref.v17.54330 ◽

2020 ◽

Vol 17 (1) ◽

Author(s):

Rodrigo Luiz Fabri ◽

Paula Spagnol Da Cunha ◽

Lara Melo Campos ◽

Ari Sérgio de Oliveira Lemos ◽

Elaine Soares Coimbra ◽

...

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Candida Albicans ◽

Klebsiella Pneumoniae ◽

Salmonella Typhimurium ◽

Bacillus Cereus ◽

Cryptococcus Neoformans ◽

Artemia Salina ◽

Shigella Sonnei

Este presente estudo teve como objetivo investigar o potencial antimicrobiano, leishmanicida e citotóxico do extrato em diclorometano das partes aéreas de Mitracarpus frigidus. Além de identificar as principais classes de metabólitos especiais desse extrato. Para realização dos ensaios biológicos foram testadas nove linhagens de microrganismos (Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella typhimurium, Shigella sonnei, Klebsiella pneumoniae, Escherichia coli, Bacillus cereus, Candida albicans e Cryptococcus neoformans), duas espécies de Leishmanias (L. amazonensis e L. chagasi). A citotoxicidade foi testada frente ao microcrustáceo Artemia salina. Os resultados mostraram que o extrato em diclorometano apresentou expressiva atividades leishmanicida, frente a ambas formas promastigotas das espécies de Leishmanias, e antimicrobiana, principalmente contra B. cereus e C. neoformans. O ensaio de bioautografia identificou que as classes que possuem atividade antifúngica foram alcaloides e triterpenos. Os resultados apresentados abrem perspectivas para o futuro isolamento, purificação e identificação das substâncias bioativas de Mitracarpus frigidus que possam ser utilizadas no tratamento de infecções microbianas e leishmanioses.

Download Full-text

Prevalência de microrganismos em culturas de catéter

Revista de Ciências Médicas e Biológicas ◽

10.9771/cmbio.v17i2.25061 ◽

2018 ◽

Vol 17 (2) ◽

pp. 147

Author(s):

Nathalia Calabresi ◽

Leyde Daiane de Peder ◽

Heloise Skiavine Madeira ◽

Bruna Larissa Nascimento ◽

Denise Michelle Indras ◽

...

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Candida Albicans ◽

Klebsiella Pneumoniae ◽

Acinetobacter Baumanii

Os cateteres intravenosos são de uso amplamente difundido em hospitais e por estarem em contato direto com a circulação sanguínea, tornam-se um potencial risco para a ocorrência de contaminação e infecção local e sistêmica, aumentando a mortalidade, o tempo de internação e consequentemente custos hospitalares. O presente estudo teve como objetivo realizar levantamento epidemiológico da prevalência de bactérias em pontas de cateteres provenientes de sete hospitais do município de Cascavel - Paraná. Foi realizado um estudo transversal por meio da coleta de dados em laudos emitidos por um laboratório clínico privado do mesmo município, entre os anos de 2011 a 2014. Verificou-se positividade em 21,68% dos laudos analisados (248). O grupo dos bacilos Gram negativos (BGN) foi o mais prevalente, sendo as principais bactérias encontradas Escherichia coli e Klebsiella pneumoniae (84 laudos), seguidos de BGN não-fermentadores (79 laudos) e cocos Gram positivos (67 laudos). Leveduras foram encontradas em 18 laudos. Staphylococcus aureus apresentaram uma resistência de 31,81% para oxacilina, sendo para Staphylococcus coagulase-negativa resistência de 86,96%. Klebsiella pnemoniae apresentou discreta resistência para ertapenem (9,52%), imipenem (7,14%) e meropenem (7,14%), sugerindo presença de carbapenemase. Espécies de Enterobacter foram resistentes a diversos antimicrobianos, não constatado resistência aos carbapenêmicos ertapenem, imipenem e meropenem. Acinetobacter baumanii e Pseudomonas aeruginosa apresentaram elevada resistência para imipenem e meropenem (77,14% e 45,16%; 80% e 51,61%, respectivamente). Candida albicans apresentou 100% de suscetibilidade aos antifúngicos utilizados. Esses resultados demonstram a grande importância no manejo eficiente do controle de infecção hospitalar e uso responsável de antimicrobianos por hospitais.

Download Full-text